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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1021. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
1022. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
1023. can contaminating gDNA explain this? - (reply: 1)
1024. TOUCH DOWN PCR - (reply: 2)
1025. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
1026. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
1027. PCR stopped working - After changing buffer (reply: 5)
1028. how to determine the amount of cDNA for qPCR - (reply: 5)
1029. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)
1030. Standardization of HRM for methylation analysis - (reply: 4)
1031. PCR protocol questions! - (reply: 2)
1032. standard curve for qPCR - (reply: 3)
1033. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
1034. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
1035. DNA amount calculation for PCR - (reply: 14)
1036. ChIP primer design - (reply: 1)
1037. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
1038. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
1039. weird QPCR curves - (reply: 2)
1040. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
1041. Questions regarding RT-PCR optimization - (reply: 2)
1042. Problems with semi-quatitative PCR - (reply: 1)
1043. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
1044. Long-amplicon QPCR of mtDNA - (reply: 5)
1045. PCR reaction calculation - (reply: 2)
1046. Qiagen rotorgene syber green kit reaction volume - (reply: 1)
1047. missing nt at the 5' end - site mutagensis using NEB kit (reply: 1)
1048. no increase in fluorescence in my real time PCR - (reply: 3)
1049. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
1050. Roche second derivative maximum - can be treated according to Pfaffl? (reply: 1)