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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1021. Primers and self and hetero dimers - (reply: 1)
1022. Really weird amplification curves - (reply: 1)
1023. Primers for qPCR - (reply: 3)
1024. copy number calculation from cDNA - (reply: 1)
1025. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
1026. RT-PCR primer - (reply: 3)
1027. CT value>25 with GAPDH in 50-fold dilution! - (reply: 3)
1028. PCR problem - basic PCR for plasmid amplification (reply: 2)
1029. can RNA amplify in two-step qPCR? - (reply: 2)
1030. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
1031. Nested LA PCR - Any success? (reply: 3)
1032. Unpredictablity of PCR product - (reply: 5)
1033. total cDNA amplification by PCR - (reply: 2)
1034. Detection limit of a conventional PCR - (reply: 2)
1035. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
1036. RNAi showing upregulation via QPCR - Help needed (reply: 3)
1037. DNA detection limit of the PCR - Calculating detection limits (reply: 4)
1038. cDNA synthesis - (reply: 3)
1039. 2 rounds PCR got problem - (reply: 2)
1040. PCR help minus strand - Primer design (reply: 1)
1041. cDNA contamination, pls help! - (reply: 1)
1042. Smear in long distance PCR - (reply: 39)
1043. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
1044. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
1045. can contaminating gDNA explain this? - (reply: 1)
1046. TOUCH DOWN PCR - (reply: 2)
1047. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
1048. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
1049. PCR stopped working - After changing buffer (reply: 5)
1050. how to determine the amount of cDNA for qPCR - (reply: 5)