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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
811. PCR basics - I'm new to all things lab and need some help! (reply: 3)
812. the red dye in trizol? - (reply: 2)
813. Is it acceptable that qpcr reaction doesn't reach plateau? - (reply: 3)
814. What are exogeneous and endogeneous controls? - (reply: 4)
815. quantitate wild type and mutant mRNA - (reply: 1)
816. Can very low RNA concentration cause Ct bias..? - (reply: 3)
817. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
818. iScript BioRad cDNA synthesis yield - (reply: 1)
819. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
820. intron spanning primers for non model organisms - (reply: 2)
821. Using DNA as a reference in qRT-PCR - (reply: 1)
822. minimum replicate for qpcr? - (reply: 15)
823. Primer design - (reply: 2)
824. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
825. qPCR amplification interference fixed with freezing - (reply: 6)
826. Suggest me a mastermix for conventinal PCR... - (reply: 11)
827. reaction efficiency - (reply: 4)
828. software for quantifing PCR product band - (reply: 1)
829. Thermal cycler - (reply: 2)
830. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
831. qPCR volume - Problem with volume difference and results (reply: 1)
832. RT-PCR - (reply: 9)
833. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
834. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
835. cDNA synthesis - (reply: 6)
836. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
837. Primer seq - (reply: 2)
838. Selection of housekeeping gene in Mycobacterium - (reply: 1)
839. Do I need to pool the base line samples together - (reply: 2)
840. PCR product size - (reply: 6)