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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
991. Do I need to pool the base line samples together - (reply: 2)
992. PCR product size - (reply: 6)
993. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
994. Few basic questions about RNA - (reply: 3)
995. oligo dt and random primer - (reply: 3)
996. Ct's of goi higher and lower than ct's hkg - (reply: 1)
997. Effect of collagenase on qpcr results - (reply: 1)
998. RNA Isolation - (reply: 3)
999. mRNA Search for RT-PCR (U to T) - (reply: 2)
1000. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
1001. PCR triplicates versus one reaction - differences? (reply: 17)
1002. cloning of race products - (reply: 1)
1003. Single or double-stranded cDNA? - (reply: 2)
1004. Comparing SYBR and probe-based Ct values - Is it valid? (reply: 2)
1005. qPCR inhibition - (reply: 1)
1006. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
1007. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
1008. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
1009. Primers and self and hetero dimers - (reply: 1)
1010. Really weird amplification curves - (reply: 1)
1011. Primers for qPCR - (reply: 3)
1012. copy number calculation from cDNA - (reply: 1)
1013. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
1014. RT-PCR primer - (reply: 3)
1015. CT value>25 with GAPDH in 50-fold dilution! - (reply: 3)
1016. PCR problem - basic PCR for plasmid amplification (reply: 2)
1017. can RNA amplify in two-step qPCR? - (reply: 2)
1018. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
1019. Nested LA PCR - Any success? (reply: 3)
1020. Unpredictablity of PCR product - (reply: 5)