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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1351. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
1352. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
1353. Having abnormal dissociation curve when performing qPCR - (reply: 3)
1354. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
1355. Please help me if you could - (reply: 5)
1356. Tm caculation for different PCRs?? - new, confused, and frustrated beginner (reply: 3)
1357. absolute quantification - (reply: 4)
1358. The Case of a Missing Band: PCR Issue - (reply: 4)
1359. Log 10 fold serial dilutions - (reply: 2)
1360. paranormal qpcr amplification activity - melting curves (reply: 3)
1361. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
1362. PCR template concentration - (reply: 6)
1363. PCR efficiency calculated by Linreg - (reply: 3)
1364. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
1365. Stability of genomic DNA in 1x PBS - (reply: 2)
1366. primer design by generunner DG - (reply: 1)
1367. Calculation of Tm of amplicon when using SYBR Green - (reply: 3)
1368. degenerate pcr - (reply: 1)
1369. DNA degradation - (reply: 1)
1370. DNase 1 treratment of RNA - (reply: 11)
1371. Secondary structure in real time - (reply: 1)
1372. primers with fluoresent dye PCR cycle question - (reply: 4)
1373. House keeping gene question? - Thanks for any help! (reply: 1)
1374. Questions about Tween 20 - (reply: 1)
1375. General qRT-PCR question - (reply: 3)
1376. qPCR stats problem - Vandesompele's, DDct or other methods?? (reply: 1)
1377. Taqman probe not easily synthesized - What sequence features make probe synthesis difficult? (reply: 3)
1378. Smears on PCR products - (reply: 4)
1379. Dumb Reverse Transcription PCR Question - (reply: 2)
1380. qPCR absolute/relative quantification method question - (reply: 4)