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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
571. cDNA sequencing - (reply: 2)
572. Maximum PCR product Tm - (reply: 6)
573. QuikChange with Different plasmids - (reply: 1)
574. Good melting curve but non-specific products appeared - (reply: 2)
575. Long Non-Specific PCR Products - (reply: 5)
576. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
577. 5'RACE of RNA Pol3 transcripts? - (reply: 3)
578. fold change (how to represent it) - (reply: 1)
579. PCR - consistent false positive results - (reply: 6)
580. geNorm alternative for qPCR housekeeping genes - (reply: 1)
581. PCR no amplification - (reply: 15)
582. positive NTC in real time reaction - (reply: 12)
583. Upscaling cDNA synthesis using SuperScript™ III Reverse Transcriptase - (reply: 4)
584. How to quantify virus by PCR - (reply: 12)
585. qPCR contamination problem - (reply: 5)
586. Error rate of commecial reverse transcriptase - (reply: 1)
587. Why do i get DNA of low concentrate after purification with kit - (reply: 9)
588. low A260/230 problem - (reply: 2)
589. What is a use of ΔCt and ΔΔCt in qRT PCR? - (reply: 4)
590. Pcr product size determination for a fusion gene - (reply: 8)
591. open ABI 7500 documents in Macbook - (reply: 1)
592. Primer Annealing Temperatures - (reply: 24)
593. Molecular beacons problem - (reply: 6)
594. RT-qPCR plate set up NoRT controls - (reply: 1)
595. improving sensitivity of one step TAQMAN REal time PCR - (reply: 1)
596. same primers, different product in conventional PCR and qPCR - (reply: 6)
597. NEB Q5 polymerase works very well - (reply: 1)
598. Plateau height qPCR - (reply: 3)
599. UDG in qPCR - (reply: 1)
600. 5'RACE - (reply: 3)