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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
451. PCR product purification - (reply: 4)
452. Overlapping sequence PCR primers - (reply: 1)
453. acceptable melt curve? - (reply: 1)
454. Calibrator sample - (reply: 4)
455. Ct value very high. What can I do to improve this ct? - (reply: 4)
456. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
457. Amount of RNA (ng) for good ct values. - (reply: 3)
458. Normalizing qPCR data - (reply: 1)
459. doing PCR using PCR product , help plz !! - (reply: 5)
460. High GC content RT-qPCR - please help - (reply: 3)
461. Electrophoresis problem , please help pals ! - (reply: 8)
462. Calculating Standard Errors and Normalising to Transgene - (reply: 1)
463. add template first or Syber mix first to reduce contamination in qPCR? - (reply: 3)
464. Strange looking "elevated" amplification plots from ChIP-qPCR samples - (reply: 2)
465. PCR-screening of T-DNA inserted in Arabidopsis gene - (reply: 1)
466. iCycler Calibration Question - (reply: 1)
467. Long PCR (>10kb) polymerase recommendations - (reply: 3)
468. thermocycler - real-time versus conventional? - (reply: 2)
469. I need help for designing a pair of primers for this sequence. - (reply: 1)
470. Change in Superscript lot influences transcript level of one gene but not others - (reply: 2)
471. 1/delta Ct vs delta delat CT - (reply: 1)
472. PCR band slightly BELOW expected length ?! - (reply: 14)
473. Does anyone have any problem with Quanta Sybr Green - (reply: 2)
474. Trouble correlating GU/ml qPCR results to cfu/ml plating results - (reply: 2)
475. long primers PCR - (reply: 4)
476. unexpected band in PCR with plasmid - (reply: 4)
477. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
478. Good Protocol for Semi-Quantitative RT-PCR? - (reply: 1)
479. DNA extraction - PCR Problem - (reply: 3)
480. Real Time Trouble, low cTs - (reply: 3)