Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
991. RT-PCR - (reply: 9)
992. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
993. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
994. cDNA synthesis - (reply: 6)
995. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
996. Primer seq - (reply: 2)
997. Selection of housekeeping gene in Mycobacterium - (reply: 1)
998. Do I need to pool the base line samples together - (reply: 2)
999. PCR product size - (reply: 6)
1000. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
1001. Few basic questions about RNA - (reply: 3)
1002. oligo dt and random primer - (reply: 3)
1003. Ct's of goi higher and lower than ct's hkg - (reply: 1)
1004. Effect of collagenase on qpcr results - (reply: 1)
1005. RNA Isolation - (reply: 3)
1006. mRNA Search for RT-PCR (U to T) - (reply: 2)
1007. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
1008. PCR triplicates versus one reaction - differences? (reply: 17)
1009. cloning of race products - (reply: 1)
1010. Single or double-stranded cDNA? - (reply: 2)
1011. Comparing SYBR and probe-based Ct values - Is it valid? (reply: 2)
1012. qPCR inhibition - (reply: 1)
1013. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
1014. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
1015. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
1016. Primers and self and hetero dimers - (reply: 1)
1017. Really weird amplification curves - (reply: 1)
1018. Primers for qPCR - (reply: 3)
1019. copy number calculation from cDNA - (reply: 1)
1020. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)