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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
421. Wierd Bands after PCR....Confused - (reply: 9)
422. PCR with Plasmid recovered from filter paper - (reply: 6)
423. PCR amplified product size - (reply: 5)
424. Opinion: What fold change is actually considered meaningful - (reply: 2)
425. Buffers RNase decontamination - (reply: 1)
426. the storage time for primers - (reply: 9)
427. Annealing temperature for PCR - (reply: 8)
428. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
429. Nested PCR - (reply: 2)
430. random primers or oligodT - (reply: 4)
431. Confused about normalization - (reply: 2)
432. To design or use published primers? - (reply: 4)
433. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
434. PCR primer usage (Clonning & cDNA) - (reply: 2)
435. Taq polymerase - (reply: 7)
436. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
437. Which High-Fidelity polymerase is better? - (reply: 2)
438. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
439. reference gene for qPCR in E.coli - (reply: 2)
440. Problem amplifying viral gene - (reply: 5)
441. The same CT with different Quantity, why? - (reply: 8)
442. Adding tag using overlapping PCR - (reply: 2)
443. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
444. Design primer from incomplete sequence... - (reply: 2)
445. Long term storage for RNA extracted from pancreas - (reply: 3)
446. How to perform colony PCR - (reply: 1)
447. qPCR from HEK cells transfected with Plasmid - (reply: 4)
448. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
449. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
450. Negative Control Looks Like the Rest - (reply: 11)