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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
31. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
32. RT-PCR help - (reply: 2)
33. Strange signals in the NTC - (reply: 1)
34. False positive - help - (reply: 3)
35. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
36. PCR Temperature change control malfunctioning - (reply: 1)
37. Assay question - (reply: 1)
38. WHY NO ONE REPLIES, is this a stupid question ? - (reply: 5)
39. HRM instrumentaion - (reply: 4)
40. Can you repeat a thermocycle? - (reply: 3)
41. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
42. Amplification curve in the negative control samples - (reply: 7)
43. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
44. Primer as limiting reagent in PCR reaction - (reply: 2)
45. Taqman probe in a LightCycler 480? - (reply: 1)
46. Real time PCR for degraded RNA - (reply: 1)
47. Ct value of housekeeping gene - (reply: 4)
48. Efficiency correct fold change -- use all or none? - (reply: 1)
49. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
50. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
51. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
52. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
53. Is this primer-dimer peak in my melting curve? - (reply: 6)
54. Taq / Phusion mix - (reply: 3)
55. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
56. Dilution series between HKG and GOI - (reply: 7)
57. Reference gene(s) selection and validaton for qPCR - (reply: 2)
58. Is that normal the negative control showing signals? - (reply: 13)
59. qPCR cannot detect reaction. - (reply: 1)
60. website for primer design - (reply: 1)