Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
781. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
782. qPCR amplification interference fixed with freezing - (reply: 6)
783. Suggest me a mastermix for conventinal PCR... - (reply: 11)
784. reaction efficiency - (reply: 4)
785. software for quantifing PCR product band - (reply: 1)
786. Thermal cycler - (reply: 2)
787. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
788. qPCR volume - Problem with volume difference and results (reply: 1)
789. RT-PCR - (reply: 9)
790. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
791. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
792. cDNA synthesis - (reply: 6)
793. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
794. Primer seq - (reply: 2)
795. Selection of housekeeping gene in Mycobacterium - (reply: 1)
796. Do I need to pool the base line samples together - (reply: 2)
797. PCR product size - (reply: 6)
798. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
799. Few basic questions about RNA - (reply: 3)
800. oligo dt and random primer - (reply: 3)
801. Ct's of goi higher and lower than ct's hkg - (reply: 1)
802. Effect of collagenase on qpcr results - (reply: 1)
803. RNA Isolation - (reply: 3)
804. mRNA Search for RT-PCR (U to T) - (reply: 2)
805. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
806. PCR triplicates versus one reaction - differences? (reply: 17)
807. cloning of race products - (reply: 1)
808. Single or double-stranded cDNA? - (reply: 2)
809. Comparing SYBR and probe-based Ct values - Is it valid? (reply: 2)
810. qPCR inhibition - (reply: 1)