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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
511. Cp, but no amplification - (reply: 2)
512. Strange RT to qPCR results with new kit - (reply: 5)
513. Low PCR product - (reply: 7)
514. SYBR vs FAM probe - (reply: 1)
515. Quantify gDNA in buccal swab - (reply: 2)
516. qPCR: band in gel, but super high Cq? - (reply: 10)
517. Melt curve analysis - (reply: 5)
518. Colony PCR - M13 vs. gene-specific primer amplification of bacmid - (reply: 4)
519. How to Compare CT values between treated and untreated bacteria with uneven grow - (reply: 1)
520. DNA amplification differences between samples & between regions (WGA & s - (reply: 2)
521. wrong dntp concentration - (reply: 2)
522. Which one is better as reference gene base on these data - (reply: 3)
523. About the qPCRs and statistics - (reply: 2)
524. long fragment PCR - (reply: 2)
525. Replacing one reagent by another in a protocol (Please help) - (reply: 5)
526. RT-qPCR normalization - (reply: 3)
527. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
528. I need to break this cycle of PCR issues - (reply: 13)
529. Questions / Help with Fusion PCR - (reply: 8)
530. Controls for RT-PCR reactions - (reply: 2)
531. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
532. qPCR with several BIOLOGICAL REPLICATES, how to compare? - (reply: 1)
533. issues when designing PCR primers using PRIMER3 - (reply: 8)
534. Primer catalog/databasing - (reply: 1)
535. Nonrepetitive nested PCR - (reply: 2)
536. DNA or cDNA? - (reply: 1)
537. Difference in Ct value of replicates - (reply: 1)
538. TDNA Genotyping PCR program - (reply: 1)
539. Shift in peaks at dissociation curve - (reply: 4)
540. High resolution melting analysis-SNP genotyping. - (reply: 1)