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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1321. a simple primer problem - (reply: 5)
1322. Failure to introduce mutations using Overlap PCR - (reply: 3)
1323. no or poor amplification - (reply: 1)
1324. Primers from 3'UTR - (reply: 2)
1325. Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
1326. Designing primers with ESTs - (reply: 2)
1327. Ct variation/Ct limit/rē values/efficiency errors - (reply: 2)
1328. Probe manufacturers, who makes best probes? - Where do you get your probes and are you happy with them? (reply: 3)
1329. Real Time vs Traditional PCR results - (reply: 1)
1330. The problems to identify mouse genotype with PCR - (reply: 1)
1331. non specific amplification - DNA amplification by PCR (reply: 1)
1332. RNA Extraction - Extraction of Yeast RNA from mice blood (reply: 1)
1333. Large Difference between Ct of 18s rRNA and Target Gene - (reply: 2)
1334. PCR has stopped working - (reply: 7)
1335. PCR negative control contamination - (reply: 8)
1336. Real-Time PCR - Gene expression (reply: 2)
1337. PCR efficiency in real timePCR - (reply: 5)
1338. Plate setup for qRT-PCR - Too many samples for one plate... (reply: 1)
1339. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
1340. QPCR analysis with various # of samples from 1 patient? - (reply: 1)
1341. Drastic Decrease in PCR product yield - (reply: 2)
1342. Contamination? - (reply: 1)
1343. multiplexing taqman miRNA reverse transcription - (reply: 2)
1344. ligation problem - (reply: 3)
1345. Analyzing qPCR results - (reply: 1)
1346. resources on PCR principles & technique - (reply: 1)
1347. inconsistent results - easy 16S amplifcation is not so easy - (reply: 1)
1348. Problem cDNA synthesis/ 2 step RT - (reply: 6)
1349. High MW PCR band seen...help needed - (reply: 7)
1350. QPCR - Primer and Probe question - QPCR (reply: 2)