Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1021. no increase in fluorescence in my real time PCR - (reply: 3)
1022. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
1023. Roche second derivative maximum - can be treated according to Pfaffl? (reply: 1)
1024. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
1025. Pooled RNA from healthy controls - delta-delta Ct method (reply: 1)
1026. Probe Dilution Problem = 100uM to 5ng/ul? + Final Volume for FISH - Probe dilution and FISH - Advise on volume for FISH hibridization buff (reply: 2)
1027. PCR, using gel extracted band as a template - (reply: 4)
1028. Question of nucleic acid extraction - (reply: 1)
1029. Multiplex problems - (reply: 6)
1030. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
1031. Q-PCR and RT-PCR - (reply: 3)
1032. inter-run calibration - (reply: 4)
1033. Delta Delta Ct method for multiple reference genes? - (reply: 1)
1034. FAM and VIC labelling - (reply: 2)
1035. Lost cause?? - I made a silly (reply: 4)
1036. how to use delta-delta Ct method? - when no expression in healthy controls! (reply: 2)
1037. confusing PCR - (reply: 1)
1038. difference between PCR primers and sequencing primers - (reply: 1)
1039. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
1040. making gel electrophoresis - (reply: 1)
1041. housekeeping genes in PCR - (reply: 1)
1042. Question about RealTime PCR Primer Design - (reply: 1)
1043. Primers stopped working!? - PCR Primers (reply: 6)
1044. NFK beta primers - (reply: 4)
1045. final PCR product - need clarifications (reply: 1)
1046. Trying to amplify the 3' end of Prolyl endopeptidse gene from cDNA.. need su - Hi, I'm trying to sequence a gene from the cDNA of an insect (reply: 2)
1047. Can I use Hot Start Taq polimerase for any target? - (reply: 6)
1048. Ct value and interpretation - (reply: 3)
1049. 16S rDNA - Bacteria identification (reply: 1)
1050. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)