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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1321. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
1322. Stability of genomic DNA in 1x PBS - (reply: 2)
1323. primer design by generunner DG - (reply: 1)
1324. Calculation of Tm of amplicon when using SYBR Green - (reply: 3)
1325. degenerate pcr - (reply: 1)
1326. DNA degradation - (reply: 1)
1327. DNase 1 treratment of RNA - (reply: 11)
1328. Secondary structure in real time - (reply: 1)
1329. primers with fluoresent dye PCR cycle question - (reply: 4)
1330. House keeping gene question? - Thanks for any help! (reply: 1)
1331. Questions about Tween 20 - (reply: 1)
1332. General qRT-PCR question - (reply: 3)
1333. qPCR stats problem - Vandesompele's, DDct or other methods?? (reply: 1)
1334. Taqman probe not easily synthesized - What sequence features make probe synthesis difficult? (reply: 3)
1335. Smears on PCR products - (reply: 4)
1336. Dumb Reverse Transcription PCR Question - (reply: 2)
1337. qPCR absolute/relative quantification method question - (reply: 4)
1338. PCR-amplicon too short - (reply: 1)
1339. Reverse transcription PCR - (reply: 1)
1340. PCR/RT-PCR beads - (reply: 2)
1341. High Amplification Efficiency in Std. Curve - (reply: 5)
1342. Run PCR amplifications in agarose gels - (reply: 3)
1343. Problems with validating primers and low expression genes - (reply: 3)
1344. Compare different qPCR runs - really need help (reply: 8)
1345. General real time questions. - (reply: 1)
1346. Analysis HELP!!!! - (reply: 1)
1347. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
1348. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
1349. Mutagenesis woes - (reply: 3)
1350. KpnI Restriction enzyme - how to detect the SNP (reply: 1)