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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1561. miScript (Qiagen) - RT of both miRNA and mRNA - does it work? (reply: 1)
1562. high Cp value PCR - (reply: 2)
1563. real time PCR data presentation - presentation of delta CT and copy number (reply: 2)
1564. Synthesize own Oligo-dT primers - (reply: 6)
1565. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
1566. Cell-cycle independant qRT-PCR internal control - anyone has experience doing this? (reply: 1)
1567. dNTP or Deoxynucleotide solution Mix - (reply: 1)
1568. Smearing/ no amplification in PCR - (reply: 3)
1569. qRTPCR (TaqMan) Data Representation - (reply: 1)
1570. mRNA versus protein expression. Why inverse correlation? - (reply: 3)
1571. Lucicferase activity and endogenous transcription - (reply: 7)
1572. need help on primer calcuation soon - (reply: 1)
1573. Heart ache in real time - (reply: 1)
1574. HEX vs. Cy-5 - (reply: 3)
1575. Direct PCR (PCR without DNA extraction) - (reply: 2)
1576. PCR using xt5 - (reply: 2)
1577. long range PCR - purification of pcr frogament 9-13kb (reply: 9)
1578. Problems with qRT-PCR - Lots of unspecific products (reply: 2)
1579. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
1580. 2 bands or smear? 2-step RT-PCR - (reply: 1)
1581. 23S rRNA Primer - need primer sequence (reply: 1)
1582. Lowered extension temps with Phusion - (reply: 7)
1583. RT-PCR - problems with TaqMan PCR (reply: 2)
1584. qRT-PCR calculations - Averaging across biological replicates (reply: 2)
1585. Two annealing temps? - (reply: 4)
1586. Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
1587. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
1588. Small product amplification problems - (reply: 4)
1589. ROX and Rotorgene - using a mix with reference dye in Rotorgene (reply: 2)
1590. Freezing primers+SYBR green - (reply: 2)