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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
421. PCR amplified product size - (reply: 5)
422. Opinion: What fold change is actually considered meaningful - (reply: 2)
423. Buffers RNase decontamination - (reply: 1)
424. the storage time for primers - (reply: 9)
425. Annealing temperature for PCR - (reply: 8)
426. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
427. Nested PCR - (reply: 2)
428. random primers or oligodT - (reply: 4)
429. Confused about normalization - (reply: 2)
430. To design or use published primers? - (reply: 4)
431. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
432. PCR primer usage (Clonning & cDNA) - (reply: 2)
433. Taq polymerase - (reply: 7)
434. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
435. Which High-Fidelity polymerase is better? - (reply: 2)
436. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
437. reference gene for qPCR in E.coli - (reply: 2)
438. Problem amplifying viral gene - (reply: 5)
439. The same CT with different Quantity, why? - (reply: 8)
440. Adding tag using overlapping PCR - (reply: 2)
441. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
442. Design primer from incomplete sequence... - (reply: 2)
443. Long term storage for RNA extracted from pancreas - (reply: 3)
444. How to perform colony PCR - (reply: 1)
445. qPCR from HEK cells transfected with Plasmid - (reply: 4)
446. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
447. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
448. Negative Control Looks Like the Rest - (reply: 11)
449. Why RNase H treatment after cDNA synthesis - (reply: 2)
450. RNA isolation from Sesame seed - (reply: 3)