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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1351. qRT-PCR Product Size - (reply: 5)
1352. primer dimer - proplems (reply: 1)
1353. Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
1354. DATA ANALYSIS for qPCR - Calculating 2^(-Del Del Ct). (reply: 2)
1355. 18s control in qPCR - (reply: 2)
1356. weird PCR ask for help - (reply: 12)
1357. Absolute quantification and normalization - Are reference genes necessary? (reply: 1)
1358. What's the best electronic pipette (Precision) - (reply: 5)
1359. No PCR product at all - (reply: 8)
1360. PCR troubleshoooooting - primer dimer (reply: 1)
1361. Primer efficiency test - (reply: 1)
1362. primer software - (reply: 2)
1363. Question on wired PCR - (reply: 1)
1364. Validation of housekeeping gene expression - (reply: 3)
1365. restriction digestion of PCR product - (reply: 7)
1366. reuse pipette tips - (reply: 2)
1367. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
1368. How to know in which exon a primer match? - (reply: 1)
1369. dUTPs in qRTPCR rxn - (reply: 1)
1370. Amplification of human genomic DNA - (reply: 2)
1371. Relative qPCR dCt analysis - Different ways to analyze data? (reply: 1)
1372. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
1373. make your own gotaq - (reply: 2)
1374. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
1375. isolation of RNA from insect cells - to isolate RNA from insect larvae and synthesize cDNA (reply: 2)
1376. primer RE over-hang nucleotides - common sequences? (reply: 5)
1377. Forward and reverse primers got very different Tm - what to do? (reply: 11)
1378. Does RT step smplify RNA - (reply: 2)
1379. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
1380. ROX utilization - (who uses ROX?) (reply: 1)