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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1321. Checking genomic contamination - different ways (reply: 4)
1322. Probe or Primer info for bovine AQP-1 - (reply: 6)
1323. PCR with Platinum Taq - product yield issues - (reply: 3)
1324. real time PCR - (reply: 7)
1325. very high and inconsistent Cts for the house keeping gene - (reply: 4)
1326. Standard curves, are they necessary every time we run an experiment? - (reply: 6)
1327. Sample handling for RNA extraction - (reply: 1)
1328. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
1329. Normalising RNA concentrations - Help (reply: 4)
1330. How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)
1331. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
1332. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
1333. Having abnormal dissociation curve when performing qPCR - (reply: 3)
1334. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
1335. Please help me if you could - (reply: 5)
1336. Tm caculation for different PCRs?? - new, confused, and frustrated beginner (reply: 3)
1337. absolute quantification - (reply: 4)
1338. The Case of a Missing Band: PCR Issue - (reply: 4)
1339. Log 10 fold serial dilutions - (reply: 2)
1340. paranormal qpcr amplification activity - melting curves (reply: 3)
1341. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
1342. PCR template concentration - (reply: 6)
1343. PCR efficiency calculated by Linreg - (reply: 3)
1344. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
1345. Stability of genomic DNA in 1x PBS - (reply: 2)
1346. primer design by generunner DG - (reply: 1)
1347. Calculation of Tm of amplicon when using SYBR Green - (reply: 3)
1348. degenerate pcr - (reply: 1)
1349. DNA degradation - (reply: 1)
1350. DNase 1 treratment of RNA - (reply: 11)