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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1111. Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
1112. Dead genotyping PCR - I can't see s*** (reply: 5)
1113. Any one has light cycler 480 system operator's mannual? - Urgent!!!!!!!!!!!! (reply: 3)
1114. PCR product wrong size! Need help! - (reply: 9)
1115. pooling different pcr cycles? - (reply: 1)
1116. Amplification of concatenated linear ligated fragments - (reply: 4)
1117. Clinical Real-time PCR assay - (reply: 1)
1118. Alternative software to read .sds files? - (reply: 2)
1119. primer and promoter sequences - (reply: 1)
1120. Standard curve for real-time PCR - (reply: 2)
1121. delta Ct values - (reply: 1)
1122. Primer Tm differences/ PCR - (reply: 1)
1123. Interpretation of Ct values - (reply: 2)
1124. Primer design to screen silenced lines - Primer design tips to screen silenced lines (reply: 3)
1125. Question about RT... - (reply: 1)
1126. d2EGFP Primers for Genotyping - ...where to get the sequence? (reply: 5)
1127. PCR Primer Dilution from F+R - (reply: 1)
1128. Plasmid standard problem - (reply: 2)
1129. primer reconstitution - primer reconstitution (reply: 4)
1130. Not-Quite-Nested PCR - (reply: 2)
1131. Primer design for insert amplification - Is this correct? (reply: 2)
1132. got smear on the PCR gel !!! - (reply: 4)
1133. need to know minimum amount of template DNA needed for pcr amplification - pcr (reply: 2)
1134. Troubleshooting reverse transcriptase-PCR - (reply: 3)
1135. "Unzipped" PCR band - (reply: 3)
1136. Review on new amplification techniques - (reply: 2)
1137. melting temperature in real time pcr - melting temperature in real time pcr (reply: 5)
1138. Ct difference for the same amount of standards - (reply: 4)
1139. Puzzling Real Time Results - (reply: 1)
1140. Real-time qPCR data analysis - (reply: 1)