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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
931. Single or double-stranded cDNA? - (reply: 2)
932. Comparing SYBR and probe-based Ct values - Is it valid? (reply: 2)
933. qPCR inhibition - (reply: 1)
934. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
935. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
936. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
937. Primers and self and hetero dimers - (reply: 1)
938. Really weird amplification curves - (reply: 1)
939. Primers for qPCR - (reply: 3)
940. copy number calculation from cDNA - (reply: 1)
941. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
942. RT-PCR primer - (reply: 3)
943. CT value>25 with GAPDH in 50-fold dilution! - (reply: 3)
944. PCR problem - basic PCR for plasmid amplification (reply: 2)
945. can RNA amplify in two-step qPCR? - (reply: 2)
946. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
947. Nested LA PCR - Any success? (reply: 3)
948. Unpredictablity of PCR product - (reply: 5)
949. total cDNA amplification by PCR - (reply: 2)
950. Detection limit of a conventional PCR - (reply: 2)
951. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
952. RNAi showing upregulation via QPCR - Help needed (reply: 3)
953. DNA detection limit of the PCR - Calculating detection limits (reply: 4)
954. cDNA synthesis - (reply: 3)
955. 2 rounds PCR got problem - (reply: 2)
956. PCR help minus strand - Primer design (reply: 1)
957. cDNA contamination, pls help! - (reply: 1)
958. Smear in long distance PCR - (reply: 39)
959. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
960. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)