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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
841. quantitate wild type and mutant mRNA - (reply: 1)
842. Can very low RNA concentration cause Ct bias..? - (reply: 3)
843. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
844. iScript BioRad cDNA synthesis yield - (reply: 1)
845. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
846. intron spanning primers for non model organisms - (reply: 2)
847. Using DNA as a reference in qRT-PCR - (reply: 1)
848. minimum replicate for qpcr? - (reply: 15)
849. Primer design - (reply: 2)
850. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
851. qPCR amplification interference fixed with freezing - (reply: 6)
852. Suggest me a mastermix for conventinal PCR... - (reply: 11)
853. reaction efficiency - (reply: 4)
854. software for quantifing PCR product band - (reply: 1)
855. Thermal cycler - (reply: 2)
856. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
857. qPCR volume - Problem with volume difference and results (reply: 1)
858. RT-PCR - (reply: 9)
859. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
860. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
861. cDNA synthesis - (reply: 6)
862. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
863. Primer seq - (reply: 2)
864. Selection of housekeeping gene in Mycobacterium - (reply: 1)
865. Do I need to pool the base line samples together - (reply: 2)
866. PCR product size - (reply: 6)
867. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
868. Few basic questions about RNA - (reply: 3)
869. oligo dt and random primer - (reply: 3)
870. Ct's of goi higher and lower than ct's hkg - (reply: 1)