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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1681. RT-PCR - problems with TaqMan PCR (reply: 2)
1682. qRT-PCR calculations - Averaging across biological replicates (reply: 2)
1683. Two annealing temps? - (reply: 4)
1684. Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
1685. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
1686. Small product amplification problems - (reply: 4)
1687. ROX and Rotorgene - using a mix with reference dye in Rotorgene (reply: 2)
1688. Freezing primers+SYBR green - (reply: 2)
1689. Tagged Primers - (reply: 5)
1690. no pcr amplification product - (reply: 9)
1691. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
1692. less steep curve and high Ct value! - (reply: 2)
1693. Question about site directed mutagenesis - (reply: 1)
1694. Amplfication of fungal DNA - (reply: 8)
1695. PCR problem - (reply: 4)
1696. mRNA level in transgene cell line or knocking down cell line - (reply: 3)
1697. differently behavioring replicates in real-time PCR - (reply: 1)
1698. Multiplex PCR - (reply: 1)
1699. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
1700. optimal template dna concentrations - (reply: 1)
1701. reconstitution of primers - (reply: 2)
1702. Mysterious PCR Contamination - (reply: 13)
1703. RT-PCR Help - Protocol provided (reply: 4)
1704. Endogenous control in bacterial RT-qPCR - (reply: 4)
1705. Mutagenesis PCR and undesired amplification - (reply: 2)
1706. PCR-how to set 'Extension: ramping from 55 to 72 for 5min' - (reply: 4)
1707. primer dimer - (reply: 2)
1708. Genotyping conditioning-where to start? - (reply: 1)
1709. Marker migrates slower depending where is loaded - (reply: 2)
1710. PCR on degraded templates - (reply: 2)