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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
31. Optimal dNTP storage pH - (reply: 1)
32. Recombinant DNA vaccine production - (reply: 9)
33. Amount of cDNA input in RT-PCR - (reply: 1)
34. Housekeeper Genes and Real-Time PCR - (reply: 1)
35. Pre-designed qRT-PCR primers - (reply: 1)
36. Same primers and different melting peaks for two samples (~1C appart) - (reply: 2)
37. Gene expression in colonic tissues using qRT-PCR - (reply: 1)
38. qPCR run w. strong positive NTC signal but very low fluorescence - (reply: 1)
39. Designing primers with his-tag for pET28a+ vector - (reply: 3)
40. making your own qPCR kit! - (reply: 1)
41. Problem with TaqMan qPCR efficiency - (reply: 1)
42. High molecular weight band from my PCR - (reply: 3)
43. relative quantification - things to do before the actual qPCR - (reply: 3)
44. is it how total cDNA should look like? - (reply: 3)
45. testing primers efficiency for rt-PCR - (reply: 1)
46. -RT contamination problem in PCR - (reply: 5)
47. Strange Taqman probe negative fluorescence - (reply: 2)
48. qPCR std curve calculation issue if using PCR product as the std - (reply: 1)
49. qPCR , eDNA and standard curve - (reply: 3)
50. Thermal Cycler for Multiplex PCR - (reply: 3)
51. Problem of melt curve shape for pikoreal real time PCR - (reply: 1)
52. Primer sequences with M and Y - how to order? - (reply: 2)
53. QPCR with genomic DNA from blood - (reply: 2)
54. RT-PCR low efficiency - (reply: 1)
55. emulsion PCR - (reply: 1)
56. qPCR data analysis - (reply: 2)
57. Addition of DNase after RNA extraction - (reply: 4)
58. RT-PCR electrophorasis - (reply: 1)
59. PCR contamination - (reply: 6)
60. RNA agarose gel blurry fuzzy. RNase contaminaton? - (reply: 2)