Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1681. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
1682. re-amplification of a PCR product - is it recommended? (reply: 7)
1683. PCR machine with accurate temperature control? - (reply: 1)
1684. qRT-PCR with DMSO - (reply: 2)
1685. Gene-specific RT-PCR - (reply: 2)
1686. Gain! - (reply: 4)
1687. selection of internal control gene - (reply: 2)
1688. 2-ΔΔCT interpretation of gene copy number - What does a 2-ΔΔCT value of 1.84 or 0.7 mean? (reply: 3)
1689. primer design - primer design question (reply: 2)
1690. Protocol for 16S rDNA real time PCR - (reply: 2)
1691. qPCR - Standard curves (reply: 3)
1692. cDNA spectrophotometric quantification - (reply: 8)
1693. Standard curve standardization - variation between runs (reply: 4)
1694. How to recognize primer-dimers? - from a melting curve??... (reply: 4)
1695. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
1696. real time curve shifted by ~10 cycles - higher Cts than I used to (reply: 2)
1697. low gc primers - help with pcr using low gc primers (reply: 6)
1698. Exon-Exon Junction ? - (reply: 3)
1699. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
1700. Primers and annealing temperature - (reply: 2)
1701. Bacteria gene expression - (reply: 2)
1702. Copy number calculations in real time PCR - (reply: 2)
1703. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
1704. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
1705. RT-PCR Negative controls - (reply: 1)
1706. Problems with qRT-PCR - (reply: 3)
1707. PCR with 60 bp primers - results in no product (reply: 6)
1708. Shrimp nuclease to eliminate genomic DNA - (reply: 7)
1709. How do you manually determine Ct value? - (reply: 1)
1710. Running gel after RT-PCR - (reply: 5)