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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
421. Difference in Ct value of replicates - (reply: 1)
422. TDNA Genotyping PCR program - (reply: 1)
423. Shift in peaks at dissociation curve - (reply: 4)
424. High resolution melting analysis-SNP genotyping. - (reply: 1)
425. PCR - (reply: 2)
426. nonspecific band at 50bp in PCR - (reply: 9)
427. cDNA sequencing - (reply: 2)
428. Maximum PCR product Tm - (reply: 6)
429. QuikChange with Different plasmids - (reply: 1)
430. Good melting curve but non-specific products appeared - (reply: 2)
431. Long Non-Specific PCR Products - (reply: 5)
432. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
433. 5'RACE of RNA Pol3 transcripts? - (reply: 3)
434. fold change (how to represent it) - (reply: 1)
435. PCR - consistent false positive results - (reply: 6)
436. geNorm alternative for qPCR housekeeping genes - (reply: 1)
437. PCR no amplification - (reply: 15)
438. positive NTC in real time reaction - (reply: 12)
439. Upscaling cDNA synthesis using SuperScript™ III Reverse Transcriptase - (reply: 4)
440. How to quantify virus by PCR - (reply: 12)
441. qPCR contamination problem - (reply: 5)
442. Error rate of commecial reverse transcriptase - (reply: 1)
443. Why do i get DNA of low concentrate after purification with kit - (reply: 9)
444. low A260/230 problem - (reply: 2)
445. What is a use of ΔCt and ΔΔCt in qRT PCR? - (reply: 4)
446. Pcr product size determination for a fusion gene - (reply: 8)
447. open ABI 7500 documents in Macbook - (reply: 1)
448. Primer Annealing Temperatures - (reply: 24)
449. Molecular beacons problem - (reply: 6)
450. RT-qPCR plate set up NoRT controls - (reply: 1)