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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
901. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
902. Total RNA estimation - (reply: 2)
903. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
904. pcr contamination - (reply: 4)
905. Large Non-specfic bands in PCR - (reply: 3)
906. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
907. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
908. Primer dilution --> problem.. - (reply: 16)
909. no bands after pcr - even after we have change most of the reagents (reply: 2)
910. High resolution melting to gene scanning - (reply: 1)
911. Help! Smear in Real-Time PCR Product - (reply: 1)
912. Is one reference gene ever appropriate? - (reply: 3)
913. Sybr green RT-qPCR primer - (reply: 1)
914. Sybr green RT-qPCR primer - (reply: 1)
915. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
916. Oligo Question - (reply: 1)
917. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
918. real time rt PCR - bloodyurine - inhibition - (reply: 1)
919. No Amplification in PCR - (reply: 4)
920. DNase treatment after RNA extraction using RNeasy kit of qiagen - DNaes treatment after RNA extraction (reply: 2)
921. Real time PCR and percentage loss - (reply: 1)
922. Problem with smear in PCR with certain templates - (reply: 4)
923. running multiple qPCR with common housekeeping control - (reply: 3)
924. Problems with PCR in general - (reply: 1)
925. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 2)
926. Control inconsistence in Q-PCR - (reply: 1)
927. how to overcome primer contamination - need protocol (reply: 11)
928. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
929. sequencing problem - (reply: 1)
930. High Resolution Melting (HRM) Problem - (reply: 4)