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New Forum Archives (2009-): :
1861. Gene-specific RT-PCR - (reply: 2)
1863. selection of internal control gene - (reply: 2)
1865. primer design - primer design question (reply: 2)
1867. qPCR - Standard curves (reply: 3)
1869. Standard curve standardization - variation between runs (reply: 4)
1871. real time PCR primer designing problems,again - is NCBI's primer blast believable enough？ (reply: 3)
1873. low gc primers - help with pcr using low gc primers (reply: 6)
1875. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
1877. Bacteria gene expression - (reply: 2)
1879. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
1881. RT-PCR Negative controls - (reply: 1)
1883. PCR with 60 bp primers - results in no product (reply: 6)
1885. How do you manually determine Ct value? - (reply: 1)
1887. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
1889. PCR problems.. - problems with conventional PCR (reply: 3)
1863. selection of internal control gene - (reply: 2)
1865. primer design - primer design question (reply: 2)
1867. qPCR - Standard curves (reply: 3)
1869. Standard curve standardization - variation between runs (reply: 4)
1871. real time PCR primer designing problems,again - is NCBI's primer blast believable enough？ (reply: 3)
1873. low gc primers - help with pcr using low gc primers (reply: 6)
1875. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
1877. Bacteria gene expression - (reply: 2)
1879. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
1881. RT-PCR Negative controls - (reply: 1)
1883. PCR with 60 bp primers - results in no product (reply: 6)
1885. How do you manually determine Ct value? - (reply: 1)
1887. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
1889. PCR problems.. - problems with conventional PCR (reply: 3)