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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
991. reaction efficiency - (reply: 4)
992. software for quantifing PCR product band - (reply: 1)
993. Thermal cycler - (reply: 2)
994. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
995. qPCR volume - Problem with volume difference and results (reply: 1)
996. RT-PCR - (reply: 9)
997. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
998. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
999. cDNA synthesis - (reply: 6)
1000. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
1001. Primer seq - (reply: 2)
1002. Selection of housekeeping gene in Mycobacterium - (reply: 1)
1003. Do I need to pool the base line samples together - (reply: 2)
1004. PCR product size - (reply: 6)
1005. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
1006. Few basic questions about RNA - (reply: 3)
1007. oligo dt and random primer - (reply: 3)
1008. Ct's of goi higher and lower than ct's hkg - (reply: 1)
1009. Effect of collagenase on qpcr results - (reply: 1)
1010. RNA Isolation - (reply: 3)
1011. mRNA Search for RT-PCR (U to T) - (reply: 2)
1012. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
1013. PCR triplicates versus one reaction - differences? (reply: 17)
1014. cloning of race products - (reply: 1)
1015. Single or double-stranded cDNA? - (reply: 2)
1016. Comparing SYBR and probe-based Ct values - Is it valid? (reply: 2)
1017. qPCR inhibition - (reply: 1)
1018. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
1019. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
1020. Do low binding 0.2ml PCR tubes exist? - (reply: 3)