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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
31. Difference in synthesis - (reply: 1)
32. Standardizing cDNA concentration before doing PCR - (reply: 4)
33. Agarose Gel Electrophoresis - (reply: 1)
34. Volume of Primers - (reply: 4)
35. qPCR with different genes - (reply: 5)
36. PCR conditions unknown? - (reply: 10)
37. PCR anomaly in a mid-range dilution of the template - (reply: 2)
38. Reducing contamination in 16s PCR for metagenomic library prer - (reply: 4)
39. 2 peaks for identical sequences in HRM - (reply: 2)
40. PCR using very long oligos !!! - (reply: 4)
41. Housekeeping gene for Real Time PCR when comparing expression of genes across di - (reply: 1)
42. How to optimize a master mix kit? - (reply: 7)
43. pcr primer design - (reply: 6)
44. Please help (primer/ probe final volume calculations) - (reply: 1)
45. Primer design with EcoRI ends - (reply: 2)
46. qPCR normalization - (reply: 4)
47. Reference Gene/HKG- every plate - (reply: 2)
48. qPCR standard for lipase genes - (reply: 2)
49. PCR with overlapping primers only (primer extention) - (reply: 3)
50. Random chuck of nonsense in middle of PCR - (reply: 6)
51. Campylobacter jejuni qPCR targeting VS1 gene - (reply: 2)
52. RT-PCR analysis - (reply: 1)
53. Newbie to qPCR - (reply: 4)
54. real time qPCR standard range plasmid - (reply: 3)
55. New to PCR - (reply: 3)
56. How can I check the quality of bacterial primers and their specificity on blast? - (reply: 1)
57. Who can advise me a kit that would allow me to purify amplicons from quantitativ - (reply: 3)
58. 3-primer PCR Genotyping - problem in hemizygotes - (reply: 2)
59. t-test & qRT-PCR - (reply: 1)
60. Primer design - (reply: 1)