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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
121. website for primer design - (reply: 1)
122. PCR Efficiency over 150%! - (reply: 1)
123. problem of amplification - (reply: 2)
124. Rxn not working - amplify/linearize plasmid - (reply: 3)
125. qRT-PCR works one day, and not the next - (reply: 1)
126. PCR not working - (reply: 11)
127. an urgent problem with replicates - (reply: 10)
128. Site-directed mutagenesis: maximum substitution - (reply: 1)
129. Inter run calibration qRT-PCR - (reply: 2)
130. Primer design and alternative transcripts - (reply: 2)
131. qPCR amplification - (reply: 4)
132. Extremely desperate noob question: How do these PCR work? - (reply: 6)
133. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
134. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)
135. RT-PCR - High Ct Values and Laser Capture - (reply: 1)
136. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
137. Genomic DNA Isolation from Soft Agar Colonies - (reply: 3)
138. help with total RNA isolation - (reply: 1)
139. Need help to identify bands in AGE result - (reply: 3)
140. COLD-PCR (Dissociation Curve) via qPCR - (reply: 4)
141. how to hasten real-time PCR amplifications - (reply: 2)
142. Please please help me with my Phusion PCR. - (reply: 5)
143. Nanodrop vs Picogreen to quantify post-PCR product - (reply: 3)
144. Need no RT control for all genes? - (reply: 1)
145. Different annealing temp between HKG and target gene - (reply: 2)
146. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
147. help in long pcr - (reply: 1)
148. Reselling a supermix/enzyme in a commercial diagnostic kit - (reply: 2)
149. Small yield of RNA after on column DNAs digestion , Why - (reply: 3)
150. PCR inhibitor in template DNA - (reply: 3)