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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
811. Nuclear or Cytoplasmic RNA Purification - (reply: 1)
812. >100% qPCR efficiency - (reply: 2)
813. Why does freezing/thawing DNA samples first improve PCR results? - (reply: 8)
814. PCR Primers - (reply: 2)
815. Cleaning up RT-PCR product before sequencing - (reply: 1)
816. reference gene - (reply: 2)
817. Improving qRT-PCR detection limits - (reply: 2)
818. What to use for Standard Curve - (reply: 2)
819. gPCR - (reply: 1)
820. RT-PCR cDNA synthesis - (reply: 6)
821. problems amplifying from cDNA - (reply: 4)
822. Real-time PCR about Incorrect setup of the data collection stage - (reply: 1)
823. Primers... - (reply: 1)
824. 18s as endogenous control for Oligo dT RT - Can we use 18s ? (reply: 2)
825. excel and propagation of error, qpcr - (reply: 1)
826. Reverse transcription (cDNA synthesis) curiosity and confusion - Does the amount of RNA have to be doubled if you double everything els (reply: 4)
827. primer with higher melting temperature - (reply: 2)
828. Omitting Points in Standard Curve - (reply: 2)
829. Triage of miRNA targets by qPCR - (reply: 1)
830. Smallest reaction volume with Roche Lightcycler 480 96-well plate format - (reply: 2)
831. qPCR primer design - possible off-target (reply: 1)
832. Primer dimer formation and real-time PCR - (reply: 7)
833. 5' race - (reply: 2)
834. Identify PCR products - (reply: 3)
835. Maximum size of overhangs in PCR - (reply: 6)
836. water-droplets in my cycler !!! - experienced such thing ??? (reply: 9)
837. a second small peak to the right to my pricipal peak!!! - (reply: 1)
838. qPCR virgin, What do I do first? - How to set up experiment (reply: 1)
839. Colony PCR doesn't work anymore - (reply: 5)
840. Question about TM and Annealing temperatures - (reply: 4)