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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1651. 2 bands or smear? 2-step RT-PCR - (reply: 1)
1652. 23S rRNA Primer - need primer sequence (reply: 1)
1653. Lowered extension temps with Phusion - (reply: 7)
1654. RT-PCR - problems with TaqMan PCR (reply: 2)
1655. qRT-PCR calculations - Averaging across biological replicates (reply: 2)
1656. Two annealing temps? - (reply: 4)
1657. Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
1658. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
1659. Small product amplification problems - (reply: 4)
1660. ROX and Rotorgene - using a mix with reference dye in Rotorgene (reply: 2)
1661. Freezing primers+SYBR green - (reply: 2)
1662. Tagged Primers - (reply: 5)
1663. no pcr amplification product - (reply: 9)
1664. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
1665. less steep curve and high Ct value! - (reply: 2)
1666. Question about site directed mutagenesis - (reply: 1)
1667. Amplfication of fungal DNA - (reply: 8)
1668. PCR problem - (reply: 4)
1669. mRNA level in transgene cell line or knocking down cell line - (reply: 3)
1670. differently behavioring replicates in real-time PCR - (reply: 1)
1671. Multiplex PCR - (reply: 1)
1672. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
1673. optimal template dna concentrations - (reply: 1)
1674. reconstitution of primers - (reply: 2)
1675. Mysterious PCR Contamination - (reply: 13)
1676. RT-PCR Help - Protocol provided (reply: 4)
1677. Endogenous control in bacterial RT-qPCR - (reply: 4)
1678. Mutagenesis PCR and undesired amplification - (reply: 2)
1679. PCR-how to set 'Extension: ramping from 55 to 72 for 5min' - (reply: 4)
1680. primer dimer - (reply: 2)