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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
451. PCR with Plasmid recovered from filter paper - (reply: 6)
452. PCR amplified product size - (reply: 5)
453. Opinion: What fold change is actually considered meaningful - (reply: 2)
454. Buffers RNase decontamination - (reply: 1)
455. the storage time for primers - (reply: 9)
456. Annealing temperature for PCR - (reply: 8)
457. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
458. Nested PCR - (reply: 2)
459. random primers or oligodT - (reply: 4)
460. Confused about normalization - (reply: 2)
461. To design or use published primers? - (reply: 4)
462. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
463. PCR primer usage (Clonning & cDNA) - (reply: 2)
464. Taq polymerase - (reply: 7)
465. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
466. Which High-Fidelity polymerase is better? - (reply: 2)
467. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
468. reference gene for qPCR in E.coli - (reply: 2)
469. Problem amplifying viral gene - (reply: 5)
470. The same CT with different Quantity, why? - (reply: 8)
471. Adding tag using overlapping PCR - (reply: 2)
472. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
473. Design primer from incomplete sequence... - (reply: 2)
474. Long term storage for RNA extracted from pancreas - (reply: 3)
475. How to perform colony PCR - (reply: 1)
476. qPCR from HEK cells transfected with Plasmid - (reply: 4)
477. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
478. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
479. Negative Control Looks Like the Rest - (reply: 11)
480. Why RNase H treatment after cDNA synthesis - (reply: 2)