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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
811. Adding loading/tracking dye to PCR mix? - (reply: 10)
812. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
813. internal control in RT-PCR - inquiry (reply: 2)
814. Melting Temp of Standards Changed? - (reply: 3)
815. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
816. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
817. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
818. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
819. Gene on negative strand - two eductional questions. - (reply: 3)
820. Newbie- choose DNA template - (reply: 1)
821. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
822. Total RNA estimation - (reply: 2)
823. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
824. pcr contamination - (reply: 4)
825. Large Non-specfic bands in PCR - (reply: 3)
826. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
827. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
828. Primer dilution --> problem.. - (reply: 16)
829. no bands after pcr - even after we have change most of the reagents (reply: 2)
830. High resolution melting to gene scanning - (reply: 1)
831. Help! Smear in Real-Time PCR Product - (reply: 1)
832. Is one reference gene ever appropriate? - (reply: 3)
833. Sybr green RT-qPCR primer - (reply: 1)
834. Sybr green RT-qPCR primer - (reply: 1)
835. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
836. Oligo Question - (reply: 1)
837. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
838. real time rt PCR - bloodyurine - inhibition - (reply: 1)
839. No Amplification in PCR - (reply: 4)
840. DNase treatment after RNA extraction using RNeasy kit of qiagen - DNaes treatment after RNA extraction (reply: 2)