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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
841. what's the function of ROX reference dye? - (reply: 2)
842. Gene Expression Issues - (reply: 3)
843. clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
844. No gene amplification, 18s amplification is fine - (reply: 1)
845. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
846. What can I do to increase the banding signal? - (reply: 6)
847. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
848. Tomato Actin PCR+Images included - (reply: 2)
849. single primer PCR - (reply: 5)
850. Weird qPCR curves - (reply: 4)
851. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
852. LacZ PCR Problems - (reply: 1)
853. Primer concentration - (reply: 4)
854. nested PCR with low target DNA? - (reply: 2)
855. RT-PCR calibrator - (reply: 2)
856. problem in pcr - (reply: 2)
857. Can someone recommend me a virtual PCR software? - (reply: 6)
858. negative values after GOI - reference gene ?? - (reply: 4)
859. RNA degradation during electrophoresis?? - (reply: 2)
860. Primer 3' mismatch - Strange experience (reply: 9)
861. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
862. Adding loading/tracking dye to PCR mix? - (reply: 10)
863. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
864. internal control in RT-PCR - inquiry (reply: 2)
865. Melting Temp of Standards Changed? - (reply: 3)
866. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
867. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
868. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
869. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
870. Gene on negative strand - two eductional questions. - (reply: 3)