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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
991. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
992. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
993. PCR stopped working - After changing buffer (reply: 5)
994. how to determine the amount of cDNA for qPCR - (reply: 5)
995. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)
996. Standardization of HRM for methylation analysis - (reply: 4)
997. PCR protocol questions! - (reply: 2)
998. standard curve for qPCR - (reply: 3)
999. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
1000. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
1001. DNA amount calculation for PCR - (reply: 14)
1002. ChIP primer design - (reply: 1)
1003. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
1004. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
1005. weird QPCR curves - (reply: 2)
1006. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
1007. Questions regarding RT-PCR optimization - (reply: 2)
1008. Problems with semi-quatitative PCR - (reply: 1)
1009. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
1010. Long-amplicon QPCR of mtDNA - (reply: 5)
1011. PCR reaction calculation - (reply: 2)
1012. Qiagen rotorgene syber green kit reaction volume - (reply: 1)
1013. missing nt at the 5' end - site mutagensis using NEB kit (reply: 1)
1014. no increase in fluorescence in my real time PCR - (reply: 3)
1015. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
1016. Roche second derivative maximum - can be treated according to Pfaffl? (reply: 1)
1017. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
1018. Pooled RNA from healthy controls - delta-delta Ct method (reply: 1)
1019. Probe Dilution Problem = 100uM to 5ng/ul? + Final Volume for FISH - Probe dilution and FISH - Advise on volume for FISH hibridization buff (reply: 2)
1020. PCR, using gel extracted band as a template - (reply: 4)