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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1111. internal control for genomic DNA - (reply: 1)
1112. qPCR Data Analysis - Software (reply: 3)
1113. Gene expression confusion - (reply: 5)
1114. real time pcr need advise - (reply: 2)
1115. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
1116. ChIP-qPCR shift in melt curve - (reply: 4)
1117. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
1118. use of housekeeping gene in RT PCR chIP - (reply: 3)
1119. what is the order you follow in the run ??? - in order to make the contamination risk the least (reply: 3)
1120. DNA pooling for PCR - Saving money PCR (reply: 7)
1121. Real Time PCR Standard curves - How many are required??? (reply: 1)
1122. pcr-rflp - (reply: 2)
1123. PCR need some help - (reply: 4)
1124. problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)
1125. Left polymerase out overnight - Will it still work? (reply: 1)
1126. very low expression of a sample vs standard curve efficiency - (reply: 1)
1127. Primer Reconstitution--does temperature matter? - (reply: 5)
1128. TaqMan Gene Expression Assays - Low expression/ Ct values (reply: 2)
1129. Gradient PCR works, but single temperature does not! HELP!! - (reply: 5)
1130. Oligonucleotide degradation - (reply: 1)
1131. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
1132. never get bands by pfu related enzymes - (reply: 2)
1133. Primer design for qPCR - (reply: 1)
1134. Primer-BLAST... - (reply: 4)
1135. reporting results of qPCR - (reply: 1)
1136. a question about 16s rRNA as reference in RT-qPCR - (reply: 1)
1137. PCR genomic DNA of high GC content - (reply: 6)
1138. question about qRT-PCR preparation - (reply: 1)
1139. GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
1140. qPCR efficiency calculation - (reply: 1)