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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1441. How to generate sticky ends? - (reply: 3)
1442. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
1443. PROBLEM WITH MELTING CURVES - i realy need help (reply: 2)
1444. Relative RT-PCR, multiplate and calibration - (reply: 4)
1445. Trying to measure small levels of mRNA - (reply: 3)
1446. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
1447. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
1448. Relative RT-PCR - (reply: 2)
1449. Relative real time RT PCR - (reply: 2)
1450. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
1451. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
1452. IQ5 and sybr/FAM - (reply: 3)
1453. a simple primer problem - (reply: 5)
1454. Failure to introduce mutations using Overlap PCR - (reply: 3)
1455. no or poor amplification - (reply: 1)
1456. Primers from 3'UTR - (reply: 2)
1457. Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
1458. Designing primers with ESTs - (reply: 2)
1459. Ct variation/Ct limit/rē values/efficiency errors - (reply: 2)
1460. Probe manufacturers, who makes best probes? - Where do you get your probes and are you happy with them? (reply: 3)
1461. Real Time vs Traditional PCR results - (reply: 1)
1462. The problems to identify mouse genotype with PCR - (reply: 1)
1463. non specific amplification - DNA amplification by PCR (reply: 1)
1464. RNA Extraction - Extraction of Yeast RNA from mice blood (reply: 1)
1465. Large Difference between Ct of 18s rRNA and Target Gene - (reply: 2)
1466. PCR has stopped working - (reply: 7)
1467. PCR negative control contamination - (reply: 8)
1468. Real-Time PCR - Gene expression (reply: 2)
1469. PCR efficiency in real timePCR - (reply: 5)
1470. Plate setup for qRT-PCR - Too many samples for one plate... (reply: 1)