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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
451. Opinion: What fold change is actually considered meaningful - (reply: 2)
452. Buffers RNase decontamination - (reply: 1)
453. the storage time for primers - (reply: 9)
454. Annealing temperature for PCR - (reply: 8)
455. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
456. Nested PCR - (reply: 2)
457. random primers or oligodT - (reply: 4)
458. Confused about normalization - (reply: 2)
459. To design or use published primers? - (reply: 4)
460. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
461. PCR primer usage (Clonning & cDNA) - (reply: 2)
462. Taq polymerase - (reply: 7)
463. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
464. Which High-Fidelity polymerase is better? - (reply: 2)
465. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
466. reference gene for qPCR in E.coli - (reply: 2)
467. Problem amplifying viral gene - (reply: 5)
468. The same CT with different Quantity, why? - (reply: 8)
469. Adding tag using overlapping PCR - (reply: 2)
470. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
471. Design primer from incomplete sequence... - (reply: 2)
472. Long term storage for RNA extracted from pancreas - (reply: 3)
473. How to perform colony PCR - (reply: 1)
474. qPCR from HEK cells transfected with Plasmid - (reply: 4)
475. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
476. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
477. Negative Control Looks Like the Rest - (reply: 11)
478. Why RNase H treatment after cDNA synthesis - (reply: 2)
479. RNA isolation from Sesame seed - (reply: 3)
480. qPCR with Genomic DNA - (reply: 1)