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1021. silly control question - No RT controls (reply: 2)
1022. Long Primers for PCR - (reply: 2)
1023. Data Analysis for Real-time PCR - (reply: 2)
1024. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
1025. argh more pcr headaches! - (reply: 6)
1026. Annealing temperature - (reply: 3)
1027. Replicate inconsistencies in qPCR - (reply: 6)
1028. With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
1029. Chlamydia PCR cycling conditions - (reply: 1)
1030. On-unit screen or PC…which is better? - (reply: 12)
1031. Primers going "loopy" - (reply: 2)
1032. Biotinylated! - brief explanaion (reply: 1)
1033. what do i say?! - (reply: 2)
1034. Smearing on gel in PCR products - (reply: 5)
1035. 260/230 ratio: how low is good for running PCRs? - (reply: 2)
1036. introducing alanine mutations - (reply: 1)
1037. Hematin - calculation (reply: 1)
1038. How to generate sticky ends? - (reply: 3)
1039. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
1040. PROBLEM WITH MELTING CURVES - i realy need help (reply: 2)
1041. Relative RT-PCR, multiplate and calibration - (reply: 4)
1042. Trying to measure small levels of mRNA - (reply: 3)
1043. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
1044. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
1045. Relative RT-PCR - (reply: 2)
1046. Relative real time RT PCR - (reply: 2)
1047. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
1048. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
1049. IQ5 and sybr/FAM - (reply: 3)
1050. a simple primer problem - (reply: 5)
1022. Long Primers for PCR - (reply: 2)
1023. Data Analysis for Real-time PCR - (reply: 2)
1024. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
1025. argh more pcr headaches! - (reply: 6)
1026. Annealing temperature - (reply: 3)
1027. Replicate inconsistencies in qPCR - (reply: 6)
1028. With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
1029. Chlamydia PCR cycling conditions - (reply: 1)
1030. On-unit screen or PC…which is better? - (reply: 12)
1031. Primers going "loopy" - (reply: 2)
1032. Biotinylated! - brief explanaion (reply: 1)
1033. what do i say?! - (reply: 2)
1034. Smearing on gel in PCR products - (reply: 5)
1035. 260/230 ratio: how low is good for running PCRs? - (reply: 2)
1036. introducing alanine mutations - (reply: 1)
1037. Hematin - calculation (reply: 1)
1038. How to generate sticky ends? - (reply: 3)
1039. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
1040. PROBLEM WITH MELTING CURVES - i realy need help (reply: 2)
1041. Relative RT-PCR, multiplate and calibration - (reply: 4)
1042. Trying to measure small levels of mRNA - (reply: 3)
1043. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
1044. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
1045. Relative RT-PCR - (reply: 2)
1046. Relative real time RT PCR - (reply: 2)
1047. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
1048. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
1049. IQ5 and sybr/FAM - (reply: 3)
1050. a simple primer problem - (reply: 5)