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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
991. the red dye in trizol? - (reply: 2)
992. Is it acceptable that qpcr reaction doesn't reach plateau? - (reply: 3)
993. What are exogeneous and endogeneous controls? - (reply: 4)
994. quantitate wild type and mutant mRNA - (reply: 1)
995. Can very low RNA concentration cause Ct bias..? - (reply: 3)
996. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
997. iScript BioRad cDNA synthesis yield - (reply: 1)
998. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
999. intron spanning primers for non model organisms - (reply: 2)
1000. Using DNA as a reference in qRT-PCR - (reply: 1)
1001. minimum replicate for qpcr? - (reply: 15)
1002. Primer design - (reply: 2)
1003. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
1004. qPCR amplification interference fixed with freezing - (reply: 6)
1005. Suggest me a mastermix for conventinal PCR... - (reply: 11)
1006. reaction efficiency - (reply: 4)
1007. software for quantifing PCR product band - (reply: 1)
1008. Thermal cycler - (reply: 2)
1009. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
1010. qPCR volume - Problem with volume difference and results (reply: 1)
1011. RT-PCR - (reply: 9)
1012. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
1013. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
1014. cDNA synthesis - (reply: 6)
1015. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
1016. Primer seq - (reply: 2)
1017. Selection of housekeeping gene in Mycobacterium - (reply: 1)
1018. Do I need to pool the base line samples together - (reply: 2)
1019. PCR product size - (reply: 6)
1020. amplification plot raises to early - What is the explanation for this problem? (reply: 1)