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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
841. software for quantifing PCR product band - (reply: 1)
842. Thermal cycler - (reply: 2)
843. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
844. qPCR volume - Problem with volume difference and results (reply: 1)
845. RT-PCR - (reply: 9)
846. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
847. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
848. cDNA synthesis - (reply: 6)
849. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
850. Primer seq - (reply: 2)
851. Selection of housekeeping gene in Mycobacterium - (reply: 1)
852. Do I need to pool the base line samples together - (reply: 2)
853. PCR product size - (reply: 6)
854. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
855. Few basic questions about RNA - (reply: 3)
856. oligo dt and random primer - (reply: 3)
857. Ct's of goi higher and lower than ct's hkg - (reply: 1)
858. Effect of collagenase on qpcr results - (reply: 1)
859. RNA Isolation - (reply: 3)
860. mRNA Search for RT-PCR (U to T) - (reply: 2)
861. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
862. PCR triplicates versus one reaction - differences? (reply: 17)
863. cloning of race products - (reply: 1)
864. Single or double-stranded cDNA? - (reply: 2)
865. Comparing SYBR and probe-based Ct values - Is it valid? (reply: 2)
866. qPCR inhibition - (reply: 1)
867. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
868. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
869. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
870. Primers and self and hetero dimers - (reply: 1)