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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1321. With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
1322. Chlamydia PCR cycling conditions - (reply: 1)
1323. On-unit screen or PC…which is better? - (reply: 12)
1324. Primers going "loopy" - (reply: 2)
1325. Biotinylated! - brief explanaion (reply: 2)
1326. what do i say?! - (reply: 2)
1327. Smearing on gel in PCR products - (reply: 5)
1328. 260/230 ratio: how low is good for running PCRs? - (reply: 2)
1329. introducing alanine mutations - (reply: 1)
1330. Hematin - calculation (reply: 1)
1331. How to generate sticky ends? - (reply: 3)
1332. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
1333. PROBLEM WITH MELTING CURVES - i realy need help (reply: 2)
1334. Relative RT-PCR, multiplate and calibration - (reply: 4)
1335. Trying to measure small levels of mRNA - (reply: 3)
1336. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
1337. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
1338. Relative RT-PCR - (reply: 2)
1339. Relative real time RT PCR - (reply: 2)
1340. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
1341. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
1342. IQ5 and sybr/FAM - (reply: 3)
1343. a simple primer problem - (reply: 5)
1344. Failure to introduce mutations using Overlap PCR - (reply: 3)
1345. no or poor amplification - (reply: 1)
1346. Primers from 3'UTR - (reply: 2)
1347. Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
1348. Designing primers with ESTs - (reply: 2)
1349. Ct variation/Ct limit/r˛ values/efficiency errors - (reply: 2)
1350. Probe manufacturers, who makes best probes? - Where do you get your probes and are you happy with them? (reply: 3)