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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1681. primer dimer - (reply: 2)
1682. Genotyping conditioning-where to start? - (reply: 1)
1683. Marker migrates slower depending where is loaded - (reply: 2)
1684. PCR on degraded templates - (reply: 2)
1685. PCR help needed - (reply: 3)
1686. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
1687. Tm difference - (reply: 2)
1688. Genotyping - PCR not working (reply: 6)
1689. RT qPCR Newbie, Weird Results - (reply: 1)
1690. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
1691. light bands, dark smear, dark dimers - (reply: 2)
1692. PCR, followed by sequencing... - why ?? (reply: 3)
1693. qPCR and varying cDNA concentrations - (reply: 1)
1694. PCR program - strange extension step (reply: 2)
1695. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
1696. Trouble with GAPDH housekeeping gene - (reply: 3)
1697. REAL TIME PCR 7005 v 2.01 : I need more reference samples! - How to add more than one reference sample to Real Time?? (reply: 1)
1698. qPCR lacks reproduceability - perfect runs, no reproduceability, no explaination, desperate... (reply: 5)
1699. Using BLAST to check primers - (reply: 17)
1700. qPCR and quantity of RNA - (reply: 2)
1701. pcr doesnt show any band for DNa walking - (reply: 8)
1702. How & where to prepare qPCR - (reply: 9)
1703. about qPCR plates sealing films - Axygen UC-500 vs. UC-200 (reply: 4)
1704. storing large amounts of PCR mastermix beforehand - question (reply: 3)
1705. questions about semi-quantitive PCR - weird result form semi-quantitive PCR (reply: 2)
1706. Effects of more cDNA in PCR??? - (reply: 1)
1707. PCR product disappears after restriction digestion - (reply: 4)
1708. Real Time PCR - excluding Ct - (reply: 2)
1709. PCR product looks less intense compared to others - should I increase the number of cycles? (reply: 4)
1710. DNA polymerases for PCR-sequencing - (reply: 11)