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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
451. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
452. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
453. Homogenize pancreas for RNA extraction - (reply: 3)
454. Point mutagenesis..... - (reply: 6)
455. No bands in PCR after DNase treatment - (reply: 4)
456. Overlap PCR problem - (reply: 5)
457. PCR product purification - (reply: 4)
458. Overlapping sequence PCR primers - (reply: 1)
459. acceptable melt curve? - (reply: 1)
460. Calibrator sample - (reply: 4)
461. Ct value very high. What can I do to improve this ct? - (reply: 4)
462. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
463. Amount of RNA (ng) for good ct values. - (reply: 3)
464. Normalizing qPCR data - (reply: 1)
465. doing PCR using PCR product , help plz !! - (reply: 5)
466. High GC content RT-qPCR - please help - (reply: 3)
467. Electrophoresis problem , please help pals ! - (reply: 8)
468. Calculating Standard Errors and Normalising to Transgene - (reply: 1)
469. add template first or Syber mix first to reduce contamination in qPCR? - (reply: 3)
470. Strange looking "elevated" amplification plots from ChIP-qPCR samples - (reply: 2)
471. PCR-screening of T-DNA inserted in Arabidopsis gene - (reply: 1)
472. iCycler Calibration Question - (reply: 1)
473. Long PCR (>10kb) polymerase recommendations - (reply: 3)
474. thermocycler - real-time versus conventional? - (reply: 2)
475. I need help for designing a pair of primers for this sequence. - (reply: 1)
476. Change in Superscript lot influences transcript level of one gene but not others - (reply: 2)
477. 1/delta Ct vs delta delat CT - (reply: 1)
478. PCR band slightly BELOW expected length ?! - (reply: 14)
479. Does anyone have any problem with Quanta Sybr Green - (reply: 2)
480. Trouble correlating GU/ml qPCR results to cfu/ml plating results - (reply: 2)