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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1. How to optimize a master mix kit? - (reply: 7)
2. pcr primer design - (reply: 4)
3. Please help (primer/ probe final volume calculations) - (reply: 1)
4. Primer design with EcoRI ends - (reply: 2)
5. qPCR normalization - (reply: 4)
6. Reference Gene/HKG- every plate - (reply: 2)
7. qPCR standard for lipase genes - (reply: 2)
8. PCR with overlapping primers only (primer extention) - (reply: 3)
9. Random chuck of nonsense in middle of PCR - (reply: 6)
10. Campylobacter jejuni qPCR targeting VS1 gene - (reply: 2)
11. RT-PCR analysis - (reply: 1)
12. Newbie to qPCR - (reply: 4)
13. real time qPCR standard range plasmid - (reply: 3)
14. New to PCR - (reply: 3)
15. How can I check the quality of bacterial primers and their specificity on blast? - (reply: 1)
16. Who can advise me a kit that would allow me to purify amplicons from quantitativ - (reply: 3)
17. 3-primer PCR Genotyping - problem in hemizygotes - (reply: 2)
18. t-test & qRT-PCR - (reply: 1)
19. Primer design - (reply: 1)
20. What % of colon cancer patients does a general colon PCR array detect - (reply: 1)
21. Primer non specific binding sites - (reply: 7)
22. Degenerate primers - (reply: 8)
23. Can you use same samples for real time pcr 2nd time? - (reply: 2)
24. Absolute quant with TaqMan - Different RT & different plates? - (reply: 1)
25. Staphylococcus aureus colony PCR - (reply: 1)
26. The slightly bigger band dillema. - (reply: 2)
27. PCR & Western Blot sample preperation - (reply: 5)
28. Representing Ct values above set Cycle baseline? - (reply: 1)
29. Y-linker transgene integration site PCR primer verification - (reply: 1)
30. Non especific band in PCR... - (reply: 2)