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31. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
32. Nested PCR - (reply: 2)
33. random primers or oligodT - (reply: 4)
34. Confused about normalization - (reply: 2)
35. To design or use published primers? - (reply: 4)
36. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
37. PCR primer usage (Clonning & cDNA) - (reply: 2)
38. Taq polymerase - (reply: 7)
39. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
40. Which High-Fidelity polymerase is better? - (reply: 2)
41. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
42. reference gene for qPCR in E.coli - (reply: 1)
43. Problem amplifying viral gene - (reply: 5)
44. The same CT with different Quantity, why? - (reply: 8)
45. Adding tag using overlapping PCR - (reply: 2)
46. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
47. Design primer from incomplete sequence... - (reply: 2)
48. Long term storage for RNA extracted from pancreas - (reply: 3)
49. How to perform colony PCR - (reply: 1)
50. qPCR from HEK cells transfected with Plasmid - (reply: 4)
51. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
52. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
53. Negative Control Looks Like the Rest - (reply: 11)
54. Why RNase H treatment after cDNA synthesis - (reply: 2)
55. RNA isolation from Sesame seed - (reply: 3)
56. qPCR with Genomic DNA - (reply: 1)
57. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
58. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
59. Homogenize pancreas for RNA extraction - (reply: 3)
60. Point mutagenesis..... - (reply: 5)
32. Nested PCR - (reply: 2)
33. random primers or oligodT - (reply: 4)
34. Confused about normalization - (reply: 2)
35. To design or use published primers? - (reply: 4)
36. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
37. PCR primer usage (Clonning & cDNA) - (reply: 2)
38. Taq polymerase - (reply: 7)
39. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
40. Which High-Fidelity polymerase is better? - (reply: 2)
41. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
42. reference gene for qPCR in E.coli - (reply: 1)
43. Problem amplifying viral gene - (reply: 5)
44. The same CT with different Quantity, why? - (reply: 8)
45. Adding tag using overlapping PCR - (reply: 2)
46. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
47. Design primer from incomplete sequence... - (reply: 2)
48. Long term storage for RNA extracted from pancreas - (reply: 3)
49. How to perform colony PCR - (reply: 1)
50. qPCR from HEK cells transfected with Plasmid - (reply: 4)
51. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
52. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
53. Negative Control Looks Like the Rest - (reply: 11)
54. Why RNase H treatment after cDNA synthesis - (reply: 2)
55. RNA isolation from Sesame seed - (reply: 3)
56. qPCR with Genomic DNA - (reply: 1)
57. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
58. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
59. Homogenize pancreas for RNA extraction - (reply: 3)
60. Point mutagenesis..... - (reply: 5)