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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1351. Validation of housekeeping gene expression - (reply: 3)
1352. restriction digestion of PCR product - (reply: 7)
1353. reuse pipette tips - (reply: 2)
1354. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
1355. How to know in which exon a primer match? - (reply: 1)
1356. dUTPs in qRTPCR rxn - (reply: 1)
1357. Amplification of human genomic DNA - (reply: 2)
1358. Relative qPCR dCt analysis - Different ways to analyze data? (reply: 1)
1359. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
1360. make your own gotaq - (reply: 2)
1361. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
1362. isolation of RNA from insect cells - to isolate RNA from insect larvae and synthesize cDNA (reply: 2)
1363. primer RE over-hang nucleotides - common sequences? (reply: 5)
1364. Forward and reverse primers got very different Tm - what to do? (reply: 11)
1365. Does RT step smplify RNA - (reply: 2)
1366. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
1367. ROX utilization - (who uses ROX?) (reply: 1)
1368. qPCR analysis - (reply: 6)
1369. good housekeeping for ovary - (reply: 2)
1370. Can I use genomic DNA to make a standard curve for qPCR - (reply: 5)
1371. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
1372. primer design problem - (reply: 1)
1373. The perfect RT??? which control? - (reply: 4)
1374. Rotor Gene 3000 and Rotor Gene Q - (reply: 1)
1375. qPCR - qPCR problems.. (reply: 5)
1376. is it necessary to do DNases treatment? - (reply: 2)
1377. problem with site directed mutagenesis - (reply: 4)
1378. allele specific pcr - determining genotypes (reply: 2)
1379. Low 260/230 ratio in my RNA - (reply: 3)
1380. Problems with GOI Ct's - How to do PCR efficiency test if there is no Ct in GOI? (reply: 7)