Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
421. Adding tag using overlapping PCR - (reply: 2)
422. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
423. Design primer from incomplete sequence... - (reply: 2)
424. Long term storage for RNA extracted from pancreas - (reply: 3)
425. How to perform colony PCR - (reply: 1)
426. qPCR from HEK cells transfected with Plasmid - (reply: 4)
427. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
428. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
429. Negative Control Looks Like the Rest - (reply: 11)
430. Why RNase H treatment after cDNA synthesis - (reply: 2)
431. RNA isolation from Sesame seed - (reply: 3)
432. qPCR with Genomic DNA - (reply: 1)
433. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
434. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
435. Homogenize pancreas for RNA extraction - (reply: 3)
436. Point mutagenesis..... - (reply: 6)
437. No bands in PCR after DNase treatment - (reply: 4)
438. Overlap PCR problem - (reply: 5)
439. PCR product purification - (reply: 4)
440. Overlapping sequence PCR primers - (reply: 1)
441. acceptable melt curve? - (reply: 1)
442. Calibrator sample - (reply: 4)
443. Ct value very high. What can I do to improve this ct? - (reply: 4)
444. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
445. Amount of RNA (ng) for good ct values. - (reply: 3)
446. Normalizing qPCR data - (reply: 1)
447. doing PCR using PCR product , help plz !! - (reply: 5)
448. High GC content RT-qPCR - please help - (reply: 3)
449. Electrophoresis problem , please help pals ! - (reply: 8)
450. Calculating Standard Errors and Normalising to Transgene - (reply: 1)