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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
811. qPCR result interpretation - when your 'relative' is 0?? - (reply: 2)
812. why is real time pcr curve like this? - (reply: 3)
813. Housekeeping gene for COS-1 cells - (reply: 1)
814. PCR, neg control & no. of cycles - (reply: 2)
815. How do I insert a DNA sequence into a multiple cloning site and add restriction - (reply: 1)
816. efficiency calculation from non-10 fold dilutions - (reply: 1)
817. whole plasmid pcr? - (reply: 6)
818. Proper storage of isolated white blood cell prior to RNA isolation - (reply: 3)
819. Genomic pseudogenes?!? - (reply: 6)
820. Strange amplification plot - (reply: 4)
821. Conventional PCR of different dilutions - (reply: 1)
822. primer resuspension - (reply: 2)
823. Principles of overlap PCR - (reply: 7)
824. Help designing primers with restriction sites - (reply: 3)
825. DNA sequence data came back with lots of wrong bases - (reply: 5)
826. Why primers shouldn't have Tm difference greater than 5C? - (reply: 5)
827. Abnormal amplification curve - (reply: 1)
828. Problem with Semiquantitative PCR - (reply: 4)
829. false positive/cross reaction with TaqMan - (reply: 1)
830. Problem w/ Genotyping Cre Only - (reply: 3)
831. ROX reference dye for quantitative RT-PCR - (reply: 1)
832. PCR problem - (reply: 9)
833. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
834. why PCR product smeared? - (reply: 2)
835. Tm calculation for primers with RE sites and overhangs - (reply: 4)
836. silincing box, no amplification - (reply: 3)
837. Comparative semiquantitative RT-PCR - (reply: 2)
838. Bands in my PCR Controls - (reply: 3)
839. RTPCR - housekeeping genes: can I use them more intelligently? - (reply: 4)
840. real time qRT-PCR and low expression gene - (reply: 2)