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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
511. Colony PCR - M13 vs. gene-specific primer amplification of bacmid - (reply: 4)
512. How to Compare CT values between treated and untreated bacteria with uneven grow - (reply: 1)
513. DNA amplification differences between samples & between regions (WGA & s - (reply: 2)
514. wrong dntp concentration - (reply: 2)
515. Which one is better as reference gene base on these data - (reply: 3)
516. About the qPCRs and statistics - (reply: 2)
517. long fragment PCR - (reply: 2)
518. Replacing one reagent by another in a protocol (Please help) - (reply: 5)
519. RT-qPCR normalization - (reply: 3)
520. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
521. I need to break this cycle of PCR issues - (reply: 13)
522. Questions / Help with Fusion PCR - (reply: 8)
523. Controls for RT-PCR reactions - (reply: 2)
524. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
525. qPCR with several BIOLOGICAL REPLICATES, how to compare? - (reply: 1)
526. issues when designing PCR primers using PRIMER3 - (reply: 8)
527. Primer catalog/databasing - (reply: 1)
528. Nonrepetitive nested PCR - (reply: 2)
529. DNA or cDNA? - (reply: 1)
530. Difference in Ct value of replicates - (reply: 1)
531. TDNA Genotyping PCR program - (reply: 1)
532. Shift in peaks at dissociation curve - (reply: 4)
533. High resolution melting analysis-SNP genotyping. - (reply: 1)
534. PCR - (reply: 2)
535. nonspecific band at 50bp in PCR - (reply: 9)
536. cDNA sequencing - (reply: 2)
537. Maximum PCR product Tm - (reply: 6)
538. QuikChange with Different plasmids - (reply: 1)
539. Good melting curve but non-specific products appeared - (reply: 2)
540. Long Non-Specific PCR Products - (reply: 5)