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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
811. Housekeeping gene for COS-1 cells - (reply: 1)
812. PCR, neg control & no. of cycles - (reply: 2)
813. How do I insert a DNA sequence into a multiple cloning site and add restriction - (reply: 1)
814. efficiency calculation from non-10 fold dilutions - (reply: 1)
815. whole plasmid pcr? - (reply: 6)
816. Proper storage of isolated white blood cell prior to RNA isolation - (reply: 3)
817. Genomic pseudogenes?!? - (reply: 6)
818. Strange amplification plot - (reply: 4)
819. Conventional PCR of different dilutions - (reply: 1)
820. primer resuspension - (reply: 2)
821. Principles of overlap PCR - (reply: 7)
822. Help designing primers with restriction sites - (reply: 3)
823. DNA sequence data came back with lots of wrong bases - (reply: 5)
824. Why primers shouldn't have Tm difference greater than 5C? - (reply: 5)
825. Abnormal amplification curve - (reply: 1)
826. Problem with Semiquantitative PCR - (reply: 4)
827. false positive/cross reaction with TaqMan - (reply: 1)
828. Problem w/ Genotyping Cre Only - (reply: 3)
829. ROX reference dye for quantitative RT-PCR - (reply: 1)
830. PCR problem - (reply: 9)
831. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
832. why PCR product smeared? - (reply: 2)
833. Tm calculation for primers with RE sites and overhangs - (reply: 4)
834. silincing box, no amplification - (reply: 3)
835. Comparative semiquantitative RT-PCR - (reply: 2)
836. Bands in my PCR Controls - (reply: 3)
837. RTPCR - housekeeping genes: can I use them more intelligently? - (reply: 4)
838. real time qRT-PCR and low expression gene - (reply: 2)
839. In a pcr why doesnt the DNA reneal during annealing process ? - (reply: 2)
840. check points while preparing for sequencing - (reply: 10)