Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
31. problem with PCR amplification of cDNA - (reply: 4)
32. Funny shaped PCR curve - (reply: 1)
33. How clean do I need my DNA to be? - (reply: 1)
34. Problematic DNA Extraction - (reply: 1)
35. Length of amplicon for RT real time PCR - (reply: 2)
36. The curve of sample one is ideal or not - (reply: 1)
37. REAL TIME PCR WITH TAQMAN PROBE - (reply: 1)
38. Optimal dNTP storage pH - (reply: 1)
39. Recombinant DNA vaccine production - (reply: 9)
40. Amount of cDNA input in RT-PCR - (reply: 1)
41. Housekeeper Genes and Real-Time PCR - (reply: 1)
42. Pre-designed qRT-PCR primers - (reply: 1)
43. Same primers and different melting peaks for two samples (~1C appart) - (reply: 2)
44. Gene expression in colonic tissues using qRT-PCR - (reply: 1)
45. qPCR run w. strong positive NTC signal but very low fluorescence - (reply: 1)
46. Designing primers with his-tag for pET28a+ vector - (reply: 3)
47. making your own qPCR kit! - (reply: 1)
48. Problem with TaqMan qPCR efficiency - (reply: 1)
49. High molecular weight band from my PCR - (reply: 3)
50. relative quantification - things to do before the actual qPCR - (reply: 3)
51. is it how total cDNA should look like? - (reply: 3)
52. testing primers efficiency for rt-PCR - (reply: 1)
53. -RT contamination problem in PCR - (reply: 5)
54. Strange Taqman probe negative fluorescence - (reply: 2)
55. qPCR std curve calculation issue if using PCR product as the std - (reply: 1)
56. qPCR , eDNA and standard curve - (reply: 3)
57. Thermal Cycler for Multiplex PCR - (reply: 3)
58. Problem of melt curve shape for pikoreal real time PCR - (reply: 1)
59. Primer sequences with M and Y - how to order? - (reply: 2)
60. QPCR with genomic DNA from blood - (reply: 2)