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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
841. qPCR primer design - possible off-target (reply: 1)
842. Primer dimer formation and real-time PCR - (reply: 7)
843. 5' race - (reply: 2)
844. Identify PCR products - (reply: 3)
845. Maximum size of overhangs in PCR - (reply: 6)
846. water-droplets in my cycler !!! - experienced such thing ??? (reply: 9)
847. a second small peak to the right to my pricipal peak!!! - (reply: 1)
848. qPCR virgin, What do I do first? - How to set up experiment (reply: 1)
849. Colony PCR doesn't work anymore - (reply: 5)
850. Question about TM and Annealing temperatures - (reply: 4)
851. RT-PCR t-test, and ANOVA - (reply: 1)
852. PCR basics - I'm new to all things lab and need some help! (reply: 3)
853. the red dye in trizol? - (reply: 2)
854. Is it acceptable that qpcr reaction doesn't reach plateau? - (reply: 3)
855. What are exogeneous and endogeneous controls? - (reply: 4)
856. quantitate wild type and mutant mRNA - (reply: 1)
857. Can very low RNA concentration cause Ct bias..? - (reply: 3)
858. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
859. iScript BioRad cDNA synthesis yield - (reply: 1)
860. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
861. intron spanning primers for non model organisms - (reply: 2)
862. Using DNA as a reference in qRT-PCR - (reply: 1)
863. minimum replicate for qpcr? - (reply: 15)
864. Primer design - (reply: 2)
865. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
866. qPCR amplification interference fixed with freezing - (reply: 6)
867. Suggest me a mastermix for conventinal PCR... - (reply: 11)
868. reaction efficiency - (reply: 4)
869. software for quantifing PCR product band - (reply: 1)
870. Thermal cycler - (reply: 2)