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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
781. rityProbe Integrity in Mastermix during loading 96 well plate - (reply: 2)
782. cloning with nested pcr - (reply: 3)
783. how to find 16srRNA sequences? - (reply: 3)
784. RNA extraction from a 1mm brain tissue punch - uncertain about homogenisation - (reply: 2)
785. What is the maxium amount of glycerol can be added in a PCR reaction? - (reply: 3)
786. PCR is amplifying non-specific fragment - (reply: 6)
787. Anybody know about temperature sensitivity of the AB TaqMan universal Mastermix - (reply: 4)
788. Measuring of cDNA bands density - (reply: 3)
789. real time RT-PCR problems - (reply: 2)
790. Maximum Annealing-Temperature for Real-Time? - (reply: 3)
791. Real-Time efficiencies and the pfaffl method - (reply: 1)
792. should I design primers spanning both intron and exon - (reply: 2)
793. RT-PCR - (reply: 1)
794. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
795. PCR with a very long and a short primer - (reply: 5)
796. qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
797. How to find that my RNA is not contaminated with gDNA, before running Real-Time - (reply: 5)
798. Dilution of 18s. How much? - (reply: 1)
799. Multiplex PCR does not work - (reply: 3)
800. Alternative to Sybr-green? - (reply: 12)
801. Multiple internal controls in using qPCR to measure gene duplication - (reply: 1)
802. ct value of neg - (reply: 1)
803. Help: Linear Amplification Using Hydrolysis Probes - (reply: 3)
804. Standard curve - (reply: 3)
805. Discuss about COLD-PCR - (reply: 3)
806. Comparison between two primers pairs? Is it possible? - (reply: 3)
807. Rt-PCR primer design - (reply: 7)
808. Length of non-binding sequence in primers with RE site - (reply: 6)
809. qPCR Calc Help - (reply: 6)
810. ABI Prism 7700 software - (reply: 1)