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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
901. negative values after GOI - reference gene ?? - (reply: 4)
902. RNA degradation during electrophoresis?? - (reply: 2)
903. Primer 3' mismatch - Strange experience (reply: 9)
904. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
905. Adding loading/tracking dye to PCR mix? - (reply: 10)
906. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
907. internal control in RT-PCR - inquiry (reply: 2)
908. Melting Temp of Standards Changed? - (reply: 3)
909. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
910. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
911. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
912. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
913. Gene on negative strand - two eductional questions. - (reply: 3)
914. Newbie- choose DNA template - (reply: 1)
915. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
916. Total RNA estimation - (reply: 2)
917. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
918. pcr contamination - (reply: 4)
919. Large Non-specfic bands in PCR - (reply: 3)
920. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
921. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
922. Primer dilution --> problem.. - (reply: 16)
923. no bands after pcr - even after we have change most of the reagents (reply: 2)
924. High resolution melting to gene scanning - (reply: 1)
925. Help! Smear in Real-Time PCR Product - (reply: 1)
926. Is one reference gene ever appropriate? - (reply: 3)
927. Sybr green RT-qPCR primer - (reply: 1)
928. Sybr green RT-qPCR primer - (reply: 1)
929. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
930. Oligo Question - (reply: 1)