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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1021. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
1022. RNAi showing upregulation via QPCR - Help needed (reply: 3)
1023. DNA detection limit of the PCR - Calculating detection limits (reply: 4)
1024. cDNA synthesis - (reply: 3)
1025. 2 rounds PCR got problem - (reply: 2)
1026. PCR help minus strand - Primer design (reply: 1)
1027. cDNA contamination, pls help! - (reply: 1)
1028. Smear in long distance PCR - (reply: 39)
1029. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
1030. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
1031. can contaminating gDNA explain this? - (reply: 1)
1032. TOUCH DOWN PCR - (reply: 2)
1033. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
1034. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
1035. PCR stopped working - After changing buffer (reply: 5)
1036. how to determine the amount of cDNA for qPCR - (reply: 5)
1037. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)
1038. Standardization of HRM for methylation analysis - (reply: 4)
1039. PCR protocol questions! - (reply: 2)
1040. standard curve for qPCR - (reply: 3)
1041. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
1042. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
1043. DNA amount calculation for PCR - (reply: 14)
1044. ChIP primer design - (reply: 1)
1045. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
1046. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
1047. weird QPCR curves - (reply: 2)
1048. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
1049. Questions regarding RT-PCR optimization - (reply: 2)
1050. Problems with semi-quatitative PCR - (reply: 1)