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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
751. Relative quantification of different genes in single sample - same threshold val - (reply: 1)
752. Primer validation and PCR efficiency - (reply: 4)
753. How to calculate the price of PCR test per sample? - (reply: 6)
754. dilution help - (reply: 6)
755. rityProbe Integrity in Mastermix during loading 96 well plate - (reply: 2)
756. cloning with nested pcr - (reply: 3)
757. how to find 16srRNA sequences? - (reply: 3)
758. RNA extraction from a 1mm brain tissue punch - uncertain about homogenisation - (reply: 2)
759. What is the maxium amount of glycerol can be added in a PCR reaction? - (reply: 3)
760. PCR is amplifying non-specific fragment - (reply: 6)
761. Anybody know about temperature sensitivity of the AB TaqMan universal Mastermix - (reply: 4)
762. Measuring of cDNA bands density - (reply: 3)
763. real time RT-PCR problems - (reply: 2)
764. Maximum Annealing-Temperature for Real-Time? - (reply: 3)
765. Real-Time efficiencies and the pfaffl method - (reply: 1)
766. should I design primers spanning both intron and exon - (reply: 2)
767. RT-PCR - (reply: 1)
768. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
769. PCR with a very long and a short primer - (reply: 5)
770. qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
771. How to find that my RNA is not contaminated with gDNA, before running Real-Time - (reply: 5)
772. Dilution of 18s. How much? - (reply: 1)
773. Multiplex PCR does not work - (reply: 3)
774. Alternative to Sybr-green? - (reply: 12)
775. Multiple internal controls in using qPCR to measure gene duplication - (reply: 1)
776. ct value of neg - (reply: 1)
777. Help: Linear Amplification Using Hydrolysis Probes - (reply: 3)
778. Standard curve - (reply: 3)
779. Discuss about COLD-PCR - (reply: 3)
780. Comparison between two primers pairs? Is it possible? - (reply: 3)