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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
31. qPCR data analysis - (reply: 2)
32. Addition of DNase after RNA extraction - (reply: 4)
33. RT-PCR electrophorasis - (reply: 1)
34. PCR contamination - (reply: 6)
35. RNA agarose gel blurry fuzzy. RNase contaminaton? - (reply: 2)
36. cDNA serial dilution trouble in qPCR: help needed - (reply: 2)
37. High background fluorescence detected before starting PCR - (reply: 3)
38. Real-time pcr - (reply: 1)
39. Using qPCR machine for simple PCR, is it possible? - (reply: 3)
40. What could be the possible reasons for a RT-PCR experiment that was working fine - (reply: 4)
41. Combining standard error of normalised fold change values - Qbase method - (reply: 2)
42. What cycle is best for analysing data? - (reply: 2)
43. Mineral oil quality in PCR tubes - (reply: 5)
44. Internal control for absolute quantification - (reply: 3)
45. Diagnostic PCR troubleshooting - (reply: 2)
46. QPCR replicates issue - (reply: 1)
47. RT-PCR Kit's Validation - (reply: 1)
48. Rt pcr - (reply: 4)
49. Viral Quantification - (reply: 1)
50. Inhibition of PCR amplification of bacterial genomic DNA by RNA - (reply: 1)
51. Housekeeping genes - Cq values - (reply: 4)
52. typical mRNA test with huge fold change? Any suggestions - (reply: 1)
53. PCR Amplification Issues and Primer dimer - (reply: 4)
54. Two consecutive ATG - (reply: 2)
55. How to make PCR analysis using the 22DDCT Method - (reply: 1)
56. qPCR data analysis from raw data - (reply: 1)
57. cDNA degradation within a week at -20? - (reply: 2)
58. Primer calculation for qPCR - final reaction - (reply: 1)
59. Sec. structure in primers - (reply: 1)
60. Differences in shape of melt curve - (reply: 4)