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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
991. Problems with semi-quatitative PCR - (reply: 1)
992. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
993. Long-amplicon QPCR of mtDNA - (reply: 5)
994. PCR reaction calculation - (reply: 2)
995. Qiagen rotorgene syber green kit reaction volume - (reply: 1)
996. missing nt at the 5' end - site mutagensis using NEB kit (reply: 1)
997. no increase in fluorescence in my real time PCR - (reply: 3)
998. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
999. Roche second derivative maximum - can be treated according to Pfaffl? (reply: 1)
1000. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
1001. Pooled RNA from healthy controls - delta-delta Ct method (reply: 1)
1002. Probe Dilution Problem = 100uM to 5ng/ul? + Final Volume for FISH - Probe dilution and FISH - Advise on volume for FISH hibridization buff (reply: 2)
1003. PCR, using gel extracted band as a template - (reply: 4)
1004. Question of nucleic acid extraction - (reply: 1)
1005. Multiplex problems - (reply: 6)
1006. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
1007. Q-PCR and RT-PCR - (reply: 3)
1008. inter-run calibration - (reply: 4)
1009. Delta Delta Ct method for multiple reference genes? - (reply: 1)
1010. FAM and VIC labelling - (reply: 2)
1011. Lost cause?? - I made a silly (reply: 4)
1012. how to use delta-delta Ct method? - when no expression in healthy controls! (reply: 2)
1013. confusing PCR - (reply: 1)
1014. difference between PCR primers and sequencing primers - (reply: 1)
1015. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
1016. making gel electrophoresis - (reply: 1)
1017. housekeeping genes in PCR - (reply: 1)
1018. Question about RealTime PCR Primer Design - (reply: 1)
1019. Primers stopped working!? - PCR Primers (reply: 6)
1020. NFK beta primers - (reply: 4)