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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1651. heating rna before rt reaction? - (reply: 1)
1652. relative gene expression with multiple reference genes - data analysis (reply: 1)
1653. qRT-PCR no of cycles?? - (reply: 6)
1654. Is there any setting to avoid same base runs in Primer-BLAST? - (reply: 3)
1655. Which techniques should i do? - Which techniques should i do? (reply: 2)
1656. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
1657. what primers should I use for DNA sequencing? please help - (reply: 2)
1658. Standard curve for RT-PCR - (reply: 1)
1659. Problem amplifying plasmid - (reply: 1)
1660. What Factors Affect Ct values of a gene? - (reply: 1)
1661. Sybr Green I in real-time PCR reaction - (reply: 3)
1662. Primers annealing temperatures - (reply: 8)
1663. Difficulties cloning by PCR - (reply: 5)
1664. PCR Result Explanation (I needed you help) - Refer to the result, what is indicate A? (reply: 4)
1665. rna storage at -20 - (reply: 2)
1666. miScript (Qiagen) - RT of both miRNA and mRNA - does it work? (reply: 1)
1667. high Cp value PCR - (reply: 2)
1668. real time PCR data presentation - presentation of delta CT and copy number (reply: 2)
1669. Synthesize own Oligo-dT primers - (reply: 6)
1670. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
1671. Cell-cycle independant qRT-PCR internal control - anyone has experience doing this? (reply: 1)
1672. dNTP or Deoxynucleotide solution Mix - (reply: 1)
1673. Smearing/ no amplification in PCR - (reply: 3)
1674. qRTPCR (TaqMan) Data Representation - (reply: 1)
1675. mRNA versus protein expression. Why inverse correlation? - (reply: 3)
1676. Lucicferase activity and endogenous transcription - (reply: 7)
1677. need help on primer calcuation soon - (reply: 1)
1678. Heart ache in real time - (reply: 1)
1679. HEX vs. Cy-5 - (reply: 3)
1680. Direct PCR (PCR without DNA extraction) - (reply: 2)