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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
31. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
32. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
33. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
34. Is this primer-dimer peak in my melting curve? - (reply: 6)
35. Taq / Phusion mix - (reply: 3)
36. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
37. Dilution series between HKG and GOI - (reply: 7)
38. Reference gene(s) selection and validaton for qPCR - (reply: 1)
39. Is that normal the negative control showing signals? - (reply: 13)
40. qPCR cannot detect reaction. - (reply: 1)
41. website for primer design - (reply: 1)
42. PCR Efficiency over 150%! - (reply: 1)
43. problem of amplification - (reply: 2)
44. Rxn not working - amplify/linearize plasmid - (reply: 3)
45. qRT-PCR works one day, and not the next - (reply: 1)
46. PCR not working - (reply: 11)
47. an urgent problem with replicates - (reply: 10)
48. Site-directed mutagenesis: maximum substitution - (reply: 1)
49. Inter run calibration qRT-PCR - (reply: 1)
50. Primer design and alternative transcripts - (reply: 2)
51. qPCR amplification - (reply: 4)
52. Extremely desperate noob question: How do these PCR work? - (reply: 6)
53. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
54. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)
55. RT-PCR - High Ct Values and Laser Capture - (reply: 1)
56. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
57. Genomic DNA Isolation from Soft Agar Colonies - (reply: 3)
58. help with total RNA isolation - (reply: 1)
59. Need help to identify bands in AGE result - (reply: 3)
60. COLD-PCR (Dissociation Curve) via qPCR - (reply: 4)