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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1321. Mutagenesis woes - (reply: 3)
1322. KpnI Restriction enzyme - how to detect the SNP (reply: 1)
1323. intron/exon spanning primers - (reply: 1)
1324. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
1325. PCR product for sequencing - sample storage?? (reply: 2)
1326. Absolute quantification using Real Time PCR - (reply: 2)
1327. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
1328. High Ct value - what to do next? - (reply: 4)
1329. Biorad Mini Opticon - can use any 0.2ml tubes or Biorad only? (reply: 1)
1330. RT-PCR Gel - Ladder looks terrible and product is fuzzy! (reply: 1)
1331. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
1332. Designing Primers for multiple Isoforms - (reply: 1)
1333. GAPDH CT lower than target gene CT - (reply: 2)
1334. standards for absolute quantification qPCR - (reply: 1)
1335. RT-PCR standard curve dilutions - (reply: 2)
1336. more than one band - (reply: 1)
1337. Relative gene expression - Newbie at RT-qPCR! (reply: 3)
1338. qPCR, RT- Control - (reply: 2)
1339. Rt-PCR problem !with 2.5 kb gene - (reply: 3)
1340. real time pcr melt curve and primer efficiency problem - (reply: 3)
1341. real time PCR - base line not zero anymore! (reply: 5)
1342. Pre-amplification of template for qPCR - (reply: 1)
1343. Efficiency estimation using a window of linearity - (reply: 3)
1344. Software for analysis of raw real-time flouresence data - Analysis of tab delimited text flouresence v cycle data (reply: 7)
1345. Real time PCR - Standard curve construction (reply: 2)
1346. issue with taqman MGB probes - (reply: 2)
1347. PCR product dimer issue - (reply: 24)
1348. PCR additives Formamide - Formamide which one? (reply: 4)
1349. RNA from Zebrafish - (reply: 3)
1350. Polymerase Chain Reaction (PCR) - Anylyzing my PCR gel (reply: 6)