Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1261. Relative quantification analysis Ct values & 2-ΔΔCT method - samples which do not amplify (reply: 5)
1262. trembling dissociation curves in HRM assays - (reply: 3)
1263. Fluorescein storage? - (reply: 2)
1264. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
1265. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
1266. TELO2 & GAPDH - (reply: 2)
1267. PCR -No band formation - (reply: 1)
1268. 3' race - (reply: 1)
1269. PCR AMPLIFICATION - (reply: 1)
1270. internal control for genomic DNA - (reply: 1)
1271. qPCR Data Analysis - Software (reply: 3)
1272. Gene expression confusion - (reply: 5)
1273. real time pcr need advise - (reply: 2)
1274. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
1275. ChIP-qPCR shift in melt curve - (reply: 4)
1276. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
1277. use of housekeeping gene in RT PCR chIP - (reply: 3)
1278. what is the order you follow in the run ??? - in order to make the contamination risk the least (reply: 3)
1279. DNA pooling for PCR - Saving money PCR (reply: 7)
1280. Real Time PCR Standard curves - How many are required??? (reply: 1)
1281. pcr-rflp - (reply: 2)
1282. PCR need some help - (reply: 4)
1283. problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)
1284. Left polymerase out overnight - Will it still work? (reply: 1)
1285. very low expression of a sample vs standard curve efficiency - (reply: 1)
1286. Primer Reconstitution--does temperature matter? - (reply: 5)
1287. TaqMan Gene Expression Assays - Low expression/ Ct values (reply: 2)
1288. Gradient PCR works, but single temperature does not! HELP!! - (reply: 5)
1289. Oligonucleotide degradation - (reply: 1)
1290. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)