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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
421. Nested PCR - (reply: 2)
422. random primers or oligodT - (reply: 4)
423. Confused about normalization - (reply: 2)
424. To design or use published primers? - (reply: 4)
425. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
426. PCR primer usage (Clonning & cDNA) - (reply: 2)
427. Taq polymerase - (reply: 7)
428. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
429. Which High-Fidelity polymerase is better? - (reply: 2)
430. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
431. reference gene for qPCR in E.coli - (reply: 2)
432. Problem amplifying viral gene - (reply: 5)
433. The same CT with different Quantity, why? - (reply: 8)
434. Adding tag using overlapping PCR - (reply: 2)
435. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
436. Design primer from incomplete sequence... - (reply: 2)
437. Long term storage for RNA extracted from pancreas - (reply: 3)
438. How to perform colony PCR - (reply: 1)
439. qPCR from HEK cells transfected with Plasmid - (reply: 4)
440. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
441. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
442. Negative Control Looks Like the Rest - (reply: 11)
443. Why RNase H treatment after cDNA synthesis - (reply: 2)
444. RNA isolation from Sesame seed - (reply: 3)
445. qPCR with Genomic DNA - (reply: 1)
446. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
447. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
448. Homogenize pancreas for RNA extraction - (reply: 3)
449. Point mutagenesis..... - (reply: 6)
450. No bands in PCR after DNase treatment - (reply: 4)