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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
841. Few basic questions about RNA - (reply: 3)
842. oligo dt and random primer - (reply: 3)
843. Ct's of goi higher and lower than ct's hkg - (reply: 1)
844. Effect of collagenase on qpcr results - (reply: 1)
845. RNA Isolation - (reply: 3)
846. mRNA Search for RT-PCR (U to T) - (reply: 2)
847. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
848. PCR triplicates versus one reaction - differences? (reply: 17)
849. cloning of race products - (reply: 1)
850. Single or double-stranded cDNA? - (reply: 2)
851. Comparing SYBR and probe-based Ct values - Is it valid? (reply: 2)
852. qPCR inhibition - (reply: 1)
853. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
854. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
855. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
856. Primers and self and hetero dimers - (reply: 1)
857. Really weird amplification curves - (reply: 1)
858. Primers for qPCR - (reply: 3)
859. copy number calculation from cDNA - (reply: 1)
860. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
861. RT-PCR primer - (reply: 3)
862. CT value>25 with GAPDH in 50-fold dilution! - (reply: 3)
863. PCR problem - basic PCR for plasmid amplification (reply: 2)
864. can RNA amplify in two-step qPCR? - (reply: 2)
865. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
866. Nested LA PCR - Any success? (reply: 3)
867. Unpredictablity of PCR product - (reply: 5)
868. total cDNA amplification by PCR - (reply: 2)
869. Detection limit of a conventional PCR - (reply: 2)
870. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)