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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
811. PCR is amplifying non-specific fragment - (reply: 6)
812. Anybody know about temperature sensitivity of the AB TaqMan universal Mastermix - (reply: 4)
813. Measuring of cDNA bands density - (reply: 3)
814. real time RT-PCR problems - (reply: 2)
815. Maximum Annealing-Temperature for Real-Time? - (reply: 3)
816. Real-Time efficiencies and the pfaffl method - (reply: 1)
817. should I design primers spanning both intron and exon - (reply: 2)
818. RT-PCR - (reply: 1)
819. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
820. PCR with a very long and a short primer - (reply: 5)
821. qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
822. How to find that my RNA is not contaminated with gDNA, before running Real-Time - (reply: 5)
823. Dilution of 18s. How much? - (reply: 1)
824. Multiplex PCR does not work - (reply: 3)
825. Alternative to Sybr-green? - (reply: 12)
826. Multiple internal controls in using qPCR to measure gene duplication - (reply: 1)
827. ct value of neg - (reply: 1)
828. Help: Linear Amplification Using Hydrolysis Probes - (reply: 3)
829. Standard curve - (reply: 3)
830. Discuss about COLD-PCR - (reply: 3)
831. Comparison between two primers pairs? Is it possible? - (reply: 3)
832. Rt-PCR primer design - (reply: 7)
833. Length of non-binding sequence in primers with RE site - (reply: 6)
834. qPCR Calc Help - (reply: 6)
835. ABI Prism 7700 software - (reply: 1)
836. Difficult sequences in PCR - (reply: 2)
837. qPCR result interpretation - when your 'relative' is 0?? - (reply: 2)
838. why is real time pcr curve like this? - (reply: 3)
839. Housekeeping gene for COS-1 cells - (reply: 1)
840. PCR, neg control & no. of cycles - (reply: 2)