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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
31. Strange Taqman probe negative fluorescence - (reply: 2)
32. qPCR std curve calculation issue if using PCR product as the std - (reply: 1)
33. qPCR , eDNA and standard curve - (reply: 3)
34. Thermal Cycler for Multiplex PCR - (reply: 3)
35. Problem of melt curve shape for pikoreal real time PCR - (reply: 1)
36. Primer sequences with M and Y - how to order? - (reply: 2)
37. QPCR with genomic DNA from blood - (reply: 2)
38. RT-PCR low efficiency - (reply: 1)
39. emulsion PCR - (reply: 1)
40. qPCR data analysis - (reply: 2)
41. Addition of DNase after RNA extraction - (reply: 4)
42. RT-PCR electrophorasis - (reply: 1)
43. PCR contamination - (reply: 6)
44. RNA agarose gel blurry fuzzy. RNase contaminaton? - (reply: 2)
45. cDNA serial dilution trouble in qPCR: help needed - (reply: 2)
46. High background fluorescence detected before starting PCR - (reply: 3)
47. Real-time pcr - (reply: 1)
48. Using qPCR machine for simple PCR, is it possible? - (reply: 3)
49. What could be the possible reasons for a RT-PCR experiment that was working fine - (reply: 4)
50. Combining standard error of normalised fold change values - Qbase method - (reply: 2)
51. What cycle is best for analysing data? - (reply: 2)
52. Mineral oil quality in PCR tubes - (reply: 5)
53. Internal control for absolute quantification - (reply: 3)
54. Diagnostic PCR troubleshooting - (reply: 2)
55. QPCR replicates issue - (reply: 1)
56. RT-PCR Kit's Validation - (reply: 1)
57. Rt pcr - (reply: 4)
58. Viral Quantification - (reply: 1)
59. Inhibition of PCR amplification of bacterial genomic DNA by RNA - (reply: 1)
60. Housekeeping genes - Cq values - (reply: 4)