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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1351. qPCR absolute/relative quantification method question - (reply: 4)
1352. PCR-amplicon too short - (reply: 1)
1353. Reverse transcription PCR - (reply: 1)
1354. PCR/RT-PCR beads - (reply: 2)
1355. High Amplification Efficiency in Std. Curve - (reply: 5)
1356. Run PCR amplifications in agarose gels - (reply: 3)
1357. Problems with validating primers and low expression genes - (reply: 3)
1358. Compare different qPCR runs - really need help (reply: 8)
1359. General real time questions. - (reply: 1)
1360. Analysis HELP!!!! - (reply: 1)
1361. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
1362. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
1363. Mutagenesis woes - (reply: 3)
1364. KpnI Restriction enzyme - how to detect the SNP (reply: 1)
1365. intron/exon spanning primers - (reply: 1)
1366. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
1367. PCR product for sequencing - sample storage?? (reply: 2)
1368. Absolute quantification using Real Time PCR - (reply: 2)
1369. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
1370. High Ct value - what to do next? - (reply: 4)
1371. Biorad Mini Opticon - can use any 0.2ml tubes or Biorad only? (reply: 1)
1372. RT-PCR Gel - Ladder looks terrible and product is fuzzy! (reply: 1)
1373. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
1374. Designing Primers for multiple Isoforms - (reply: 1)
1375. GAPDH CT lower than target gene CT - (reply: 2)
1376. standards for absolute quantification qPCR - (reply: 1)
1377. RT-PCR standard curve dilutions - (reply: 2)
1378. more than one band - (reply: 1)
1379. Relative gene expression - Newbie at RT-qPCR! (reply: 3)
1380. qPCR, RT- Control - (reply: 2)