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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
901. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
902. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
903. can contaminating gDNA explain this? - (reply: 1)
904. TOUCH DOWN PCR - (reply: 2)
905. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
906. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
907. PCR stopped working - After changing buffer (reply: 5)
908. how to determine the amount of cDNA for qPCR - (reply: 5)
909. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)
910. Standardization of HRM for methylation analysis - (reply: 4)
911. PCR protocol questions! - (reply: 2)
912. standard curve for qPCR - (reply: 3)
913. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
914. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
915. DNA amount calculation for PCR - (reply: 14)
916. ChIP primer design - (reply: 1)
917. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
918. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
919. weird QPCR curves - (reply: 2)
920. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
921. Questions regarding RT-PCR optimization - (reply: 2)
922. Problems with semi-quatitative PCR - (reply: 1)
923. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
924. Long-amplicon QPCR of mtDNA - (reply: 5)
925. PCR reaction calculation - (reply: 2)
926. Qiagen rotorgene syber green kit reaction volume - (reply: 1)
927. missing nt at the 5' end - site mutagensis using NEB kit (reply: 1)
928. no increase in fluorescence in my real time PCR - (reply: 3)
929. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
930. Roche second derivative maximum - can be treated according to Pfaffl? (reply: 1)