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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1051. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
1052. Nested LA PCR - Any success? (reply: 3)
1053. Unpredictablity of PCR product - (reply: 5)
1054. total cDNA amplification by PCR - (reply: 2)
1055. Detection limit of a conventional PCR - (reply: 2)
1056. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
1057. RNAi showing upregulation via QPCR - Help needed (reply: 3)
1058. DNA detection limit of the PCR - Calculating detection limits (reply: 4)
1059. cDNA synthesis - (reply: 3)
1060. 2 rounds PCR got problem - (reply: 2)
1061. PCR help minus strand - Primer design (reply: 1)
1062. cDNA contamination, pls help! - (reply: 1)
1063. Smear in long distance PCR - (reply: 39)
1064. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
1065. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
1066. can contaminating gDNA explain this? - (reply: 1)
1067. TOUCH DOWN PCR - (reply: 2)
1068. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
1069. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
1070. PCR stopped working - After changing buffer (reply: 5)
1071. how to determine the amount of cDNA for qPCR - (reply: 5)
1072. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)
1073. Standardization of HRM for methylation analysis - (reply: 4)
1074. PCR protocol questions! - (reply: 2)
1075. standard curve for qPCR - (reply: 3)
1076. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
1077. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
1078. DNA amount calculation for PCR - (reply: 14)
1079. ChIP primer design - (reply: 1)
1080. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)