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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1861. Dnase digestion and PCR - (reply: 4)
1862. Understanding qPCR and gene expression changes - (reply: 10)
1863. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
1864. Reverse Transcription - (reply: 3)
1865. Plasmid as standard - Plasmid calculation (reply: 6)
1866. what Ct values are considered ideal? too high? - (reply: 1)
1867. Need an UV260 RT-PCR instrument - (reply: 1)
1868. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
1869. PCR produces products of wrong size - (reply: 4)
1870. PCR with pfu and degenerate primers (?) - (reply: 7)
1871. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
1872. Serious issues with RT-PCR - (reply: 7)
1873. relative quantitation problem plz help - (reply: 4)
1874. No beta actin results at all??? - (reply: 1)
1875. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
1876. dNTP question - (reply: 3)
1877. Taqman rtPCR primer and probe design - (reply: 3)
1878. Help with Real Time PCR Well To Well Variation - (reply: 10)
1879. dna for real time - (reply: 3)
1880. Mycobacterial housekeeping genes - (reply: 1)
1881. Expression comparision using Cp values - (reply: 1)
1882. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
1883. Which to use for standard curve? - (reply: 2)
1884. How do you calibrate between runs? - (reply: 3)
1885. Houskeeping Gene not steady - (reply: 7)
1886. real time PCR - (reply: 3)
1887. Real-Time RT-PCR one-step and two step issue - (reply: 13)
1888. Plateau Phase - Taqman not reaching plateau (reply: 1)
1889. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
1890. maximum amplicon size - 550bp too long??? (reply: 5)