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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
31. Degenerated Primers and their problems - (reply: 1)
32. Interpreting primer BLAST scores for self-complementary - (reply: 5)
33. Testing primers on 'unknown' tissue - (reply: 1)
34. Second Hand Roche Lightcycler II maching - to buy or not to buy - (reply: 1)
35. nested PCR for low viral load- HBV patient sample - (reply: 2)
36. Finding an answer as to why I have Tm but no Ct - (reply: 6)
37. Amplification in HK gene but not for target gene - (reply: 3)
38. Missing DNA Target and Effect on Non-Specific Amplicons - (reply: 1)
39. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
40. how to make this reaction? - (reply: 1)
41. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
42. How long can I store at - 30C my PCR mix? - (reply: 6)
43. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
44. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
45. RT-PCR help - (reply: 2)
46. Strange signals in the NTC - (reply: 1)
47. False positive - help - (reply: 3)
48. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
49. PCR Temperature change control malfunctioning - (reply: 1)
50. Assay question - (reply: 1)
51. WHY NO ONE REPLIES, is this a stupid question ? - (reply: 5)
52. HRM instrumentaion - (reply: 4)
53. Can you repeat a thermocycle? - (reply: 3)
54. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
55. Amplification curve in the negative control samples - (reply: 7)
56. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
57. Primer as limiting reagent in PCR reaction - (reply: 2)
58. Taqman probe in a LightCycler 480? - (reply: 1)
59. Real time PCR for degraded RNA - (reply: 1)
60. Ct value of housekeeping gene - (reply: 4)