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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1051. Pooled RNA from healthy controls - delta-delta Ct method (reply: 1)
1052. Probe Dilution Problem = 100uM to 5ng/ul? + Final Volume for FISH - Probe dilution and FISH - Advise on volume for FISH hibridization buff (reply: 2)
1053. PCR, using gel extracted band as a template - (reply: 4)
1054. Question of nucleic acid extraction - (reply: 1)
1055. Multiplex problems - (reply: 6)
1056. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
1057. Q-PCR and RT-PCR - (reply: 3)
1058. inter-run calibration - (reply: 4)
1059. Delta Delta Ct method for multiple reference genes? - (reply: 1)
1060. FAM and VIC labelling - (reply: 2)
1061. Lost cause?? - I made a silly (reply: 4)
1062. how to use delta-delta Ct method? - when no expression in healthy controls! (reply: 2)
1063. confusing PCR - (reply: 1)
1064. difference between PCR primers and sequencing primers - (reply: 1)
1065. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
1066. making gel electrophoresis - (reply: 1)
1067. housekeeping genes in PCR - (reply: 1)
1068. Question about RealTime PCR Primer Design - (reply: 1)
1069. Primers stopped working!? - PCR Primers (reply: 6)
1070. NFK beta primers - (reply: 4)
1071. final PCR product - need clarifications (reply: 1)
1072. Trying to amplify the 3' end of Prolyl endopeptidse gene from cDNA.. need su - Hi, I'm trying to sequence a gene from the cDNA of an insect (reply: 2)
1073. Can I use Hot Start Taq polimerase for any target? - (reply: 6)
1074. Ct value and interpretation - (reply: 3)
1075. 16S rDNA - Bacteria identification (reply: 1)
1076. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
1077. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
1078. gene expressional analysis - (reply: 1)
1079. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
1080. Troubleshooting help: Why do my amplification curves look like this? - (reply: 8)