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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
451. Overlap PCR problem - (reply: 5)
452. PCR product purification - (reply: 4)
453. Overlapping sequence PCR primers - (reply: 1)
454. acceptable melt curve? - (reply: 1)
455. Calibrator sample - (reply: 4)
456. Ct value very high. What can I do to improve this ct? - (reply: 4)
457. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
458. Amount of RNA (ng) for good ct values. - (reply: 3)
459. Normalizing qPCR data - (reply: 1)
460. doing PCR using PCR product , help plz !! - (reply: 5)
461. High GC content RT-qPCR - please help - (reply: 3)
462. Electrophoresis problem , please help pals ! - (reply: 8)
463. Calculating Standard Errors and Normalising to Transgene - (reply: 1)
464. add template first or Syber mix first to reduce contamination in qPCR? - (reply: 3)
465. Strange looking "elevated" amplification plots from ChIP-qPCR samples - (reply: 2)
466. PCR-screening of T-DNA inserted in Arabidopsis gene - (reply: 1)
467. iCycler Calibration Question - (reply: 1)
468. Long PCR (>10kb) polymerase recommendations - (reply: 3)
469. thermocycler - real-time versus conventional? - (reply: 2)
470. I need help for designing a pair of primers for this sequence. - (reply: 1)
471. Change in Superscript lot influences transcript level of one gene but not others - (reply: 2)
472. 1/delta Ct vs delta delat CT - (reply: 1)
473. PCR band slightly BELOW expected length ?! - (reply: 14)
474. Does anyone have any problem with Quanta Sybr Green - (reply: 2)
475. Trouble correlating GU/ml qPCR results to cfu/ml plating results - (reply: 2)
476. long primers PCR - (reply: 4)
477. unexpected band in PCR with plasmid - (reply: 4)
478. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
479. Good Protocol for Semi-Quantitative RT-PCR? - (reply: 1)
480. DNA extraction - PCR Problem - (reply: 3)