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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
781. clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
782. No gene amplification, 18s amplification is fine - (reply: 1)
783. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
784. What can I do to increase the banding signal? - (reply: 6)
785. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
786. Tomato Actin PCR+Images included - (reply: 2)
787. single primer PCR - (reply: 5)
788. Weird qPCR curves - (reply: 4)
789. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
790. LacZ PCR Problems - (reply: 1)
791. Primer concentration - (reply: 4)
792. nested PCR with low target DNA? - (reply: 2)
793. RT-PCR calibrator - (reply: 2)
794. problem in pcr - (reply: 2)
795. Can someone recommend me a virtual PCR software? - (reply: 6)
796. negative values after GOI - reference gene ?? - (reply: 4)
797. RNA degradation during electrophoresis?? - (reply: 2)
798. Primer 3' mismatch - Strange experience (reply: 9)
799. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
800. Adding loading/tracking dye to PCR mix? - (reply: 10)
801. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
802. internal control in RT-PCR - inquiry (reply: 2)
803. Melting Temp of Standards Changed? - (reply: 3)
804. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
805. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
806. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
807. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
808. Gene on negative strand - two eductional questions. - (reply: 3)
809. Newbie- choose DNA template - (reply: 1)
810. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)