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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1051. colony PCR - (reply: 2)
1052. copy number variation - (reply: 3)
1053. How to deal with truncated mRNA or mRNA that has secondary structure - (reply: 2)
1054. Very weird gel - I cannot explain these results (reply: 11)
1055. well position effects (edge and corner) effects in qPCR - using Bio-Rad iCycler iQ (reply: 2)
1056. Cytosolic Housekeeping gene - (reply: 1)
1057. PCR reaction without template gives a product - (reply: 6)
1058. PCR wrong product size - (reply: 6)
1059. PCR very faint product - (reply: 1)
1060. proteinase k inhibition/inactivation - method... (reply: 2)
1061. What does melting temperature indicate and how to predict annealing temperature - (reply: 9)
1062. 4 question for real time PCR - (reply: 5)
1063. RNA quality - (reply: 3)
1064. Superscript III - No second strand synthesis? - (reply: 1)
1065. cDNA convertion to DNA - (reply: 4)
1066. PCR didn't work - suspect the enzyme (reply: 2)
1067. How to check RNA quality - (reply: 5)
1068. Colony PCR Specificity - (reply: 2)
1069. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
1070. Reverse Transcriptase @ Room temperature for 48 hours - (reply: 9)
1071. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
1072. Primer Dilution Problem - D'oh! (reply: 2)
1073. Using PCR to create overhangs - (reply: 3)
1074. Site directed mutation - Changing D->K (reply: 2)
1075. Miner groove binder - urgent, help, what is it? (reply: 4)
1076. Problem with PCR on bacmids - (reply: 1)
1077. Copy number assay using Real time Pcr-SYBR green assay? - (reply: 3)
1078. FAM/HEX, lightcyler 480 - (reply: 1)
1079. Running real time pcr product on gel?.. - (reply: 4)
1080. help with ct of reference gene - (reply: 1)