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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1321. Secondary structure in real time - (reply: 1)
1322. primers with fluoresent dye PCR cycle question - (reply: 4)
1323. House keeping gene question? - Thanks for any help! (reply: 1)
1324. Questions about Tween 20 - (reply: 1)
1325. General qRT-PCR question - (reply: 3)
1326. qPCR stats problem - Vandesompele's, DDct or other methods?? (reply: 1)
1327. Taqman probe not easily synthesized - What sequence features make probe synthesis difficult? (reply: 3)
1328. Smears on PCR products - (reply: 4)
1329. Dumb Reverse Transcription PCR Question - (reply: 2)
1330. qPCR absolute/relative quantification method question - (reply: 4)
1331. PCR-amplicon too short - (reply: 1)
1332. Reverse transcription PCR - (reply: 1)
1333. PCR/RT-PCR beads - (reply: 2)
1334. High Amplification Efficiency in Std. Curve - (reply: 5)
1335. Run PCR amplifications in agarose gels - (reply: 3)
1336. Problems with validating primers and low expression genes - (reply: 3)
1337. Compare different qPCR runs - really need help (reply: 8)
1338. General real time questions. - (reply: 1)
1339. Analysis HELP!!!! - (reply: 1)
1340. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
1341. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
1342. Mutagenesis woes - (reply: 3)
1343. KpnI Restriction enzyme - how to detect the SNP (reply: 1)
1344. intron/exon spanning primers - (reply: 1)
1345. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
1346. PCR product for sequencing - sample storage?? (reply: 2)
1347. Absolute quantification using Real Time PCR - (reply: 2)
1348. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
1349. High Ct value - what to do next? - (reply: 4)
1350. Biorad Mini Opticon - can use any 0.2ml tubes or Biorad only? (reply: 1)