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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
811. Real-Time efficiencies and the pfaffl method - (reply: 1)
812. should I design primers spanning both intron and exon - (reply: 2)
813. RT-PCR - (reply: 1)
814. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
815. PCR with a very long and a short primer - (reply: 5)
816. qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
817. How to find that my RNA is not contaminated with gDNA, before running Real-Time - (reply: 5)
818. Dilution of 18s. How much? - (reply: 1)
819. Multiplex PCR does not work - (reply: 3)
820. Alternative to Sybr-green? - (reply: 12)
821. Multiple internal controls in using qPCR to measure gene duplication - (reply: 1)
822. ct value of neg - (reply: 1)
823. Help: Linear Amplification Using Hydrolysis Probes - (reply: 3)
824. Standard curve - (reply: 3)
825. Discuss about COLD-PCR - (reply: 3)
826. Comparison between two primers pairs? Is it possible? - (reply: 3)
827. Rt-PCR primer design - (reply: 7)
828. Length of non-binding sequence in primers with RE site - (reply: 6)
829. qPCR Calc Help - (reply: 6)
830. ABI Prism 7700 software - (reply: 1)
831. Difficult sequences in PCR - (reply: 2)
832. qPCR result interpretation - when your 'relative' is 0?? - (reply: 2)
833. why is real time pcr curve like this? - (reply: 3)
834. Housekeeping gene for COS-1 cells - (reply: 1)
835. PCR, neg control & no. of cycles - (reply: 2)
836. How do I insert a DNA sequence into a multiple cloning site and add restriction - (reply: 1)
837. efficiency calculation from non-10 fold dilutions - (reply: 1)
838. whole plasmid pcr? - (reply: 6)
839. Proper storage of isolated white blood cell prior to RNA isolation - (reply: 3)
840. Genomic pseudogenes?!? - (reply: 6)