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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
841. Melting Temp of Standards Changed? - (reply: 3)
842. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
843. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
844. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
845. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
846. Gene on negative strand - two eductional questions. - (reply: 3)
847. Newbie- choose DNA template - (reply: 1)
848. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
849. Total RNA estimation - (reply: 2)
850. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
851. pcr contamination - (reply: 4)
852. Large Non-specfic bands in PCR - (reply: 3)
853. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
854. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
855. Primer dilution --> problem.. - (reply: 16)
856. no bands after pcr - even after we have change most of the reagents (reply: 2)
857. High resolution melting to gene scanning - (reply: 1)
858. Help! Smear in Real-Time PCR Product - (reply: 1)
859. Is one reference gene ever appropriate? - (reply: 3)
860. Sybr green RT-qPCR primer - (reply: 1)
861. Sybr green RT-qPCR primer - (reply: 1)
862. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
863. Oligo Question - (reply: 1)
864. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
865. real time rt PCR - bloodyurine - inhibition - (reply: 1)
866. No Amplification in PCR - (reply: 4)
867. DNase treatment after RNA extraction using RNeasy kit of qiagen - DNaes treatment after RNA extraction (reply: 2)
868. Real time PCR and percentage loss - (reply: 1)
869. Problem with smear in PCR with certain templates - (reply: 4)
870. running multiple qPCR with common housekeeping control - (reply: 3)