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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
931. problems amplifying from cDNA - (reply: 4)
932. Real-time PCR about Incorrect setup of the data collection stage - (reply: 1)
933. Primers... - (reply: 1)
934. 18s as endogenous control for Oligo dT RT - Can we use 18s ? (reply: 2)
935. excel and propagation of error, qpcr - (reply: 1)
936. Reverse transcription (cDNA synthesis) curiosity and confusion - Does the amount of RNA have to be doubled if you double everything els (reply: 4)
937. primer with higher melting temperature - (reply: 2)
938. Omitting Points in Standard Curve - (reply: 2)
939. Triage of miRNA targets by qPCR - (reply: 1)
940. Smallest reaction volume with Roche Lightcycler 480 96-well plate format - (reply: 2)
941. qPCR primer design - possible off-target (reply: 1)
942. Primer dimer formation and real-time PCR - (reply: 7)
943. 5' race - (reply: 2)
944. Identify PCR products - (reply: 3)
945. Maximum size of overhangs in PCR - (reply: 6)
946. water-droplets in my cycler !!! - experienced such thing ??? (reply: 9)
947. a second small peak to the right to my pricipal peak!!! - (reply: 1)
948. qPCR virgin, What do I do first? - How to set up experiment (reply: 1)
949. Colony PCR doesn't work anymore - (reply: 5)
950. Question about TM and Annealing temperatures - (reply: 4)
951. RT-PCR t-test, and ANOVA - (reply: 1)
952. PCR basics - I'm new to all things lab and need some help! (reply: 3)
953. the red dye in trizol? - (reply: 2)
954. Is it acceptable that qpcr reaction doesn't reach plateau? - (reply: 3)
955. What are exogeneous and endogeneous controls? - (reply: 4)
956. quantitate wild type and mutant mRNA - (reply: 1)
957. Can very low RNA concentration cause Ct bias..? - (reply: 3)
958. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
959. iScript BioRad cDNA synthesis yield - (reply: 1)
960. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)