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1111. Primer efficiency test - (reply: 1)
1112. primer software - (reply: 2)
1113. Question on wired PCR - (reply: 1)
1114. Validation of housekeeping gene expression - (reply: 3)
1115. restriction digestion of PCR product - (reply: 7)
1116. reuse pipette tips - (reply: 2)
1117. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
1118. How to know in which exon a primer match? - (reply: 1)
1119. dUTPs in qRTPCR rxn - (reply: 1)
1120. Amplification of human genomic DNA - (reply: 2)
1121. Relative qPCR dCt analysis - Different ways to analyze data? (reply: 1)
1122. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
1123. make your own gotaq - (reply: 2)
1124. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
1125. isolation of RNA from insect cells - to isolate RNA from insect larvae and synthesize cDNA (reply: 2)
1126. primer RE over-hang nucleotides - common sequences? (reply: 5)
1127. Forward and reverse primers got very different Tm - what to do? (reply: 11)
1128. Does RT step smplify RNA - (reply: 2)
1129. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
1130. ROX utilization - (who uses ROX?) (reply: 1)
1131. qPCR analysis - (reply: 6)
1132. good housekeeping for ovary - (reply: 2)
1133. Can I use genomic DNA to make a standard curve for qPCR - (reply: 5)
1134. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
1135. primer design problem - (reply: 1)
1136. The perfect RT??? which control? - (reply: 4)
1137. Rotor Gene 3000 and Rotor Gene Q - (reply: 1)
1138. qPCR - qPCR problems.. (reply: 5)
1139. is it necessary to do DNases treatment? - (reply: 2)
1140. problem with site directed mutagenesis - (reply: 4)
1112. primer software - (reply: 2)
1113. Question on wired PCR - (reply: 1)
1114. Validation of housekeeping gene expression - (reply: 3)
1115. restriction digestion of PCR product - (reply: 7)
1116. reuse pipette tips - (reply: 2)
1117. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
1118. How to know in which exon a primer match? - (reply: 1)
1119. dUTPs in qRTPCR rxn - (reply: 1)
1120. Amplification of human genomic DNA - (reply: 2)
1121. Relative qPCR dCt analysis - Different ways to analyze data? (reply: 1)
1122. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
1123. make your own gotaq - (reply: 2)
1124. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
1125. isolation of RNA from insect cells - to isolate RNA from insect larvae and synthesize cDNA (reply: 2)
1126. primer RE over-hang nucleotides - common sequences? (reply: 5)
1127. Forward and reverse primers got very different Tm - what to do? (reply: 11)
1128. Does RT step smplify RNA - (reply: 2)
1129. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
1130. ROX utilization - (who uses ROX?) (reply: 1)
1131. qPCR analysis - (reply: 6)
1132. good housekeeping for ovary - (reply: 2)
1133. Can I use genomic DNA to make a standard curve for qPCR - (reply: 5)
1134. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
1135. primer design problem - (reply: 1)
1136. The perfect RT??? which control? - (reply: 4)
1137. Rotor Gene 3000 and Rotor Gene Q - (reply: 1)
1138. qPCR - qPCR problems.. (reply: 5)
1139. is it necessary to do DNases treatment? - (reply: 2)
1140. problem with site directed mutagenesis - (reply: 4)