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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
451. Problems with two step RT-PCR - (reply: 2)
452. Spliced and unspliced form of a gene - (reply: 3)
453. primer dilutions - (reply: 4)
454. Genotyping PCR primers - (reply: 1)
455. Standard curve using plasmid - (reply: 1)
456. How to compare these results? Please comment. - (reply: 5)
457. Primers too dilute - (reply: 1)
458. Amplification curves jagged - (reply: 1)
459. ddCT issue: no endogenous expression in control. - (reply: 4)
460. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
461. No PCR product in site-directed mutagenesis - (reply: 4)
462. Giardia/Crypto real time PCR late amplification - (reply: 1)
463. How to dilute the template DNA for PCR - (reply: 7)
464. Desired length of overlap region for OE-PCR - (reply: 6)
465. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
466. Need help for PCR - (reply: 5)
467. scorpion probe - (reply: 8)
468. Too low Ct value for reference gene !!! - (reply: 3)
469. unespecific band PCR from mouse genomic DNA - (reply: 9)
470. Help with primer design through PRIMER3 - (reply: 4)
471. Help with unknown band in restriction digest - (reply: 1)
472. how to chose right subsequent for her2(erbb2)gene ? - (reply: 3)
473. 454 sequencing - (reply: 2)
474. Preference for larger of 2 bands? - (reply: 2)
475. Tm of primers - prediction and verification - (reply: 1)
476. acceptable variation for biological replicate - (reply: 2)
477. RT-PCR contamination in negative control. - (reply: 1)
478. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
479. Technical problem with ABI7300 - (reply: 4)
480. cdna synthesis - (reply: 1)