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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1351. Measuring gene expression across cell lines - (reply: 2)
1352. How to get enough product from limited samples without generating mutations? - (reply: 8)
1353. long range PCR - (reply: 2)
1354. ITS1/ITS4 and ITS1/ITS2 - (reply: 2)
1355. PCR off plasmid for screening - (reply: 2)
1356. BSA as qPCR enhancer - NEB or Sigma? (reply: 3)
1357. Primer clean-up - ExoSAP-It isn't working (reply: 6)
1358. Data acquisition for qPCR - Endpoint of annealing or endpoint of amplification? (reply: 3)
1359. Can't re-PCR the PCR product - (reply: 5)
1360. Contamination in Multilplex? - (reply: 4)
1361. some questions about the component tab of ABI prism 7000 software and the dissoc - (reply: 2)
1362. Optimal annealing temperature - (reply: 2)
1363. Little to no expression in one group, how to analyze this? - (reply: 1)
1364. Bad PCR, is it due to conditions, reagents, or lack of DNA template? - (reply: 1)
1365. Taqman real-time PCR - Taqman and contamination (reply: 4)
1366. silly control question - No RT controls (reply: 2)
1367. Long Primers for PCR - (reply: 2)
1368. Data Analysis for Real-time PCR - (reply: 2)
1369. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
1370. argh more pcr headaches! - (reply: 6)
1371. Annealing temperature - (reply: 3)
1372. Replicate inconsistencies in qPCR - (reply: 6)
1373. With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
1374. Chlamydia PCR cycling conditions - (reply: 1)
1375. On-unit screen or PC…which is better? - (reply: 12)
1376. Primers going "loopy" - (reply: 2)
1377. Biotinylated! - brief explanaion (reply: 2)
1378. what do i say?! - (reply: 2)
1379. Smearing on gel in PCR products - (reply: 5)
1380. 260/230 ratio: how low is good for running PCRs? - (reply: 2)