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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
241. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
242. ARMS-PCR - (reply: 1)
243. Scaling up PCR to get more DNA - (reply: 5)
244. RNA concentration suddenly drops - (reply: 3)
245. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
246. PCR to get 10kbp product - (reply: 4)
247. site-directed mutagenesis primer Tm - (reply: 4)
248. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
249. RNA concentration to start RT reaction - (reply: 1)
250. Random vs oligo primer in preamplification RT - (reply: 1)
251. Smart Cycler II software and Absolute quantification help - (reply: 1)
252. cDNA amount in qPCR - (reply: 2)
253. mRNA integrity for qPCR - (reply: 6)
254. Strange control contamination in cDNA synthesis - (reply: 2)
255. how does polymerase stop at the required length - (reply: 1)
256. Perfect dilution curve, but double peak melting curve on SYBR qPCR - (reply: 7)
257. Real-time efficiency question - (reply: 1)
258. Selective removal of standards post qPCR Run - (reply: 1)
259. Primer concentration - stupid question - (reply: 3)
260. Primer Design, help im New - (reply: 4)
261. qPCR question (no results?) - (reply: 1)
262. Artificial RNA as normalizer (HK gene) - (reply: 1)
263. Sensitivity of RT-PCR and qPCR - (reply: 4)
264. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
265. real time PCR trouble - (reply: 3)
266. removal of ethanol in PCR product purification - (reply: 5)
267. Crazy real time PCR curve - (reply: 4)
268. High fidelity PCR trouble shooting - (reply: 2)
269. Reproducible Non-Specific PCR Product - (reply: 2)
270. How to prepare serial dilutions of RNA or cDNA - (reply: 3)