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New Forum Archives (2009-): :
991. 18s as endogenous control for Oligo dT RT - Can we use 18s ? (reply: 2)
993. Reverse transcription (cDNA synthesis) curiosity and confusion - Does the amount of RNA have to be doubled if you double everything els (reply: 4)
995. Omitting Points in Standard Curve - (reply: 2)
997. Smallest reaction volume with Roche Lightcycler 480 96-well plate format - (reply: 2)
999. Primer dimer formation and real-time PCR - (reply: 7)
1001. Identify PCR products - (reply: 3)
1003. water-droplets in my cycler !!! - experienced such thing ??? (reply: 9)
1005. qPCR virgin, What do I do first? - How to set up experiment (reply: 1)
1007. Question about TM and Annealing temperatures - (reply: 4)
1009. PCR basics - I'm new to all things lab and need some help! (reply: 3)
1011. Is it acceptable that qpcr reaction doesn't reach plateau? - (reply: 3)
1013. quantitate wild type and mutant mRNA - (reply: 1)
1015. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
1017. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
1019. Using DNA as a reference in qRT-PCR - (reply: 1)
993. Reverse transcription (cDNA synthesis) curiosity and confusion - Does the amount of RNA have to be doubled if you double everything els (reply: 4)
995. Omitting Points in Standard Curve - (reply: 2)
997. Smallest reaction volume with Roche Lightcycler 480 96-well plate format - (reply: 2)
999. Primer dimer formation and real-time PCR - (reply: 7)
1001. Identify PCR products - (reply: 3)
1003. water-droplets in my cycler !!! - experienced such thing ??? (reply: 9)
1005. qPCR virgin, What do I do first? - How to set up experiment (reply: 1)
1007. Question about TM and Annealing temperatures - (reply: 4)
1009. PCR basics - I'm new to all things lab and need some help! (reply: 3)
1011. Is it acceptable that qpcr reaction doesn't reach plateau? - (reply: 3)
1013. quantitate wild type and mutant mRNA - (reply: 1)
1015. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
1017. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
1019. Using DNA as a reference in qRT-PCR - (reply: 1)