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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
241. hybridization probe - (reply: 1)
242. How to get rid of bands in PCR negative control - (reply: 10)
243. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
244. standard curve using genomic dna - (reply: 1)
245. PCR problem - (reply: 2)
246. defining ratio of alternative spliced gene with qPCR - (reply: 14)
247. Poor efficiency standard curve - (reply: 2)
248. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
249. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
250. PCR gene specific amplification problem - (reply: 3)
251. Failure SYBRGREEN PCR - (reply: 4)
252. Pfaffl Method for relative - (reply: 4)
253. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
254. experiences with NuPCR from Illumina? - (reply: 1)
255. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
256. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
257. 100 ng RNA for cDNA synthesis - (reply: 4)
258. Inexplicable qPCR failure in single wells - (reply: 12)
259. negative control well 300 bp band - (reply: 3)
260. Reference gene for normalisation - for different growth rates - (reply: 3)
261. Strange amplification plots with high Ct variability - (reply: 1)
262. How big a role does mixing play in PCR - (reply: 1)
263. Melting curve is irregular for primer optimization - (reply: 5)
264. Designing primers for ABO blood groups - (reply: 1)
265. Methylight Results Analysis - (reply: 1)
266. GAPDH Ct value - (reply: 4)
267. dissociation curves - (reply: 1)
268. RT-PCR primer design - (reply: 7)
269. How to amplify very short PCR template - (reply: 4)
270. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)