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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1681. qPCR primer design - (reply: 5)
1682. Re-PCR problem - (reply: 3)
1683. PCR and gel purification problem - did I screw this up (reply: 4)
1684. touchdown pcr - (reply: 6)
1685. non specific pcr products - (reply: 4)
1686. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
1687. template concentration qPCR - (reply: 1)
1688. Anyone with experiences in 2 step PCR - (reply: 1)
1689. qRT-PCR Problems - (reply: 1)
1690. PCR works on lab strain but not patient sample - HIV related (reply: 4)
1691. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
1692. Genomic DNA copy number qPCR - Help with control (reply: 8)
1693. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
1694. re-amplification of a PCR product - is it recommended? (reply: 7)
1695. PCR machine with accurate temperature control? - (reply: 1)
1696. qRT-PCR with DMSO - (reply: 2)
1697. Gene-specific RT-PCR - (reply: 2)
1698. Gain! - (reply: 4)
1699. selection of internal control gene - (reply: 2)
1700. 2-ΔΔCT interpretation of gene copy number - What does a 2-ΔΔCT value of 1.84 or 0.7 mean? (reply: 3)
1701. primer design - primer design question (reply: 2)
1702. Protocol for 16S rDNA real time PCR - (reply: 2)
1703. qPCR - Standard curves (reply: 3)
1704. cDNA spectrophotometric quantification - (reply: 8)
1705. Standard curve standardization - variation between runs (reply: 4)
1706. How to recognize primer-dimers? - from a melting curve??... (reply: 4)
1707. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
1708. real time curve shifted by ~10 cycles - higher Cts than I used to (reply: 2)
1709. low gc primers - help with pcr using low gc primers (reply: 6)
1710. Exon-Exon Junction ? - (reply: 3)