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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1651. ROX and Rotorgene - using a mix with reference dye in Rotorgene (reply: 2)
1652. Freezing primers+SYBR green - (reply: 2)
1653. Tagged Primers - (reply: 5)
1654. no pcr amplification product - (reply: 9)
1655. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
1656. less steep curve and high Ct value! - (reply: 2)
1657. Question about site directed mutagenesis - (reply: 1)
1658. Amplfication of fungal DNA - (reply: 8)
1659. PCR problem - (reply: 4)
1660. mRNA level in transgene cell line or knocking down cell line - (reply: 3)
1661. differently behavioring replicates in real-time PCR - (reply: 1)
1662. Multiplex PCR - (reply: 1)
1663. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
1664. optimal template dna concentrations - (reply: 1)
1665. reconstitution of primers - (reply: 2)
1666. Mysterious PCR Contamination - (reply: 13)
1667. RT-PCR Help - Protocol provided (reply: 4)
1668. Endogenous control in bacterial RT-qPCR - (reply: 4)
1669. Mutagenesis PCR and undesired amplification - (reply: 2)
1670. PCR-how to set 'Extension: ramping from 55 to 72 for 5min' - (reply: 4)
1671. primer dimer - (reply: 2)
1672. Genotyping conditioning-where to start? - (reply: 1)
1673. Marker migrates slower depending where is loaded - (reply: 2)
1674. PCR on degraded templates - (reply: 2)
1675. PCR help needed - (reply: 3)
1676. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
1677. Tm difference - (reply: 2)
1678. Genotyping - PCR not working (reply: 6)
1679. RT qPCR Newbie, Weird Results - (reply: 1)
1680. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)