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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
991. qPCR volume - Problem with volume difference and results (reply: 1)
992. RT-PCR - (reply: 9)
993. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
994. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
995. cDNA synthesis - (reply: 6)
996. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
997. Primer seq - (reply: 2)
998. Selection of housekeeping gene in Mycobacterium - (reply: 1)
999. Do I need to pool the base line samples together - (reply: 2)
1000. PCR product size - (reply: 6)
1001. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
1002. Few basic questions about RNA - (reply: 3)
1003. oligo dt and random primer - (reply: 3)
1004. Ct's of goi higher and lower than ct's hkg - (reply: 1)
1005. Effect of collagenase on qpcr results - (reply: 1)
1006. RNA Isolation - (reply: 3)
1007. mRNA Search for RT-PCR (U to T) - (reply: 2)
1008. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
1009. PCR triplicates versus one reaction - differences? (reply: 17)
1010. cloning of race products - (reply: 1)
1011. Single or double-stranded cDNA? - (reply: 2)
1012. Comparing SYBR and probe-based Ct values - Is it valid? (reply: 2)
1013. qPCR inhibition - (reply: 1)
1014. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
1015. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
1016. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
1017. Primers and self and hetero dimers - (reply: 1)
1018. Really weird amplification curves - (reply: 1)
1019. Primers for qPCR - (reply: 3)
1020. copy number calculation from cDNA - (reply: 1)