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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
241. Please please help me with my Phusion PCR. - (reply: 5)
242. Nanodrop vs Picogreen to quantify post-PCR product - (reply: 3)
243. Need no RT control for all genes? - (reply: 1)
244. Different annealing temp between HKG and target gene - (reply: 2)
245. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
246. help in long pcr - (reply: 1)
247. Reselling a supermix/enzyme in a commercial diagnostic kit - (reply: 2)
248. Small yield of RNA after on column DNAs digestion , Why - (reply: 3)
249. PCR inhibitor in template DNA - (reply: 3)
250. Why when disigning primers for RTPCR, you need to chose a region where introns a - (reply: 1)
251. Problem with Real-time PCR results analysis - (reply: 1)
252. Rotor-Gene 6000 data analysis - (reply: 1)
253. PCR from protozoa DNA - (reply: 3)
254. Ligation-mediated PCR: Vent Polymerase, Why? - (reply: 7)
255. tool for comparing many primers pairs - (reply: 4)
256. PCR that leads to protein synthesis - (reply: 18)
257. Real-time PCR does not work, while the conventional PCR (with same conditions an - (reply: 3)
258. Use of DMSO in General PCR - (reply: 1)
259. PCR product size confusion - (reply: 3)
260. Concentration specification in PCR - (reply: 3)
261. help us understand the run - (reply: 1)
262. Different MOI in comparison experiment - (reply: 3)
263. How to determine the size of gene to amplify? - (reply: 1)
264. Guanidine isothiocyanate in PCR - (reply: 1)
265. Untreated samples negative, how to analyze fold change? - (reply: 1)
266. Primers have worked well but now getting primer dimers? - (reply: 2)
267. I cannot design primers on exon-exon junction - (reply: 2)
268. DNA Quantification of PCR Products - (reply: 2)
269. In 2^ minus ddCt method, why is the "minus" sign? - (reply: 1)
270. Problem for PCR - (reply: 9)