Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
841. CT value too small - (reply: 1)
842. Need help amplifying repeating sequence. - (reply: 5)
843. RNA isolation from frozen cells - (reply: 2)
844. Another primer dimers problem - (reply: 23)
845. using PCR product as standard template - (reply: 3)
846. Amplification in water control but not in samples - (reply: 3)
847. PCR products form with DNA, but not with c-DNA - (reply: 2)
848. question about pcr amplification efficiency? - (reply: 1)
849. Which Cycler to buy? - Can you recommend any of these cyclers? (reply: 2)
850. a question about the annealing temperature - (reply: 6)
851. what's the function of ROX reference dye? - (reply: 2)
852. Gene Expression Issues - (reply: 3)
853. clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
854. No gene amplification, 18s amplification is fine - (reply: 1)
855. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
856. What can I do to increase the banding signal? - (reply: 6)
857. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
858. Tomato Actin PCR+Images included - (reply: 2)
859. single primer PCR - (reply: 5)
860. Weird qPCR curves - (reply: 4)
861. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
862. LacZ PCR Problems - (reply: 1)
863. Primer concentration - (reply: 4)
864. nested PCR with low target DNA? - (reply: 2)
865. RT-PCR calibrator - (reply: 2)
866. problem in pcr - (reply: 2)
867. Can someone recommend me a virtual PCR software? - (reply: 6)
868. negative values after GOI - reference gene ?? - (reply: 4)
869. RNA degradation during electrophoresis?? - (reply: 2)
870. Primer 3' mismatch - Strange experience (reply: 9)