Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
31. Gene expression in colonic tissues using qRT-PCR - (reply: 1)
32. qPCR run w. strong positive NTC signal but very low fluorescence - (reply: 1)
33. Designing primers with his-tag for pET28a+ vector - (reply: 3)
34. making your own qPCR kit! - (reply: 1)
35. Problem with TaqMan qPCR efficiency - (reply: 1)
36. High molecular weight band from my PCR - (reply: 3)
37. relative quantification - things to do before the actual qPCR - (reply: 3)
38. is it how total cDNA should look like? - (reply: 3)
39. testing primers efficiency for rt-PCR - (reply: 1)
40. -RT contamination problem in PCR - (reply: 5)
41. Strange Taqman probe negative fluorescence - (reply: 2)
42. qPCR std curve calculation issue if using PCR product as the std - (reply: 1)
43. qPCR , eDNA and standard curve - (reply: 3)
44. Thermal Cycler for Multiplex PCR - (reply: 3)
45. Problem of melt curve shape for pikoreal real time PCR - (reply: 1)
46. Primer sequences with M and Y - how to order? - (reply: 2)
47. QPCR with genomic DNA from blood - (reply: 2)
48. RT-PCR low efficiency - (reply: 1)
49. emulsion PCR - (reply: 1)
50. qPCR data analysis - (reply: 2)
51. Addition of DNase after RNA extraction - (reply: 4)
52. RT-PCR electrophorasis - (reply: 1)
53. PCR contamination - (reply: 6)
54. RNA agarose gel blurry fuzzy. RNase contaminaton? - (reply: 2)
55. cDNA serial dilution trouble in qPCR: help needed - (reply: 2)
56. High background fluorescence detected before starting PCR - (reply: 3)
57. Real-time pcr - (reply: 1)
58. Using qPCR machine for simple PCR, is it possible? - (reply: 3)
59. What could be the possible reasons for a RT-PCR experiment that was working fine - (reply: 4)
60. Combining standard error of normalised fold change values - Qbase method - (reply: 2)