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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1051. PCR help minus strand - Primer design (reply: 1)
1052. cDNA contamination, pls help! - (reply: 1)
1053. Smear in long distance PCR - (reply: 39)
1054. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
1055. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
1056. can contaminating gDNA explain this? - (reply: 1)
1057. TOUCH DOWN PCR - (reply: 2)
1058. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
1059. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
1060. PCR stopped working - After changing buffer (reply: 5)
1061. how to determine the amount of cDNA for qPCR - (reply: 5)
1062. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)
1063. Standardization of HRM for methylation analysis - (reply: 4)
1064. PCR protocol questions! - (reply: 2)
1065. standard curve for qPCR - (reply: 3)
1066. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
1067. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
1068. DNA amount calculation for PCR - (reply: 14)
1069. ChIP primer design - (reply: 1)
1070. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
1071. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
1072. weird QPCR curves - (reply: 2)
1073. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
1074. Questions regarding RT-PCR optimization - (reply: 2)
1075. Problems with semi-quatitative PCR - (reply: 1)
1076. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
1077. Long-amplicon QPCR of mtDNA - (reply: 5)
1078. PCR reaction calculation - (reply: 2)
1079. Qiagen rotorgene syber green kit reaction volume - (reply: 1)
1080. missing nt at the 5' end - site mutagensis using NEB kit (reply: 1)