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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
241. RNA concentration to start RT reaction - (reply: 1)
242. Random vs oligo primer in preamplification RT - (reply: 1)
243. Smart Cycler II software and Absolute quantification help - (reply: 1)
244. cDNA amount in qPCR - (reply: 2)
245. mRNA integrity for qPCR - (reply: 6)
246. Strange control contamination in cDNA synthesis - (reply: 2)
247. how does polymerase stop at the required length - (reply: 1)
248. Perfect dilution curve, but double peak melting curve on SYBR qPCR - (reply: 7)
249. Real-time efficiency question - (reply: 1)
250. Selective removal of standards post qPCR Run - (reply: 1)
251. Primer concentration - stupid question - (reply: 3)
252. Primer Design, help im New - (reply: 4)
253. qPCR question (no results?) - (reply: 1)
254. Artificial RNA as normalizer (HK gene) - (reply: 1)
255. Sensitivity of RT-PCR and qPCR - (reply: 4)
256. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
257. real time PCR trouble - (reply: 3)
258. removal of ethanol in PCR product purification - (reply: 5)
259. Crazy real time PCR curve - (reply: 4)
260. High fidelity PCR trouble shooting - (reply: 2)
261. Reproducible Non-Specific PCR Product - (reply: 2)
262. How to prepare serial dilutions of RNA or cDNA - (reply: 3)
263. Are these primer products good enough for qPCR? - (reply: 3)
264. mtDNA to nDNA Ratio gDNA - qPCR - (reply: 1)
265. Can't get PCR with large overhang primers to work - (reply: 8)
266. Primer Efficiency across runs - (reply: 1)
267. Question about pipet tips for PCR and rtPCR - (reply: 4)
268. Taqman Probe sequence problem - (reply: 4)
269. UNG in PCR - (reply: 1)
270. Cause of random samples failing PCR? - (reply: 2)