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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
901. Detection limit of a conventional PCR - (reply: 2)
902. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
903. RNAi showing upregulation via QPCR - Help needed (reply: 3)
904. DNA detection limit of the PCR - Calculating detection limits (reply: 4)
905. cDNA synthesis - (reply: 3)
906. 2 rounds PCR got problem - (reply: 2)
907. PCR help minus strand - Primer design (reply: 1)
908. cDNA contamination, pls help! - (reply: 1)
909. Smear in long distance PCR - (reply: 39)
910. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
911. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
912. can contaminating gDNA explain this? - (reply: 1)
913. TOUCH DOWN PCR - (reply: 2)
914. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
915. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
916. PCR stopped working - After changing buffer (reply: 5)
917. how to determine the amount of cDNA for qPCR - (reply: 5)
918. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)
919. Standardization of HRM for methylation analysis - (reply: 4)
920. PCR protocol questions! - (reply: 2)
921. standard curve for qPCR - (reply: 3)
922. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
923. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
924. DNA amount calculation for PCR - (reply: 14)
925. ChIP primer design - (reply: 1)
926. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
927. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
928. weird QPCR curves - (reply: 2)
929. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
930. Questions regarding RT-PCR optimization - (reply: 2)