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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1861. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
1862. real time curve shifted by ~10 cycles - higher Cts than I used to (reply: 2)
1863. low gc primers - help with pcr using low gc primers (reply: 6)
1864. Exon-Exon Junction ? - (reply: 3)
1865. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
1866. Primers and annealing temperature - (reply: 2)
1867. Bacteria gene expression - (reply: 2)
1868. Copy number calculations in real time PCR - (reply: 2)
1869. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
1870. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
1871. RT-PCR Negative controls - (reply: 1)
1872. Problems with qRT-PCR - (reply: 3)
1873. PCR with 60 bp primers - results in no product (reply: 6)
1874. Shrimp nuclease to eliminate genomic DNA - (reply: 7)
1875. How do you manually determine Ct value? - (reply: 1)
1876. Running gel after RT-PCR - (reply: 5)
1877. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
1878. Etbr staining methods - (reply: 6)
1879. PCR problems.. - problems with conventional PCR (reply: 3)
1880. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
1881. gDNA or cDNA amplification in RT-PCR - (reply: 3)
1882. Dnase digestion and PCR - (reply: 4)
1883. Understanding qPCR and gene expression changes - (reply: 10)
1884. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
1885. Reverse Transcription - (reply: 3)
1886. Plasmid as standard - Plasmid calculation (reply: 6)
1887. what Ct values are considered ideal? too high? - (reply: 1)
1888. Need an UV260 RT-PCR instrument - (reply: 1)
1889. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
1890. PCR produces products of wrong size - (reply: 4)