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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
991. Primer dimer formation and real-time PCR - (reply: 7)
992. 5' race - (reply: 2)
993. Identify PCR products - (reply: 3)
994. Maximum size of overhangs in PCR - (reply: 6)
995. water-droplets in my cycler !!! - experienced such thing ??? (reply: 9)
996. a second small peak to the right to my pricipal peak!!! - (reply: 1)
997. qPCR virgin, What do I do first? - How to set up experiment (reply: 1)
998. Colony PCR doesn't work anymore - (reply: 5)
999. Question about TM and Annealing temperatures - (reply: 4)
1000. RT-PCR t-test, and ANOVA - (reply: 1)
1001. PCR basics - I'm new to all things lab and need some help! (reply: 3)
1002. the red dye in trizol? - (reply: 2)
1003. Is it acceptable that qpcr reaction doesn't reach plateau? - (reply: 3)
1004. What are exogeneous and endogeneous controls? - (reply: 4)
1005. quantitate wild type and mutant mRNA - (reply: 1)
1006. Can very low RNA concentration cause Ct bias..? - (reply: 3)
1007. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
1008. iScript BioRad cDNA synthesis yield - (reply: 1)
1009. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
1010. intron spanning primers for non model organisms - (reply: 2)
1011. Using DNA as a reference in qRT-PCR - (reply: 1)
1012. minimum replicate for qpcr? - (reply: 15)
1013. Primer design - (reply: 2)
1014. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
1015. qPCR amplification interference fixed with freezing - (reply: 6)
1016. Suggest me a mastermix for conventinal PCR... - (reply: 11)
1017. reaction efficiency - (reply: 4)
1018. software for quantifing PCR product band - (reply: 1)
1019. Thermal cycler - (reply: 2)
1020. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)