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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
931. Primer sequences - (reply: 1)
932. Nuclear or Cytoplasmic RNA Purification - (reply: 1)
933. >100% qPCR efficiency - (reply: 2)
934. Why does freezing/thawing DNA samples first improve PCR results? - (reply: 8)
935. PCR Primers - (reply: 2)
936. Cleaning up RT-PCR product before sequencing - (reply: 1)
937. reference gene - (reply: 2)
938. Improving qRT-PCR detection limits - (reply: 2)
939. What to use for Standard Curve - (reply: 2)
940. gPCR - (reply: 1)
941. RT-PCR cDNA synthesis - (reply: 6)
942. problems amplifying from cDNA - (reply: 4)
943. Real-time PCR about Incorrect setup of the data collection stage - (reply: 1)
944. Primers... - (reply: 1)
945. 18s as endogenous control for Oligo dT RT - Can we use 18s ? (reply: 2)
946. excel and propagation of error, qpcr - (reply: 1)
947. Reverse transcription (cDNA synthesis) curiosity and confusion - Does the amount of RNA have to be doubled if you double everything els (reply: 4)
948. primer with higher melting temperature - (reply: 2)
949. Omitting Points in Standard Curve - (reply: 2)
950. Triage of miRNA targets by qPCR - (reply: 1)
951. Smallest reaction volume with Roche Lightcycler 480 96-well plate format - (reply: 2)
952. qPCR primer design - possible off-target (reply: 1)
953. Primer dimer formation and real-time PCR - (reply: 7)
954. 5' race - (reply: 2)
955. Identify PCR products - (reply: 3)
956. Maximum size of overhangs in PCR - (reply: 6)
957. water-droplets in my cycler !!! - experienced such thing ??? (reply: 9)
958. a second small peak to the right to my pricipal peak!!! - (reply: 1)
959. qPCR virgin, What do I do first? - How to set up experiment (reply: 1)
960. Colony PCR doesn't work anymore - (reply: 5)