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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
781. Error bars for qPCR; technical vs. biological replicate - (reply: 2)
782. HaCaT reference gene - (reply: 1)
783. Need help for PCR for AT rich gene - (reply: 3)
784. Amplify fragment in plasmid? - (reply: 4)
785. Relative quantification of different genes in single sample - same threshold val - (reply: 1)
786. Primer validation and PCR efficiency - (reply: 4)
787. How to calculate the price of PCR test per sample? - (reply: 6)
788. dilution help - (reply: 6)
789. rityProbe Integrity in Mastermix during loading 96 well plate - (reply: 2)
790. cloning with nested pcr - (reply: 3)
791. how to find 16srRNA sequences? - (reply: 3)
792. RNA extraction from a 1mm brain tissue punch - uncertain about homogenisation - (reply: 2)
793. What is the maxium amount of glycerol can be added in a PCR reaction? - (reply: 3)
794. PCR is amplifying non-specific fragment - (reply: 6)
795. Anybody know about temperature sensitivity of the AB TaqMan universal Mastermix - (reply: 4)
796. Measuring of cDNA bands density - (reply: 3)
797. real time RT-PCR problems - (reply: 2)
798. Maximum Annealing-Temperature for Real-Time? - (reply: 3)
799. Real-Time efficiencies and the pfaffl method - (reply: 1)
800. should I design primers spanning both intron and exon - (reply: 2)
801. RT-PCR - (reply: 1)
802. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
803. PCR with a very long and a short primer - (reply: 5)
804. qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
805. How to find that my RNA is not contaminated with gDNA, before running Real-Time - (reply: 5)
806. Dilution of 18s. How much? - (reply: 1)
807. Multiplex PCR does not work - (reply: 3)
808. Alternative to Sybr-green? - (reply: 12)
809. Multiple internal controls in using qPCR to measure gene duplication - (reply: 1)
810. ct value of neg - (reply: 1)