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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1021. RT-PCR primer - (reply: 3)
1022. CT value>25 with GAPDH in 50-fold dilution! - (reply: 3)
1023. PCR problem - basic PCR for plasmid amplification (reply: 2)
1024. can RNA amplify in two-step qPCR? - (reply: 2)
1025. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
1026. Nested LA PCR - Any success? (reply: 3)
1027. Unpredictablity of PCR product - (reply: 5)
1028. total cDNA amplification by PCR - (reply: 2)
1029. Detection limit of a conventional PCR - (reply: 2)
1030. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
1031. RNAi showing upregulation via QPCR - Help needed (reply: 3)
1032. DNA detection limit of the PCR - Calculating detection limits (reply: 4)
1033. cDNA synthesis - (reply: 3)
1034. 2 rounds PCR got problem - (reply: 2)
1035. PCR help minus strand - Primer design (reply: 1)
1036. cDNA contamination, pls help! - (reply: 1)
1037. Smear in long distance PCR - (reply: 39)
1038. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
1039. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
1040. can contaminating gDNA explain this? - (reply: 1)
1041. TOUCH DOWN PCR - (reply: 2)
1042. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
1043. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
1044. PCR stopped working - After changing buffer (reply: 5)
1045. how to determine the amount of cDNA for qPCR - (reply: 5)
1046. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)
1047. Standardization of HRM for methylation analysis - (reply: 4)
1048. PCR protocol questions! - (reply: 2)
1049. standard curve for qPCR - (reply: 3)
1050. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)