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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
511. Questions / Help with Fusion PCR - (reply: 8)
512. Controls for RT-PCR reactions - (reply: 2)
513. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
514. qPCR with several BIOLOGICAL REPLICATES, how to compare? - (reply: 1)
515. issues when designing PCR primers using PRIMER3 - (reply: 8)
516. Primer catalog/databasing - (reply: 1)
517. Nonrepetitive nested PCR - (reply: 2)
518. DNA or cDNA? - (reply: 1)
519. Difference in Ct value of replicates - (reply: 1)
520. TDNA Genotyping PCR program - (reply: 1)
521. Shift in peaks at dissociation curve - (reply: 4)
522. High resolution melting analysis-SNP genotyping. - (reply: 1)
523. PCR - (reply: 2)
524. nonspecific band at 50bp in PCR - (reply: 9)
525. cDNA sequencing - (reply: 2)
526. Maximum PCR product Tm - (reply: 6)
527. QuikChange with Different plasmids - (reply: 1)
528. Good melting curve but non-specific products appeared - (reply: 2)
529. Long Non-Specific PCR Products - (reply: 5)
530. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
531. 5'RACE of RNA Pol3 transcripts? - (reply: 3)
532. fold change (how to represent it) - (reply: 1)
533. PCR - consistent false positive results - (reply: 6)
534. geNorm alternative for qPCR housekeeping genes - (reply: 1)
535. PCR no amplification - (reply: 15)
536. positive NTC in real time reaction - (reply: 12)
537. Upscaling cDNA synthesis using SuperScript™ III Reverse Transcriptase - (reply: 4)
538. How to quantify virus by PCR - (reply: 12)
539. qPCR contamination problem - (reply: 5)
540. Error rate of commecial reverse transcriptase - (reply: 1)