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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1321. Primer Concentration to lower Ct - (reply: 1)
1322. Primer Concentration Help - (reply: 4)
1323. Can normal sequencing services sequence >1kb product? - sequencing (reply: 1)
1324. who got a manual of ABI7500 PCR instrument ? - how to operate this instument ? (reply: 4)
1325. Primer Degradation - What exactly happens? (reply: 1)
1326. Smearing on ategarose gel of real time pcr product??? - (reply: 1)
1327. PCR/DNA Extraction Problem - (reply: 5)
1328. Primers in 2 exons - (reply: 2)
1329. NTC appear in real time pcr - (reply: 6)
1330. RNA solutions - Calculating concentrations (reply: 9)
1331. PCR of AT-rich DNA - (reply: 1)
1332. NTP mix (with dUTP instead of dTTP) in sybr green mix - (reply: 1)
1333. TBE Buffer? - (reply: 4)
1334. Overlapping PCR - really need help ! - (reply: 5)
1335. gel documentation sysytem - (reply: 7)
1336. internal standard - (reply: 5)
1337. Checking genomic contamination - different ways (reply: 4)
1338. Probe or Primer info for bovine AQP-1 - (reply: 6)
1339. PCR with Platinum Taq - product yield issues - (reply: 3)
1340. real time PCR - (reply: 7)
1341. very high and inconsistent Cts for the house keeping gene - (reply: 4)
1342. Standard curves, are they necessary every time we run an experiment? - (reply: 6)
1343. Sample handling for RNA extraction - (reply: 1)
1344. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
1345. Normalising RNA concentrations - Help (reply: 4)
1346. How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)
1347. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
1348. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
1349. Having abnormal dissociation curve when performing qPCR - (reply: 3)
1350. Differences between primers for real-time PCR and RT-PCR - (reply: 6)