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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
121. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
122. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
123. PCR gene specific amplification problem - (reply: 3)
124. Failure SYBRGREEN PCR - (reply: 4)
125. Pfaffl Method for relative - (reply: 4)
126. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
127. experiences with NuPCR from Illumina? - (reply: 1)
128. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
129. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
130. 100 ng RNA for cDNA synthesis - (reply: 4)
131. Inexplicable qPCR failure in single wells - (reply: 7)
132. negative control well 300 bp band - (reply: 3)
133. Reference gene for normalisation - for different growth rates - (reply: 3)
134. Strange amplification plots with high Ct variability - (reply: 1)
135. How big a role does mixing play in PCR - (reply: 1)
136. Melting curve is irregular for primer optimization - (reply: 5)
137. Designing primers for ABO blood groups - (reply: 1)
138. Methylight Results Analysis - (reply: 1)
139. GAPDH Ct value - (reply: 4)
140. dissociation curves - (reply: 1)
141. RT-PCR primer design - (reply: 7)
142. How to amplify very short PCR template - (reply: 4)
143. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
144. Is this primer okay? - (reply: 4)
145. Dilution calculation - (reply: 3)
146. Common causes for low RNA A260/230 ratios - (reply: 7)
147. Limit of detection for salmon gDNA? - (reply: 7)
148. Intensifying signal from positive control - (reply: 5)
149. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
150. analysing qpcr data and plotting standard curve - (reply: 1)