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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1351. PROBLEM WITH MELTING CURVES - i realy need help (reply: 2)
1352. Relative RT-PCR, multiplate and calibration - (reply: 4)
1353. Trying to measure small levels of mRNA - (reply: 3)
1354. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
1355. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
1356. Relative RT-PCR - (reply: 2)
1357. Relative real time RT PCR - (reply: 2)
1358. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
1359. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
1360. IQ5 and sybr/FAM - (reply: 3)
1361. a simple primer problem - (reply: 5)
1362. Failure to introduce mutations using Overlap PCR - (reply: 3)
1363. no or poor amplification - (reply: 1)
1364. Primers from 3'UTR - (reply: 2)
1365. Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
1366. Designing primers with ESTs - (reply: 2)
1367. Ct variation/Ct limit/rē values/efficiency errors - (reply: 2)
1368. Probe manufacturers, who makes best probes? - Where do you get your probes and are you happy with them? (reply: 3)
1369. Real Time vs Traditional PCR results - (reply: 1)
1370. The problems to identify mouse genotype with PCR - (reply: 1)
1371. non specific amplification - DNA amplification by PCR (reply: 1)
1372. RNA Extraction - Extraction of Yeast RNA from mice blood (reply: 1)
1373. Large Difference between Ct of 18s rRNA and Target Gene - (reply: 2)
1374. PCR has stopped working - (reply: 7)
1375. PCR negative control contamination - (reply: 8)
1376. Real-Time PCR - Gene expression (reply: 2)
1377. PCR efficiency in real timePCR - (reply: 5)
1378. Plate setup for qRT-PCR - Too many samples for one plate... (reply: 1)
1379. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
1380. QPCR analysis with various # of samples from 1 patient? - (reply: 1)