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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1021. RT-PCR - (reply: 9)
1022. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
1023. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
1024. cDNA synthesis - (reply: 6)
1025. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
1026. Primer seq - (reply: 2)
1027. Selection of housekeeping gene in Mycobacterium - (reply: 1)
1028. Do I need to pool the base line samples together - (reply: 2)
1029. PCR product size - (reply: 6)
1030. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
1031. Few basic questions about RNA - (reply: 3)
1032. oligo dt and random primer - (reply: 3)
1033. Ct's of goi higher and lower than ct's hkg - (reply: 1)
1034. Effect of collagenase on qpcr results - (reply: 1)
1035. RNA Isolation - (reply: 3)
1036. mRNA Search for RT-PCR (U to T) - (reply: 2)
1037. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
1038. PCR triplicates versus one reaction - differences? (reply: 17)
1039. cloning of race products - (reply: 1)
1040. Single or double-stranded cDNA? - (reply: 2)
1041. Comparing SYBR and probe-based Ct values - Is it valid? (reply: 2)
1042. qPCR inhibition - (reply: 1)
1043. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
1044. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
1045. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
1046. Primers and self and hetero dimers - (reply: 1)
1047. Really weird amplification curves - (reply: 1)
1048. Primers for qPCR - (reply: 3)
1049. copy number calculation from cDNA - (reply: 1)
1050. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)