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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1111. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
1112. making gel electrophoresis - (reply: 1)
1113. housekeeping genes in PCR - (reply: 1)
1114. Question about RealTime PCR Primer Design - (reply: 1)
1115. Primers stopped working!? - PCR Primers (reply: 6)
1116. NFK beta primers - (reply: 4)
1117. final PCR product - need clarifications (reply: 1)
1118. Trying to amplify the 3' end of Prolyl endopeptidse gene from cDNA.. need su - Hi, I'm trying to sequence a gene from the cDNA of an insect (reply: 2)
1119. Can I use Hot Start Taq polimerase for any target? - (reply: 6)
1120. Ct value and interpretation - (reply: 3)
1121. 16S rDNA - Bacteria identification (reply: 1)
1122. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
1123. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
1124. gene expressional analysis - (reply: 1)
1125. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
1126. Troubleshooting help: Why do my amplification curves look like this? - (reply: 8)
1127. why genomic can't be use for pcr? - (reply: 2)
1128. Re-use unamplified PCR product - (reply: 2)
1129. 96 well plate plan that converts into a list automatically - (reply: 2)
1130. Reason for odd PCR conditions - (reply: 3)
1131. pcr - problem in pcr (reply: 2)
1132. Primers for PCR - Forward and Reverse Primer Sequences (reply: 4)
1133. How to present and analyze these real-time PCR data? - (reply: 2)
1134. Deoxyribosenucleoside vs. Deoxyribosenucleotide - What does dNTP stand for? (reply: 2)
1135. RT-PCR: DNA contamination, one vs two step - RNA work (reply: 11)
1136. delta-delta-Ct method - what to do, when no expression in healthy control? (reply: 1)
1137. Trouble with annealing temperature - Trouble with annealing temperature (reply: 3)
1138. PCR Master mix - (reply: 2)
1139. Real Time PCR Primers and Probes - (reply: 2)
1140. Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)