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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
511. Primer Efficiency - (reply: 2)
512. data analysis for RT_PCR - (reply: 4)
513. Experimental set up for qRT PCR - (reply: 13)
514. What temp the lid of the thermocycler must be set? - (reply: 1)
515. How should I optimize my PCR - (reply: 2)
516. Real time data analysis using 2 machines - (reply: 3)
517. Second round of PCR after gel extraction fails miserably - (reply: 3)
518. urgent help plz with RT-PCR - (reply: 3)
519. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
520. LightCycler 480 Melting Curve Assay - (reply: 1)
521. Problems with two step RT-PCR - (reply: 2)
522. Spliced and unspliced form of a gene - (reply: 3)
523. primer dilutions - (reply: 4)
524. Genotyping PCR primers - (reply: 1)
525. Standard curve using plasmid - (reply: 1)
526. How to compare these results? Please comment. - (reply: 5)
527. Primers too dilute - (reply: 1)
528. Amplification curves jagged - (reply: 1)
529. ddCT issue: no endogenous expression in control. - (reply: 4)
530. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
531. No PCR product in site-directed mutagenesis - (reply: 4)
532. Giardia/Crypto real time PCR late amplification - (reply: 1)
533. How to dilute the template DNA for PCR - (reply: 7)
534. Desired length of overlap region for OE-PCR - (reply: 6)
535. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
536. Need help for PCR - (reply: 5)
537. scorpion probe - (reply: 8)
538. Too low Ct value for reference gene !!! - (reply: 3)
539. unespecific band PCR from mouse genomic DNA - (reply: 9)
540. Help with primer design through PRIMER3 - (reply: 4)