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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1051. PCR, using gel extracted band as a template - (reply: 4)
1052. Question of nucleic acid extraction - (reply: 1)
1053. Multiplex problems - (reply: 6)
1054. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
1055. Q-PCR and RT-PCR - (reply: 3)
1056. inter-run calibration - (reply: 4)
1057. Delta Delta Ct method for multiple reference genes? - (reply: 1)
1058. FAM and VIC labelling - (reply: 2)
1059. Lost cause?? - I made a silly (reply: 4)
1060. how to use delta-delta Ct method? - when no expression in healthy controls! (reply: 2)
1061. confusing PCR - (reply: 1)
1062. difference between PCR primers and sequencing primers - (reply: 1)
1063. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
1064. making gel electrophoresis - (reply: 1)
1065. housekeeping genes in PCR - (reply: 1)
1066. Question about RealTime PCR Primer Design - (reply: 1)
1067. Primers stopped working!? - PCR Primers (reply: 6)
1068. NFK beta primers - (reply: 4)
1069. final PCR product - need clarifications (reply: 1)
1070. Trying to amplify the 3' end of Prolyl endopeptidse gene from cDNA.. need su - Hi, I'm trying to sequence a gene from the cDNA of an insect (reply: 2)
1071. Can I use Hot Start Taq polimerase for any target? - (reply: 6)
1072. Ct value and interpretation - (reply: 3)
1073. 16S rDNA - Bacteria identification (reply: 1)
1074. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
1075. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
1076. gene expressional analysis - (reply: 1)
1077. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
1078. Troubleshooting help: Why do my amplification curves look like this? - (reply: 8)
1079. why genomic can't be use for pcr? - (reply: 2)
1080. Re-use unamplified PCR product - (reply: 2)