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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
781. negative values after GOI - reference gene ?? - (reply: 4)
782. RNA degradation during electrophoresis?? - (reply: 2)
783. Primer 3' mismatch - Strange experience (reply: 9)
784. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
785. Adding loading/tracking dye to PCR mix? - (reply: 10)
786. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
787. internal control in RT-PCR - inquiry (reply: 2)
788. Melting Temp of Standards Changed? - (reply: 3)
789. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
790. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
791. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
792. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
793. Gene on negative strand - two eductional questions. - (reply: 3)
794. Newbie- choose DNA template - (reply: 1)
795. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
796. Total RNA estimation - (reply: 2)
797. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
798. pcr contamination - (reply: 4)
799. Large Non-specfic bands in PCR - (reply: 3)
800. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
801. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
802. Primer dilution --> problem.. - (reply: 16)
803. no bands after pcr - even after we have change most of the reagents (reply: 2)
804. High resolution melting to gene scanning - (reply: 1)
805. Help! Smear in Real-Time PCR Product - (reply: 1)
806. Is one reference gene ever appropriate? - (reply: 3)
807. Sybr green RT-qPCR primer - (reply: 1)
808. Sybr green RT-qPCR primer - (reply: 1)
809. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
810. Oligo Question - (reply: 1)