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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
901. Large Non-specfic bands in PCR - (reply: 3)
902. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
903. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
904. Primer dilution --> problem.. - (reply: 16)
905. no bands after pcr - even after we have change most of the reagents (reply: 2)
906. High resolution melting to gene scanning - (reply: 1)
907. Help! Smear in Real-Time PCR Product - (reply: 1)
908. Is one reference gene ever appropriate? - (reply: 3)
909. Sybr green RT-qPCR primer - (reply: 1)
910. Sybr green RT-qPCR primer - (reply: 1)
911. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
912. Oligo Question - (reply: 1)
913. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
914. real time rt PCR - bloodyurine - inhibition - (reply: 1)
915. No Amplification in PCR - (reply: 4)
916. DNase treatment after RNA extraction using RNeasy kit of qiagen - DNaes treatment after RNA extraction (reply: 2)
917. Real time PCR and percentage loss - (reply: 1)
918. Problem with smear in PCR with certain templates - (reply: 4)
919. running multiple qPCR with common housekeeping control - (reply: 3)
920. Problems with PCR in general - (reply: 1)
921. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 2)
922. Control inconsistence in Q-PCR - (reply: 1)
923. how to overcome primer contamination - need protocol (reply: 11)
924. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
925. sequencing problem - (reply: 1)
926. High Resolution Melting (HRM) Problem - (reply: 4)
927. Consistancy in qPCR data - (reply: 5)
928. RT-PCR primer design - Primer design and evaluation (reply: 7)
929. qPCR with low expressing genes - (reply: 1)
930. Product size? - (reply: 7)