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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
811. Total RNA estimation - (reply: 2)
812. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
813. pcr contamination - (reply: 4)
814. Large Non-specfic bands in PCR - (reply: 3)
815. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
816. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
817. Primer dilution --> problem.. - (reply: 16)
818. no bands after pcr - even after we have change most of the reagents (reply: 2)
819. High resolution melting to gene scanning - (reply: 1)
820. Help! Smear in Real-Time PCR Product - (reply: 1)
821. Is one reference gene ever appropriate? - (reply: 3)
822. Sybr green RT-qPCR primer - (reply: 1)
823. Sybr green RT-qPCR primer - (reply: 1)
824. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
825. Oligo Question - (reply: 1)
826. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
827. real time rt PCR - bloodyurine - inhibition - (reply: 1)
828. No Amplification in PCR - (reply: 4)
829. DNase treatment after RNA extraction using RNeasy kit of qiagen - DNaes treatment after RNA extraction (reply: 2)
830. Real time PCR and percentage loss - (reply: 1)
831. Problem with smear in PCR with certain templates - (reply: 4)
832. running multiple qPCR with common housekeeping control - (reply: 3)
833. Problems with PCR in general - (reply: 1)
834. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 2)
835. Control inconsistence in Q-PCR - (reply: 1)
836. how to overcome primer contamination - need protocol (reply: 11)
837. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
838. sequencing problem - (reply: 1)
839. High Resolution Melting (HRM) Problem - (reply: 4)
840. Consistancy in qPCR data - (reply: 5)