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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1. how to calculate amount of cDNA using RT-PCR - (reply: 2)
2. Preservation of human tissue for bacterial mRNA isolation from it later - (reply: 2)
3. qPCR uniformity test - (reply: 1)
4. NEB TM CALCULATOR PRIMER CONCENTRATION - (reply: 2)
5. primer design for a gene - (reply: 2)
6. need help setting up reverse transcriptase qPCR - (reply: 3)
7. Problems regarding amplification of my gene - (reply: 3)
8. Overlapping PCR, need help - (reply: 5)
9. How does RT-PCR work? - (reply: 5)
10. Denatured plasmid for pcr - (reply: 1)
11. Possible primer dimer problem..Need solution!!! - (reply: 2)
12. When to dilute samples for qPCR - (reply: 2)
13. Some info. on PCR products stability w/respect to shipping - (reply: 3)
14. 3' RACE troubles - (reply: 2)
15. haeting RNA before electrophoresis - (reply: 1)
16. PCR Products not run well in the GEL - (reply: 1)
17. Qubit Nanodrop Quantifications - (reply: 1)
18. Decontaminating DNA - Effective Means? - (reply: 4)
19. Consistancy issue with PCR block - (reply: 1)
20. Queries regarding PCR - (reply: 3)
21. Identification of Listeria monocytogenes - (reply: 1)
22. Calculation of Taq Polymerase - (reply: 3)
23. Best Taq for colony PCR? - (reply: 5)
24. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 6)
25. How to test my milleq water in q PCR about contaminants - (reply: 3)
26. different amount of cDNA in qRT-PCR for target and reference genes - (reply: 3)
27. real time PCR primer design - (reply: 1)
28. Plasmid Linearization Problems - (reply: 9)
29. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
30. Increasing RNA yield from very small tissues - (reply: 3)