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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
241. RNA concentration suddenly drops - (reply: 3)
242. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
243. PCR to get 10kbp product - (reply: 4)
244. site-directed mutagenesis primer Tm - (reply: 4)
245. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
246. RNA concentration to start RT reaction - (reply: 1)
247. Random vs oligo primer in preamplification RT - (reply: 1)
248. Smart Cycler II software and Absolute quantification help - (reply: 1)
249. cDNA amount in qPCR - (reply: 2)
250. mRNA integrity for qPCR - (reply: 6)
251. Strange control contamination in cDNA synthesis - (reply: 2)
252. how does polymerase stop at the required length - (reply: 1)
253. Perfect dilution curve, but double peak melting curve on SYBR qPCR - (reply: 7)
254. Real-time efficiency question - (reply: 1)
255. Selective removal of standards post qPCR Run - (reply: 1)
256. Primer concentration - stupid question - (reply: 3)
257. Primer Design, help im New - (reply: 4)
258. qPCR question (no results?) - (reply: 1)
259. Artificial RNA as normalizer (HK gene) - (reply: 1)
260. Sensitivity of RT-PCR and qPCR - (reply: 4)
261. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
262. real time PCR trouble - (reply: 3)
263. removal of ethanol in PCR product purification - (reply: 5)
264. Crazy real time PCR curve - (reply: 4)
265. High fidelity PCR trouble shooting - (reply: 2)
266. Reproducible Non-Specific PCR Product - (reply: 2)
267. How to prepare serial dilutions of RNA or cDNA - (reply: 3)
268. Are these primer products good enough for qPCR? - (reply: 3)
269. mtDNA to nDNA Ratio gDNA - qPCR - (reply: 1)
270. Can't get PCR with large overhang primers to work - (reply: 8)