Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
751. HaCaT reference gene - (reply: 1)
752. Need help for PCR for AT rich gene - (reply: 3)
753. Amplify fragment in plasmid? - (reply: 4)
754. Relative quantification of different genes in single sample - same threshold val - (reply: 1)
755. Primer validation and PCR efficiency - (reply: 4)
756. How to calculate the price of PCR test per sample? - (reply: 6)
757. dilution help - (reply: 6)
758. rityProbe Integrity in Mastermix during loading 96 well plate - (reply: 2)
759. cloning with nested pcr - (reply: 3)
760. how to find 16srRNA sequences? - (reply: 3)
761. RNA extraction from a 1mm brain tissue punch - uncertain about homogenisation - (reply: 2)
762. What is the maxium amount of glycerol can be added in a PCR reaction? - (reply: 3)
763. PCR is amplifying non-specific fragment - (reply: 6)
764. Anybody know about temperature sensitivity of the AB TaqMan universal Mastermix - (reply: 4)
765. Measuring of cDNA bands density - (reply: 3)
766. real time RT-PCR problems - (reply: 2)
767. Maximum Annealing-Temperature for Real-Time? - (reply: 3)
768. Real-Time efficiencies and the pfaffl method - (reply: 1)
769. should I design primers spanning both intron and exon - (reply: 2)
770. RT-PCR - (reply: 1)
771. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
772. PCR with a very long and a short primer - (reply: 5)
773. qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
774. How to find that my RNA is not contaminated with gDNA, before running Real-Time - (reply: 5)
775. Dilution of 18s. How much? - (reply: 1)
776. Multiplex PCR does not work - (reply: 3)
777. Alternative to Sybr-green? - (reply: 12)
778. Multiple internal controls in using qPCR to measure gene duplication - (reply: 1)
779. ct value of neg - (reply: 1)
780. Help: Linear Amplification Using Hydrolysis Probes - (reply: 3)