Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
421. Which High-Fidelity polymerase is better? - (reply: 2)
422. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
423. reference gene for qPCR in E.coli - (reply: 2)
424. Problem amplifying viral gene - (reply: 5)
425. The same CT with different Quantity, why? - (reply: 8)
426. Adding tag using overlapping PCR - (reply: 2)
427. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
428. Design primer from incomplete sequence... - (reply: 2)
429. Long term storage for RNA extracted from pancreas - (reply: 3)
430. How to perform colony PCR - (reply: 1)
431. qPCR from HEK cells transfected with Plasmid - (reply: 4)
432. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
433. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
434. Negative Control Looks Like the Rest - (reply: 11)
435. Why RNase H treatment after cDNA synthesis - (reply: 2)
436. RNA isolation from Sesame seed - (reply: 3)
437. qPCR with Genomic DNA - (reply: 1)
438. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
439. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
440. Homogenize pancreas for RNA extraction - (reply: 3)
441. Point mutagenesis..... - (reply: 6)
442. No bands in PCR after DNase treatment - (reply: 4)
443. Overlap PCR problem - (reply: 5)
444. PCR product purification - (reply: 4)
445. Overlapping sequence PCR primers - (reply: 1)
446. acceptable melt curve? - (reply: 1)
447. Calibrator sample - (reply: 4)
448. Ct value very high. What can I do to improve this ct? - (reply: 4)
449. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
450. Amount of RNA (ng) for good ct values. - (reply: 3)