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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1321. Primers in 2 exons - (reply: 2)
1322. NTC appear in real time pcr - (reply: 6)
1323. RNA solutions - Calculating concentrations (reply: 9)
1324. PCR of AT-rich DNA - (reply: 1)
1325. NTP mix (with dUTP instead of dTTP) in sybr green mix - (reply: 1)
1326. TBE Buffer? - (reply: 4)
1327. Overlapping PCR - really need help ! - (reply: 5)
1328. gel documentation sysytem - (reply: 7)
1329. internal standard - (reply: 5)
1330. Checking genomic contamination - different ways (reply: 4)
1331. Probe or Primer info for bovine AQP-1 - (reply: 6)
1332. PCR with Platinum Taq - product yield issues - (reply: 3)
1333. real time PCR - (reply: 7)
1334. very high and inconsistent Cts for the house keeping gene - (reply: 4)
1335. Standard curves, are they necessary every time we run an experiment? - (reply: 6)
1336. Sample handling for RNA extraction - (reply: 1)
1337. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
1338. Normalising RNA concentrations - Help (reply: 4)
1339. How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)
1340. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
1341. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
1342. Having abnormal dissociation curve when performing qPCR - (reply: 3)
1343. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
1344. Please help me if you could - (reply: 5)
1345. Tm caculation for different PCRs?? - new, confused, and frustrated beginner (reply: 3)
1346. absolute quantification - (reply: 4)
1347. The Case of a Missing Band: PCR Issue - (reply: 4)
1348. Log 10 fold serial dilutions - (reply: 2)
1349. paranormal qpcr amplification activity - melting curves (reply: 3)
1350. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)