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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1471. Relative RT-PCR - (reply: 2)
1472. Relative real time RT PCR - (reply: 2)
1473. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
1474. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
1475. IQ5 and sybr/FAM - (reply: 3)
1476. a simple primer problem - (reply: 5)
1477. Failure to introduce mutations using Overlap PCR - (reply: 3)
1478. no or poor amplification - (reply: 1)
1479. Primers from 3'UTR - (reply: 2)
1480. Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
1481. Designing primers with ESTs - (reply: 2)
1482. Ct variation/Ct limit/rē values/efficiency errors - (reply: 2)
1483. Probe manufacturers, who makes best probes? - Where do you get your probes and are you happy with them? (reply: 3)
1484. Real Time vs Traditional PCR results - (reply: 1)
1485. The problems to identify mouse genotype with PCR - (reply: 1)
1486. non specific amplification - DNA amplification by PCR (reply: 1)
1487. RNA Extraction - Extraction of Yeast RNA from mice blood (reply: 1)
1488. Large Difference between Ct of 18s rRNA and Target Gene - (reply: 4)
1489. PCR has stopped working - (reply: 7)
1490. PCR negative control contamination - (reply: 8)
1491. Real-Time PCR - Gene expression (reply: 2)
1492. PCR efficiency in real timePCR - (reply: 5)
1493. Plate setup for qRT-PCR - Too many samples for one plate... (reply: 1)
1494. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
1495. QPCR analysis with various # of samples from 1 patient? - (reply: 1)
1496. Drastic Decrease in PCR product yield - (reply: 2)
1497. Contamination? - (reply: 1)
1498. multiplexing taqman miRNA reverse transcription - (reply: 2)
1499. ligation problem - (reply: 3)
1500. Analyzing qPCR results - (reply: 1)