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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
31. Combining standard error of normalised fold change values - Qbase method - (reply: 2)
32. What cycle is best for analysing data? - (reply: 2)
33. Mineral oil quality in PCR tubes - (reply: 5)
34. Internal control for absolute quantification - (reply: 3)
35. Diagnostic PCR troubleshooting - (reply: 2)
36. QPCR replicates issue - (reply: 1)
37. RT-PCR Kit's Validation - (reply: 1)
38. Rt pcr - (reply: 4)
39. Viral Quantification - (reply: 1)
40. Inhibition of PCR amplification of bacterial genomic DNA by RNA - (reply: 1)
41. Housekeeping genes - Cq values - (reply: 4)
42. typical mRNA test with huge fold change? Any suggestions - (reply: 1)
43. PCR Amplification Issues and Primer dimer - (reply: 4)
44. Two consecutive ATG - (reply: 2)
45. How to make PCR analysis using the 22DDCT Method - (reply: 1)
46. qPCR data analysis from raw data - (reply: 1)
47. cDNA degradation within a week at -20? - (reply: 2)
48. Primer calculation for qPCR - final reaction - (reply: 1)
49. Sec. structure in primers - (reply: 1)
50. Differences in shape of melt curve - (reply: 4)
51. Amount of RNA for cDNA - (reply: 4)
52. Difference in melting temperature - (reply: 5)
53. Changing PCR recipe halfway - (reply: 1)
54. PCR master mix containing primers and Taq - (reply: 4)
55. Primer working with some cDNA samples and not other - (reply: 2)
56. no bands after pcr!!!! - (reply: 4)
57. Help with definitons for qPCR - (reply: 1)
58. Calibrator in qPCR - (reply: 7)
59. Experimental design for RT-PCR - (reply: 8)
60. Primer dilution - (reply: 7)