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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
751. Sybr green RT-qPCR primer - (reply: 1)
752. Sybr green RT-qPCR primer - (reply: 1)
753. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
754. Oligo Question - (reply: 1)
755. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
756. real time rt PCR - bloodyurine - inhibition - (reply: 1)
757. No Amplification in PCR - (reply: 4)
758. DNase treatment after RNA extraction using RNeasy kit of qiagen - DNaes treatment after RNA extraction (reply: 2)
759. Real time PCR and percentage loss - (reply: 1)
760. Problem with smear in PCR with certain templates - (reply: 4)
761. running multiple qPCR with common housekeeping control - (reply: 3)
762. Problems with PCR in general - (reply: 1)
763. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 2)
764. Control inconsistence in Q-PCR - (reply: 1)
765. how to overcome primer contamination - need protocol (reply: 11)
766. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
767. sequencing problem - (reply: 1)
768. High Resolution Melting (HRM) Problem - (reply: 4)
769. Consistancy in qPCR data - (reply: 5)
770. RT-PCR primer design - Primer design and evaluation (reply: 7)
771. qPCR with low expressing genes - (reply: 1)
772. Product size? - (reply: 7)
773. Contamination issues - (reply: 5)
774. PCRing small region on genomic DNA - (reply: 3)
775. Confirm RACE product - (reply: 3)
776. Reference genes. - (reply: 1)
777. Two peaks generated by one NTC on melting curve. - (reply: 2)
778. Splice varient quantification in RT real time PCR? - (reply: 1)
779. Help with delta delta ct analysis - (reply: 1)
780. Calculating geometric mean for real-time PCR - (reply: 5)