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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
511. ddCT issue: no endogenous expression in control. - (reply: 4)
512. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
513. No PCR product in site-directed mutagenesis - (reply: 4)
514. Giardia/Crypto real time PCR late amplification - (reply: 1)
515. How to dilute the template DNA for PCR - (reply: 7)
516. Desired length of overlap region for OE-PCR - (reply: 6)
517. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
518. Need help for PCR - (reply: 5)
519. scorpion probe - (reply: 8)
520. Too low Ct value for reference gene !!! - (reply: 3)
521. unespecific band PCR from mouse genomic DNA - (reply: 9)
522. Help with primer design through PRIMER3 - (reply: 4)
523. Help with unknown band in restriction digest - (reply: 1)
524. how to chose right subsequent for her2(erbb2)gene ? - (reply: 3)
525. 454 sequencing - (reply: 2)
526. Preference for larger of 2 bands? - (reply: 2)
527. Tm of primers - prediction and verification - (reply: 1)
528. acceptable variation for biological replicate - (reply: 2)
529. RT-PCR contamination in negative control. - (reply: 1)
530. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
531. Technical problem with ABI7300 - (reply: 4)
532. cdna synthesis - (reply: 1)
533. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
534. Help with primer design - (reply: 3)
535. SPUD Assay Standard Curve "bell-shaped" - (reply: 5)
536. PCR smearing and...problems! - (reply: 7)
537. Message Amount in qPCR - (reply: 1)
538. Running Primers on Agarose Gel - (reply: 6)
539. Explanation needed for melting curve - (reply: 3)
540. cDNA concentration of qPCR - (reply: 1)