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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
751. Error bars for qPCR; technical vs. biological replicate - (reply: 2)
752. HaCaT reference gene - (reply: 1)
753. Need help for PCR for AT rich gene - (reply: 3)
754. Amplify fragment in plasmid? - (reply: 4)
755. Relative quantification of different genes in single sample - same threshold val - (reply: 1)
756. Primer validation and PCR efficiency - (reply: 4)
757. How to calculate the price of PCR test per sample? - (reply: 6)
758. dilution help - (reply: 6)
759. rityProbe Integrity in Mastermix during loading 96 well plate - (reply: 2)
760. cloning with nested pcr - (reply: 3)
761. how to find 16srRNA sequences? - (reply: 3)
762. RNA extraction from a 1mm brain tissue punch - uncertain about homogenisation - (reply: 2)
763. What is the maxium amount of glycerol can be added in a PCR reaction? - (reply: 3)
764. PCR is amplifying non-specific fragment - (reply: 6)
765. Anybody know about temperature sensitivity of the AB TaqMan universal Mastermix - (reply: 4)
766. Measuring of cDNA bands density - (reply: 3)
767. real time RT-PCR problems - (reply: 2)
768. Maximum Annealing-Temperature for Real-Time? - (reply: 3)
769. Real-Time efficiencies and the pfaffl method - (reply: 1)
770. should I design primers spanning both intron and exon - (reply: 2)
771. RT-PCR - (reply: 1)
772. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
773. PCR with a very long and a short primer - (reply: 5)
774. qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
775. How to find that my RNA is not contaminated with gDNA, before running Real-Time - (reply: 5)
776. Dilution of 18s. How much? - (reply: 1)
777. Multiplex PCR does not work - (reply: 3)
778. Alternative to Sybr-green? - (reply: 12)
779. Multiple internal controls in using qPCR to measure gene duplication - (reply: 1)
780. ct value of neg - (reply: 1)