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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
451. Amount of RNA (ng) for good ct values. - (reply: 3)
452. Normalizing qPCR data - (reply: 1)
453. doing PCR using PCR product , help plz !! - (reply: 5)
454. High GC content RT-qPCR - please help - (reply: 3)
455. Electrophoresis problem , please help pals ! - (reply: 8)
456. Calculating Standard Errors and Normalising to Transgene - (reply: 1)
457. add template first or Syber mix first to reduce contamination in qPCR? - (reply: 3)
458. Strange looking "elevated" amplification plots from ChIP-qPCR samples - (reply: 2)
459. PCR-screening of T-DNA inserted in Arabidopsis gene - (reply: 1)
460. iCycler Calibration Question - (reply: 1)
461. Long PCR (>10kb) polymerase recommendations - (reply: 3)
462. thermocycler - real-time versus conventional? - (reply: 2)
463. I need help for designing a pair of primers for this sequence. - (reply: 1)
464. Change in Superscript lot influences transcript level of one gene but not others - (reply: 2)
465. 1/delta Ct vs delta delat CT - (reply: 1)
466. PCR band slightly BELOW expected length ?! - (reply: 14)
467. Does anyone have any problem with Quanta Sybr Green - (reply: 2)
468. Trouble correlating GU/ml qPCR results to cfu/ml plating results - (reply: 2)
469. long primers PCR - (reply: 4)
470. unexpected band in PCR with plasmid - (reply: 4)
471. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
472. Good Protocol for Semi-Quantitative RT-PCR? - (reply: 1)
473. DNA extraction - PCR Problem - (reply: 3)
474. Real Time Trouble, low cTs - (reply: 3)
475. interpretation of results - (reply: 8)
476. Multiple Bands in qPCR - (reply: 4)
477. PCR product biotinylation - (reply: 3)
478. Tagged primers and a second PCR targetting the tags - (reply: 5)
479. Question about isolating DNA from the blood sample - (reply: 2)
480. RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR - (reply: 3)