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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
931. weird QPCR curves - (reply: 2)
932. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
933. Questions regarding RT-PCR optimization - (reply: 2)
934. Problems with semi-quatitative PCR - (reply: 1)
935. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
936. Long-amplicon QPCR of mtDNA - (reply: 5)
937. PCR reaction calculation - (reply: 2)
938. Qiagen rotorgene syber green kit reaction volume - (reply: 1)
939. missing nt at the 5' end - site mutagensis using NEB kit (reply: 1)
940. no increase in fluorescence in my real time PCR - (reply: 3)
941. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
942. Roche second derivative maximum - can be treated according to Pfaffl? (reply: 1)
943. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
944. Pooled RNA from healthy controls - delta-delta Ct method (reply: 1)
945. Probe Dilution Problem = 100uM to 5ng/ul? + Final Volume for FISH - Probe dilution and FISH - Advise on volume for FISH hibridization buff (reply: 2)
946. PCR, using gel extracted band as a template - (reply: 4)
947. Question of nucleic acid extraction - (reply: 1)
948. Multiplex problems - (reply: 6)
949. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
950. Q-PCR and RT-PCR - (reply: 3)
951. inter-run calibration - (reply: 4)
952. Delta Delta Ct method for multiple reference genes? - (reply: 1)
953. FAM and VIC labelling - (reply: 2)
954. Lost cause?? - I made a silly (reply: 4)
955. how to use delta-delta Ct method? - when no expression in healthy controls! (reply: 2)
956. confusing PCR - (reply: 1)
957. difference between PCR primers and sequencing primers - (reply: 1)
958. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
959. making gel electrophoresis - (reply: 1)
960. housekeeping genes in PCR - (reply: 1)