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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
511. Transcription of PCR products without a primer coupled with promotor sequence - (reply: 4)
512. Normalization to Standard Gene: what does this mean - (reply: 8)
513. isolating DNA from mouse tails and subsequent problems with pcr - (reply: 7)
514. How to Join two PCR products - (reply: 3)
515. Reference RNA for qPCR - (reply: 3)
516. Restriction enzyme - PCR sheep blood gDNA - (reply: 5)
517. What's wrong with my PCR - (reply: 5)
518. What does 'prevention of nontemplate-directed nucleotide addition' mean? - (reply: 3)
519. loss or results (ABI prism 7000) - (reply: 6)
520. Ct values - (reply: 3)
521. Problem with UPL real-time PCR - please help !!! - (reply: 1)
522. qRT-PCR data analysis - (reply: 2)
523. Cp, but no amplification - (reply: 2)
524. Strange RT to qPCR results with new kit - (reply: 5)
525. Low PCR product - (reply: 7)
526. SYBR vs FAM probe - (reply: 1)
527. Quantify gDNA in buccal swab - (reply: 2)
528. qPCR: band in gel, but super high Cq? - (reply: 10)
529. Melt curve analysis - (reply: 5)
530. Colony PCR - M13 vs. gene-specific primer amplification of bacmid - (reply: 4)
531. How to Compare CT values between treated and untreated bacteria with uneven grow - (reply: 1)
532. DNA amplification differences between samples & between regions (WGA & s - (reply: 2)
533. wrong dntp concentration - (reply: 2)
534. Which one is better as reference gene base on these data - (reply: 3)
535. About the qPCRs and statistics - (reply: 2)
536. long fragment PCR - (reply: 2)
537. Replacing one reagent by another in a protocol (Please help) - (reply: 5)
538. RT-qPCR normalization - (reply: 3)
539. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
540. I need to break this cycle of PCR issues - (reply: 13)