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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1. Decontaminating DNA - Effective Means? - (reply: 2)
2. Consistancy issue with PCR block - (reply: 1)
3. Queries regarding PCR - (reply: 3)
4. Identification of Listeria monocytogenes - (reply: 1)
5. Calculation of Taq Polymerase - (reply: 3)
6. Best Taq for colony PCR? - (reply: 5)
7. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 5)
8. different amount of cDNA in qRT-PCR for target and reference genes - (reply: 3)
9. real time PCR primer design - (reply: 1)
10. Plasmid Linearization Problems - (reply: 9)
11. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
12. Increasing RNA yield from very small tissues - (reply: 3)
13. Degenerated Primers and their problems - (reply: 1)
14. Interpreting primer BLAST scores for self-complementary - (reply: 2)
15. Testing primers on 'unknown' tissue - (reply: 1)
16. Second Hand Roche Lightcycler II maching - to buy or not to buy - (reply: 1)
17. nested PCR for low viral load- HBV patient sample - (reply: 2)
18. Finding an answer as to why I have Tm but no Ct - (reply: 6)
19. Amplification in HK gene but not for target gene - (reply: 3)
20. Missing DNA Target and Effect on Non-Specific Amplicons - (reply: 1)
21. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
22. how to make this reaction? - (reply: 1)
23. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
24. How long can I store at - 30C my PCR mix? - (reply: 6)
25. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
26. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
27. RT-PCR help - (reply: 2)
28. Strange signals in the NTC - (reply: 1)
29. False positive - help - (reply: 3)
30. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)