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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
511. How should I optimize my PCR - (reply: 2)
512. Real time data analysis using 2 machines - (reply: 3)
513. Second round of PCR after gel extraction fails miserably - (reply: 3)
514. urgent help plz with RT-PCR - (reply: 3)
515. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
516. LightCycler 480 Melting Curve Assay - (reply: 1)
517. Problems with two step RT-PCR - (reply: 2)
518. Spliced and unspliced form of a gene - (reply: 3)
519. primer dilutions - (reply: 4)
520. Genotyping PCR primers - (reply: 1)
521. Standard curve using plasmid - (reply: 1)
522. How to compare these results? Please comment. - (reply: 5)
523. Primers too dilute - (reply: 1)
524. Amplification curves jagged - (reply: 1)
525. ddCT issue: no endogenous expression in control. - (reply: 4)
526. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
527. No PCR product in site-directed mutagenesis - (reply: 4)
528. Giardia/Crypto real time PCR late amplification - (reply: 1)
529. How to dilute the template DNA for PCR - (reply: 7)
530. Desired length of overlap region for OE-PCR - (reply: 6)
531. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
532. Need help for PCR - (reply: 5)
533. scorpion probe - (reply: 8)
534. Too low Ct value for reference gene !!! - (reply: 3)
535. unespecific band PCR from mouse genomic DNA - (reply: 9)
536. Help with primer design through PRIMER3 - (reply: 4)
537. Help with unknown band in restriction digest - (reply: 1)
538. how to chose right subsequent for her2(erbb2)gene ? - (reply: 3)
539. 454 sequencing - (reply: 2)
540. Preference for larger of 2 bands? - (reply: 2)