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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
451. Design primer from incomplete sequence... - (reply: 2)
452. Long term storage for RNA extracted from pancreas - (reply: 3)
453. How to perform colony PCR - (reply: 1)
454. qPCR from HEK cells transfected with Plasmid - (reply: 4)
455. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
456. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
457. Negative Control Looks Like the Rest - (reply: 11)
458. Why RNase H treatment after cDNA synthesis - (reply: 2)
459. RNA isolation from Sesame seed - (reply: 3)
460. qPCR with Genomic DNA - (reply: 1)
461. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
462. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
463. Homogenize pancreas for RNA extraction - (reply: 3)
464. Point mutagenesis..... - (reply: 6)
465. No bands in PCR after DNase treatment - (reply: 4)
466. Overlap PCR problem - (reply: 5)
467. PCR product purification - (reply: 4)
468. Overlapping sequence PCR primers - (reply: 1)
469. acceptable melt curve? - (reply: 1)
470. Calibrator sample - (reply: 4)
471. Ct value very high. What can I do to improve this ct? - (reply: 4)
472. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
473. Amount of RNA (ng) for good ct values. - (reply: 3)
474. Normalizing qPCR data - (reply: 1)
475. doing PCR using PCR product , help plz !! - (reply: 5)
476. High GC content RT-qPCR - please help - (reply: 3)
477. Electrophoresis problem , please help pals ! - (reply: 8)
478. Calculating Standard Errors and Normalising to Transgene - (reply: 1)
479. add template first or Syber mix first to reduce contamination in qPCR? - (reply: 3)
480. Strange looking "elevated" amplification plots from ChIP-qPCR samples - (reply: 2)