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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
451. the storage time for primers - (reply: 9)
452. Annealing temperature for PCR - (reply: 8)
453. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
454. Nested PCR - (reply: 2)
455. random primers or oligodT - (reply: 4)
456. Confused about normalization - (reply: 2)
457. To design or use published primers? - (reply: 4)
458. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
459. PCR primer usage (Clonning & cDNA) - (reply: 2)
460. Taq polymerase - (reply: 7)
461. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
462. Which High-Fidelity polymerase is better? - (reply: 2)
463. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
464. reference gene for qPCR in E.coli - (reply: 2)
465. Problem amplifying viral gene - (reply: 5)
466. The same CT with different Quantity, why? - (reply: 8)
467. Adding tag using overlapping PCR - (reply: 2)
468. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
469. Design primer from incomplete sequence... - (reply: 2)
470. Long term storage for RNA extracted from pancreas - (reply: 3)
471. How to perform colony PCR - (reply: 1)
472. qPCR from HEK cells transfected with Plasmid - (reply: 4)
473. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
474. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
475. Negative Control Looks Like the Rest - (reply: 11)
476. Why RNase H treatment after cDNA synthesis - (reply: 2)
477. RNA isolation from Sesame seed - (reply: 3)
478. qPCR with Genomic DNA - (reply: 1)
479. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
480. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)