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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1441. Bad PCR, is it due to conditions, reagents, or lack of DNA template? - (reply: 1)
1442. Taqman real-time PCR - Taqman and contamination (reply: 4)
1443. silly control question - No RT controls (reply: 2)
1444. Long Primers for PCR - (reply: 2)
1445. Data Analysis for Real-time PCR - (reply: 2)
1446. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
1447. argh more pcr headaches! - (reply: 6)
1448. Annealing temperature - (reply: 3)
1449. Replicate inconsistencies in qPCR - (reply: 6)
1450. With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
1451. Chlamydia PCR cycling conditions - (reply: 1)
1452. On-unit screen or PC…which is better? - (reply: 12)
1453. Primers going "loopy" - (reply: 2)
1454. Biotinylated! - brief explanaion (reply: 2)
1455. what do i say?! - (reply: 2)
1456. Smearing on gel in PCR products - (reply: 5)
1457. 260/230 ratio: how low is good for running PCRs? - (reply: 2)
1458. introducing alanine mutations - (reply: 1)
1459. Hematin - calculation (reply: 1)
1460. How to generate sticky ends? - (reply: 3)
1461. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
1462. PROBLEM WITH MELTING CURVES - i realy need help (reply: 2)
1463. Relative RT-PCR, multiplate and calibration - (reply: 4)
1464. Trying to measure small levels of mRNA - (reply: 3)
1465. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
1466. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
1467. Relative RT-PCR - (reply: 2)
1468. Relative real time RT PCR - (reply: 2)
1469. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
1470. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)