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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
841. Primers... - (reply: 1)
842. 18s as endogenous control for Oligo dT RT - Can we use 18s ? (reply: 2)
843. excel and propagation of error, qpcr - (reply: 1)
844. Reverse transcription (cDNA synthesis) curiosity and confusion - Does the amount of RNA have to be doubled if you double everything els (reply: 4)
845. primer with higher melting temperature - (reply: 2)
846. Omitting Points in Standard Curve - (reply: 2)
847. Triage of miRNA targets by qPCR - (reply: 1)
848. Smallest reaction volume with Roche Lightcycler 480 96-well plate format - (reply: 2)
849. qPCR primer design - possible off-target (reply: 1)
850. Primer dimer formation and real-time PCR - (reply: 7)
851. 5' race - (reply: 2)
852. Identify PCR products - (reply: 3)
853. Maximum size of overhangs in PCR - (reply: 6)
854. water-droplets in my cycler !!! - experienced such thing ??? (reply: 9)
855. a second small peak to the right to my pricipal peak!!! - (reply: 1)
856. qPCR virgin, What do I do first? - How to set up experiment (reply: 1)
857. Colony PCR doesn't work anymore - (reply: 5)
858. Question about TM and Annealing temperatures - (reply: 4)
859. RT-PCR t-test, and ANOVA - (reply: 1)
860. PCR basics - I'm new to all things lab and need some help! (reply: 3)
861. the red dye in trizol? - (reply: 2)
862. Is it acceptable that qpcr reaction doesn't reach plateau? - (reply: 3)
863. What are exogeneous and endogeneous controls? - (reply: 4)
864. quantitate wild type and mutant mRNA - (reply: 1)
865. Can very low RNA concentration cause Ct bias..? - (reply: 3)
866. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
867. iScript BioRad cDNA synthesis yield - (reply: 1)
868. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
869. intron spanning primers for non model organisms - (reply: 2)
870. Using DNA as a reference in qRT-PCR - (reply: 1)