Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
421. How to prepare serial dilutions of RNA or cDNA - (reply: 3)
422. Are these primer products good enough for qPCR? - (reply: 3)
423. mtDNA to nDNA Ratio gDNA - qPCR - (reply: 1)
424. Can't get PCR with large overhang primers to work - (reply: 8)
425. Primer Efficiency across runs - (reply: 1)
426. Question about pipet tips for PCR and rtPCR - (reply: 4)
427. Taqman Probe sequence problem - (reply: 4)
428. UNG in PCR - (reply: 1)
429. Cause of random samples failing PCR? - (reply: 2)
430. Template DNA for PCR- Concrentration or Volume?? - (reply: 1)
431. RNA extraction - (reply: 3)
432. Normalization of Ct of interest to a reference Ct - (reply: 3)
433. urgent: need help with qPCR statistics - (reply: 2)
434. A question for Reverse transcription experts - (reply: 5)
435. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
436. PCR amplification with new restriction sites troubleshooting - (reply: 2)
437. using cell line to generate standard curve - (reply: 6)
438. PCR DNA Concentration - (reply: 1)
439. 3' RACE creates too big band doesn't leave well - (reply: 1)
440. RT-PCR product- no band - (reply: 4)
441. Normalization factor - (reply: 3)
442. Understanding RACE PCR - (reply: 1)
443. SYBR Select Master Mix for CFX from Invitrogen - Storage - (reply: 4)
444. Dye for qPCR - (reply: 3)
445. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
446. Wierd Bands after PCR....Confused - (reply: 9)
447. PCR with Plasmid recovered from filter paper - (reply: 6)
448. PCR amplified product size - (reply: 5)
449. Opinion: What fold change is actually considered meaningful - (reply: 2)
450. Buffers RNase decontamination - (reply: 1)