Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
901. Primer 3' mismatch - Strange experience (reply: 9)
902. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
903. Adding loading/tracking dye to PCR mix? - (reply: 10)
904. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
905. internal control in RT-PCR - inquiry (reply: 2)
906. Melting Temp of Standards Changed? - (reply: 3)
907. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
908. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
909. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
910. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
911. Gene on negative strand - two eductional questions. - (reply: 3)
912. Newbie- choose DNA template - (reply: 1)
913. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
914. Total RNA estimation - (reply: 2)
915. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
916. pcr contamination - (reply: 4)
917. Large Non-specfic bands in PCR - (reply: 3)
918. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
919. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
920. Primer dilution --> problem.. - (reply: 16)
921. no bands after pcr - even after we have change most of the reagents (reply: 2)
922. High resolution melting to gene scanning - (reply: 1)
923. Help! Smear in Real-Time PCR Product - (reply: 1)
924. Is one reference gene ever appropriate? - (reply: 3)
925. Sybr green RT-qPCR primer - (reply: 1)
926. Sybr green RT-qPCR primer - (reply: 1)
927. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
928. Oligo Question - (reply: 1)
929. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
930. real time rt PCR - bloodyurine - inhibition - (reply: 1)