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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
901. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
902. Oligo Question - (reply: 1)
903. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
904. real time rt PCR - bloodyurine - inhibition - (reply: 1)
905. No Amplification in PCR - (reply: 4)
906. DNase treatment after RNA extraction using RNeasy kit of qiagen - DNaes treatment after RNA extraction (reply: 2)
907. Real time PCR and percentage loss - (reply: 1)
908. Problem with smear in PCR with certain templates - (reply: 4)
909. running multiple qPCR with common housekeeping control - (reply: 3)
910. Problems with PCR in general - (reply: 1)
911. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 2)
912. Control inconsistence in Q-PCR - (reply: 1)
913. how to overcome primer contamination - need protocol (reply: 11)
914. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
915. sequencing problem - (reply: 1)
916. High Resolution Melting (HRM) Problem - (reply: 4)
917. Consistancy in qPCR data - (reply: 5)
918. RT-PCR primer design - Primer design and evaluation (reply: 7)
919. qPCR with low expressing genes - (reply: 1)
920. Product size? - (reply: 7)
921. Contamination issues - (reply: 5)
922. PCRing small region on genomic DNA - (reply: 3)
923. Confirm RACE product - (reply: 3)
924. Reference genes. - (reply: 1)
925. Two peaks generated by one NTC on melting curve. - (reply: 2)
926. Splice varient quantification in RT real time PCR? - (reply: 1)
927. Help with delta delta ct analysis - (reply: 1)
928. Calculating geometric mean for real-time PCR - (reply: 5)
929. semiquantitative pcr - (reply: 1)
930. difference in band sizes ! - why ??? (reply: 2)