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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
811. DNA sequence data came back with lots of wrong bases - (reply: 5)
812. Why primers shouldn't have Tm difference greater than 5C? - (reply: 5)
813. Abnormal amplification curve - (reply: 1)
814. Problem with Semiquantitative PCR - (reply: 4)
815. false positive/cross reaction with TaqMan - (reply: 1)
816. Problem w/ Genotyping Cre Only - (reply: 3)
817. ROX reference dye for quantitative RT-PCR - (reply: 1)
818. PCR problem - (reply: 9)
819. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
820. why PCR product smeared? - (reply: 2)
821. Tm calculation for primers with RE sites and overhangs - (reply: 4)
822. silincing box, no amplification - (reply: 3)
823. Comparative semiquantitative RT-PCR - (reply: 2)
824. Bands in my PCR Controls - (reply: 3)
825. RTPCR - housekeeping genes: can I use them more intelligently? - (reply: 4)
826. real time qRT-PCR and low expression gene - (reply: 2)
827. In a pcr why doesnt the DNA reneal during annealing process ? - (reply: 2)
828. check points while preparing for sequencing - (reply: 10)
829. Disagreement between triplicate results in Q -PCR - (reply: 5)
830. 40 cT values any idea how to lower it? - (reply: 1)
831. Trizol and Protein extraction - (reply: 2)
832. RT-PCR-CELL NUMBER - (reply: 2)
833. Real time pcr primers JunB, Foxh1, Klf4, PRMT1, PRMT4 and PRMT5 needed! - (reply: 2)
834. RNA extraction and preparation - RTqPCR - (reply: 3)
835. Mysterious -NRT control - (reply: 4)
836. PYO pcr stopped working. - (reply: 1)
837. Bands seen with qPCR missing in regular PCR - (reply: 5)
838. PCR product one day, none the next day - (reply: 4)
839. PCR using CDNA as template - (reply: 2)
840. why cant get the band? - (reply: 4)