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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1021. TOUCH DOWN PCR - (reply: 2)
1022. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
1023. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
1024. PCR stopped working - After changing buffer (reply: 5)
1025. how to determine the amount of cDNA for qPCR - (reply: 5)
1026. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)
1027. Standardization of HRM for methylation analysis - (reply: 4)
1028. PCR protocol questions! - (reply: 2)
1029. standard curve for qPCR - (reply: 3)
1030. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
1031. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
1032. DNA amount calculation for PCR - (reply: 14)
1033. ChIP primer design - (reply: 1)
1034. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
1035. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
1036. weird QPCR curves - (reply: 2)
1037. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
1038. Questions regarding RT-PCR optimization - (reply: 2)
1039. Problems with semi-quatitative PCR - (reply: 1)
1040. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
1041. Long-amplicon QPCR of mtDNA - (reply: 5)
1042. PCR reaction calculation - (reply: 2)
1043. Qiagen rotorgene syber green kit reaction volume - (reply: 1)
1044. missing nt at the 5' end - site mutagensis using NEB kit (reply: 1)
1045. no increase in fluorescence in my real time PCR - (reply: 3)
1046. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
1047. Roche second derivative maximum - can be treated according to Pfaffl? (reply: 1)
1048. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
1049. Pooled RNA from healthy controls - delta-delta Ct method (reply: 1)
1050. Probe Dilution Problem = 100uM to 5ng/ul? + Final Volume for FISH - Probe dilution and FISH - Advise on volume for FISH hibridization buff (reply: 2)