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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
571. acceptable variation for biological replicate - (reply: 2)
572. RT-PCR contamination in negative control. - (reply: 1)
573. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
574. Technical problem with ABI7300 - (reply: 4)
575. cdna synthesis - (reply: 1)
576. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
577. Help with primer design - (reply: 3)
578. SPUD Assay Standard Curve "bell-shaped" - (reply: 5)
579. PCR smearing and...problems! - (reply: 7)
580. Message Amount in qPCR - (reply: 1)
581. Running Primers on Agarose Gel - (reply: 6)
582. Explanation needed for melting curve - (reply: 3)
583. cDNA concentration of qPCR - (reply: 1)
584. pGEX vector - (reply: 2)
585. will the template of PCR be added A ? - (reply: 2)
586. qPCR normalization of reference gene - (reply: 1)
587. Primer design: Tm above or below Ta? - (reply: 5)
588. Design primers for expression cloning - (reply: 11)
589. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
590. primer dilution query - (reply: 2)
591. Real Time PCR polymerase enzyme - (reply: 3)
592. housekeeping gene has not uniform pattern - (reply: 3)
593. Using BLAST to check primers - (reply: 1)
594. What is the function of a third primer in a PCR mix?? - (reply: 2)
595. How can we design a primer ? step by step method - (reply: 4)
596. Desperate! qPCR standard curve problems - (reply: 2)
597. cDNA synthesis or RT-PCR - (reply: 3)
598. PCR on cDNA and SGamplification??? - (reply: 1)
599. real time PCR protocol - controls? - (reply: 2)
600. Long primers (60bp) and 5kb PCR product problems - (reply: 5)