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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
781. PCR, neg control & no. of cycles - (reply: 2)
782. How do I insert a DNA sequence into a multiple cloning site and add restriction - (reply: 1)
783. efficiency calculation from non-10 fold dilutions - (reply: 1)
784. whole plasmid pcr? - (reply: 6)
785. Proper storage of isolated white blood cell prior to RNA isolation - (reply: 3)
786. Genomic pseudogenes?!? - (reply: 6)
787. Strange amplification plot - (reply: 4)
788. Conventional PCR of different dilutions - (reply: 1)
789. primer resuspension - (reply: 2)
790. Principles of overlap PCR - (reply: 7)
791. Help designing primers with restriction sites - (reply: 3)
792. DNA sequence data came back with lots of wrong bases - (reply: 5)
793. Why primers shouldn't have Tm difference greater than 5C? - (reply: 5)
794. Abnormal amplification curve - (reply: 1)
795. Problem with Semiquantitative PCR - (reply: 4)
796. false positive/cross reaction with TaqMan - (reply: 1)
797. Problem w/ Genotyping Cre Only - (reply: 3)
798. ROX reference dye for quantitative RT-PCR - (reply: 1)
799. PCR problem - (reply: 9)
800. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
801. why PCR product smeared? - (reply: 2)
802. Tm calculation for primers with RE sites and overhangs - (reply: 4)
803. silincing box, no amplification - (reply: 3)
804. Comparative semiquantitative RT-PCR - (reply: 2)
805. Bands in my PCR Controls - (reply: 3)
806. RTPCR - housekeeping genes: can I use them more intelligently? - (reply: 4)
807. real time qRT-PCR and low expression gene - (reply: 2)
808. In a pcr why doesnt the DNA reneal during annealing process ? - (reply: 2)
809. check points while preparing for sequencing - (reply: 10)
810. Disagreement between triplicate results in Q -PCR - (reply: 5)