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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1651. what primers should I use for DNA sequencing? please help - (reply: 2)
1652. Standard curve for RT-PCR - (reply: 1)
1653. Problem amplifying plasmid - (reply: 1)
1654. What Factors Affect Ct values of a gene? - (reply: 1)
1655. Sybr Green I in real-time PCR reaction - (reply: 3)
1656. Primers annealing temperatures - (reply: 8)
1657. Difficulties cloning by PCR - (reply: 5)
1658. PCR Result Explanation (I needed you help) - Refer to the result, what is indicate A? (reply: 4)
1659. rna storage at -20 - (reply: 2)
1660. miScript (Qiagen) - RT of both miRNA and mRNA - does it work? (reply: 1)
1661. high Cp value PCR - (reply: 2)
1662. real time PCR data presentation - presentation of delta CT and copy number (reply: 2)
1663. Synthesize own Oligo-dT primers - (reply: 6)
1664. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
1665. Cell-cycle independant qRT-PCR internal control - anyone has experience doing this? (reply: 1)
1666. dNTP or Deoxynucleotide solution Mix - (reply: 1)
1667. Smearing/ no amplification in PCR - (reply: 3)
1668. qRTPCR (TaqMan) Data Representation - (reply: 1)
1669. mRNA versus protein expression. Why inverse correlation? - (reply: 3)
1670. Lucicferase activity and endogenous transcription - (reply: 7)
1671. need help on primer calcuation soon - (reply: 1)
1672. Heart ache in real time - (reply: 1)
1673. HEX vs. Cy-5 - (reply: 3)
1674. Direct PCR (PCR without DNA extraction) - (reply: 2)
1675. PCR using xt5 - (reply: 2)
1676. long range PCR - purification of pcr frogament 9-13kb (reply: 9)
1677. Problems with qRT-PCR - Lots of unspecific products (reply: 2)
1678. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
1679. 2 bands or smear? 2-step RT-PCR - (reply: 1)
1680. 23S rRNA Primer - need primer sequence (reply: 1)