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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
571. qPCR normalization of reference gene - (reply: 1)
572. Primer design: Tm above or below Ta? - (reply: 5)
573. Design primers for expression cloning - (reply: 11)
574. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
575. primer dilution query - (reply: 2)
576. Real Time PCR polymerase enzyme - (reply: 3)
577. housekeeping gene has not uniform pattern - (reply: 3)
578. Using BLAST to check primers - (reply: 1)
579. What is the function of a third primer in a PCR mix?? - (reply: 2)
580. How can we design a primer ? step by step method - (reply: 4)
581. Desperate! qPCR standard curve problems - (reply: 2)
582. cDNA synthesis or RT-PCR - (reply: 3)
583. PCR on cDNA and SGamplification??? - (reply: 1)
584. real time PCR protocol - controls? - (reply: 2)
585. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
586. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
587. SOS: new real time PCR machine needed.... which one? - (reply: 5)
588. Amplicon side products - (reply: 5)
589. Fold change graph - (reply: 1)
590. Statistical analyses of 2 - delta delta Ct values - (reply: 2)
591. Finding the right sequence - (reply: 4)
592. Buy BAC vectors - (reply: 1)
593. meaningless real time results - (reply: 1)
594. RT-PCR in a single tube - (reply: 3)
595. why Phusion polymerase is not recommended for overlap PCR? - (reply: 1)
596. annealing temperature for 3 primer pairs? - (reply: 4)
597. Heated-lid - (reply: 3)
598. DEPC treated water Vs HPLC grade water - (reply: 2)
599. PCR Reaction - (reply: 3)
600. How to interpret this agarose gel? - (reply: 6)