Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
31. Inhibition of PCR amplification of bacterial genomic DNA by RNA - (reply: 1)
32. Housekeeping genes - Cq values - (reply: 4)
33. typical mRNA test with huge fold change? Any suggestions - (reply: 1)
34. PCR Amplification Issues and Primer dimer - (reply: 4)
35. Two consecutive ATG - (reply: 2)
36. How to make PCR analysis using the 22DDCT Method - (reply: 1)
37. qPCR data analysis from raw data - (reply: 1)
38. cDNA degradation within a week at -20? - (reply: 2)
39. Primer calculation for qPCR - final reaction - (reply: 1)
40. Sec. structure in primers - (reply: 1)
41. Differences in shape of melt curve - (reply: 4)
42. Amount of RNA for cDNA - (reply: 4)
43. Difference in melting temperature - (reply: 5)
44. Changing PCR recipe halfway - (reply: 1)
45. PCR master mix containing primers and Taq - (reply: 4)
46. Primer working with some cDNA samples and not other - (reply: 2)
47. no bands after pcr!!!! - (reply: 4)
48. Help with definitons for qPCR - (reply: 1)
49. Calibrator in qPCR - (reply: 7)
50. Experimental design for RT-PCR - (reply: 8)
51. Primer dilution - (reply: 7)
52. Calculation help - (reply: 6)
53. standards for qPCR and some other doubts - (reply: 9)
54. Plasma cortisol/corticosterone level analysis using qPCR - (reply: 1)
55. Technical Replicates vs Biological Replicates - (reply: 1)
56. pcr - (reply: 1)
57. Difference in synthesis - (reply: 1)
58. Standardizing cDNA concentration before doing PCR - (reply: 4)
59. Agarose Gel Electrophoresis - (reply: 1)
60. Volume of Primers - (reply: 4)