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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
451. Problem amplifying viral gene - (reply: 5)
452. The same CT with different Quantity, why? - (reply: 8)
453. Adding tag using overlapping PCR - (reply: 2)
454. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
455. Design primer from incomplete sequence... - (reply: 2)
456. Long term storage for RNA extracted from pancreas - (reply: 3)
457. How to perform colony PCR - (reply: 1)
458. qPCR from HEK cells transfected with Plasmid - (reply: 4)
459. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
460. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
461. Negative Control Looks Like the Rest - (reply: 11)
462. Why RNase H treatment after cDNA synthesis - (reply: 2)
463. RNA isolation from Sesame seed - (reply: 3)
464. qPCR with Genomic DNA - (reply: 1)
465. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
466. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
467. Homogenize pancreas for RNA extraction - (reply: 3)
468. Point mutagenesis..... - (reply: 6)
469. No bands in PCR after DNase treatment - (reply: 4)
470. Overlap PCR problem - (reply: 5)
471. PCR product purification - (reply: 4)
472. Overlapping sequence PCR primers - (reply: 1)
473. acceptable melt curve? - (reply: 1)
474. Calibrator sample - (reply: 4)
475. Ct value very high. What can I do to improve this ct? - (reply: 4)
476. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
477. Amount of RNA (ng) for good ct values. - (reply: 3)
478. Normalizing qPCR data - (reply: 1)
479. doing PCR using PCR product , help plz !! - (reply: 5)
480. High GC content RT-qPCR - please help - (reply: 3)