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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
571. real time PCR analysis in patient samples - (reply: 2)
572. Experiment plan for RT-qPCR internal reference search - (reply: 3)
573. Primer Check? - (reply: 2)
574. amplification of 14Kb - (reply: 1)
575. Validation of PCR primers/ probes - (reply: 6)
576. genomic DNA extraction from formalin fixed tissues (not embedded) for genotyping - (reply: 4)
577. Real time PCR sudenly not working - (reply: 1)
578. Buffer-composition One-Step RT-PCR - (reply: 4)
579. Two reverse primer sequences for a single forward primer - (reply: 1)
580. Tm variation - (reply: 1)
581. PCR with more than one primer - (reply: 1)
582. RAPD - PCR problem - (reply: 5)
583. Relative fold expression and Normalization fold expression - (reply: 2)
584. Strange Bands - (reply: 2)
585. Recommended tamplate DNA concentration for qPCR - (reply: 2)
586. RT / cDNA synthesis - (reply: 7)
587. How to best store PCR product? - (reply: 2)
588. smearing below band of interest - (reply: 5)
589. RT-PCR primer design - (reply: 1)
590. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
591. Odd gel run following PCR - (reply: 28)
592. Qiagen PCR Array Reagents? - (reply: 1)
593. Enough checks for gDNA contamination in qPCR? - (reply: 3)
594. plate stuck in ABI7300 - (reply: 7)
595. No or very weak band using 16s primers - (reply: 1)
596. No PCR amplification with b-actin primers - (reply: 1)
597. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
598. optical qPCR plates - (reply: 1)
599. Fold change significance - (reply: 2)
600. No PCR amplified with long primers - (reply: 10)