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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1321. primer design by generunner DG - (reply: 1)
1322. Calculation of Tm of amplicon when using SYBR Green - (reply: 3)
1323. degenerate pcr - (reply: 1)
1324. DNA degradation - (reply: 1)
1325. DNase 1 treratment of RNA - (reply: 11)
1326. Secondary structure in real time - (reply: 1)
1327. primers with fluoresent dye PCR cycle question - (reply: 4)
1328. House keeping gene question? - Thanks for any help! (reply: 1)
1329. Questions about Tween 20 - (reply: 1)
1330. General qRT-PCR question - (reply: 3)
1331. qPCR stats problem - Vandesompele's, DDct or other methods?? (reply: 1)
1332. Taqman probe not easily synthesized - What sequence features make probe synthesis difficult? (reply: 3)
1333. Smears on PCR products - (reply: 4)
1334. Dumb Reverse Transcription PCR Question - (reply: 2)
1335. qPCR absolute/relative quantification method question - (reply: 4)
1336. PCR-amplicon too short - (reply: 1)
1337. Reverse transcription PCR - (reply: 1)
1338. PCR/RT-PCR beads - (reply: 2)
1339. High Amplification Efficiency in Std. Curve - (reply: 5)
1340. Run PCR amplifications in agarose gels - (reply: 3)
1341. Problems with validating primers and low expression genes - (reply: 3)
1342. Compare different qPCR runs - really need help (reply: 8)
1343. General real time questions. - (reply: 1)
1344. Analysis HELP!!!! - (reply: 1)
1345. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
1346. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
1347. Mutagenesis woes - (reply: 3)
1348. KpnI Restriction enzyme - how to detect the SNP (reply: 1)
1349. intron/exon spanning primers - (reply: 1)
1350. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)