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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1321. RT-PCR contamination issue - (reply: 3)
1322. What's the longest overhanging primer seq. you have used? - (reply: 2)
1323. Primer Concentration to lower Ct - (reply: 1)
1324. Primer Concentration Help - (reply: 4)
1325. Can normal sequencing services sequence >1kb product? - sequencing (reply: 1)
1326. who got a manual of ABI7500 PCR instrument ? - how to operate this instument ? (reply: 4)
1327. Primer Degradation - What exactly happens? (reply: 1)
1328. Smearing on ategarose gel of real time pcr product??? - (reply: 1)
1329. PCR/DNA Extraction Problem - (reply: 5)
1330. Primers in 2 exons - (reply: 2)
1331. NTC appear in real time pcr - (reply: 6)
1332. RNA solutions - Calculating concentrations (reply: 9)
1333. PCR of AT-rich DNA - (reply: 1)
1334. NTP mix (with dUTP instead of dTTP) in sybr green mix - (reply: 1)
1335. TBE Buffer? - (reply: 4)
1336. Overlapping PCR - really need help ! - (reply: 5)
1337. gel documentation sysytem - (reply: 7)
1338. internal standard - (reply: 5)
1339. Checking genomic contamination - different ways (reply: 4)
1340. Probe or Primer info for bovine AQP-1 - (reply: 6)
1341. PCR with Platinum Taq - product yield issues - (reply: 3)
1342. real time PCR - (reply: 7)
1343. very high and inconsistent Cts for the house keeping gene - (reply: 4)
1344. Standard curves, are they necessary every time we run an experiment? - (reply: 6)
1345. Sample handling for RNA extraction - (reply: 1)
1346. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
1347. Normalising RNA concentrations - Help (reply: 4)
1348. How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)
1349. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
1350. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)