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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
511. Melt curve analysis - (reply: 5)
512. Colony PCR - M13 vs. gene-specific primer amplification of bacmid - (reply: 4)
513. How to Compare CT values between treated and untreated bacteria with uneven grow - (reply: 1)
514. DNA amplification differences between samples & between regions (WGA & s - (reply: 2)
515. wrong dntp concentration - (reply: 2)
516. Which one is better as reference gene base on these data - (reply: 3)
517. About the qPCRs and statistics - (reply: 2)
518. long fragment PCR - (reply: 2)
519. Replacing one reagent by another in a protocol (Please help) - (reply: 5)
520. RT-qPCR normalization - (reply: 3)
521. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
522. I need to break this cycle of PCR issues - (reply: 13)
523. Questions / Help with Fusion PCR - (reply: 8)
524. Controls for RT-PCR reactions - (reply: 2)
525. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
526. qPCR with several BIOLOGICAL REPLICATES, how to compare? - (reply: 1)
527. issues when designing PCR primers using PRIMER3 - (reply: 8)
528. Primer catalog/databasing - (reply: 1)
529. Nonrepetitive nested PCR - (reply: 2)
530. DNA or cDNA? - (reply: 1)
531. Difference in Ct value of replicates - (reply: 1)
532. TDNA Genotyping PCR program - (reply: 1)
533. Shift in peaks at dissociation curve - (reply: 4)
534. High resolution melting analysis-SNP genotyping. - (reply: 1)
535. PCR - (reply: 2)
536. nonspecific band at 50bp in PCR - (reply: 9)
537. cDNA sequencing - (reply: 2)
538. Maximum PCR product Tm - (reply: 6)
539. QuikChange with Different plasmids - (reply: 1)
540. Good melting curve but non-specific products appeared - (reply: 2)