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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1051. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
1052. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
1053. DNA amount calculation for PCR - (reply: 14)
1054. ChIP primer design - (reply: 1)
1055. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
1056. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
1057. weird QPCR curves - (reply: 2)
1058. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
1059. Questions regarding RT-PCR optimization - (reply: 2)
1060. Problems with semi-quatitative PCR - (reply: 1)
1061. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
1062. Long-amplicon QPCR of mtDNA - (reply: 5)
1063. PCR reaction calculation - (reply: 2)
1064. Qiagen rotorgene syber green kit reaction volume - (reply: 1)
1065. missing nt at the 5' end - site mutagensis using NEB kit (reply: 1)
1066. no increase in fluorescence in my real time PCR - (reply: 3)
1067. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
1068. Roche second derivative maximum - can be treated according to Pfaffl? (reply: 1)
1069. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
1070. Pooled RNA from healthy controls - delta-delta Ct method (reply: 1)
1071. Probe Dilution Problem = 100uM to 5ng/ul? + Final Volume for FISH - Probe dilution and FISH - Advise on volume for FISH hibridization buff (reply: 2)
1072. PCR, using gel extracted band as a template - (reply: 4)
1073. Question of nucleic acid extraction - (reply: 1)
1074. Multiplex problems - (reply: 6)
1075. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
1076. Q-PCR and RT-PCR - (reply: 3)
1077. inter-run calibration - (reply: 4)
1078. Delta Delta Ct method for multiple reference genes? - (reply: 1)
1079. FAM and VIC labelling - (reply: 2)
1080. Lost cause?? - I made a silly (reply: 4)