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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
421. RT-PCR product- no band - (reply: 4)
422. Normalization factor - (reply: 3)
423. Understanding RACE PCR - (reply: 1)
424. SYBR Select Master Mix for CFX from Invitrogen - Storage - (reply: 4)
425. Dye for qPCR - (reply: 3)
426. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
427. Wierd Bands after PCR....Confused - (reply: 9)
428. PCR with Plasmid recovered from filter paper - (reply: 6)
429. PCR amplified product size - (reply: 5)
430. Opinion: What fold change is actually considered meaningful - (reply: 2)
431. Buffers RNase decontamination - (reply: 1)
432. the storage time for primers - (reply: 9)
433. Annealing temperature for PCR - (reply: 8)
434. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
435. Nested PCR - (reply: 2)
436. random primers or oligodT - (reply: 4)
437. Confused about normalization - (reply: 2)
438. To design or use published primers? - (reply: 4)
439. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
440. PCR primer usage (Clonning & cDNA) - (reply: 2)
441. Taq polymerase - (reply: 7)
442. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
443. Which High-Fidelity polymerase is better? - (reply: 2)
444. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
445. reference gene for qPCR in E.coli - (reply: 2)
446. Problem amplifying viral gene - (reply: 5)
447. The same CT with different Quantity, why? - (reply: 8)
448. Adding tag using overlapping PCR - (reply: 2)
449. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
450. Design primer from incomplete sequence... - (reply: 2)