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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1321. LINE1 OFR2 Primer Design - (reply: 1)
1322. rna isolation manually - (reply: 2)
1323. phosphorylation of primers using PNK - (reply: 1)
1324. RT-PCR contamination issue - (reply: 3)
1325. What's the longest overhanging primer seq. you have used? - (reply: 2)
1326. Primer Concentration to lower Ct - (reply: 1)
1327. Primer Concentration Help - (reply: 4)
1328. Can normal sequencing services sequence >1kb product? - sequencing (reply: 1)
1329. who got a manual of ABI7500 PCR instrument ? - how to operate this instument ? (reply: 4)
1330. Primer Degradation - What exactly happens? (reply: 1)
1331. Smearing on ategarose gel of real time pcr product??? - (reply: 1)
1332. PCR/DNA Extraction Problem - (reply: 5)
1333. Primers in 2 exons - (reply: 2)
1334. NTC appear in real time pcr - (reply: 6)
1335. RNA solutions - Calculating concentrations (reply: 9)
1336. PCR of AT-rich DNA - (reply: 1)
1337. NTP mix (with dUTP instead of dTTP) in sybr green mix - (reply: 1)
1338. TBE Buffer? - (reply: 4)
1339. Overlapping PCR - really need help ! - (reply: 5)
1340. gel documentation sysytem - (reply: 7)
1341. internal standard - (reply: 5)
1342. Checking genomic contamination - different ways (reply: 4)
1343. Probe or Primer info for bovine AQP-1 - (reply: 6)
1344. PCR with Platinum Taq - product yield issues - (reply: 3)
1345. real time PCR - (reply: 7)
1346. very high and inconsistent Cts for the house keeping gene - (reply: 4)
1347. Standard curves, are they necessary every time we run an experiment? - (reply: 6)
1348. Sample handling for RNA extraction - (reply: 1)
1349. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
1350. Normalising RNA concentrations - Help (reply: 4)