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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
931. Primer design - (reply: 2)
932. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
933. qPCR amplification interference fixed with freezing - (reply: 6)
934. Suggest me a mastermix for conventinal PCR... - (reply: 11)
935. reaction efficiency - (reply: 4)
936. software for quantifing PCR product band - (reply: 1)
937. Thermal cycler - (reply: 2)
938. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
939. qPCR volume - Problem with volume difference and results (reply: 1)
940. RT-PCR - (reply: 9)
941. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
942. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
943. cDNA synthesis - (reply: 6)
944. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
945. Primer seq - (reply: 2)
946. Selection of housekeeping gene in Mycobacterium - (reply: 1)
947. Do I need to pool the base line samples together - (reply: 2)
948. PCR product size - (reply: 6)
949. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
950. Few basic questions about RNA - (reply: 3)
951. oligo dt and random primer - (reply: 3)
952. Ct's of goi higher and lower than ct's hkg - (reply: 1)
953. Effect of collagenase on qpcr results - (reply: 1)
954. RNA Isolation - (reply: 3)
955. mRNA Search for RT-PCR (U to T) - (reply: 2)
956. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
957. PCR triplicates versus one reaction - differences? (reply: 17)
958. cloning of race products - (reply: 1)
959. Single or double-stranded cDNA? - (reply: 2)
960. Comparing SYBR and probe-based Ct values - Is it valid? (reply: 2)