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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
31. Problematic DNA Extraction - (reply: 1)
32. Length of amplicon for RT real time PCR - (reply: 2)
33. The curve of sample one is ideal or not - (reply: 1)
34. REAL TIME PCR WITH TAQMAN PROBE - (reply: 1)
35. Optimal dNTP storage pH - (reply: 1)
36. Recombinant DNA vaccine production - (reply: 9)
37. Amount of cDNA input in RT-PCR - (reply: 1)
38. Housekeeper Genes and Real-Time PCR - (reply: 1)
39. Pre-designed qRT-PCR primers - (reply: 1)
40. Same primers and different melting peaks for two samples (~1C appart) - (reply: 2)
41. Gene expression in colonic tissues using qRT-PCR - (reply: 1)
42. qPCR run w. strong positive NTC signal but very low fluorescence - (reply: 1)
43. Designing primers with his-tag for pET28a+ vector - (reply: 3)
44. making your own qPCR kit! - (reply: 1)
45. Problem with TaqMan qPCR efficiency - (reply: 1)
46. High molecular weight band from my PCR - (reply: 3)
47. relative quantification - things to do before the actual qPCR - (reply: 3)
48. is it how total cDNA should look like? - (reply: 3)
49. testing primers efficiency for rt-PCR - (reply: 1)
50. -RT contamination problem in PCR - (reply: 5)
51. Strange Taqman probe negative fluorescence - (reply: 2)
52. qPCR std curve calculation issue if using PCR product as the std - (reply: 1)
53. qPCR , eDNA and standard curve - (reply: 3)
54. Thermal Cycler for Multiplex PCR - (reply: 3)
55. Problem of melt curve shape for pikoreal real time PCR - (reply: 1)
56. Primer sequences with M and Y - how to order? - (reply: 2)
57. QPCR with genomic DNA from blood - (reply: 2)
58. RT-PCR low efficiency - (reply: 1)
59. emulsion PCR - (reply: 1)
60. qPCR data analysis - (reply: 2)