Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
901. nested PCR with low target DNA? - (reply: 2)
902. RT-PCR calibrator - (reply: 2)
903. problem in pcr - (reply: 2)
904. Can someone recommend me a virtual PCR software? - (reply: 6)
905. negative values after GOI - reference gene ?? - (reply: 4)
906. RNA degradation during electrophoresis?? - (reply: 2)
907. Primer 3' mismatch - Strange experience (reply: 9)
908. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
909. Adding loading/tracking dye to PCR mix? - (reply: 10)
910. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
911. internal control in RT-PCR - inquiry (reply: 2)
912. Melting Temp of Standards Changed? - (reply: 3)
913. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
914. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
915. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
916. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
917. Gene on negative strand - two eductional questions. - (reply: 3)
918. Newbie- choose DNA template - (reply: 1)
919. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
920. Total RNA estimation - (reply: 2)
921. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
922. pcr contamination - (reply: 4)
923. Large Non-specfic bands in PCR - (reply: 3)
924. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
925. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
926. Primer dilution --> problem.. - (reply: 16)
927. no bands after pcr - even after we have change most of the reagents (reply: 2)
928. High resolution melting to gene scanning - (reply: 1)
929. Help! Smear in Real-Time PCR Product - (reply: 1)
930. Is one reference gene ever appropriate? - (reply: 3)