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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
931. 2 rounds PCR got problem - (reply: 2)
932. PCR help minus strand - Primer design (reply: 1)
933. cDNA contamination, pls help! - (reply: 1)
934. Smear in long distance PCR - (reply: 39)
935. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
936. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
937. can contaminating gDNA explain this? - (reply: 1)
938. TOUCH DOWN PCR - (reply: 2)
939. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
940. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
941. PCR stopped working - After changing buffer (reply: 5)
942. how to determine the amount of cDNA for qPCR - (reply: 5)
943. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)
944. Standardization of HRM for methylation analysis - (reply: 4)
945. PCR protocol questions! - (reply: 2)
946. standard curve for qPCR - (reply: 3)
947. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
948. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
949. DNA amount calculation for PCR - (reply: 14)
950. ChIP primer design - (reply: 1)
951. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
952. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
953. weird QPCR curves - (reply: 2)
954. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
955. Questions regarding RT-PCR optimization - (reply: 2)
956. Problems with semi-quatitative PCR - (reply: 1)
957. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
958. Long-amplicon QPCR of mtDNA - (reply: 5)
959. PCR reaction calculation - (reply: 2)
960. Qiagen rotorgene syber green kit reaction volume - (reply: 1)