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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
451. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
452. PCR primer usage (Clonning & cDNA) - (reply: 2)
453. Taq polymerase - (reply: 7)
454. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
455. Which High-Fidelity polymerase is better? - (reply: 2)
456. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
457. reference gene for qPCR in E.coli - (reply: 2)
458. Problem amplifying viral gene - (reply: 5)
459. The same CT with different Quantity, why? - (reply: 8)
460. Adding tag using overlapping PCR - (reply: 2)
461. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
462. Design primer from incomplete sequence... - (reply: 2)
463. Long term storage for RNA extracted from pancreas - (reply: 3)
464. How to perform colony PCR - (reply: 1)
465. qPCR from HEK cells transfected with Plasmid - (reply: 4)
466. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
467. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
468. Negative Control Looks Like the Rest - (reply: 11)
469. Why RNase H treatment after cDNA synthesis - (reply: 2)
470. RNA isolation from Sesame seed - (reply: 3)
471. qPCR with Genomic DNA - (reply: 1)
472. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
473. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
474. Homogenize pancreas for RNA extraction - (reply: 3)
475. Point mutagenesis..... - (reply: 6)
476. No bands in PCR after DNase treatment - (reply: 4)
477. Overlap PCR problem - (reply: 5)
478. PCR product purification - (reply: 4)
479. Overlapping sequence PCR primers - (reply: 1)
480. acceptable melt curve? - (reply: 1)