Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
901. Standardization of HRM for methylation analysis - (reply: 4)
902. PCR protocol questions! - (reply: 2)
903. standard curve for qPCR - (reply: 3)
904. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
905. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
906. DNA amount calculation for PCR - (reply: 14)
907. ChIP primer design - (reply: 1)
908. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
909. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
910. weird QPCR curves - (reply: 2)
911. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
912. Questions regarding RT-PCR optimization - (reply: 2)
913. Problems with semi-quatitative PCR - (reply: 1)
914. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
915. Long-amplicon QPCR of mtDNA - (reply: 5)
916. PCR reaction calculation - (reply: 2)
917. Qiagen rotorgene syber green kit reaction volume - (reply: 1)
918. missing nt at the 5' end - site mutagensis using NEB kit (reply: 1)
919. no increase in fluorescence in my real time PCR - (reply: 3)
920. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
921. Roche second derivative maximum - can be treated according to Pfaffl? (reply: 1)
922. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
923. Pooled RNA from healthy controls - delta-delta Ct method (reply: 1)
924. Probe Dilution Problem = 100uM to 5ng/ul? + Final Volume for FISH - Probe dilution and FISH - Advise on volume for FISH hibridization buff (reply: 2)
925. PCR, using gel extracted band as a template - (reply: 4)
926. Question of nucleic acid extraction - (reply: 1)
927. Multiplex problems - (reply: 6)
928. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
929. Q-PCR and RT-PCR - (reply: 3)
930. inter-run calibration - (reply: 4)