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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
241. TaqMan CN assay - control sample duplicated - (reply: 1)
242. qPCR - no amplification curve but suitable melting curve - (reply: 3)
243. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
244. Basic question about qPCR and endogenous RNA control. - (reply: 4)
245. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
246. something very basic, why called "event-specific detection" - (reply: 1)
247. skip 65 degrees protocol of Reverse transcription - (reply: 4)
248. Mystery in my PCR - (reply: 5)
249. Whole genome amplification from cDNA? - (reply: 4)
250. What do you use to check primer secondary structure? - (reply: 3)
251. CT of qPCR - (reply: 3)
252. Need help with my real time RT-PCR Plate Set up - (reply: 8)
253. PCR product as template for in vitro transcription - (reply: 1)
254. Sybr Green vs Taqman -- a practical approach - (reply: 2)
255. Problem with 3 step PCR - (reply: 3)
256. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
257. ARMS-PCR - (reply: 1)
258. Scaling up PCR to get more DNA - (reply: 5)
259. RNA concentration suddenly drops - (reply: 3)
260. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
261. PCR to get 10kbp product - (reply: 4)
262. site-directed mutagenesis primer Tm - (reply: 4)
263. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
264. RNA concentration to start RT reaction - (reply: 1)
265. Random vs oligo primer in preamplification RT - (reply: 1)
266. Smart Cycler II software and Absolute quantification help - (reply: 1)
267. cDNA amount in qPCR - (reply: 2)
268. mRNA integrity for qPCR - (reply: 6)
269. Strange control contamination in cDNA synthesis - (reply: 2)
270. how does polymerase stop at the required length - (reply: 1)