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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1321. Log 10 fold serial dilutions - (reply: 2)
1322. paranormal qpcr amplification activity - melting curves (reply: 3)
1323. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
1324. PCR template concentration - (reply: 6)
1325. PCR efficiency calculated by Linreg - (reply: 3)
1326. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
1327. Stability of genomic DNA in 1x PBS - (reply: 2)
1328. primer design by generunner DG - (reply: 1)
1329. Calculation of Tm of amplicon when using SYBR Green - (reply: 3)
1330. degenerate pcr - (reply: 1)
1331. DNA degradation - (reply: 1)
1332. DNase 1 treratment of RNA - (reply: 11)
1333. Secondary structure in real time - (reply: 1)
1334. primers with fluoresent dye PCR cycle question - (reply: 4)
1335. House keeping gene question? - Thanks for any help! (reply: 1)
1336. Questions about Tween 20 - (reply: 1)
1337. General qRT-PCR question - (reply: 3)
1338. qPCR stats problem - Vandesompele's, DDct or other methods?? (reply: 1)
1339. Taqman probe not easily synthesized - What sequence features make probe synthesis difficult? (reply: 3)
1340. Smears on PCR products - (reply: 4)
1341. Dumb Reverse Transcription PCR Question - (reply: 2)
1342. qPCR absolute/relative quantification method question - (reply: 4)
1343. PCR-amplicon too short - (reply: 1)
1344. Reverse transcription PCR - (reply: 1)
1345. PCR/RT-PCR beads - (reply: 2)
1346. High Amplification Efficiency in Std. Curve - (reply: 5)
1347. Run PCR amplifications in agarose gels - (reply: 3)
1348. Problems with validating primers and low expression genes - (reply: 3)
1349. Compare different qPCR runs - really need help (reply: 8)
1350. General real time questions. - (reply: 1)