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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1441. DNA and RNA contamination in RT PCR water controls - (reply: 2)
1442. Optimal target length - (reply: 3)
1443. Fermentas SYBR Green vs. Qiagen SYBR Green - (reply: 2)
1444. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
1445. Different types/Brands of BSA? - (reply: 2)
1446. NTC with specific amplification - (reply: 1)
1447. Lost a Band by changing thermocycler - (reply: 9)
1448. Primer3 vs Primer BLAST - (reply: 3)
1449. qPCR using standard curve method - how to do correct setup? (reply: 3)
1450. Primer-Blast - (reply: 3)
1451. Amplification in NTC and noRT controls - (reply: 5)
1452. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
1453. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
1454. asymmetric PCR - (reply: 1)
1455. Best time to isolate RNA for qPCR? - (reply: 1)
1456. PCR amplifying 50bp ssDNA ? - PCR amplification (reply: 6)
1457. CORRECT PCR Incorrect RTPCR - (reply: 10)
1458. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
1459. Confusing bands from PCR - not primer dimer, not product (reply: 30)
1460. Real time PCR results-interpretation - (reply: 2)
1461. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
1462. Bands appearing out of no where?! - (reply: 5)
1463. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
1464. SYBR melting curve vs RT-PCR gel - (reply: 1)
1465. searching housekeeping genes - (reply: 4)
1466. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
1467. primer tm - tm calculation for tagged primers (reply: 2)
1468. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
1469. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
1470. Long real-time Amplicon - (reply: 2)