Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
121. Amplification in HK gene but not for target gene - (reply: 3)
122. Missing DNA Target and Effect on Non-Specific Amplicons - (reply: 1)
123. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
124. how to make this reaction? - (reply: 1)
125. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
126. How long can I store at - 30C my PCR mix? - (reply: 6)
127. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
128. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
129. RT-PCR help - (reply: 2)
130. Strange signals in the NTC - (reply: 1)
131. False positive - help - (reply: 3)
132. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
133. PCR Temperature change control malfunctioning - (reply: 1)
134. Assay question - (reply: 1)
135. WHY NO ONE REPLIES, is this a stupid question ? - (reply: 5)
136. HRM instrumentaion - (reply: 4)
137. Can you repeat a thermocycle? - (reply: 3)
138. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
139. Amplification curve in the negative control samples - (reply: 7)
140. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
141. Primer as limiting reagent in PCR reaction - (reply: 2)
142. Taqman probe in a LightCycler 480? - (reply: 1)
143. Real time PCR for degraded RNA - (reply: 1)
144. Ct value of housekeeping gene - (reply: 4)
145. Efficiency correct fold change -- use all or none? - (reply: 1)
146. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
147. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
148. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
149. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
150. Is this primer-dimer peak in my melting curve? - (reply: 6)