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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
31. Primer calculation for qPCR - final reaction - (reply: 1)
32. Sec. structure in primers - (reply: 1)
33. Differences in shape of melt curve - (reply: 4)
34. Amount of RNA for cDNA - (reply: 4)
35. Difference in melting temperature - (reply: 5)
36. Changing PCR recipe halfway - (reply: 1)
37. PCR master mix containing primers and Taq - (reply: 4)
38. Primer working with some cDNA samples and not other - (reply: 2)
39. no bands after pcr!!!! - (reply: 4)
40. Help with definitons for qPCR - (reply: 1)
41. Calibrator in qPCR - (reply: 7)
42. Experimental design for RT-PCR - (reply: 8)
43. Primer dilution - (reply: 7)
44. Calculation help - (reply: 6)
45. standards for qPCR and some other doubts - (reply: 9)
46. Plasma cortisol/corticosterone level analysis using qPCR - (reply: 1)
47. Technical Replicates vs Biological Replicates - (reply: 1)
48. pcr - (reply: 1)
49. Difference in synthesis - (reply: 1)
50. Standardizing cDNA concentration before doing PCR - (reply: 4)
51. Agarose Gel Electrophoresis - (reply: 1)
52. Volume of Primers - (reply: 4)
53. qPCR with different genes - (reply: 5)
54. PCR conditions unknown? - (reply: 10)
55. PCR anomaly in a mid-range dilution of the template - (reply: 2)
56. Reducing contamination in 16s PCR for metagenomic library prer - (reply: 4)
57. 2 peaks for identical sequences in HRM - (reply: 2)
58. PCR using very long oligos !!! - (reply: 4)
59. Housekeeping gene for Real Time PCR when comparing expression of genes across di - (reply: 1)
60. How to optimize a master mix kit? - (reply: 7)