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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1471. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
1472. gDNA pcr product as standard for absolute quantification? - (reply: 1)
1473. qPCR - no signal detected but plenty of product on gel - (reply: 3)
1474. Amplify cDNA for qRT-PCR? - (reply: 6)
1475. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
1476. QPCR problem! - (reply: 9)
1477. pcr trouble solved but I cant seem to understand why - (reply: 1)
1478. Primer design if sequence is unknown for organism - (reply: 5)
1479. Reference gene qPCR - (reply: 4)
1480. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
1481. Real time PCR info - (reply: 4)
1482. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
1483. standard curve - (reply: 2)
1484. PCR from BAC - PCR from BAC (reply: 1)
1485. Mean versus Mediat Ct Values - Which to use for statistical analsyis (reply: 1)
1486. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
1487. High Molecular weight artifact LA-PCR 2-step - Artifact or real band - higher than expected Mw amplified product (reply: 3)
1488. heating rna before rt reaction? - (reply: 1)
1489. relative gene expression with multiple reference genes - data analysis (reply: 1)
1490. qRT-PCR no of cycles?? - (reply: 6)
1491. Is there any setting to avoid same base runs in Primer-BLAST? - (reply: 3)
1492. Which techniques should i do? - Which techniques should i do? (reply: 2)
1493. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
1494. what primers should I use for DNA sequencing? please help - (reply: 2)
1495. Standard curve for RT-PCR - (reply: 1)
1496. Problem amplifying plasmid - (reply: 1)
1497. What Factors Affect Ct values of a gene? - (reply: 1)
1498. Sybr Green I in real-time PCR reaction - (reply: 3)
1499. Primers annealing temperatures - (reply: 8)
1500. Difficulties cloning by PCR - (reply: 5)