Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
451. PYO pcr stopped working. - (reply: 1)
452. Bands seen with qPCR missing in regular PCR - (reply: 5)
453. PCR product one day, none the next day - (reply: 4)
454. PCR using CDNA as template - (reply: 2)
455. why cant get the band? - (reply: 4)
456. CT value too small - (reply: 1)
457. Need help amplifying repeating sequence. - (reply: 5)
458. RNA isolation from frozen cells - (reply: 2)
459. Another primer dimers problem - (reply: 23)
460. using PCR product as standard template - (reply: 3)
461. Amplification in water control but not in samples - (reply: 3)
462. PCR products form with DNA, but not with c-DNA - (reply: 2)
463. question about pcr amplification efficiency? - (reply: 1)
464. Which Cycler to buy? - Can you recommend any of these cyclers? (reply: 2)
465. a question about the annealing temperature - (reply: 6)
466. what's the function of ROX reference dye? - (reply: 2)
467. Gene Expression Issues - (reply: 3)
468. clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
469. No gene amplification, 18s amplification is fine - (reply: 1)
470. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
471. What can I do to increase the banding signal? - (reply: 6)
472. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
473. Tomato Actin PCR+Images included - (reply: 2)
474. single primer PCR - (reply: 5)
475. Weird qPCR curves - (reply: 4)
476. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
477. LacZ PCR Problems - (reply: 1)
478. Primer concentration - (reply: 4)
479. nested PCR with low target DNA? - (reply: 2)
480. RT-PCR calibrator - (reply: 2)