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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
31. PCR contamination - (reply: 6)
32. RNA agarose gel blurry fuzzy. RNase contaminaton? - (reply: 2)
33. cDNA serial dilution trouble in qPCR: help needed - (reply: 2)
34. High background fluorescence detected before starting PCR - (reply: 3)
35. Real-time pcr - (reply: 1)
36. Using qPCR machine for simple PCR, is it possible? - (reply: 3)
37. What could be the possible reasons for a RT-PCR experiment that was working fine - (reply: 4)
38. Combining standard error of normalised fold change values - Qbase method - (reply: 2)
39. What cycle is best for analysing data? - (reply: 2)
40. Mineral oil quality in PCR tubes - (reply: 5)
41. Internal control for absolute quantification - (reply: 3)
42. Diagnostic PCR troubleshooting - (reply: 2)
43. QPCR replicates issue - (reply: 1)
44. RT-PCR Kit's Validation - (reply: 1)
45. Rt pcr - (reply: 4)
46. Viral Quantification - (reply: 1)
47. Inhibition of PCR amplification of bacterial genomic DNA by RNA - (reply: 1)
48. Housekeeping genes - Cq values - (reply: 4)
49. typical mRNA test with huge fold change? Any suggestions - (reply: 1)
50. PCR Amplification Issues and Primer dimer - (reply: 4)
51. Two consecutive ATG - (reply: 2)
52. How to make PCR analysis using the 22DDCT Method - (reply: 1)
53. qPCR data analysis from raw data - (reply: 1)
54. cDNA degradation within a week at -20? - (reply: 2)
55. Primer calculation for qPCR - final reaction - (reply: 1)
56. Sec. structure in primers - (reply: 1)
57. Differences in shape of melt curve - (reply: 4)
58. Amount of RNA for cDNA - (reply: 4)
59. Difference in melting temperature - (reply: 5)
60. Changing PCR recipe halfway - (reply: 1)