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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1471. Freezing primers+SYBR green - (reply: 2)
1472. Tagged Primers - (reply: 5)
1473. no pcr amplification product - (reply: 9)
1474. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
1475. less steep curve and high Ct value! - (reply: 2)
1476. Question about site directed mutagenesis - (reply: 1)
1477. Amplfication of fungal DNA - (reply: 8)
1478. PCR problem - (reply: 4)
1479. mRNA level in transgene cell line or knocking down cell line - (reply: 3)
1480. differently behavioring replicates in real-time PCR - (reply: 1)
1481. Multiplex PCR - (reply: 1)
1482. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
1483. optimal template dna concentrations - (reply: 1)
1484. reconstitution of primers - (reply: 2)
1485. Mysterious PCR Contamination - (reply: 13)
1486. RT-PCR Help - Protocol provided (reply: 4)
1487. Endogenous control in bacterial RT-qPCR - (reply: 4)
1488. Mutagenesis PCR and undesired amplification - (reply: 2)
1489. PCR-how to set 'Extension: ramping from 55 to 72 for 5min' - (reply: 4)
1490. primer dimer - (reply: 2)
1491. Genotyping conditioning-where to start? - (reply: 1)
1492. Marker migrates slower depending where is loaded - (reply: 2)
1493. PCR on degraded templates - (reply: 2)
1494. PCR help needed - (reply: 3)
1495. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
1496. Tm difference - (reply: 2)
1497. Genotyping - PCR not working (reply: 6)
1498. RT qPCR Newbie, Weird Results - (reply: 1)
1499. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
1500. light bands, dark smear, dark dimers - (reply: 2)