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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
31. What could be the possible reasons for a RT-PCR experiment that was working fine - (reply: 4)
32. Combining standard error of normalised fold change values - Qbase method - (reply: 2)
33. What cycle is best for analysing data? - (reply: 2)
34. Mineral oil quality in PCR tubes - (reply: 5)
35. Internal control for absolute quantification - (reply: 3)
36. Diagnostic PCR troubleshooting - (reply: 2)
37. QPCR replicates issue - (reply: 1)
38. RT-PCR Kit's Validation - (reply: 1)
39. Rt pcr - (reply: 4)
40. Viral Quantification - (reply: 1)
41. Inhibition of PCR amplification of bacterial genomic DNA by RNA - (reply: 1)
42. Housekeeping genes - Cq values - (reply: 4)
43. typical mRNA test with huge fold change? Any suggestions - (reply: 1)
44. PCR Amplification Issues and Primer dimer - (reply: 4)
45. Two consecutive ATG - (reply: 2)
46. How to make PCR analysis using the 22DDCT Method - (reply: 1)
47. qPCR data analysis from raw data - (reply: 1)
48. cDNA degradation within a week at -20? - (reply: 2)
49. Primer calculation for qPCR - final reaction - (reply: 1)
50. Sec. structure in primers - (reply: 1)
51. Differences in shape of melt curve - (reply: 4)
52. Amount of RNA for cDNA - (reply: 4)
53. Difference in melting temperature - (reply: 5)
54. Changing PCR recipe halfway - (reply: 1)
55. PCR master mix containing primers and Taq - (reply: 4)
56. Primer working with some cDNA samples and not other - (reply: 2)
57. no bands after pcr!!!! - (reply: 4)
58. Help with definitons for qPCR - (reply: 1)
59. Calibrator in qPCR - (reply: 7)
60. Experimental design for RT-PCR - (reply: 8)