Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1441. a simple primer problem - (reply: 5)
1442. Failure to introduce mutations using Overlap PCR - (reply: 3)
1443. no or poor amplification - (reply: 1)
1444. Primers from 3'UTR - (reply: 2)
1445. Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
1446. Designing primers with ESTs - (reply: 2)
1447. Ct variation/Ct limit/rē values/efficiency errors - (reply: 2)
1448. Probe manufacturers, who makes best probes? - Where do you get your probes and are you happy with them? (reply: 3)
1449. Real Time vs Traditional PCR results - (reply: 1)
1450. The problems to identify mouse genotype with PCR - (reply: 1)
1451. non specific amplification - DNA amplification by PCR (reply: 1)
1452. RNA Extraction - Extraction of Yeast RNA from mice blood (reply: 1)
1453. Large Difference between Ct of 18s rRNA and Target Gene - (reply: 2)
1454. PCR has stopped working - (reply: 7)
1455. PCR negative control contamination - (reply: 8)
1456. Real-Time PCR - Gene expression (reply: 2)
1457. PCR efficiency in real timePCR - (reply: 5)
1458. Plate setup for qRT-PCR - Too many samples for one plate... (reply: 1)
1459. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
1460. QPCR analysis with various # of samples from 1 patient? - (reply: 1)
1461. Drastic Decrease in PCR product yield - (reply: 2)
1462. Contamination? - (reply: 1)
1463. multiplexing taqman miRNA reverse transcription - (reply: 2)
1464. ligation problem - (reply: 3)
1465. Analyzing qPCR results - (reply: 1)
1466. resources on PCR principles & technique - (reply: 1)
1467. inconsistent results - easy 16S amplifcation is not so easy - (reply: 1)
1468. Problem cDNA synthesis/ 2 step RT - (reply: 6)
1469. High MW PCR band seen...help needed - (reply: 7)
1470. QPCR - Primer and Probe question - QPCR (reply: 2)