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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1351. Problems with validating primers and low expression genes - (reply: 3)
1352. Compare different qPCR runs - really need help (reply: 8)
1353. General real time questions. - (reply: 1)
1354. Analysis HELP!!!! - (reply: 1)
1355. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
1356. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
1357. Mutagenesis woes - (reply: 3)
1358. KpnI Restriction enzyme - how to detect the SNP (reply: 1)
1359. intron/exon spanning primers - (reply: 1)
1360. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
1361. PCR product for sequencing - sample storage?? (reply: 2)
1362. Absolute quantification using Real Time PCR - (reply: 2)
1363. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
1364. High Ct value - what to do next? - (reply: 4)
1365. Biorad Mini Opticon - can use any 0.2ml tubes or Biorad only? (reply: 1)
1366. RT-PCR Gel - Ladder looks terrible and product is fuzzy! (reply: 1)
1367. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
1368. Designing Primers for multiple Isoforms - (reply: 1)
1369. GAPDH CT lower than target gene CT - (reply: 2)
1370. standards for absolute quantification qPCR - (reply: 1)
1371. RT-PCR standard curve dilutions - (reply: 2)
1372. more than one band - (reply: 1)
1373. Relative gene expression - Newbie at RT-qPCR! (reply: 3)
1374. qPCR, RT- Control - (reply: 2)
1375. Rt-PCR problem !with 2.5 kb gene - (reply: 3)
1376. real time pcr melt curve and primer efficiency problem - (reply: 3)
1377. real time PCR - base line not zero anymore! (reply: 5)
1378. Pre-amplification of template for qPCR - (reply: 1)
1379. Efficiency estimation using a window of linearity - (reply: 3)
1380. Software for analysis of raw real-time flouresence data - Analysis of tab delimited text flouresence v cycle data (reply: 7)