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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
931. PCR product size - (reply: 6)
932. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
933. Few basic questions about RNA - (reply: 3)
934. oligo dt and random primer - (reply: 3)
935. Ct's of goi higher and lower than ct's hkg - (reply: 1)
936. Effect of collagenase on qpcr results - (reply: 1)
937. RNA Isolation - (reply: 3)
938. mRNA Search for RT-PCR (U to T) - (reply: 2)
939. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
940. PCR triplicates versus one reaction - differences? (reply: 17)
941. cloning of race products - (reply: 1)
942. Single or double-stranded cDNA? - (reply: 2)
943. Comparing SYBR and probe-based Ct values - Is it valid? (reply: 2)
944. qPCR inhibition - (reply: 1)
945. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
946. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
947. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
948. Primers and self and hetero dimers - (reply: 1)
949. Really weird amplification curves - (reply: 1)
950. Primers for qPCR - (reply: 3)
951. copy number calculation from cDNA - (reply: 1)
952. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
953. RT-PCR primer - (reply: 3)
954. CT value>25 with GAPDH in 50-fold dilution! - (reply: 3)
955. PCR problem - basic PCR for plasmid amplification (reply: 2)
956. can RNA amplify in two-step qPCR? - (reply: 2)
957. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
958. Nested LA PCR - Any success? (reply: 3)
959. Unpredictablity of PCR product - (reply: 5)
960. total cDNA amplification by PCR - (reply: 2)