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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1351. Urgent Help needed: RNA-Interference, rt-pcr and Western-Blot do not match - (reply: 2)
1352. Running trays cracking - (reply: 4)
1353. Premix vendor dependent disssociation curves? - (reply: 3)
1354. Human 18s rRNA - Human 18s rRNA and qRTPCR normalization (reply: 7)
1355. Which Plasmid DNA for standard curve? - (reply: 5)
1356. standard curve/ PCR efficiency problems... - (reply: 1)
1357. Why is the y-axis labeled 'relative gene expression'? - (reply: 5)
1358. Reverse transcription question - Volume for RT reaction (reply: 3)
1359. Help! PCR that used to work doesn't work now! - (reply: 2)
1360. PCR and templates - (reply: 3)
1361. DeltaDelta Ct method and Statistics - (reply: 2)
1362. always run internal control for different genes on the differnet plates? - (reply: 8)
1363. HELP! Genomic DNA, RNA-size bands - (reply: 2)
1364. Real Time PCR internal reference (housekeeping) gene in E. coli - is rrsB suitable (reply: 1)
1365. Easy primer question? - Primers (reply: 1)
1366. PCR inconsistency - (reply: 4)
1367. help needed: PCR a gene from genomic DNA - (reply: 2)
1368. Does centrifugual speed of Rotor Gene-Q have to calibration? - (reply: 1)
1369. questions about pcr products after pooling - (reply: 3)
1370. stiching/linking/sewing pcr - (reply: 3)
1371. Human mtDNA amplification problems - (reply: 3)
1372. qRT-PCR Product Size - (reply: 5)
1373. primer dimer - proplems (reply: 1)
1374. Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
1375. DATA ANALYSIS for qPCR - Calculating 2^(-Del Del Ct). (reply: 2)
1376. 18s control in qPCR - (reply: 2)
1377. weird PCR ask for help - (reply: 12)
1378. Absolute quantification and normalization - Are reference genes necessary? (reply: 1)
1379. What's the best electronic pipette (Precision) - (reply: 5)
1380. No PCR product at all - (reply: 8)