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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1561. DNA and RNA contamination in RT PCR water controls - (reply: 2)
1562. Optimal target length - (reply: 3)
1563. Fermentas SYBR Green vs. Qiagen SYBR Green - (reply: 2)
1564. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
1565. Different types/Brands of BSA? - (reply: 2)
1566. NTC with specific amplification - (reply: 1)
1567. Lost a Band by changing thermocycler - (reply: 9)
1568. Primer3 vs Primer BLAST - (reply: 3)
1569. qPCR using standard curve method - how to do correct setup? (reply: 3)
1570. Primer-Blast - (reply: 3)
1571. Amplification in NTC and noRT controls - (reply: 5)
1572. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
1573. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
1574. asymmetric PCR - (reply: 1)
1575. Best time to isolate RNA for qPCR? - (reply: 1)
1576. PCR amplifying 50bp ssDNA ? - PCR amplification (reply: 6)
1577. CORRECT PCR Incorrect RTPCR - (reply: 10)
1578. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
1579. Confusing bands from PCR - not primer dimer, not product (reply: 30)
1580. Real time PCR results-interpretation - (reply: 2)
1581. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
1582. Bands appearing out of no where?! - (reply: 5)
1583. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
1584. SYBR melting curve vs RT-PCR gel - (reply: 1)
1585. searching housekeeping genes - (reply: 4)
1586. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
1587. primer tm - tm calculation for tagged primers (reply: 2)
1588. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
1589. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
1590. Long real-time Amplicon - (reply: 2)