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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1651. mRNA level in transgene cell line or knocking down cell line - (reply: 3)
1652. differently behavioring replicates in real-time PCR - (reply: 1)
1653. Multiplex PCR - (reply: 1)
1654. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
1655. optimal template dna concentrations - (reply: 1)
1656. reconstitution of primers - (reply: 2)
1657. Mysterious PCR Contamination - (reply: 13)
1658. RT-PCR Help - Protocol provided (reply: 4)
1659. Endogenous control in bacterial RT-qPCR - (reply: 4)
1660. Mutagenesis PCR and undesired amplification - (reply: 2)
1661. PCR-how to set 'Extension: ramping from 55 to 72 for 5min' - (reply: 4)
1662. primer dimer - (reply: 2)
1663. Genotyping conditioning-where to start? - (reply: 1)
1664. Marker migrates slower depending where is loaded - (reply: 2)
1665. PCR on degraded templates - (reply: 2)
1666. PCR help needed - (reply: 3)
1667. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
1668. Tm difference - (reply: 2)
1669. Genotyping - PCR not working (reply: 6)
1670. RT qPCR Newbie, Weird Results - (reply: 1)
1671. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
1672. light bands, dark smear, dark dimers - (reply: 2)
1673. PCR, followed by sequencing... - why ?? (reply: 3)
1674. qPCR and varying cDNA concentrations - (reply: 1)
1675. PCR program - strange extension step (reply: 2)
1676. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
1677. Trouble with GAPDH housekeeping gene - (reply: 3)
1678. REAL TIME PCR 7005 v 2.01 : I need more reference samples! - How to add more than one reference sample to Real Time?? (reply: 1)
1679. qPCR lacks reproduceability - perfect runs, no reproduceability, no explaination, desperate... (reply: 5)
1680. Using BLAST to check primers - (reply: 17)