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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
931. PCR, using gel extracted band as a template - (reply: 4)
932. Question of nucleic acid extraction - (reply: 1)
933. Multiplex problems - (reply: 6)
934. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
935. Q-PCR and RT-PCR - (reply: 3)
936. inter-run calibration - (reply: 4)
937. Delta Delta Ct method for multiple reference genes? - (reply: 1)
938. FAM and VIC labelling - (reply: 2)
939. Lost cause?? - I made a silly (reply: 4)
940. how to use delta-delta Ct method? - when no expression in healthy controls! (reply: 2)
941. confusing PCR - (reply: 1)
942. difference between PCR primers and sequencing primers - (reply: 1)
943. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
944. making gel electrophoresis - (reply: 1)
945. housekeeping genes in PCR - (reply: 1)
946. Question about RealTime PCR Primer Design - (reply: 1)
947. Primers stopped working!? - PCR Primers (reply: 6)
948. NFK beta primers - (reply: 4)
949. final PCR product - need clarifications (reply: 1)
950. Trying to amplify the 3' end of Prolyl endopeptidse gene from cDNA.. need su - Hi, I'm trying to sequence a gene from the cDNA of an insect (reply: 2)
951. Can I use Hot Start Taq polimerase for any target? - (reply: 6)
952. Ct value and interpretation - (reply: 3)
953. 16S rDNA - Bacteria identification (reply: 1)
954. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
955. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
956. gene expressional analysis - (reply: 1)
957. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
958. Troubleshooting help: Why do my amplification curves look like this? - (reply: 8)
959. why genomic can't be use for pcr? - (reply: 2)
960. Re-use unamplified PCR product - (reply: 2)