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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
451. Why RNase H treatment after cDNA synthesis - (reply: 2)
452. RNA isolation from Sesame seed - (reply: 3)
453. qPCR with Genomic DNA - (reply: 1)
454. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
455. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
456. Homogenize pancreas for RNA extraction - (reply: 3)
457. Point mutagenesis..... - (reply: 6)
458. No bands in PCR after DNase treatment - (reply: 4)
459. Overlap PCR problem - (reply: 5)
460. PCR product purification - (reply: 4)
461. Overlapping sequence PCR primers - (reply: 1)
462. acceptable melt curve? - (reply: 1)
463. Calibrator sample - (reply: 4)
464. Ct value very high. What can I do to improve this ct? - (reply: 4)
465. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
466. Amount of RNA (ng) for good ct values. - (reply: 3)
467. Normalizing qPCR data - (reply: 1)
468. doing PCR using PCR product , help plz !! - (reply: 5)
469. High GC content RT-qPCR - please help - (reply: 3)
470. Electrophoresis problem , please help pals ! - (reply: 8)
471. Calculating Standard Errors and Normalising to Transgene - (reply: 1)
472. add template first or Syber mix first to reduce contamination in qPCR? - (reply: 3)
473. Strange looking "elevated" amplification plots from ChIP-qPCR samples - (reply: 2)
474. PCR-screening of T-DNA inserted in Arabidopsis gene - (reply: 1)
475. iCycler Calibration Question - (reply: 1)
476. Long PCR (>10kb) polymerase recommendations - (reply: 3)
477. thermocycler - real-time versus conventional? - (reply: 2)
478. I need help for designing a pair of primers for this sequence. - (reply: 1)
479. Change in Superscript lot influences transcript level of one gene but not others - (reply: 2)
480. 1/delta Ct vs delta delat CT - (reply: 1)