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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
31. High background fluorescence detected before starting PCR - (reply: 3)
32. Real-time pcr - (reply: 1)
33. Using qPCR machine for simple PCR, is it possible? - (reply: 3)
34. What could be the possible reasons for a RT-PCR experiment that was working fine - (reply: 4)
35. Combining standard error of normalised fold change values - Qbase method - (reply: 2)
36. What cycle is best for analysing data? - (reply: 2)
37. Mineral oil quality in PCR tubes - (reply: 5)
38. Internal control for absolute quantification - (reply: 3)
39. Diagnostic PCR troubleshooting - (reply: 2)
40. QPCR replicates issue - (reply: 1)
41. RT-PCR Kit's Validation - (reply: 1)
42. Rt pcr - (reply: 4)
43. Viral Quantification - (reply: 1)
44. Inhibition of PCR amplification of bacterial genomic DNA by RNA - (reply: 1)
45. Housekeeping genes - Cq values - (reply: 4)
46. typical mRNA test with huge fold change? Any suggestions - (reply: 1)
47. PCR Amplification Issues and Primer dimer - (reply: 4)
48. Two consecutive ATG - (reply: 2)
49. How to make PCR analysis using the 22DDCT Method - (reply: 1)
50. qPCR data analysis from raw data - (reply: 1)
51. cDNA degradation within a week at -20? - (reply: 2)
52. Primer calculation for qPCR - final reaction - (reply: 1)
53. Sec. structure in primers - (reply: 1)
54. Differences in shape of melt curve - (reply: 4)
55. Amount of RNA for cDNA - (reply: 4)
56. Difference in melting temperature - (reply: 5)
57. Changing PCR recipe halfway - (reply: 1)
58. PCR master mix containing primers and Taq - (reply: 4)
59. Primer working with some cDNA samples and not other - (reply: 2)
60. no bands after pcr!!!! - (reply: 4)