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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1351. Stability of genomic DNA in 1x PBS - (reply: 2)
1352. primer design by generunner DG - (reply: 1)
1353. Calculation of Tm of amplicon when using SYBR Green - (reply: 3)
1354. degenerate pcr - (reply: 1)
1355. DNA degradation - (reply: 1)
1356. DNase 1 treratment of RNA - (reply: 11)
1357. Secondary structure in real time - (reply: 1)
1358. primers with fluoresent dye PCR cycle question - (reply: 4)
1359. House keeping gene question? - Thanks for any help! (reply: 1)
1360. Questions about Tween 20 - (reply: 1)
1361. General qRT-PCR question - (reply: 3)
1362. qPCR stats problem - Vandesompele's, DDct or other methods?? (reply: 1)
1363. Taqman probe not easily synthesized - What sequence features make probe synthesis difficult? (reply: 3)
1364. Smears on PCR products - (reply: 4)
1365. Dumb Reverse Transcription PCR Question - (reply: 2)
1366. qPCR absolute/relative quantification method question - (reply: 4)
1367. PCR-amplicon too short - (reply: 1)
1368. Reverse transcription PCR - (reply: 1)
1369. PCR/RT-PCR beads - (reply: 2)
1370. High Amplification Efficiency in Std. Curve - (reply: 5)
1371. Run PCR amplifications in agarose gels - (reply: 3)
1372. Problems with validating primers and low expression genes - (reply: 3)
1373. Compare different qPCR runs - really need help (reply: 8)
1374. General real time questions. - (reply: 1)
1375. Analysis HELP!!!! - (reply: 1)
1376. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
1377. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
1378. Mutagenesis woes - (reply: 3)
1379. KpnI Restriction enzyme - how to detect the SNP (reply: 1)
1380. intron/exon spanning primers - (reply: 1)