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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
751. Adding loading/tracking dye to PCR mix? - (reply: 10)
752. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
753. internal control in RT-PCR - inquiry (reply: 2)
754. Melting Temp of Standards Changed? - (reply: 3)
755. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
756. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
757. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
758. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
759. Gene on negative strand - two eductional questions. - (reply: 3)
760. Newbie- choose DNA template - (reply: 1)
761. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
762. Total RNA estimation - (reply: 2)
763. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
764. pcr contamination - (reply: 4)
765. Large Non-specfic bands in PCR - (reply: 3)
766. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
767. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
768. Primer dilution --> problem.. - (reply: 16)
769. no bands after pcr - even after we have change most of the reagents (reply: 2)
770. High resolution melting to gene scanning - (reply: 1)
771. Help! Smear in Real-Time PCR Product - (reply: 1)
772. Is one reference gene ever appropriate? - (reply: 3)
773. Sybr green RT-qPCR primer - (reply: 1)
774. Sybr green RT-qPCR primer - (reply: 1)
775. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
776. Oligo Question - (reply: 1)
777. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
778. real time rt PCR - bloodyurine - inhibition - (reply: 1)
779. No Amplification in PCR - (reply: 4)
780. DNase treatment after RNA extraction using RNeasy kit of qiagen - DNaes treatment after RNA extraction (reply: 2)