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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
571. Primer Efficiency - (reply: 2)
572. data analysis for RT_PCR - (reply: 4)
573. Experimental set up for qRT PCR - (reply: 13)
574. What temp the lid of the thermocycler must be set? - (reply: 1)
575. How should I optimize my PCR - (reply: 2)
576. Real time data analysis using 2 machines - (reply: 3)
577. Second round of PCR after gel extraction fails miserably - (reply: 3)
578. urgent help plz with RT-PCR - (reply: 3)
579. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
580. LightCycler 480 Melting Curve Assay - (reply: 1)
581. Problems with two step RT-PCR - (reply: 2)
582. Spliced and unspliced form of a gene - (reply: 3)
583. primer dilutions - (reply: 4)
584. Genotyping PCR primers - (reply: 1)
585. Standard curve using plasmid - (reply: 1)
586. How to compare these results? Please comment. - (reply: 5)
587. Primers too dilute - (reply: 1)
588. Amplification curves jagged - (reply: 1)
589. ddCT issue: no endogenous expression in control. - (reply: 4)
590. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
591. No PCR product in site-directed mutagenesis - (reply: 4)
592. Giardia/Crypto real time PCR late amplification - (reply: 1)
593. How to dilute the template DNA for PCR - (reply: 7)
594. Desired length of overlap region for OE-PCR - (reply: 6)
595. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
596. Need help for PCR - (reply: 5)
597. scorpion probe - (reply: 8)
598. Too low Ct value for reference gene !!! - (reply: 3)
599. unespecific band PCR from mouse genomic DNA - (reply: 9)
600. Help with primer design through PRIMER3 - (reply: 4)