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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1861. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
1862. gDNA or cDNA amplification in RT-PCR - (reply: 3)
1863. Dnase digestion and PCR - (reply: 4)
1864. Understanding qPCR and gene expression changes - (reply: 10)
1865. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
1866. Reverse Transcription - (reply: 3)
1867. Plasmid as standard - Plasmid calculation (reply: 6)
1868. what Ct values are considered ideal? too high? - (reply: 1)
1869. Need an UV260 RT-PCR instrument - (reply: 1)
1870. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
1871. PCR produces products of wrong size - (reply: 4)
1872. PCR with pfu and degenerate primers (?) - (reply: 7)
1873. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
1874. Serious issues with RT-PCR - (reply: 7)
1875. relative quantitation problem plz help - (reply: 4)
1876. No beta actin results at all??? - (reply: 1)
1877. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
1878. dNTP question - (reply: 3)
1879. Taqman rtPCR primer and probe design - (reply: 3)
1880. Help with Real Time PCR Well To Well Variation - (reply: 10)
1881. dna for real time - (reply: 3)
1882. Mycobacterial housekeeping genes - (reply: 1)
1883. Expression comparision using Cp values - (reply: 1)
1884. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
1885. Which to use for standard curve? - (reply: 2)
1886. How do you calibrate between runs? - (reply: 3)
1887. Houskeeping Gene not steady - (reply: 7)
1888. real time PCR - (reply: 3)
1889. Real-Time RT-PCR one-step and two step issue - (reply: 13)
1890. Plateau Phase - Taqman not reaching plateau (reply: 1)