Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1111. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
1112. qPCR for telomere length measurement - efficiency issues - complicated monochrome multiplex assay with SYBR Green (reply: 40)
1113. Nested PCR question - (reply: 1)
1114. High Tm on primers - cannot get a product - (reply: 8)
1115. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
1116. Help with Multiplex Nested PCR - (reply: 2)
1117. Relative quantification analysis Ct values & 2-ΔΔCT method - samples which do not amplify (reply: 5)
1118. trembling dissociation curves in HRM assays - (reply: 3)
1119. Fluorescein storage? - (reply: 2)
1120. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
1121. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
1122. TELO2 & GAPDH - (reply: 2)
1123. PCR -No band formation - (reply: 1)
1124. 3' race - (reply: 1)
1125. PCR AMPLIFICATION - (reply: 1)
1126. internal control for genomic DNA - (reply: 1)
1127. qPCR Data Analysis - Software (reply: 3)
1128. Gene expression confusion - (reply: 5)
1129. real time pcr need advise - (reply: 2)
1130. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
1131. ChIP-qPCR shift in melt curve - (reply: 4)
1132. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
1133. use of housekeeping gene in RT PCR chIP - (reply: 3)
1134. what is the order you follow in the run ??? - in order to make the contamination risk the least (reply: 3)
1135. DNA pooling for PCR - Saving money PCR (reply: 7)
1136. Real Time PCR Standard curves - How many are required??? (reply: 1)
1137. pcr-rflp - (reply: 2)
1138. PCR need some help - (reply: 4)
1139. problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)
1140. Left polymerase out overnight - Will it still work? (reply: 1)