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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
421. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
422. Which High-Fidelity polymerase is better? - (reply: 2)
423. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
424. reference gene for qPCR in E.coli - (reply: 2)
425. Problem amplifying viral gene - (reply: 5)
426. The same CT with different Quantity, why? - (reply: 8)
427. Adding tag using overlapping PCR - (reply: 2)
428. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
429. Design primer from incomplete sequence... - (reply: 2)
430. Long term storage for RNA extracted from pancreas - (reply: 3)
431. How to perform colony PCR - (reply: 1)
432. qPCR from HEK cells transfected with Plasmid - (reply: 4)
433. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
434. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
435. Negative Control Looks Like the Rest - (reply: 11)
436. Why RNase H treatment after cDNA synthesis - (reply: 2)
437. RNA isolation from Sesame seed - (reply: 3)
438. qPCR with Genomic DNA - (reply: 1)
439. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
440. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
441. Homogenize pancreas for RNA extraction - (reply: 3)
442. Point mutagenesis..... - (reply: 6)
443. No bands in PCR after DNase treatment - (reply: 4)
444. Overlap PCR problem - (reply: 5)
445. PCR product purification - (reply: 4)
446. Overlapping sequence PCR primers - (reply: 1)
447. acceptable melt curve? - (reply: 1)
448. Calibrator sample - (reply: 4)
449. Ct value very high. What can I do to improve this ct? - (reply: 4)
450. how to make a working solution of a primers for pcr reaction?? - (reply: 4)