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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1261. Problems with qRT-PCR - Lots of unspecific products (reply: 2)
1262. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
1263. 2 bands or smear? 2-step RT-PCR - (reply: 1)
1264. 23S rRNA Primer - need primer sequence (reply: 1)
1265. Lowered extension temps with Phusion - (reply: 7)
1266. RT-PCR - problems with TaqMan PCR (reply: 2)
1267. qRT-PCR calculations - Averaging across biological replicates (reply: 2)
1268. Two annealing temps? - (reply: 4)
1269. Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
1270. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
1271. Small product amplification problems - (reply: 4)
1272. ROX and Rotorgene - using a mix with reference dye in Rotorgene (reply: 2)
1273. Freezing primers+SYBR green - (reply: 2)
1274. Tagged Primers - (reply: 5)
1275. no pcr amplification product - (reply: 9)
1276. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
1277. less steep curve and high Ct value! - (reply: 2)
1278. Question about site directed mutagenesis - (reply: 1)
1279. Amplfication of fungal DNA - (reply: 8)
1280. PCR problem - (reply: 4)
1281. mRNA level in transgene cell line or knocking down cell line - (reply: 3)
1282. differently behavioring replicates in real-time PCR - (reply: 1)
1283. Multiplex PCR - (reply: 1)
1284. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
1285. optimal template dna concentrations - (reply: 1)
1286. reconstitution of primers - (reply: 2)
1287. Mysterious PCR Contamination - (reply: 13)
1288. RT-PCR Help - Protocol provided (reply: 4)
1289. Endogenous control in bacterial RT-qPCR - (reply: 4)
1290. Mutagenesis PCR and undesired amplification - (reply: 2)