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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
451. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
452. Wierd Bands after PCR....Confused - (reply: 9)
453. PCR with Plasmid recovered from filter paper - (reply: 6)
454. PCR amplified product size - (reply: 5)
455. Opinion: What fold change is actually considered meaningful - (reply: 2)
456. Buffers RNase decontamination - (reply: 1)
457. the storage time for primers - (reply: 9)
458. Annealing temperature for PCR - (reply: 8)
459. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
460. Nested PCR - (reply: 2)
461. random primers or oligodT - (reply: 4)
462. Confused about normalization - (reply: 2)
463. To design or use published primers? - (reply: 4)
464. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
465. PCR primer usage (Clonning & cDNA) - (reply: 2)
466. Taq polymerase - (reply: 7)
467. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
468. Which High-Fidelity polymerase is better? - (reply: 2)
469. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
470. reference gene for qPCR in E.coli - (reply: 2)
471. Problem amplifying viral gene - (reply: 5)
472. The same CT with different Quantity, why? - (reply: 8)
473. Adding tag using overlapping PCR - (reply: 2)
474. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
475. Design primer from incomplete sequence... - (reply: 2)
476. Long term storage for RNA extracted from pancreas - (reply: 3)
477. How to perform colony PCR - (reply: 1)
478. qPCR from HEK cells transfected with Plasmid - (reply: 4)
479. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
480. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)