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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
421. RNA extraction - (reply: 3)
422. Normalization of Ct of interest to a reference Ct - (reply: 3)
423. urgent: need help with qPCR statistics - (reply: 2)
424. A question for Reverse transcription experts - (reply: 5)
425. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
426. PCR amplification with new restriction sites troubleshooting - (reply: 2)
427. using cell line to generate standard curve - (reply: 6)
428. PCR DNA Concentration - (reply: 1)
429. 3' RACE creates too big band doesn't leave well - (reply: 1)
430. RT-PCR product- no band - (reply: 4)
431. Normalization factor - (reply: 3)
432. Understanding RACE PCR - (reply: 1)
433. SYBR Select Master Mix for CFX from Invitrogen - Storage - (reply: 4)
434. Dye for qPCR - (reply: 3)
435. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
436. Wierd Bands after PCR....Confused - (reply: 9)
437. PCR with Plasmid recovered from filter paper - (reply: 6)
438. PCR amplified product size - (reply: 5)
439. Opinion: What fold change is actually considered meaningful - (reply: 2)
440. Buffers RNase decontamination - (reply: 1)
441. the storage time for primers - (reply: 9)
442. Annealing temperature for PCR - (reply: 8)
443. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
444. Nested PCR - (reply: 2)
445. random primers or oligodT - (reply: 4)
446. Confused about normalization - (reply: 2)
447. To design or use published primers? - (reply: 4)
448. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
449. PCR primer usage (Clonning & cDNA) - (reply: 2)
450. Taq polymerase - (reply: 7)