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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1681. qRT-PCR Problems - (reply: 1)
1682. PCR works on lab strain but not patient sample - HIV related (reply: 4)
1683. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
1684. Genomic DNA copy number qPCR - Help with control (reply: 8)
1685. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
1686. re-amplification of a PCR product - is it recommended? (reply: 7)
1687. PCR machine with accurate temperature control? - (reply: 1)
1688. qRT-PCR with DMSO - (reply: 2)
1689. Gene-specific RT-PCR - (reply: 2)
1690. Gain! - (reply: 4)
1691. selection of internal control gene - (reply: 2)
1692. 2-ΔΔCT interpretation of gene copy number - What does a 2-ΔΔCT value of 1.84 or 0.7 mean? (reply: 3)
1693. primer design - primer design question (reply: 2)
1694. Protocol for 16S rDNA real time PCR - (reply: 2)
1695. qPCR - Standard curves (reply: 3)
1696. cDNA spectrophotometric quantification - (reply: 8)
1697. Standard curve standardization - variation between runs (reply: 4)
1698. How to recognize primer-dimers? - from a melting curve??... (reply: 4)
1699. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
1700. real time curve shifted by ~10 cycles - higher Cts than I used to (reply: 2)
1701. low gc primers - help with pcr using low gc primers (reply: 6)
1702. Exon-Exon Junction ? - (reply: 3)
1703. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
1704. Primers and annealing temperature - (reply: 2)
1705. Bacteria gene expression - (reply: 2)
1706. Copy number calculations in real time PCR - (reply: 2)
1707. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
1708. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
1709. RT-PCR Negative controls - (reply: 1)
1710. Problems with qRT-PCR - (reply: 3)