Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
931. Sybr Green Real Time PCR - Amplification plot - Any problem? (reply: 1)
932. Exon Spanning PCR Primers - (reply: 1)
933. Primer sequences - (reply: 1)
934. Nuclear or Cytoplasmic RNA Purification - (reply: 1)
935. >100% qPCR efficiency - (reply: 2)
936. Why does freezing/thawing DNA samples first improve PCR results? - (reply: 8)
937. PCR Primers - (reply: 2)
938. Cleaning up RT-PCR product before sequencing - (reply: 1)
939. reference gene - (reply: 2)
940. Improving qRT-PCR detection limits - (reply: 2)
941. What to use for Standard Curve - (reply: 2)
942. gPCR - (reply: 1)
943. RT-PCR cDNA synthesis - (reply: 6)
944. problems amplifying from cDNA - (reply: 4)
945. Real-time PCR about Incorrect setup of the data collection stage - (reply: 1)
946. Primers... - (reply: 1)
947. 18s as endogenous control for Oligo dT RT - Can we use 18s ? (reply: 2)
948. excel and propagation of error, qpcr - (reply: 1)
949. Reverse transcription (cDNA synthesis) curiosity and confusion - Does the amount of RNA have to be doubled if you double everything els (reply: 4)
950. primer with higher melting temperature - (reply: 2)
951. Omitting Points in Standard Curve - (reply: 2)
952. Triage of miRNA targets by qPCR - (reply: 1)
953. Smallest reaction volume with Roche Lightcycler 480 96-well plate format - (reply: 2)
954. qPCR primer design - possible off-target (reply: 1)
955. Primer dimer formation and real-time PCR - (reply: 7)
956. 5' race - (reply: 2)
957. Identify PCR products - (reply: 3)
958. Maximum size of overhangs in PCR - (reply: 6)
959. water-droplets in my cycler !!! - experienced such thing ??? (reply: 9)
960. a second small peak to the right to my pricipal peak!!! - (reply: 1)