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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1111. What does melting temperature indicate and how to predict annealing temperature - (reply: 9)
1112. 4 question for real time PCR - (reply: 5)
1113. RNA quality - (reply: 3)
1114. Superscript III - No second strand synthesis? - (reply: 1)
1115. cDNA convertion to DNA - (reply: 4)
1116. PCR didn't work - suspect the enzyme (reply: 2)
1117. How to check RNA quality - (reply: 5)
1118. Colony PCR Specificity - (reply: 2)
1119. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
1120. Reverse Transcriptase @ Room temperature for 48 hours - (reply: 9)
1121. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
1122. Primer Dilution Problem - D'oh! (reply: 2)
1123. Using PCR to create overhangs - (reply: 3)
1124. Site directed mutation - Changing D->K (reply: 2)
1125. Miner groove binder - urgent, help, what is it? (reply: 4)
1126. Problem with PCR on bacmids - (reply: 1)
1127. Copy number assay using Real time Pcr-SYBR green assay? - (reply: 3)
1128. FAM/HEX, lightcyler 480 - (reply: 1)
1129. Running real time pcr product on gel?.. - (reply: 4)
1130. help with ct of reference gene - (reply: 1)
1131. PCR mutagenesis of plasmid - (reply: 5)
1132. Primer designed for ARMS PCR - (reply: 2)
1133. Problem with Telomeric Length measurement using RT-PCR - (reply: 1)
1134. methylation specific PCR - Primers - (reply: 1)
1135. bacterial identification using real time PCR - (reply: 1)
1136. Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)
1137. real time PCR inhibition control - a test for the presence of PCR inhibitors (reply: 3)
1138. Problem with multiplex PCR - Troubleshooting (reply: 4)
1139. Questions about ChIP protocols - (reply: 1)
1140. PCR analysis method- delta or delta delta? - RNA, qRT PCR, delta Ct method (reply: 4)