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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
511. Real time data analysis using 2 machines - (reply: 3)
512. Second round of PCR after gel extraction fails miserably - (reply: 3)
513. urgent help plz with RT-PCR - (reply: 3)
514. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
515. LightCycler 480 Melting Curve Assay - (reply: 1)
516. Problems with two step RT-PCR - (reply: 2)
517. Spliced and unspliced form of a gene - (reply: 3)
518. primer dilutions - (reply: 4)
519. Genotyping PCR primers - (reply: 1)
520. Standard curve using plasmid - (reply: 1)
521. How to compare these results? Please comment. - (reply: 5)
522. Primers too dilute - (reply: 1)
523. Amplification curves jagged - (reply: 1)
524. ddCT issue: no endogenous expression in control. - (reply: 4)
525. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
526. No PCR product in site-directed mutagenesis - (reply: 4)
527. Giardia/Crypto real time PCR late amplification - (reply: 1)
528. How to dilute the template DNA for PCR - (reply: 7)
529. Desired length of overlap region for OE-PCR - (reply: 6)
530. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
531. Need help for PCR - (reply: 5)
532. scorpion probe - (reply: 8)
533. Too low Ct value for reference gene !!! - (reply: 3)
534. unespecific band PCR from mouse genomic DNA - (reply: 9)
535. Help with primer design through PRIMER3 - (reply: 4)
536. Help with unknown band in restriction digest - (reply: 1)
537. how to chose right subsequent for her2(erbb2)gene ? - (reply: 3)
538. 454 sequencing - (reply: 2)
539. Preference for larger of 2 bands? - (reply: 2)
540. Tm of primers - prediction and verification - (reply: 1)