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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
901. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
902. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
903. Primer dilution --> problem.. - (reply: 16)
904. no bands after pcr - even after we have change most of the reagents (reply: 2)
905. High resolution melting to gene scanning - (reply: 1)
906. Help! Smear in Real-Time PCR Product - (reply: 1)
907. Is one reference gene ever appropriate? - (reply: 3)
908. Sybr green RT-qPCR primer - (reply: 1)
909. Sybr green RT-qPCR primer - (reply: 1)
910. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
911. Oligo Question - (reply: 1)
912. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
913. real time rt PCR - bloodyurine - inhibition - (reply: 1)
914. No Amplification in PCR - (reply: 4)
915. DNase treatment after RNA extraction using RNeasy kit of qiagen - DNaes treatment after RNA extraction (reply: 2)
916. Real time PCR and percentage loss - (reply: 1)
917. Problem with smear in PCR with certain templates - (reply: 4)
918. running multiple qPCR with common housekeeping control - (reply: 3)
919. Problems with PCR in general - (reply: 1)
920. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 2)
921. Control inconsistence in Q-PCR - (reply: 1)
922. how to overcome primer contamination - need protocol (reply: 11)
923. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
924. sequencing problem - (reply: 1)
925. High Resolution Melting (HRM) Problem - (reply: 4)
926. Consistancy in qPCR data - (reply: 5)
927. RT-PCR primer design - Primer design and evaluation (reply: 7)
928. qPCR with low expressing genes - (reply: 1)
929. Product size? - (reply: 7)
930. Contamination issues - (reply: 5)