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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
421. Opinion: What fold change is actually considered meaningful - (reply: 2)
422. Buffers RNase decontamination - (reply: 1)
423. the storage time for primers - (reply: 9)
424. Annealing temperature for PCR - (reply: 8)
425. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
426. Nested PCR - (reply: 2)
427. random primers or oligodT - (reply: 4)
428. Confused about normalization - (reply: 2)
429. To design or use published primers? - (reply: 4)
430. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
431. PCR primer usage (Clonning & cDNA) - (reply: 2)
432. Taq polymerase - (reply: 7)
433. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
434. Which High-Fidelity polymerase is better? - (reply: 2)
435. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
436. reference gene for qPCR in E.coli - (reply: 2)
437. Problem amplifying viral gene - (reply: 5)
438. The same CT with different Quantity, why? - (reply: 8)
439. Adding tag using overlapping PCR - (reply: 2)
440. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
441. Design primer from incomplete sequence... - (reply: 2)
442. Long term storage for RNA extracted from pancreas - (reply: 3)
443. How to perform colony PCR - (reply: 1)
444. qPCR from HEK cells transfected with Plasmid - (reply: 4)
445. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
446. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
447. Negative Control Looks Like the Rest - (reply: 11)
448. Why RNase H treatment after cDNA synthesis - (reply: 2)
449. RNA isolation from Sesame seed - (reply: 3)
450. qPCR with Genomic DNA - (reply: 1)