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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
421. Annealing temperature for PCR - (reply: 8)
422. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
423. Nested PCR - (reply: 2)
424. random primers or oligodT - (reply: 4)
425. Confused about normalization - (reply: 2)
426. To design or use published primers? - (reply: 4)
427. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
428. PCR primer usage (Clonning & cDNA) - (reply: 2)
429. Taq polymerase - (reply: 7)
430. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
431. Which High-Fidelity polymerase is better? - (reply: 2)
432. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
433. reference gene for qPCR in E.coli - (reply: 2)
434. Problem amplifying viral gene - (reply: 5)
435. The same CT with different Quantity, why? - (reply: 8)
436. Adding tag using overlapping PCR - (reply: 2)
437. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
438. Design primer from incomplete sequence... - (reply: 2)
439. Long term storage for RNA extracted from pancreas - (reply: 3)
440. How to perform colony PCR - (reply: 1)
441. qPCR from HEK cells transfected with Plasmid - (reply: 4)
442. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
443. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
444. Negative Control Looks Like the Rest - (reply: 11)
445. Why RNase H treatment after cDNA synthesis - (reply: 2)
446. RNA isolation from Sesame seed - (reply: 3)
447. qPCR with Genomic DNA - (reply: 1)
448. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
449. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
450. Homogenize pancreas for RNA extraction - (reply: 3)