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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1681. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
1682. optimal template dna concentrations - (reply: 1)
1683. reconstitution of primers - (reply: 2)
1684. Mysterious PCR Contamination - (reply: 13)
1685. RT-PCR Help - Protocol provided (reply: 4)
1686. Endogenous control in bacterial RT-qPCR - (reply: 4)
1687. Mutagenesis PCR and undesired amplification - (reply: 2)
1688. PCR-how to set 'Extension: ramping from 55 to 72 for 5min' - (reply: 4)
1689. primer dimer - (reply: 2)
1690. Genotyping conditioning-where to start? - (reply: 1)
1691. Marker migrates slower depending where is loaded - (reply: 2)
1692. PCR on degraded templates - (reply: 2)
1693. PCR help needed - (reply: 3)
1694. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
1695. Tm difference - (reply: 2)
1696. Genotyping - PCR not working (reply: 6)
1697. RT qPCR Newbie, Weird Results - (reply: 1)
1698. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
1699. light bands, dark smear, dark dimers - (reply: 2)
1700. PCR, followed by sequencing... - why ?? (reply: 3)
1701. qPCR and varying cDNA concentrations - (reply: 1)
1702. PCR program - strange extension step (reply: 2)
1703. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
1704. Trouble with GAPDH housekeeping gene - (reply: 3)
1705. REAL TIME PCR 7005 v 2.01 : I need more reference samples! - How to add more than one reference sample to Real Time?? (reply: 1)
1706. qPCR lacks reproduceability - perfect runs, no reproduceability, no explaination, desperate... (reply: 5)
1707. Using BLAST to check primers - (reply: 17)
1708. qPCR and quantity of RNA - (reply: 2)
1709. pcr doesnt show any band for DNa walking - (reply: 8)
1710. How & where to prepare qPCR - (reply: 9)