Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
811. Why primers shouldn't have Tm difference greater than 5C? - (reply: 5)
812. Abnormal amplification curve - (reply: 1)
813. Problem with Semiquantitative PCR - (reply: 4)
814. false positive/cross reaction with TaqMan - (reply: 1)
815. Problem w/ Genotyping Cre Only - (reply: 3)
816. ROX reference dye for quantitative RT-PCR - (reply: 1)
817. PCR problem - (reply: 9)
818. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
819. why PCR product smeared? - (reply: 2)
820. Tm calculation for primers with RE sites and overhangs - (reply: 4)
821. silincing box, no amplification - (reply: 3)
822. Comparative semiquantitative RT-PCR - (reply: 2)
823. Bands in my PCR Controls - (reply: 3)
824. RTPCR - housekeeping genes: can I use them more intelligently? - (reply: 4)
825. real time qRT-PCR and low expression gene - (reply: 2)
826. In a pcr why doesnt the DNA reneal during annealing process ? - (reply: 2)
827. check points while preparing for sequencing - (reply: 10)
828. Disagreement between triplicate results in Q -PCR - (reply: 5)
829. 40 cT values any idea how to lower it? - (reply: 1)
830. Trizol and Protein extraction - (reply: 2)
831. RT-PCR-CELL NUMBER - (reply: 2)
832. Real time pcr primers JunB, Foxh1, Klf4, PRMT1, PRMT4 and PRMT5 needed! - (reply: 2)
833. RNA extraction and preparation - RTqPCR - (reply: 3)
834. Mysterious -NRT control - (reply: 4)
835. PYO pcr stopped working. - (reply: 1)
836. Bands seen with qPCR missing in regular PCR - (reply: 5)
837. PCR product one day, none the next day - (reply: 4)
838. PCR using CDNA as template - (reply: 2)
839. why cant get the band? - (reply: 4)
840. CT value too small - (reply: 1)