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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1051. DNA detection limit of the PCR - Calculating detection limits (reply: 4)
1052. cDNA synthesis - (reply: 3)
1053. 2 rounds PCR got problem - (reply: 2)
1054. PCR help minus strand - Primer design (reply: 1)
1055. cDNA contamination, pls help! - (reply: 1)
1056. Smear in long distance PCR - (reply: 39)
1057. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
1058. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
1059. can contaminating gDNA explain this? - (reply: 1)
1060. TOUCH DOWN PCR - (reply: 2)
1061. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
1062. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
1063. PCR stopped working - After changing buffer (reply: 5)
1064. how to determine the amount of cDNA for qPCR - (reply: 5)
1065. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)
1066. Standardization of HRM for methylation analysis - (reply: 4)
1067. PCR protocol questions! - (reply: 2)
1068. standard curve for qPCR - (reply: 3)
1069. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
1070. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
1071. DNA amount calculation for PCR - (reply: 14)
1072. ChIP primer design - (reply: 1)
1073. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
1074. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
1075. weird QPCR curves - (reply: 2)
1076. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
1077. Questions regarding RT-PCR optimization - (reply: 2)
1078. Problems with semi-quatitative PCR - (reply: 1)
1079. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
1080. Long-amplicon QPCR of mtDNA - (reply: 5)