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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1861. Exon-Exon Junction ? - (reply: 3)
1862. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
1863. Primers and annealing temperature - (reply: 2)
1864. Bacteria gene expression - (reply: 2)
1865. Copy number calculations in real time PCR - (reply: 2)
1866. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
1867. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
1868. RT-PCR Negative controls - (reply: 1)
1869. Problems with qRT-PCR - (reply: 3)
1870. PCR with 60 bp primers - results in no product (reply: 6)
1871. Shrimp nuclease to eliminate genomic DNA - (reply: 7)
1872. How do you manually determine Ct value? - (reply: 1)
1873. Running gel after RT-PCR - (reply: 5)
1874. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
1875. Etbr staining methods - (reply: 6)
1876. PCR problems.. - problems with conventional PCR (reply: 3)
1877. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
1878. gDNA or cDNA amplification in RT-PCR - (reply: 3)
1879. Dnase digestion and PCR - (reply: 4)
1880. Understanding qPCR and gene expression changes - (reply: 10)
1881. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
1882. Reverse Transcription - (reply: 3)
1883. Plasmid as standard - Plasmid calculation (reply: 6)
1884. what Ct values are considered ideal? too high? - (reply: 1)
1885. Need an UV260 RT-PCR instrument - (reply: 1)
1886. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
1887. PCR produces products of wrong size - (reply: 4)
1888. PCR with pfu and degenerate primers (?) - (reply: 7)
1889. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
1890. Serious issues with RT-PCR - (reply: 7)