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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
751. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
752. pcr contamination - (reply: 4)
753. Large Non-specfic bands in PCR - (reply: 3)
754. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
755. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
756. Primer dilution --> problem.. - (reply: 16)
757. no bands after pcr - even after we have change most of the reagents (reply: 2)
758. High resolution melting to gene scanning - (reply: 1)
759. Help! Smear in Real-Time PCR Product - (reply: 1)
760. Is one reference gene ever appropriate? - (reply: 3)
761. Sybr green RT-qPCR primer - (reply: 1)
762. Sybr green RT-qPCR primer - (reply: 1)
763. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
764. Oligo Question - (reply: 1)
765. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
766. real time rt PCR - bloodyurine - inhibition - (reply: 1)
767. No Amplification in PCR - (reply: 4)
768. DNase treatment after RNA extraction using RNeasy kit of qiagen - DNaes treatment after RNA extraction (reply: 2)
769. Real time PCR and percentage loss - (reply: 1)
770. Problem with smear in PCR with certain templates - (reply: 4)
771. running multiple qPCR with common housekeeping control - (reply: 3)
772. Problems with PCR in general - (reply: 1)
773. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 2)
774. Control inconsistence in Q-PCR - (reply: 1)
775. how to overcome primer contamination - need protocol (reply: 11)
776. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
777. sequencing problem - (reply: 1)
778. High Resolution Melting (HRM) Problem - (reply: 4)
779. Consistancy in qPCR data - (reply: 5)
780. RT-PCR primer design - Primer design and evaluation (reply: 7)