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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
241. Application of RNase - (reply: 3)
242. i need help with virus pcr - (reply: 2)
243. Primer Efficiency - (reply: 2)
244. data analysis for RT_PCR - (reply: 4)
245. Experimental set up for qRT PCR - (reply: 13)
246. What temp the lid of the thermocycler must be set? - (reply: 1)
247. How should I optimize my PCR - (reply: 2)
248. Real time data analysis using 2 machines - (reply: 3)
249. Second round of PCR after gel extraction fails miserably - (reply: 3)
250. urgent help plz with RT-PCR - (reply: 3)
251. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
252. LightCycler 480 Melting Curve Assay - (reply: 1)
253. Problems with two step RT-PCR - (reply: 2)
254. Spliced and unspliced form of a gene - (reply: 3)
255. primer dilutions - (reply: 4)
256. Genotyping PCR primers - (reply: 1)
257. Standard curve using plasmid - (reply: 1)
258. How to compare these results? Please comment. - (reply: 5)
259. Primers too dilute - (reply: 1)
260. Amplification curves jagged - (reply: 1)
261. ddCT issue: no endogenous expression in control. - (reply: 4)
262. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
263. No PCR product in site-directed mutagenesis - (reply: 4)
264. Giardia/Crypto real time PCR late amplification - (reply: 1)
265. How to dilute the template DNA for PCR - (reply: 7)
266. Desired length of overlap region for OE-PCR - (reply: 5)
267. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
268. Need help for PCR - (reply: 5)
269. scorpion probe - (reply: 8)
270. Too low Ct value for reference gene !!! - (reply: 3)