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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1261. homemade mastermix for qPCR - any reason not to make a lot and freeze - (reply: 9)
1262. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
1263. qPCR for telomere length measurement - efficiency issues - complicated monochrome multiplex assay with SYBR Green (reply: 40)
1264. Nested PCR question - (reply: 1)
1265. High Tm on primers - cannot get a product - (reply: 8)
1266. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
1267. Help with Multiplex Nested PCR - (reply: 2)
1268. Relative quantification analysis Ct values & 2-ΔΔCT method - samples which do not amplify (reply: 5)
1269. trembling dissociation curves in HRM assays - (reply: 3)
1270. Fluorescein storage? - (reply: 2)
1271. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
1272. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
1273. TELO2 & GAPDH - (reply: 2)
1274. PCR -No band formation - (reply: 1)
1275. 3' race - (reply: 1)
1276. PCR AMPLIFICATION - (reply: 1)
1277. internal control for genomic DNA - (reply: 1)
1278. qPCR Data Analysis - Software (reply: 3)
1279. Gene expression confusion - (reply: 5)
1280. real time pcr need advise - (reply: 2)
1281. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
1282. ChIP-qPCR shift in melt curve - (reply: 4)
1283. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
1284. use of housekeeping gene in RT PCR chIP - (reply: 3)
1285. what is the order you follow in the run ??? - in order to make the contamination risk the least (reply: 3)
1286. DNA pooling for PCR - Saving money PCR (reply: 7)
1287. Real Time PCR Standard curves - How many are required??? (reply: 1)
1288. pcr-rflp - (reply: 2)
1289. PCR need some help - (reply: 4)
1290. problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)