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PCR--RT-PCR-and-Real-Time-PCR
511.
add template first or Syber mix first to reduce contamination in qPCR? -
(reply: 3)
512.
Strange looking "elevated" amplification plots from ChIP-qPCR samples -
(reply: 2)
513.
PCR-screening of T-DNA inserted in Arabidopsis gene -
(reply: 1)
514.
iCycler Calibration Question -
(reply: 1)
515.
Long PCR (>10kb) polymerase recommendations -
(reply: 3)
516.
thermocycler - real-time versus conventional? -
(reply: 2)
517.
I need help for designing a pair of primers for this sequence. -
(reply: 1)
518.
Change in Superscript lot influences transcript level of one gene but not others -
(reply: 2)
519.
1/delta Ct vs delta delat CT -
(reply: 1)
520.
PCR band slightly BELOW expected length ?! -
(reply: 14)
521.
Does anyone have any problem with Quanta Sybr Green -
(reply: 2)
522.
Trouble correlating GU/ml qPCR results to cfu/ml plating results -
(reply: 2)
523.
long primers PCR -
(reply: 4)
524.
unexpected band in PCR with plasmid -
(reply: 4)
525.
qPCR [template] and [primer] troubleshooting...... -
(reply: 1)
526.
Good Protocol for Semi-Quantitative RT-PCR? -
(reply: 1)
527.
DNA extraction - PCR Problem -
(reply: 3)
528.
Real Time Trouble, low cTs -
(reply: 3)
529.
interpretation of results -
(reply: 8)
530.
Multiple Bands in qPCR -
(reply: 4)
531.
PCR product biotinylation -
(reply: 3)
532.
Tagged primers and a second PCR targetting the tags -
(reply: 5)
533.
Question about isolating DNA from the blood sample -
(reply: 2)
534.
RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR -
(reply: 3)
535.
Universal ProbeLibrary for Human by ROCHE -
(reply: 1)
536.
role of water in PCR -
(reply: 3)
537.
minimum amount of RNA for RT_PCR -
(reply: 1)
538.
Is it possible to compare abundance of isoforms by qPCR -
(reply: 6)
539.
HIV gene- PCR -
(reply: 3)
540.
Real time PCR melting curve -
(reply: 2)
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