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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1441. Primer3 vs Primer BLAST - (reply: 3)
1442. qPCR using standard curve method - how to do correct setup? (reply: 3)
1443. Primer-Blast - (reply: 3)
1444. Amplification in NTC and noRT controls - (reply: 5)
1445. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
1446. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
1447. asymmetric PCR - (reply: 1)
1448. Best time to isolate RNA for qPCR? - (reply: 1)
1449. PCR amplifying 50bp ssDNA ? - PCR amplification (reply: 6)
1450. CORRECT PCR Incorrect RTPCR - (reply: 10)
1451. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
1452. Confusing bands from PCR - not primer dimer, not product (reply: 30)
1453. Real time PCR results-interpretation - (reply: 2)
1454. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
1455. Bands appearing out of no where?! - (reply: 5)
1456. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
1457. SYBR melting curve vs RT-PCR gel - (reply: 1)
1458. searching housekeeping genes - (reply: 4)
1459. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
1460. primer tm - tm calculation for tagged primers (reply: 2)
1461. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
1462. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
1463. Long real-time Amplicon - (reply: 2)
1464. PCR + phusion enzyme = massive errors - (reply: 4)
1465. help needed in cDNA synthesis - needed help desperately,tq (reply: 3)
1466. RNA from MMA embedded calcified bone - (reply: 1)
1467. long DNA amplification - (reply: 11)
1468. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
1469. gDNA pcr product as standard for absolute quantification? - (reply: 1)
1470. qPCR - no signal detected but plenty of product on gel - (reply: 3)