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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1351. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
1352. Normalising RNA concentrations - Help (reply: 4)
1353. How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)
1354. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
1355. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
1356. Having abnormal dissociation curve when performing qPCR - (reply: 3)
1357. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
1358. Please help me if you could - (reply: 5)
1359. Tm caculation for different PCRs?? - new, confused, and frustrated beginner (reply: 3)
1360. absolute quantification - (reply: 4)
1361. The Case of a Missing Band: PCR Issue - (reply: 4)
1362. Log 10 fold serial dilutions - (reply: 2)
1363. paranormal qpcr amplification activity - melting curves (reply: 3)
1364. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
1365. PCR template concentration - (reply: 6)
1366. PCR efficiency calculated by Linreg - (reply: 3)
1367. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
1368. Stability of genomic DNA in 1x PBS - (reply: 2)
1369. primer design by generunner DG - (reply: 1)
1370. Calculation of Tm of amplicon when using SYBR Green - (reply: 3)
1371. degenerate pcr - (reply: 1)
1372. DNA degradation - (reply: 1)
1373. DNase 1 treratment of RNA - (reply: 11)
1374. Secondary structure in real time - (reply: 1)
1375. primers with fluoresent dye PCR cycle question - (reply: 4)
1376. House keeping gene question? - Thanks for any help! (reply: 1)
1377. Questions about Tween 20 - (reply: 1)
1378. General qRT-PCR question - (reply: 3)
1379. qPCR stats problem - Vandesompele's, DDct or other methods?? (reply: 1)
1380. Taqman probe not easily synthesized - What sequence features make probe synthesis difficult? (reply: 3)