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1441. Primers and annealing temperature - (reply: 2)
1442. Bacteria gene expression - (reply: 2)
1443. Copy number calculations in real time PCR - (reply: 2)
1444. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
1445. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
1446. RT-PCR Negative controls - (reply: 1)
1447. Problems with qRT-PCR - (reply: 3)
1448. PCR with 60 bp primers - results in no product (reply: 6)
1449. Shrimp nuclease to eliminate genomic DNA - (reply: 7)
1450. How do you manually determine Ct value? - (reply: 1)
1451. Running gel after RT-PCR - (reply: 5)
1452. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
1453. Etbr staining methods - (reply: 6)
1454. PCR problems.. - problems with conventional PCR (reply: 3)
1455. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
1456. gDNA or cDNA amplification in RT-PCR - (reply: 3)
1457. Dnase digestion and PCR - (reply: 4)
1458. Understanding qPCR and gene expression changes - (reply: 10)
1459. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
1460. Reverse Transcription - (reply: 3)
1461. Plasmid as standard - Plasmid calculation (reply: 6)
1462. what Ct values are considered ideal? too high? - (reply: 1)
1463. Need an UV260 RT-PCR instrument - (reply: 1)
1464. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
1465. PCR produces products of wrong size - (reply: 4)
1466. PCR with pfu and degenerate primers (?) - (reply: 7)
1467. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
1468. Serious issues with RT-PCR - (reply: 7)
1469. relative quantitation problem plz help - (reply: 4)
1470. No beta actin results at all??? - (reply: 1)
1442. Bacteria gene expression - (reply: 2)
1443. Copy number calculations in real time PCR - (reply: 2)
1444. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
1445. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
1446. RT-PCR Negative controls - (reply: 1)
1447. Problems with qRT-PCR - (reply: 3)
1448. PCR with 60 bp primers - results in no product (reply: 6)
1449. Shrimp nuclease to eliminate genomic DNA - (reply: 7)
1450. How do you manually determine Ct value? - (reply: 1)
1451. Running gel after RT-PCR - (reply: 5)
1452. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
1453. Etbr staining methods - (reply: 6)
1454. PCR problems.. - problems with conventional PCR (reply: 3)
1455. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
1456. gDNA or cDNA amplification in RT-PCR - (reply: 3)
1457. Dnase digestion and PCR - (reply: 4)
1458. Understanding qPCR and gene expression changes - (reply: 10)
1459. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
1460. Reverse Transcription - (reply: 3)
1461. Plasmid as standard - Plasmid calculation (reply: 6)
1462. what Ct values are considered ideal? too high? - (reply: 1)
1463. Need an UV260 RT-PCR instrument - (reply: 1)
1464. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
1465. PCR produces products of wrong size - (reply: 4)
1466. PCR with pfu and degenerate primers (?) - (reply: 7)
1467. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
1468. Serious issues with RT-PCR - (reply: 7)
1469. relative quantitation problem plz help - (reply: 4)
1470. No beta actin results at all??? - (reply: 1)