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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1321. Touchdown PCR issues - (reply: 3)
1322. QPCR/DDCT Method/ Statistics - (reply: 2)
1323. LINE1 OFR2 Primer Design - (reply: 1)
1324. rna isolation manually - (reply: 2)
1325. phosphorylation of primers using PNK - (reply: 1)
1326. RT-PCR contamination issue - (reply: 3)
1327. What's the longest overhanging primer seq. you have used? - (reply: 2)
1328. Primer Concentration to lower Ct - (reply: 1)
1329. Primer Concentration Help - (reply: 4)
1330. Can normal sequencing services sequence >1kb product? - sequencing (reply: 1)
1331. who got a manual of ABI7500 PCR instrument ? - how to operate this instument ? (reply: 4)
1332. Primer Degradation - What exactly happens? (reply: 1)
1333. Smearing on ategarose gel of real time pcr product??? - (reply: 1)
1334. PCR/DNA Extraction Problem - (reply: 5)
1335. Primers in 2 exons - (reply: 2)
1336. NTC appear in real time pcr - (reply: 6)
1337. RNA solutions - Calculating concentrations (reply: 9)
1338. PCR of AT-rich DNA - (reply: 1)
1339. NTP mix (with dUTP instead of dTTP) in sybr green mix - (reply: 1)
1340. TBE Buffer? - (reply: 4)
1341. Overlapping PCR - really need help ! - (reply: 5)
1342. gel documentation sysytem - (reply: 7)
1343. internal standard - (reply: 5)
1344. Checking genomic contamination - different ways (reply: 4)
1345. Probe or Primer info for bovine AQP-1 - (reply: 6)
1346. PCR with Platinum Taq - product yield issues - (reply: 3)
1347. real time PCR - (reply: 7)
1348. very high and inconsistent Cts for the house keeping gene - (reply: 4)
1349. Standard curves, are they necessary every time we run an experiment? - (reply: 6)
1350. Sample handling for RNA extraction - (reply: 1)