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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
841. How do I insert a DNA sequence into a multiple cloning site and add restriction - (reply: 1)
842. efficiency calculation from non-10 fold dilutions - (reply: 1)
843. whole plasmid pcr? - (reply: 6)
844. Proper storage of isolated white blood cell prior to RNA isolation - (reply: 3)
845. Genomic pseudogenes?!? - (reply: 6)
846. Strange amplification plot - (reply: 4)
847. Conventional PCR of different dilutions - (reply: 1)
848. primer resuspension - (reply: 2)
849. Principles of overlap PCR - (reply: 7)
850. Help designing primers with restriction sites - (reply: 3)
851. DNA sequence data came back with lots of wrong bases - (reply: 5)
852. Why primers shouldn't have Tm difference greater than 5C? - (reply: 5)
853. Abnormal amplification curve - (reply: 1)
854. Problem with Semiquantitative PCR - (reply: 4)
855. false positive/cross reaction with TaqMan - (reply: 1)
856. Problem w/ Genotyping Cre Only - (reply: 3)
857. ROX reference dye for quantitative RT-PCR - (reply: 1)
858. PCR problem - (reply: 9)
859. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
860. why PCR product smeared? - (reply: 2)
861. Tm calculation for primers with RE sites and overhangs - (reply: 4)
862. silincing box, no amplification - (reply: 3)
863. Comparative semiquantitative RT-PCR - (reply: 2)
864. Bands in my PCR Controls - (reply: 3)
865. RTPCR - housekeeping genes: can I use them more intelligently? - (reply: 4)
866. real time qRT-PCR and low expression gene - (reply: 2)
867. In a pcr why doesnt the DNA reneal during annealing process ? - (reply: 2)
868. check points while preparing for sequencing - (reply: 10)
869. Disagreement between triplicate results in Q -PCR - (reply: 5)
870. 40 cT values any idea how to lower it? - (reply: 1)