Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1561. Primer3 vs Primer BLAST - (reply: 3)
1562. qPCR using standard curve method - how to do correct setup? (reply: 3)
1563. Primer-Blast - (reply: 3)
1564. Amplification in NTC and noRT controls - (reply: 5)
1565. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
1566. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
1567. asymmetric PCR - (reply: 1)
1568. Best time to isolate RNA for qPCR? - (reply: 1)
1569. PCR amplifying 50bp ssDNA ? - PCR amplification (reply: 6)
1570. CORRECT PCR Incorrect RTPCR - (reply: 10)
1571. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
1572. Confusing bands from PCR - not primer dimer, not product (reply: 30)
1573. Real time PCR results-interpretation - (reply: 2)
1574. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
1575. Bands appearing out of no where?! - (reply: 5)
1576. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
1577. SYBR melting curve vs RT-PCR gel - (reply: 1)
1578. searching housekeeping genes - (reply: 4)
1579. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
1580. primer tm - tm calculation for tagged primers (reply: 2)
1581. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
1582. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
1583. Long real-time Amplicon - (reply: 2)
1584. PCR + phusion enzyme = massive errors - (reply: 4)
1585. help needed in cDNA synthesis - needed help desperately,tq (reply: 3)
1586. RNA from MMA embedded calcified bone - (reply: 1)
1587. long DNA amplification - (reply: 11)
1588. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
1589. gDNA pcr product as standard for absolute quantification? - (reply: 1)
1590. qPCR - no signal detected but plenty of product on gel - (reply: 3)