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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
511. SYBR vs FAM probe - (reply: 1)
512. Quantify gDNA in buccal swab - (reply: 2)
513. qPCR: band in gel, but super high Cq? - (reply: 10)
514. Melt curve analysis - (reply: 5)
515. Colony PCR - M13 vs. gene-specific primer amplification of bacmid - (reply: 4)
516. How to Compare CT values between treated and untreated bacteria with uneven grow - (reply: 1)
517. DNA amplification differences between samples & between regions (WGA & s - (reply: 2)
518. wrong dntp concentration - (reply: 2)
519. Which one is better as reference gene base on these data - (reply: 3)
520. About the qPCRs and statistics - (reply: 2)
521. long fragment PCR - (reply: 2)
522. Replacing one reagent by another in a protocol (Please help) - (reply: 5)
523. RT-qPCR normalization - (reply: 3)
524. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
525. I need to break this cycle of PCR issues - (reply: 13)
526. Questions / Help with Fusion PCR - (reply: 8)
527. Controls for RT-PCR reactions - (reply: 2)
528. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
529. qPCR with several BIOLOGICAL REPLICATES, how to compare? - (reply: 1)
530. issues when designing PCR primers using PRIMER3 - (reply: 8)
531. Primer catalog/databasing - (reply: 1)
532. Nonrepetitive nested PCR - (reply: 2)
533. DNA or cDNA? - (reply: 1)
534. Difference in Ct value of replicates - (reply: 1)
535. TDNA Genotyping PCR program - (reply: 1)
536. Shift in peaks at dissociation curve - (reply: 4)
537. High resolution melting analysis-SNP genotyping. - (reply: 1)
538. PCR - (reply: 2)
539. nonspecific band at 50bp in PCR - (reply: 9)
540. cDNA sequencing - (reply: 2)