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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1861. cDNA spectrophotometric quantification - (reply: 8)
1862. Standard curve standardization - variation between runs (reply: 4)
1863. How to recognize primer-dimers? - from a melting curve??... (reply: 4)
1864. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
1865. real time curve shifted by ~10 cycles - higher Cts than I used to (reply: 2)
1866. low gc primers - help with pcr using low gc primers (reply: 6)
1867. Exon-Exon Junction ? - (reply: 3)
1868. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
1869. Primers and annealing temperature - (reply: 2)
1870. Bacteria gene expression - (reply: 2)
1871. Copy number calculations in real time PCR - (reply: 2)
1872. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
1873. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
1874. RT-PCR Negative controls - (reply: 1)
1875. Problems with qRT-PCR - (reply: 3)
1876. PCR with 60 bp primers - results in no product (reply: 6)
1877. Shrimp nuclease to eliminate genomic DNA - (reply: 7)
1878. How do you manually determine Ct value? - (reply: 1)
1879. Running gel after RT-PCR - (reply: 5)
1880. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
1881. Etbr staining methods - (reply: 6)
1882. PCR problems.. - problems with conventional PCR (reply: 3)
1883. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
1884. gDNA or cDNA amplification in RT-PCR - (reply: 3)
1885. Dnase digestion and PCR - (reply: 4)
1886. Understanding qPCR and gene expression changes - (reply: 10)
1887. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
1888. Reverse Transcription - (reply: 3)
1889. Plasmid as standard - Plasmid calculation (reply: 6)
1890. what Ct values are considered ideal? too high? - (reply: 1)