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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1021. Ct's of goi higher and lower than ct's hkg - (reply: 1)
1022. Effect of collagenase on qpcr results - (reply: 1)
1023. RNA Isolation - (reply: 3)
1024. mRNA Search for RT-PCR (U to T) - (reply: 2)
1025. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
1026. PCR triplicates versus one reaction - differences? (reply: 17)
1027. cloning of race products - (reply: 1)
1028. Single or double-stranded cDNA? - (reply: 2)
1029. Comparing SYBR and probe-based Ct values - Is it valid? (reply: 2)
1030. qPCR inhibition - (reply: 1)
1031. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
1032. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
1033. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
1034. Primers and self and hetero dimers - (reply: 1)
1035. Really weird amplification curves - (reply: 1)
1036. Primers for qPCR - (reply: 3)
1037. copy number calculation from cDNA - (reply: 1)
1038. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
1039. RT-PCR primer - (reply: 3)
1040. CT value>25 with GAPDH in 50-fold dilution! - (reply: 3)
1041. PCR problem - basic PCR for plasmid amplification (reply: 2)
1042. can RNA amplify in two-step qPCR? - (reply: 2)
1043. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
1044. Nested LA PCR - Any success? (reply: 3)
1045. Unpredictablity of PCR product - (reply: 5)
1046. total cDNA amplification by PCR - (reply: 2)
1047. Detection limit of a conventional PCR - (reply: 2)
1048. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
1049. RNAi showing upregulation via QPCR - Help needed (reply: 3)
1050. DNA detection limit of the PCR - Calculating detection limits (reply: 4)