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451. using cell line to generate standard curve - (reply: 6)
452. PCR DNA Concentration - (reply: 1)
453. 3' RACE creates too big band doesn't leave well - (reply: 1)
454. RT-PCR product- no band - (reply: 4)
455. Normalization factor - (reply: 3)
456. Understanding RACE PCR - (reply: 1)
457. SYBR Select Master Mix for CFX from Invitrogen - Storage - (reply: 4)
458. Dye for qPCR - (reply: 3)
459. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
460. Wierd Bands after PCR....Confused - (reply: 9)
461. PCR with Plasmid recovered from filter paper - (reply: 6)
462. PCR amplified product size - (reply: 5)
463. Opinion: What fold change is actually considered meaningful - (reply: 2)
464. Buffers RNase decontamination - (reply: 1)
465. the storage time for primers - (reply: 9)
466. Annealing temperature for PCR - (reply: 8)
467. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
468. Nested PCR - (reply: 2)
469. random primers or oligodT - (reply: 4)
470. Confused about normalization - (reply: 2)
471. To design or use published primers? - (reply: 4)
472. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
473. PCR primer usage (Clonning & cDNA) - (reply: 2)
474. Taq polymerase - (reply: 7)
475. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
476. Which High-Fidelity polymerase is better? - (reply: 2)
477. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
478. reference gene for qPCR in E.coli - (reply: 2)
479. Problem amplifying viral gene - (reply: 5)
480. The same CT with different Quantity, why? - (reply: 8)
452. PCR DNA Concentration - (reply: 1)
453. 3' RACE creates too big band doesn't leave well - (reply: 1)
454. RT-PCR product- no band - (reply: 4)
455. Normalization factor - (reply: 3)
456. Understanding RACE PCR - (reply: 1)
457. SYBR Select Master Mix for CFX from Invitrogen - Storage - (reply: 4)
458. Dye for qPCR - (reply: 3)
459. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
460. Wierd Bands after PCR....Confused - (reply: 9)
461. PCR with Plasmid recovered from filter paper - (reply: 6)
462. PCR amplified product size - (reply: 5)
463. Opinion: What fold change is actually considered meaningful - (reply: 2)
464. Buffers RNase decontamination - (reply: 1)
465. the storage time for primers - (reply: 9)
466. Annealing temperature for PCR - (reply: 8)
467. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
468. Nested PCR - (reply: 2)
469. random primers or oligodT - (reply: 4)
470. Confused about normalization - (reply: 2)
471. To design or use published primers? - (reply: 4)
472. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
473. PCR primer usage (Clonning & cDNA) - (reply: 2)
474. Taq polymerase - (reply: 7)
475. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
476. Which High-Fidelity polymerase is better? - (reply: 2)
477. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
478. reference gene for qPCR in E.coli - (reply: 2)
479. Problem amplifying viral gene - (reply: 5)
480. The same CT with different Quantity, why? - (reply: 8)