Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
811. Principles of overlap PCR - (reply: 7)
812. Help designing primers with restriction sites - (reply: 3)
813. DNA sequence data came back with lots of wrong bases - (reply: 5)
814. Why primers shouldn't have Tm difference greater than 5C? - (reply: 5)
815. Abnormal amplification curve - (reply: 1)
816. Problem with Semiquantitative PCR - (reply: 4)
817. false positive/cross reaction with TaqMan - (reply: 1)
818. Problem w/ Genotyping Cre Only - (reply: 3)
819. ROX reference dye for quantitative RT-PCR - (reply: 1)
820. PCR problem - (reply: 9)
821. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
822. why PCR product smeared? - (reply: 2)
823. Tm calculation for primers with RE sites and overhangs - (reply: 4)
824. silincing box, no amplification - (reply: 3)
825. Comparative semiquantitative RT-PCR - (reply: 2)
826. Bands in my PCR Controls - (reply: 3)
827. RTPCR - housekeeping genes: can I use them more intelligently? - (reply: 4)
828. real time qRT-PCR and low expression gene - (reply: 2)
829. In a pcr why doesnt the DNA reneal during annealing process ? - (reply: 2)
830. check points while preparing for sequencing - (reply: 10)
831. Disagreement between triplicate results in Q -PCR - (reply: 5)
832. 40 cT values any idea how to lower it? - (reply: 1)
833. Trizol and Protein extraction - (reply: 2)
834. RT-PCR-CELL NUMBER - (reply: 2)
835. Real time pcr primers JunB, Foxh1, Klf4, PRMT1, PRMT4 and PRMT5 needed! - (reply: 2)
836. RNA extraction and preparation - RTqPCR - (reply: 3)
837. Mysterious -NRT control - (reply: 4)
838. PYO pcr stopped working. - (reply: 1)
839. Bands seen with qPCR missing in regular PCR - (reply: 5)
840. PCR product one day, none the next day - (reply: 4)