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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1051. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
1052. Roche second derivative maximum - can be treated according to Pfaffl? (reply: 1)
1053. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
1054. Pooled RNA from healthy controls - delta-delta Ct method (reply: 1)
1055. Probe Dilution Problem = 100uM to 5ng/ul? + Final Volume for FISH - Probe dilution and FISH - Advise on volume for FISH hibridization buff (reply: 2)
1056. PCR, using gel extracted band as a template - (reply: 4)
1057. Question of nucleic acid extraction - (reply: 1)
1058. Multiplex problems - (reply: 6)
1059. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
1060. Q-PCR and RT-PCR - (reply: 3)
1061. inter-run calibration - (reply: 4)
1062. Delta Delta Ct method for multiple reference genes? - (reply: 1)
1063. FAM and VIC labelling - (reply: 2)
1064. Lost cause?? - I made a silly (reply: 4)
1065. how to use delta-delta Ct method? - when no expression in healthy controls! (reply: 2)
1066. confusing PCR - (reply: 1)
1067. difference between PCR primers and sequencing primers - (reply: 1)
1068. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
1069. making gel electrophoresis - (reply: 1)
1070. housekeeping genes in PCR - (reply: 1)
1071. Question about RealTime PCR Primer Design - (reply: 1)
1072. Primers stopped working!? - PCR Primers (reply: 6)
1073. NFK beta primers - (reply: 4)
1074. final PCR product - need clarifications (reply: 1)
1075. Trying to amplify the 3' end of Prolyl endopeptidse gene from cDNA.. need su - Hi, I'm trying to sequence a gene from the cDNA of an insect (reply: 2)
1076. Can I use Hot Start Taq polimerase for any target? - (reply: 6)
1077. Ct value and interpretation - (reply: 3)
1078. 16S rDNA - Bacteria identification (reply: 1)
1079. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
1080. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)