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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1021. Unpredictablity of PCR product - (reply: 5)
1022. total cDNA amplification by PCR - (reply: 2)
1023. Detection limit of a conventional PCR - (reply: 2)
1024. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
1025. RNAi showing upregulation via QPCR - Help needed (reply: 3)
1026. DNA detection limit of the PCR - Calculating detection limits (reply: 4)
1027. cDNA synthesis - (reply: 3)
1028. 2 rounds PCR got problem - (reply: 2)
1029. PCR help minus strand - Primer design (reply: 1)
1030. cDNA contamination, pls help! - (reply: 1)
1031. Smear in long distance PCR - (reply: 39)
1032. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
1033. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
1034. can contaminating gDNA explain this? - (reply: 1)
1035. TOUCH DOWN PCR - (reply: 2)
1036. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
1037. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
1038. PCR stopped working - After changing buffer (reply: 5)
1039. how to determine the amount of cDNA for qPCR - (reply: 5)
1040. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)
1041. Standardization of HRM for methylation analysis - (reply: 4)
1042. PCR protocol questions! - (reply: 2)
1043. standard curve for qPCR - (reply: 3)
1044. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
1045. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
1046. DNA amount calculation for PCR - (reply: 14)
1047. ChIP primer design - (reply: 1)
1048. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
1049. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
1050. weird QPCR curves - (reply: 2)