Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
991. Smallest reaction volume with Roche Lightcycler 480 96-well plate format - (reply: 2)
992. qPCR primer design - possible off-target (reply: 1)
993. Primer dimer formation and real-time PCR - (reply: 7)
994. 5' race - (reply: 2)
995. Identify PCR products - (reply: 3)
996. Maximum size of overhangs in PCR - (reply: 6)
997. water-droplets in my cycler !!! - experienced such thing ??? (reply: 9)
998. a second small peak to the right to my pricipal peak!!! - (reply: 1)
999. qPCR virgin, What do I do first? - How to set up experiment (reply: 1)
1000. Colony PCR doesn't work anymore - (reply: 5)
1001. Question about TM and Annealing temperatures - (reply: 4)
1002. RT-PCR t-test, and ANOVA - (reply: 1)
1003. PCR basics - I'm new to all things lab and need some help! (reply: 3)
1004. the red dye in trizol? - (reply: 2)
1005. Is it acceptable that qpcr reaction doesn't reach plateau? - (reply: 3)
1006. What are exogeneous and endogeneous controls? - (reply: 4)
1007. quantitate wild type and mutant mRNA - (reply: 1)
1008. Can very low RNA concentration cause Ct bias..? - (reply: 3)
1009. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
1010. iScript BioRad cDNA synthesis yield - (reply: 1)
1011. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
1012. intron spanning primers for non model organisms - (reply: 2)
1013. Using DNA as a reference in qRT-PCR - (reply: 1)
1014. minimum replicate for qpcr? - (reply: 15)
1015. Primer design - (reply: 2)
1016. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
1017. qPCR amplification interference fixed with freezing - (reply: 6)
1018. Suggest me a mastermix for conventinal PCR... - (reply: 11)
1019. reaction efficiency - (reply: 4)
1020. software for quantifing PCR product band - (reply: 1)