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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
991. Primer seq - (reply: 2)
992. Selection of housekeeping gene in Mycobacterium - (reply: 1)
993. Do I need to pool the base line samples together - (reply: 2)
994. PCR product size - (reply: 6)
995. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
996. Few basic questions about RNA - (reply: 3)
997. oligo dt and random primer - (reply: 3)
998. Ct's of goi higher and lower than ct's hkg - (reply: 1)
999. Effect of collagenase on qpcr results - (reply: 1)
1000. RNA Isolation - (reply: 3)
1001. mRNA Search for RT-PCR (U to T) - (reply: 2)
1002. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
1003. PCR triplicates versus one reaction - differences? (reply: 17)
1004. cloning of race products - (reply: 1)
1005. Single or double-stranded cDNA? - (reply: 2)
1006. Comparing SYBR and probe-based Ct values - Is it valid? (reply: 2)
1007. qPCR inhibition - (reply: 1)
1008. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
1009. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
1010. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
1011. Primers and self and hetero dimers - (reply: 1)
1012. Really weird amplification curves - (reply: 1)
1013. Primers for qPCR - (reply: 3)
1014. copy number calculation from cDNA - (reply: 1)
1015. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
1016. RT-PCR primer - (reply: 3)
1017. CT value>25 with GAPDH in 50-fold dilution! - (reply: 3)
1018. PCR problem - basic PCR for plasmid amplification (reply: 2)
1019. can RNA amplify in two-step qPCR? - (reply: 2)
1020. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)