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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
451. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
452. Nested PCR - (reply: 2)
453. random primers or oligodT - (reply: 4)
454. Confused about normalization - (reply: 2)
455. To design or use published primers? - (reply: 4)
456. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
457. PCR primer usage (Clonning & cDNA) - (reply: 2)
458. Taq polymerase - (reply: 7)
459. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
460. Which High-Fidelity polymerase is better? - (reply: 2)
461. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
462. reference gene for qPCR in E.coli - (reply: 2)
463. Problem amplifying viral gene - (reply: 5)
464. The same CT with different Quantity, why? - (reply: 8)
465. Adding tag using overlapping PCR - (reply: 2)
466. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
467. Design primer from incomplete sequence... - (reply: 2)
468. Long term storage for RNA extracted from pancreas - (reply: 3)
469. How to perform colony PCR - (reply: 1)
470. qPCR from HEK cells transfected with Plasmid - (reply: 4)
471. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
472. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
473. Negative Control Looks Like the Rest - (reply: 11)
474. Why RNase H treatment after cDNA synthesis - (reply: 2)
475. RNA isolation from Sesame seed - (reply: 3)
476. qPCR with Genomic DNA - (reply: 1)
477. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
478. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
479. Homogenize pancreas for RNA extraction - (reply: 3)
480. Point mutagenesis..... - (reply: 6)