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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1351. 260/230 ratio: how low is good for running PCRs? - (reply: 2)
1352. introducing alanine mutations - (reply: 1)
1353. Hematin - calculation (reply: 1)
1354. How to generate sticky ends? - (reply: 3)
1355. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
1356. PROBLEM WITH MELTING CURVES - i realy need help (reply: 2)
1357. Relative RT-PCR, multiplate and calibration - (reply: 4)
1358. Trying to measure small levels of mRNA - (reply: 3)
1359. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
1360. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
1361. Relative RT-PCR - (reply: 2)
1362. Relative real time RT PCR - (reply: 2)
1363. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
1364. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
1365. IQ5 and sybr/FAM - (reply: 3)
1366. a simple primer problem - (reply: 5)
1367. Failure to introduce mutations using Overlap PCR - (reply: 3)
1368. no or poor amplification - (reply: 1)
1369. Primers from 3'UTR - (reply: 2)
1370. Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
1371. Designing primers with ESTs - (reply: 2)
1372. Ct variation/Ct limit/rē values/efficiency errors - (reply: 2)
1373. Probe manufacturers, who makes best probes? - Where do you get your probes and are you happy with them? (reply: 3)
1374. Real Time vs Traditional PCR results - (reply: 1)
1375. The problems to identify mouse genotype with PCR - (reply: 1)
1376. non specific amplification - DNA amplification by PCR (reply: 1)
1377. RNA Extraction - Extraction of Yeast RNA from mice blood (reply: 1)
1378. Large Difference between Ct of 18s rRNA and Target Gene - (reply: 2)
1379. PCR has stopped working - (reply: 7)
1380. PCR negative control contamination - (reply: 8)