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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
841. RNA isolation from frozen cells - (reply: 2)
842. Another primer dimers problem - (reply: 23)
843. using PCR product as standard template - (reply: 3)
844. Amplification in water control but not in samples - (reply: 3)
845. PCR products form with DNA, but not with c-DNA - (reply: 2)
846. question about pcr amplification efficiency? - (reply: 1)
847. Which Cycler to buy? - Can you recommend any of these cyclers? (reply: 2)
848. a question about the annealing temperature - (reply: 6)
849. what's the function of ROX reference dye? - (reply: 2)
850. Gene Expression Issues - (reply: 3)
851. clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
852. No gene amplification, 18s amplification is fine - (reply: 1)
853. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
854. What can I do to increase the banding signal? - (reply: 6)
855. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
856. Tomato Actin PCR+Images included - (reply: 2)
857. single primer PCR - (reply: 5)
858. Weird qPCR curves - (reply: 4)
859. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
860. LacZ PCR Problems - (reply: 1)
861. Primer concentration - (reply: 4)
862. nested PCR with low target DNA? - (reply: 2)
863. RT-PCR calibrator - (reply: 2)
864. problem in pcr - (reply: 2)
865. Can someone recommend me a virtual PCR software? - (reply: 6)
866. negative values after GOI - reference gene ?? - (reply: 4)
867. RNA degradation during electrophoresis?? - (reply: 2)
868. Primer 3' mismatch - Strange experience (reply: 9)
869. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
870. Adding loading/tracking dye to PCR mix? - (reply: 10)