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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
781. Why does freezing/thawing DNA samples first improve PCR results? - (reply: 8)
782. PCR Primers - (reply: 2)
783. Cleaning up RT-PCR product before sequencing - (reply: 1)
784. reference gene - (reply: 2)
785. Improving qRT-PCR detection limits - (reply: 2)
786. What to use for Standard Curve - (reply: 2)
787. gPCR - (reply: 1)
788. RT-PCR cDNA synthesis - (reply: 6)
789. problems amplifying from cDNA - (reply: 4)
790. Real-time PCR about Incorrect setup of the data collection stage - (reply: 1)
791. Primers... - (reply: 1)
792. 18s as endogenous control for Oligo dT RT - Can we use 18s ? (reply: 2)
793. excel and propagation of error, qpcr - (reply: 1)
794. Reverse transcription (cDNA synthesis) curiosity and confusion - Does the amount of RNA have to be doubled if you double everything els (reply: 4)
795. primer with higher melting temperature - (reply: 2)
796. Omitting Points in Standard Curve - (reply: 2)
797. Triage of miRNA targets by qPCR - (reply: 1)
798. Smallest reaction volume with Roche Lightcycler 480 96-well plate format - (reply: 2)
799. qPCR primer design - possible off-target (reply: 1)
800. Primer dimer formation and real-time PCR - (reply: 7)
801. 5' race - (reply: 2)
802. Identify PCR products - (reply: 3)
803. Maximum size of overhangs in PCR - (reply: 6)
804. water-droplets in my cycler !!! - experienced such thing ??? (reply: 9)
805. a second small peak to the right to my pricipal peak!!! - (reply: 1)
806. qPCR virgin, What do I do first? - How to set up experiment (reply: 1)
807. Colony PCR doesn't work anymore - (reply: 5)
808. Question about TM and Annealing temperatures - (reply: 4)
809. RT-PCR t-test, and ANOVA - (reply: 1)
810. PCR basics - I'm new to all things lab and need some help! (reply: 3)