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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
31. Finding an answer as to why I have Tm but no Ct - (reply: 6)
32. Amplification in HK gene but not for target gene - (reply: 3)
33. Missing DNA Target and Effect on Non-Specific Amplicons - (reply: 1)
34. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
35. how to make this reaction? - (reply: 1)
36. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
37. How long can I store at - 30C my PCR mix? - (reply: 6)
38. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
39. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
40. RT-PCR help - (reply: 2)
41. Strange signals in the NTC - (reply: 1)
42. False positive - help - (reply: 3)
43. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
44. PCR Temperature change control malfunctioning - (reply: 1)
45. Assay question - (reply: 1)
46. WHY NO ONE REPLIES, is this a stupid question ? - (reply: 5)
47. HRM instrumentaion - (reply: 4)
48. Can you repeat a thermocycle? - (reply: 3)
49. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
50. Amplification curve in the negative control samples - (reply: 7)
51. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
52. Primer as limiting reagent in PCR reaction - (reply: 2)
53. Taqman probe in a LightCycler 480? - (reply: 1)
54. Real time PCR for degraded RNA - (reply: 1)
55. Ct value of housekeeping gene - (reply: 4)
56. Efficiency correct fold change -- use all or none? - (reply: 1)
57. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
58. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
59. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
60. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)