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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
421. Confused about normalization - (reply: 2)
422. To design or use published primers? - (reply: 4)
423. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
424. PCR primer usage (Clonning & cDNA) - (reply: 2)
425. Taq polymerase - (reply: 7)
426. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
427. Which High-Fidelity polymerase is better? - (reply: 2)
428. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
429. reference gene for qPCR in E.coli - (reply: 2)
430. Problem amplifying viral gene - (reply: 5)
431. The same CT with different Quantity, why? - (reply: 8)
432. Adding tag using overlapping PCR - (reply: 2)
433. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
434. Design primer from incomplete sequence... - (reply: 2)
435. Long term storage for RNA extracted from pancreas - (reply: 3)
436. How to perform colony PCR - (reply: 1)
437. qPCR from HEK cells transfected with Plasmid - (reply: 4)
438. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
439. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
440. Negative Control Looks Like the Rest - (reply: 11)
441. Why RNase H treatment after cDNA synthesis - (reply: 2)
442. RNA isolation from Sesame seed - (reply: 3)
443. qPCR with Genomic DNA - (reply: 1)
444. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
445. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
446. Homogenize pancreas for RNA extraction - (reply: 3)
447. Point mutagenesis..... - (reply: 6)
448. No bands in PCR after DNase treatment - (reply: 4)
449. Overlap PCR problem - (reply: 5)
450. PCR product purification - (reply: 4)