Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
991. PCR triplicates versus one reaction - differences? (reply: 17)
992. cloning of race products - (reply: 1)
993. Single or double-stranded cDNA? - (reply: 2)
994. Comparing SYBR and probe-based Ct values - Is it valid? (reply: 2)
995. qPCR inhibition - (reply: 1)
996. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
997. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
998. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
999. Primers and self and hetero dimers - (reply: 1)
1000. Really weird amplification curves - (reply: 1)
1001. Primers for qPCR - (reply: 3)
1002. copy number calculation from cDNA - (reply: 1)
1003. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
1004. RT-PCR primer - (reply: 3)
1005. CT value>25 with GAPDH in 50-fold dilution! - (reply: 3)
1006. PCR problem - basic PCR for plasmid amplification (reply: 2)
1007. can RNA amplify in two-step qPCR? - (reply: 2)
1008. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
1009. Nested LA PCR - Any success? (reply: 3)
1010. Unpredictablity of PCR product - (reply: 5)
1011. total cDNA amplification by PCR - (reply: 2)
1012. Detection limit of a conventional PCR - (reply: 2)
1013. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
1014. RNAi showing upregulation via QPCR - Help needed (reply: 3)
1015. DNA detection limit of the PCR - Calculating detection limits (reply: 4)
1016. cDNA synthesis - (reply: 3)
1017. 2 rounds PCR got problem - (reply: 2)
1018. PCR help minus strand - Primer design (reply: 1)
1019. cDNA contamination, pls help! - (reply: 1)
1020. Smear in long distance PCR - (reply: 39)