Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
781. Mutagenesis not working - (reply: 5)
782. I need an urgent help with q-pcr amplification plots - (reply: 11)
783. Primers - Primers (reply: 2)
784. Problems with the Specificity of the Primers - (reply: 2)
785. qPCR Data analysis - qBasePlus and GenEx (reply: 1)
786. confusion in real time PCR primers - Confusion in Real time pcr primers (reply: 3)
787. Degenerate primers PCR problem, Please help! - (reply: 2)
788. degenerated Taqman probes? - (reply: 1)
789. error message on qPCR mashine - (reply: 1)
790. Hotstart PCR and unspecific Amplification - (reply: 3)
791. Primer design help needed - (reply: 1)
792. Primers no longer work - (reply: 1)
793. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
794. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
795. colony PCR - (reply: 2)
796. copy number variation - (reply: 3)
797. How to deal with truncated mRNA or mRNA that has secondary structure - (reply: 2)
798. Very weird gel - I cannot explain these results (reply: 11)
799. well position effects (edge and corner) effects in qPCR - using Bio-Rad iCycler iQ (reply: 2)
800. Cytosolic Housekeeping gene - (reply: 1)
801. PCR reaction without template gives a product - (reply: 6)
802. PCR wrong product size - (reply: 6)
803. PCR very faint product - (reply: 1)
804. proteinase k inhibition/inactivation - method... (reply: 2)
805. What does melting temperature indicate and how to predict annealing temperature - (reply: 9)
806. 4 question for real time PCR - (reply: 5)
807. RNA quality - (reply: 3)
808. Superscript III - No second strand synthesis? - (reply: 1)
809. cDNA convertion to DNA - (reply: 4)
810. PCR didn't work - suspect the enzyme (reply: 2)