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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
901. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
902. LacZ PCR Problems - (reply: 2)
903. Primer concentration - (reply: 4)
904. nested PCR with low target DNA? - (reply: 2)
905. RT-PCR calibrator - (reply: 2)
906. problem in pcr - (reply: 2)
907. Can someone recommend me a virtual PCR software? - (reply: 6)
908. negative values after GOI - reference gene ?? - (reply: 4)
909. RNA degradation during electrophoresis?? - (reply: 2)
910. Primer 3' mismatch - Strange experience (reply: 9)
911. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
912. Adding loading/tracking dye to PCR mix? - (reply: 10)
913. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
914. internal control in RT-PCR - inquiry (reply: 2)
915. Melting Temp of Standards Changed? - (reply: 3)
916. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
917. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
918. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
919. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
920. Gene on negative strand - two eductional questions. - (reply: 3)
921. Newbie- choose DNA template - (reply: 1)
922. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
923. Total RNA estimation - (reply: 2)
924. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
925. pcr contamination - (reply: 4)
926. Large Non-specfic bands in PCR - (reply: 3)
927. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
928. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
929. Primer dilution --> problem.. - (reply: 16)
930. no bands after pcr - even after we have change most of the reagents (reply: 2)