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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
571. Amplification curves jagged - (reply: 1)
572. ddCT issue: no endogenous expression in control. - (reply: 4)
573. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
574. No PCR product in site-directed mutagenesis - (reply: 4)
575. Giardia/Crypto real time PCR late amplification - (reply: 1)
576. How to dilute the template DNA for PCR - (reply: 7)
577. Desired length of overlap region for OE-PCR - (reply: 6)
578. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
579. Need help for PCR - (reply: 5)
580. scorpion probe - (reply: 8)
581. Too low Ct value for reference gene !!! - (reply: 3)
582. unespecific band PCR from mouse genomic DNA - (reply: 9)
583. Help with primer design through PRIMER3 - (reply: 4)
584. Help with unknown band in restriction digest - (reply: 1)
585. how to chose right subsequent for her2(erbb2)gene ? - (reply: 3)
586. 454 sequencing - (reply: 2)
587. Preference for larger of 2 bands? - (reply: 2)
588. Tm of primers - prediction and verification - (reply: 1)
589. acceptable variation for biological replicate - (reply: 2)
590. RT-PCR contamination in negative control. - (reply: 1)
591. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
592. Technical problem with ABI7300 - (reply: 4)
593. cdna synthesis - (reply: 1)
594. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
595. Help with primer design - (reply: 3)
596. SPUD Assay Standard Curve "bell-shaped" - (reply: 5)
597. PCR smearing and...problems! - (reply: 7)
598. Message Amount in qPCR - (reply: 1)
599. Running Primers on Agarose Gel - (reply: 6)
600. Explanation needed for melting curve - (reply: 3)