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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1561. Different types/Brands of BSA? - (reply: 2)
1562. NTC with specific amplification - (reply: 1)
1563. Lost a Band by changing thermocycler - (reply: 9)
1564. Primer3 vs Primer BLAST - (reply: 3)
1565. qPCR using standard curve method - how to do correct setup? (reply: 3)
1566. Primer-Blast - (reply: 3)
1567. Amplification in NTC and noRT controls - (reply: 5)
1568. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
1569. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
1570. asymmetric PCR - (reply: 1)
1571. Best time to isolate RNA for qPCR? - (reply: 1)
1572. PCR amplifying 50bp ssDNA ? - PCR amplification (reply: 6)
1573. CORRECT PCR Incorrect RTPCR - (reply: 10)
1574. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
1575. Confusing bands from PCR - not primer dimer, not product (reply: 30)
1576. Real time PCR results-interpretation - (reply: 2)
1577. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
1578. Bands appearing out of no where?! - (reply: 5)
1579. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
1580. SYBR melting curve vs RT-PCR gel - (reply: 1)
1581. searching housekeeping genes - (reply: 4)
1582. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
1583. primer tm - tm calculation for tagged primers (reply: 2)
1584. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
1585. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
1586. Long real-time Amplicon - (reply: 2)
1587. PCR + phusion enzyme = massive errors - (reply: 4)
1588. help needed in cDNA synthesis - needed help desperately,tq (reply: 3)
1589. RNA from MMA embedded calcified bone - (reply: 1)
1590. long DNA amplification - (reply: 11)