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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1441. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
1442. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
1443. IQ5 and sybr/FAM - (reply: 3)
1444. a simple primer problem - (reply: 5)
1445. Failure to introduce mutations using Overlap PCR - (reply: 3)
1446. no or poor amplification - (reply: 1)
1447. Primers from 3'UTR - (reply: 2)
1448. Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
1449. Designing primers with ESTs - (reply: 2)
1450. Ct variation/Ct limit/rē values/efficiency errors - (reply: 2)
1451. Probe manufacturers, who makes best probes? - Where do you get your probes and are you happy with them? (reply: 3)
1452. Real Time vs Traditional PCR results - (reply: 1)
1453. The problems to identify mouse genotype with PCR - (reply: 1)
1454. non specific amplification - DNA amplification by PCR (reply: 1)
1455. RNA Extraction - Extraction of Yeast RNA from mice blood (reply: 1)
1456. Large Difference between Ct of 18s rRNA and Target Gene - (reply: 2)
1457. PCR has stopped working - (reply: 7)
1458. PCR negative control contamination - (reply: 8)
1459. Real-Time PCR - Gene expression (reply: 2)
1460. PCR efficiency in real timePCR - (reply: 5)
1461. Plate setup for qRT-PCR - Too many samples for one plate... (reply: 1)
1462. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
1463. QPCR analysis with various # of samples from 1 patient? - (reply: 1)
1464. Drastic Decrease in PCR product yield - (reply: 2)
1465. Contamination? - (reply: 1)
1466. multiplexing taqman miRNA reverse transcription - (reply: 2)
1467. ligation problem - (reply: 3)
1468. Analyzing qPCR results - (reply: 1)
1469. resources on PCR principles & technique - (reply: 1)
1470. inconsistent results - easy 16S amplifcation is not so easy - (reply: 1)