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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
31. Problem with TaqMan qPCR efficiency - (reply: 1)
32. High molecular weight band from my PCR - (reply: 3)
33. relative quantification - things to do before the actual qPCR - (reply: 3)
34. is it how total cDNA should look like? - (reply: 3)
35. testing primers efficiency for rt-PCR - (reply: 1)
36. -RT contamination problem in PCR - (reply: 5)
37. Strange Taqman probe negative fluorescence - (reply: 2)
38. qPCR std curve calculation issue if using PCR product as the std - (reply: 1)
39. qPCR , eDNA and standard curve - (reply: 3)
40. Thermal Cycler for Multiplex PCR - (reply: 3)
41. Problem of melt curve shape for pikoreal real time PCR - (reply: 1)
42. Primer sequences with M and Y - how to order? - (reply: 2)
43. QPCR with genomic DNA from blood - (reply: 2)
44. RT-PCR low efficiency - (reply: 1)
45. emulsion PCR - (reply: 1)
46. qPCR data analysis - (reply: 2)
47. Addition of DNase after RNA extraction - (reply: 4)
48. RT-PCR electrophorasis - (reply: 1)
49. PCR contamination - (reply: 6)
50. RNA agarose gel blurry fuzzy. RNase contaminaton? - (reply: 2)
51. cDNA serial dilution trouble in qPCR: help needed - (reply: 2)
52. High background fluorescence detected before starting PCR - (reply: 3)
53. Real-time pcr - (reply: 1)
54. Using qPCR machine for simple PCR, is it possible? - (reply: 3)
55. What could be the possible reasons for a RT-PCR experiment that was working fine - (reply: 4)
56. Combining standard error of normalised fold change values - Qbase method - (reply: 2)
57. What cycle is best for analysing data? - (reply: 2)
58. Mineral oil quality in PCR tubes - (reply: 5)
59. Internal control for absolute quantification - (reply: 3)
60. Diagnostic PCR troubleshooting - (reply: 2)