Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1021. 2 rounds PCR got problem - (reply: 2)
1022. PCR help minus strand - Primer design (reply: 1)
1023. cDNA contamination, pls help! - (reply: 1)
1024. Smear in long distance PCR - (reply: 39)
1025. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
1026. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
1027. can contaminating gDNA explain this? - (reply: 1)
1028. TOUCH DOWN PCR - (reply: 2)
1029. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
1030. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
1031. PCR stopped working - After changing buffer (reply: 5)
1032. how to determine the amount of cDNA for qPCR - (reply: 5)
1033. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)
1034. Standardization of HRM for methylation analysis - (reply: 4)
1035. PCR protocol questions! - (reply: 2)
1036. standard curve for qPCR - (reply: 3)
1037. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
1038. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
1039. DNA amount calculation for PCR - (reply: 14)
1040. ChIP primer design - (reply: 1)
1041. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
1042. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
1043. weird QPCR curves - (reply: 2)
1044. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
1045. Questions regarding RT-PCR optimization - (reply: 2)
1046. Problems with semi-quatitative PCR - (reply: 1)
1047. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
1048. Long-amplicon QPCR of mtDNA - (reply: 5)
1049. PCR reaction calculation - (reply: 2)
1050. Qiagen rotorgene syber green kit reaction volume - (reply: 1)