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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1261. DNase 1 treratment of RNA - (reply: 11)
1262. Secondary structure in real time - (reply: 1)
1263. primers with fluoresent dye PCR cycle question - (reply: 4)
1264. House keeping gene question? - Thanks for any help! (reply: 1)
1265. Questions about Tween 20 - (reply: 1)
1266. General qRT-PCR question - (reply: 3)
1267. qPCR stats problem - Vandesompele's, DDct or other methods?? (reply: 1)
1268. Taqman probe not easily synthesized - What sequence features make probe synthesis difficult? (reply: 3)
1269. Smears on PCR products - (reply: 4)
1270. Dumb Reverse Transcription PCR Question - (reply: 2)
1271. qPCR absolute/relative quantification method question - (reply: 4)
1272. PCR-amplicon too short - (reply: 1)
1273. Reverse transcription PCR - (reply: 1)
1274. PCR/RT-PCR beads - (reply: 2)
1275. High Amplification Efficiency in Std. Curve - (reply: 5)
1276. Run PCR amplifications in agarose gels - (reply: 3)
1277. Problems with validating primers and low expression genes - (reply: 3)
1278. Compare different qPCR runs - really need help (reply: 8)
1279. General real time questions. - (reply: 1)
1280. Analysis HELP!!!! - (reply: 1)
1281. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
1282. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
1283. Mutagenesis woes - (reply: 3)
1284. KpnI Restriction enzyme - how to detect the SNP (reply: 1)
1285. intron/exon spanning primers - (reply: 1)
1286. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
1287. PCR product for sequencing - sample storage?? (reply: 2)
1288. Absolute quantification using Real Time PCR - (reply: 2)
1289. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
1290. High Ct value - what to do next? - (reply: 4)