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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
421. random primers or oligodT - (reply: 4)
422. Confused about normalization - (reply: 2)
423. To design or use published primers? - (reply: 4)
424. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
425. PCR primer usage (Clonning & cDNA) - (reply: 2)
426. Taq polymerase - (reply: 7)
427. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
428. Which High-Fidelity polymerase is better? - (reply: 2)
429. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
430. reference gene for qPCR in E.coli - (reply: 2)
431. Problem amplifying viral gene - (reply: 5)
432. The same CT with different Quantity, why? - (reply: 8)
433. Adding tag using overlapping PCR - (reply: 2)
434. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
435. Design primer from incomplete sequence... - (reply: 2)
436. Long term storage for RNA extracted from pancreas - (reply: 3)
437. How to perform colony PCR - (reply: 1)
438. qPCR from HEK cells transfected with Plasmid - (reply: 4)
439. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
440. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
441. Negative Control Looks Like the Rest - (reply: 11)
442. Why RNase H treatment after cDNA synthesis - (reply: 2)
443. RNA isolation from Sesame seed - (reply: 3)
444. qPCR with Genomic DNA - (reply: 1)
445. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
446. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
447. Homogenize pancreas for RNA extraction - (reply: 3)
448. Point mutagenesis..... - (reply: 6)
449. No bands in PCR after DNase treatment - (reply: 4)
450. Overlap PCR problem - (reply: 5)