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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1021. cDNA synthesis - (reply: 6)
1022. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
1023. Primer seq - (reply: 2)
1024. Selection of housekeeping gene in Mycobacterium - (reply: 1)
1025. Do I need to pool the base line samples together - (reply: 2)
1026. PCR product size - (reply: 6)
1027. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
1028. Few basic questions about RNA - (reply: 3)
1029. oligo dt and random primer - (reply: 3)
1030. Ct's of goi higher and lower than ct's hkg - (reply: 1)
1031. Effect of collagenase on qpcr results - (reply: 1)
1032. RNA Isolation - (reply: 3)
1033. mRNA Search for RT-PCR (U to T) - (reply: 2)
1034. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
1035. PCR triplicates versus one reaction - differences? (reply: 17)
1036. cloning of race products - (reply: 1)
1037. Single or double-stranded cDNA? - (reply: 2)
1038. Comparing SYBR and probe-based Ct values - Is it valid? (reply: 2)
1039. qPCR inhibition - (reply: 1)
1040. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
1041. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
1042. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
1043. Primers and self and hetero dimers - (reply: 1)
1044. Really weird amplification curves - (reply: 1)
1045. Primers for qPCR - (reply: 3)
1046. copy number calculation from cDNA - (reply: 1)
1047. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
1048. RT-PCR primer - (reply: 3)
1049. CT value>25 with GAPDH in 50-fold dilution! - (reply: 3)
1050. PCR problem - basic PCR for plasmid amplification (reply: 2)