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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
811. the red dye in trizol? - (reply: 2)
812. Is it acceptable that qpcr reaction doesn't reach plateau? - (reply: 3)
813. What are exogeneous and endogeneous controls? - (reply: 4)
814. quantitate wild type and mutant mRNA - (reply: 1)
815. Can very low RNA concentration cause Ct bias..? - (reply: 3)
816. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
817. iScript BioRad cDNA synthesis yield - (reply: 1)
818. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
819. intron spanning primers for non model organisms - (reply: 2)
820. Using DNA as a reference in qRT-PCR - (reply: 1)
821. minimum replicate for qpcr? - (reply: 15)
822. Primer design - (reply: 2)
823. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
824. qPCR amplification interference fixed with freezing - (reply: 6)
825. Suggest me a mastermix for conventinal PCR... - (reply: 11)
826. reaction efficiency - (reply: 4)
827. software for quantifing PCR product band - (reply: 1)
828. Thermal cycler - (reply: 2)
829. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
830. qPCR volume - Problem with volume difference and results (reply: 1)
831. RT-PCR - (reply: 9)
832. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
833. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
834. cDNA synthesis - (reply: 6)
835. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
836. Primer seq - (reply: 2)
837. Selection of housekeeping gene in Mycobacterium - (reply: 1)
838. Do I need to pool the base line samples together - (reply: 2)
839. PCR product size - (reply: 6)
840. amplification plot raises to early - What is the explanation for this problem? (reply: 1)