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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1861. Shrimp nuclease to eliminate genomic DNA - (reply: 7)
1862. How do you manually determine Ct value? - (reply: 1)
1863. Running gel after RT-PCR - (reply: 5)
1864. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
1865. Etbr staining methods - (reply: 6)
1866. PCR problems.. - problems with conventional PCR (reply: 3)
1867. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
1868. gDNA or cDNA amplification in RT-PCR - (reply: 3)
1869. Dnase digestion and PCR - (reply: 4)
1870. Understanding qPCR and gene expression changes - (reply: 10)
1871. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
1872. Reverse Transcription - (reply: 3)
1873. Plasmid as standard - Plasmid calculation (reply: 6)
1874. what Ct values are considered ideal? too high? - (reply: 1)
1875. Need an UV260 RT-PCR instrument - (reply: 1)
1876. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
1877. PCR produces products of wrong size - (reply: 4)
1878. PCR with pfu and degenerate primers (?) - (reply: 7)
1879. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
1880. Serious issues with RT-PCR - (reply: 7)
1881. relative quantitation problem plz help - (reply: 4)
1882. No beta actin results at all??? - (reply: 1)
1883. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
1884. dNTP question - (reply: 3)
1885. Taqman rtPCR primer and probe design - (reply: 3)
1886. Help with Real Time PCR Well To Well Variation - (reply: 10)
1887. dna for real time - (reply: 3)
1888. Mycobacterial housekeeping genes - (reply: 1)
1889. Expression comparision using Cp values - (reply: 1)
1890. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)