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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
991. Really weird amplification curves - (reply: 1)
992. Primers for qPCR - (reply: 3)
993. copy number calculation from cDNA - (reply: 1)
994. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
995. RT-PCR primer - (reply: 3)
996. CT value>25 with GAPDH in 50-fold dilution! - (reply: 3)
997. PCR problem - basic PCR for plasmid amplification (reply: 2)
998. can RNA amplify in two-step qPCR? - (reply: 2)
999. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
1000. Nested LA PCR - Any success? (reply: 3)
1001. Unpredictablity of PCR product - (reply: 5)
1002. total cDNA amplification by PCR - (reply: 2)
1003. Detection limit of a conventional PCR - (reply: 2)
1004. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
1005. RNAi showing upregulation via QPCR - Help needed (reply: 3)
1006. DNA detection limit of the PCR - Calculating detection limits (reply: 4)
1007. cDNA synthesis - (reply: 3)
1008. 2 rounds PCR got problem - (reply: 2)
1009. PCR help minus strand - Primer design (reply: 1)
1010. cDNA contamination, pls help! - (reply: 1)
1011. Smear in long distance PCR - (reply: 39)
1012. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
1013. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
1014. can contaminating gDNA explain this? - (reply: 1)
1015. TOUCH DOWN PCR - (reply: 2)
1016. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
1017. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
1018. PCR stopped working - After changing buffer (reply: 5)
1019. how to determine the amount of cDNA for qPCR - (reply: 5)
1020. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)