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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
901. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
902. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
903. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
904. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
905. Gene on negative strand - two eductional questions. - (reply: 3)
906. Newbie- choose DNA template - (reply: 1)
907. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
908. Total RNA estimation - (reply: 2)
909. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
910. pcr contamination - (reply: 4)
911. Large Non-specfic bands in PCR - (reply: 3)
912. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
913. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
914. Primer dilution --> problem.. - (reply: 16)
915. no bands after pcr - even after we have change most of the reagents (reply: 2)
916. High resolution melting to gene scanning - (reply: 1)
917. Help! Smear in Real-Time PCR Product - (reply: 1)
918. Is one reference gene ever appropriate? - (reply: 3)
919. Sybr green RT-qPCR primer - (reply: 1)
920. Sybr green RT-qPCR primer - (reply: 1)
921. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
922. Oligo Question - (reply: 1)
923. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
924. real time rt PCR - bloodyurine - inhibition - (reply: 1)
925. No Amplification in PCR - (reply: 4)
926. DNase treatment after RNA extraction using RNeasy kit of qiagen - DNaes treatment after RNA extraction (reply: 2)
927. Real time PCR and percentage loss - (reply: 1)
928. Problem with smear in PCR with certain templates - (reply: 4)
929. running multiple qPCR with common housekeeping control - (reply: 3)
930. Problems with PCR in general - (reply: 1)