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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
991. Effect of collagenase on qpcr results - (reply: 1)
992. RNA Isolation - (reply: 3)
993. mRNA Search for RT-PCR (U to T) - (reply: 2)
994. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
995. PCR triplicates versus one reaction - differences? (reply: 17)
996. cloning of race products - (reply: 1)
997. Single or double-stranded cDNA? - (reply: 2)
998. Comparing SYBR and probe-based Ct values - Is it valid? (reply: 2)
999. qPCR inhibition - (reply: 1)
1000. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
1001. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
1002. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
1003. Primers and self and hetero dimers - (reply: 1)
1004. Really weird amplification curves - (reply: 1)
1005. Primers for qPCR - (reply: 3)
1006. copy number calculation from cDNA - (reply: 1)
1007. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
1008. RT-PCR primer - (reply: 3)
1009. CT value>25 with GAPDH in 50-fold dilution! - (reply: 3)
1010. PCR problem - basic PCR for plasmid amplification (reply: 2)
1011. can RNA amplify in two-step qPCR? - (reply: 2)
1012. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
1013. Nested LA PCR - Any success? (reply: 3)
1014. Unpredictablity of PCR product - (reply: 5)
1015. total cDNA amplification by PCR - (reply: 2)
1016. Detection limit of a conventional PCR - (reply: 2)
1017. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
1018. RNAi showing upregulation via QPCR - Help needed (reply: 3)
1019. DNA detection limit of the PCR - Calculating detection limits (reply: 4)
1020. cDNA synthesis - (reply: 3)