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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1051. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
1052. Long-amplicon QPCR of mtDNA - (reply: 5)
1053. PCR reaction calculation - (reply: 2)
1054. Qiagen rotorgene syber green kit reaction volume - (reply: 1)
1055. missing nt at the 5' end - site mutagensis using NEB kit (reply: 1)
1056. no increase in fluorescence in my real time PCR - (reply: 3)
1057. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
1058. Roche second derivative maximum - can be treated according to Pfaffl? (reply: 1)
1059. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
1060. Pooled RNA from healthy controls - delta-delta Ct method (reply: 1)
1061. Probe Dilution Problem = 100uM to 5ng/ul? + Final Volume for FISH - Probe dilution and FISH - Advise on volume for FISH hibridization buff (reply: 2)
1062. PCR, using gel extracted band as a template - (reply: 4)
1063. Question of nucleic acid extraction - (reply: 1)
1064. Multiplex problems - (reply: 6)
1065. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
1066. Q-PCR and RT-PCR - (reply: 3)
1067. inter-run calibration - (reply: 4)
1068. Delta Delta Ct method for multiple reference genes? - (reply: 1)
1069. FAM and VIC labelling - (reply: 2)
1070. Lost cause?? - I made a silly (reply: 4)
1071. how to use delta-delta Ct method? - when no expression in healthy controls! (reply: 2)
1072. confusing PCR - (reply: 1)
1073. difference between PCR primers and sequencing primers - (reply: 1)
1074. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
1075. making gel electrophoresis - (reply: 1)
1076. housekeeping genes in PCR - (reply: 1)
1077. Question about RealTime PCR Primer Design - (reply: 1)
1078. Primers stopped working!? - PCR Primers (reply: 6)
1079. NFK beta primers - (reply: 4)
1080. final PCR product - need clarifications (reply: 1)