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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1681. Mysterious PCR Contamination - (reply: 13)
1682. RT-PCR Help - Protocol provided (reply: 4)
1683. Endogenous control in bacterial RT-qPCR - (reply: 4)
1684. Mutagenesis PCR and undesired amplification - (reply: 2)
1685. PCR-how to set 'Extension: ramping from 55 to 72 for 5min' - (reply: 4)
1686. primer dimer - (reply: 2)
1687. Genotyping conditioning-where to start? - (reply: 1)
1688. Marker migrates slower depending where is loaded - (reply: 2)
1689. PCR on degraded templates - (reply: 2)
1690. PCR help needed - (reply: 3)
1691. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
1692. Tm difference - (reply: 2)
1693. Genotyping - PCR not working (reply: 6)
1694. RT qPCR Newbie, Weird Results - (reply: 1)
1695. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
1696. light bands, dark smear, dark dimers - (reply: 2)
1697. PCR, followed by sequencing... - why ?? (reply: 3)
1698. qPCR and varying cDNA concentrations - (reply: 1)
1699. PCR program - strange extension step (reply: 2)
1700. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
1701. Trouble with GAPDH housekeeping gene - (reply: 3)
1702. REAL TIME PCR 7005 v 2.01 : I need more reference samples! - How to add more than one reference sample to Real Time?? (reply: 1)
1703. qPCR lacks reproduceability - perfect runs, no reproduceability, no explaination, desperate... (reply: 5)
1704. Using BLAST to check primers - (reply: 17)
1705. qPCR and quantity of RNA - (reply: 2)
1706. pcr doesnt show any band for DNa walking - (reply: 8)
1707. How & where to prepare qPCR - (reply: 9)
1708. about qPCR plates sealing films - Axygen UC-500 vs. UC-200 (reply: 4)
1709. storing large amounts of PCR mastermix beforehand - question (reply: 3)
1710. questions about semi-quantitive PCR - weird result form semi-quantitive PCR (reply: 2)