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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
421. Understanding RACE PCR - (reply: 1)
422. SYBR Select Master Mix for CFX from Invitrogen - Storage - (reply: 4)
423. Dye for qPCR - (reply: 3)
424. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
425. Wierd Bands after PCR....Confused - (reply: 9)
426. PCR with Plasmid recovered from filter paper - (reply: 6)
427. PCR amplified product size - (reply: 5)
428. Opinion: What fold change is actually considered meaningful - (reply: 2)
429. Buffers RNase decontamination - (reply: 1)
430. the storage time for primers - (reply: 9)
431. Annealing temperature for PCR - (reply: 8)
432. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
433. Nested PCR - (reply: 2)
434. random primers or oligodT - (reply: 4)
435. Confused about normalization - (reply: 2)
436. To design or use published primers? - (reply: 4)
437. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
438. PCR primer usage (Clonning & cDNA) - (reply: 2)
439. Taq polymerase - (reply: 7)
440. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
441. Which High-Fidelity polymerase is better? - (reply: 2)
442. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
443. reference gene for qPCR in E.coli - (reply: 2)
444. Problem amplifying viral gene - (reply: 5)
445. The same CT with different Quantity, why? - (reply: 8)
446. Adding tag using overlapping PCR - (reply: 2)
447. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
448. Design primer from incomplete sequence... - (reply: 2)
449. Long term storage for RNA extracted from pancreas - (reply: 3)
450. How to perform colony PCR - (reply: 1)