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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1561. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
1562. QPCR problem! - (reply: 9)
1563. pcr trouble solved but I cant seem to understand why - (reply: 1)
1564. Primer design if sequence is unknown for organism - (reply: 5)
1565. Reference gene qPCR - (reply: 4)
1566. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
1567. Real time PCR info - (reply: 4)
1568. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
1569. standard curve - (reply: 2)
1570. PCR from BAC - PCR from BAC (reply: 1)
1571. Mean versus Mediat Ct Values - Which to use for statistical analsyis (reply: 1)
1572. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
1573. High Molecular weight artifact LA-PCR 2-step - Artifact or real band - higher than expected Mw amplified product (reply: 3)
1574. heating rna before rt reaction? - (reply: 1)
1575. relative gene expression with multiple reference genes - data analysis (reply: 1)
1576. qRT-PCR no of cycles?? - (reply: 6)
1577. Is there any setting to avoid same base runs in Primer-BLAST? - (reply: 3)
1578. Which techniques should i do? - Which techniques should i do? (reply: 2)
1579. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
1580. what primers should I use for DNA sequencing? please help - (reply: 2)
1581. Standard curve for RT-PCR - (reply: 1)
1582. Problem amplifying plasmid - (reply: 1)
1583. What Factors Affect Ct values of a gene? - (reply: 1)
1584. Sybr Green I in real-time PCR reaction - (reply: 3)
1585. Primers annealing temperatures - (reply: 8)
1586. Difficulties cloning by PCR - (reply: 5)
1587. PCR Result Explanation (I needed you help) - Refer to the result, what is indicate A? (reply: 4)
1588. rna storage at -20 - (reply: 2)
1589. miScript (Qiagen) - RT of both miRNA and mRNA - does it work? (reply: 1)
1590. high Cp value PCR - (reply: 2)