Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
571. Shift in peaks at dissociation curve - (reply: 4)
572. High resolution melting analysis-SNP genotyping. - (reply: 1)
573. PCR - (reply: 2)
574. nonspecific band at 50bp in PCR - (reply: 9)
575. cDNA sequencing - (reply: 2)
576. Maximum PCR product Tm - (reply: 6)
577. QuikChange with Different plasmids - (reply: 1)
578. Good melting curve but non-specific products appeared - (reply: 2)
579. Long Non-Specific PCR Products - (reply: 5)
580. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
581. 5'RACE of RNA Pol3 transcripts? - (reply: 3)
582. fold change (how to represent it) - (reply: 1)
583. PCR - consistent false positive results - (reply: 6)
584. geNorm alternative for qPCR housekeeping genes - (reply: 1)
585. PCR no amplification - (reply: 15)
586. positive NTC in real time reaction - (reply: 12)
587. Upscaling cDNA synthesis using SuperScript™ III Reverse Transcriptase - (reply: 4)
588. How to quantify virus by PCR - (reply: 12)
589. qPCR contamination problem - (reply: 5)
590. Error rate of commecial reverse transcriptase - (reply: 1)
591. Why do i get DNA of low concentrate after purification with kit - (reply: 9)
592. low A260/230 problem - (reply: 2)
593. What is a use of ΔCt and ΔΔCt in qRT PCR? - (reply: 4)
594. Pcr product size determination for a fusion gene - (reply: 8)
595. open ABI 7500 documents in Macbook - (reply: 1)
596. Primer Annealing Temperatures - (reply: 24)
597. Molecular beacons problem - (reply: 6)
598. RT-qPCR plate set up NoRT controls - (reply: 1)
599. improving sensitivity of one step TAQMAN REal time PCR - (reply: 1)
600. same primers, different product in conventional PCR and qPCR - (reply: 6)