Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
241. Primers have worked well but now getting primer dimers? - (reply: 2)
242. I cannot design primers on exon-exon junction - (reply: 2)
243. DNA Quantification of PCR Products - (reply: 2)
244. In 2^ minus ddCt method, why is the "minus" sign? - (reply: 1)
245. Problem for PCR - (reply: 9)
246. Confused about my CT values - (reply: 1)
247. Designing primers in UTRs - (reply: 1)
248. Multiplex PCR - (reply: 1)
249. primer design@ buy? - (reply: 2)
250. High Ct/Cq values in my NO-RT and NTC samples - (reply: 3)
251. Dissolving DNA Oligos - (reply: 2)
252. Trouble with overlap extension pcr - (reply: 3)
253. copies per ul to copies per gram tissue - (reply: 2)
254. designing primers( selecting target sequence/amplicon design) - (reply: 3)
255. Question about the RT PCR - (reply: 3)
256. PCR ready mixes with long shelf lives - (reply: 4)
257. Problem with repeatability of the standard curve - (reply: 3)
258. Influenza virus - (reply: 3)
259. Defining standards in qPCR softwares - (reply: 3)
260. How to design primer to amplify genomic DNA? - (reply: 3)
261. Overlap PCR, need help - (reply: 11)
262. Internal control for miRNA RT-PCR - (reply: 1)
263. Primers - (reply: 1)
264. Primers mix - (reply: 2)
265. degenerate bases in my sequences - (reply: 1)
266. Need help with dCAPS pcr, seeing huge bands on gel - (reply: 1)
267. How do I make GTE buffer for alkaline lysis? - (reply: 2)
268. Having problem with primers for qPCR - (reply: 4)
269. strange raw data - - (reply: 3)
270. need a method for oligonucleotide visualization - (reply: 5)