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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
511. long fragment PCR - (reply: 2)
512. Replacing one reagent by another in a protocol (Please help) - (reply: 5)
513. RT-qPCR normalization - (reply: 3)
514. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
515. I need to break this cycle of PCR issues - (reply: 13)
516. Questions / Help with Fusion PCR - (reply: 8)
517. Controls for RT-PCR reactions - (reply: 2)
518. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
519. qPCR with several BIOLOGICAL REPLICATES, how to compare? - (reply: 1)
520. issues when designing PCR primers using PRIMER3 - (reply: 8)
521. Primer catalog/databasing - (reply: 1)
522. Nonrepetitive nested PCR - (reply: 2)
523. DNA or cDNA? - (reply: 1)
524. Difference in Ct value of replicates - (reply: 1)
525. TDNA Genotyping PCR program - (reply: 1)
526. Shift in peaks at dissociation curve - (reply: 4)
527. High resolution melting analysis-SNP genotyping. - (reply: 1)
528. PCR - (reply: 2)
529. nonspecific band at 50bp in PCR - (reply: 9)
530. cDNA sequencing - (reply: 2)
531. Maximum PCR product Tm - (reply: 6)
532. QuikChange with Different plasmids - (reply: 1)
533. Good melting curve but non-specific products appeared - (reply: 2)
534. Long Non-Specific PCR Products - (reply: 5)
535. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
536. 5'RACE of RNA Pol3 transcripts? - (reply: 3)
537. fold change (how to represent it) - (reply: 1)
538. PCR - consistent false positive results - (reply: 6)
539. geNorm alternative for qPCR housekeeping genes - (reply: 1)
540. PCR no amplification - (reply: 15)