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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
811. Difficult sequences in PCR - (reply: 2)
812. qPCR result interpretation - when your 'relative' is 0?? - (reply: 2)
813. why is real time pcr curve like this? - (reply: 3)
814. Housekeeping gene for COS-1 cells - (reply: 1)
815. PCR, neg control & no. of cycles - (reply: 2)
816. How do I insert a DNA sequence into a multiple cloning site and add restriction - (reply: 1)
817. efficiency calculation from non-10 fold dilutions - (reply: 1)
818. whole plasmid pcr? - (reply: 6)
819. Proper storage of isolated white blood cell prior to RNA isolation - (reply: 3)
820. Genomic pseudogenes?!? - (reply: 6)
821. Strange amplification plot - (reply: 4)
822. Conventional PCR of different dilutions - (reply: 1)
823. primer resuspension - (reply: 2)
824. Principles of overlap PCR - (reply: 7)
825. Help designing primers with restriction sites - (reply: 3)
826. DNA sequence data came back with lots of wrong bases - (reply: 5)
827. Why primers shouldn't have Tm difference greater than 5C? - (reply: 5)
828. Abnormal amplification curve - (reply: 1)
829. Problem with Semiquantitative PCR - (reply: 4)
830. false positive/cross reaction with TaqMan - (reply: 1)
831. Problem w/ Genotyping Cre Only - (reply: 3)
832. ROX reference dye for quantitative RT-PCR - (reply: 1)
833. PCR problem - (reply: 9)
834. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
835. why PCR product smeared? - (reply: 2)
836. Tm calculation for primers with RE sites and overhangs - (reply: 4)
837. silincing box, no amplification - (reply: 3)
838. Comparative semiquantitative RT-PCR - (reply: 2)
839. Bands in my PCR Controls - (reply: 3)
840. RTPCR - housekeeping genes: can I use them more intelligently? - (reply: 4)