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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1651. HEX vs. Cy-5 - (reply: 3)
1652. Direct PCR (PCR without DNA extraction) - (reply: 2)
1653. PCR using xt5 - (reply: 2)
1654. long range PCR - purification of pcr frogament 9-13kb (reply: 9)
1655. Problems with qRT-PCR - Lots of unspecific products (reply: 2)
1656. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
1657. 2 bands or smear? 2-step RT-PCR - (reply: 1)
1658. 23S rRNA Primer - need primer sequence (reply: 1)
1659. Lowered extension temps with Phusion - (reply: 7)
1660. RT-PCR - problems with TaqMan PCR (reply: 2)
1661. qRT-PCR calculations - Averaging across biological replicates (reply: 2)
1662. Two annealing temps? - (reply: 4)
1663. Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
1664. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
1665. Small product amplification problems - (reply: 4)
1666. ROX and Rotorgene - using a mix with reference dye in Rotorgene (reply: 2)
1667. Freezing primers+SYBR green - (reply: 2)
1668. Tagged Primers - (reply: 5)
1669. no pcr amplification product - (reply: 9)
1670. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
1671. less steep curve and high Ct value! - (reply: 2)
1672. Question about site directed mutagenesis - (reply: 1)
1673. Amplfication of fungal DNA - (reply: 8)
1674. PCR problem - (reply: 4)
1675. mRNA level in transgene cell line or knocking down cell line - (reply: 3)
1676. differently behavioring replicates in real-time PCR - (reply: 1)
1677. Multiplex PCR - (reply: 1)
1678. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
1679. optimal template dna concentrations - (reply: 1)
1680. reconstitution of primers - (reply: 2)