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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
811. Primers... - (reply: 1)
812. 18s as endogenous control for Oligo dT RT - Can we use 18s ? (reply: 2)
813. excel and propagation of error, qpcr - (reply: 1)
814. Reverse transcription (cDNA synthesis) curiosity and confusion - Does the amount of RNA have to be doubled if you double everything els (reply: 4)
815. primer with higher melting temperature - (reply: 2)
816. Omitting Points in Standard Curve - (reply: 2)
817. Triage of miRNA targets by qPCR - (reply: 1)
818. Smallest reaction volume with Roche Lightcycler 480 96-well plate format - (reply: 2)
819. qPCR primer design - possible off-target (reply: 1)
820. Primer dimer formation and real-time PCR - (reply: 7)
821. 5' race - (reply: 2)
822. Identify PCR products - (reply: 3)
823. Maximum size of overhangs in PCR - (reply: 6)
824. water-droplets in my cycler !!! - experienced such thing ??? (reply: 9)
825. a second small peak to the right to my pricipal peak!!! - (reply: 1)
826. qPCR virgin, What do I do first? - How to set up experiment (reply: 1)
827. Colony PCR doesn't work anymore - (reply: 5)
828. Question about TM and Annealing temperatures - (reply: 4)
829. RT-PCR t-test, and ANOVA - (reply: 1)
830. PCR basics - I'm new to all things lab and need some help! (reply: 3)
831. the red dye in trizol? - (reply: 2)
832. Is it acceptable that qpcr reaction doesn't reach plateau? - (reply: 3)
833. What are exogeneous and endogeneous controls? - (reply: 4)
834. quantitate wild type and mutant mRNA - (reply: 1)
835. Can very low RNA concentration cause Ct bias..? - (reply: 3)
836. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
837. iScript BioRad cDNA synthesis yield - (reply: 1)
838. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
839. intron spanning primers for non model organisms - (reply: 2)
840. Using DNA as a reference in qRT-PCR - (reply: 1)