Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1561. Multiplex PCR - (reply: 1)
1562. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
1563. optimal template dna concentrations - (reply: 1)
1564. reconstitution of primers - (reply: 2)
1565. Mysterious PCR Contamination - (reply: 13)
1566. RT-PCR Help - Protocol provided (reply: 4)
1567. Endogenous control in bacterial RT-qPCR - (reply: 4)
1568. Mutagenesis PCR and undesired amplification - (reply: 2)
1569. PCR-how to set 'Extension: ramping from 55 to 72 for 5min' - (reply: 4)
1570. primer dimer - (reply: 2)
1571. Genotyping conditioning-where to start? - (reply: 1)
1572. Marker migrates slower depending where is loaded - (reply: 2)
1573. PCR on degraded templates - (reply: 2)
1574. PCR help needed - (reply: 3)
1575. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
1576. Tm difference - (reply: 2)
1577. Genotyping - PCR not working (reply: 6)
1578. RT qPCR Newbie, Weird Results - (reply: 1)
1579. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
1580. light bands, dark smear, dark dimers - (reply: 2)
1581. PCR, followed by sequencing... - why ?? (reply: 3)
1582. qPCR and varying cDNA concentrations - (reply: 1)
1583. PCR program - strange extension step (reply: 2)
1584. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
1585. Trouble with GAPDH housekeeping gene - (reply: 3)
1586. REAL TIME PCR 7005 v 2.01 : I need more reference samples! - How to add more than one reference sample to Real Time?? (reply: 1)
1587. qPCR lacks reproduceability - perfect runs, no reproduceability, no explaination, desperate... (reply: 5)
1588. Using BLAST to check primers - (reply: 17)
1589. qPCR and quantity of RNA - (reply: 2)
1590. pcr doesnt show any band for DNa walking - (reply: 8)