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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1261. Primers in 2 exons - (reply: 2)
1262. NTC appear in real time pcr - (reply: 6)
1263. RNA solutions - Calculating concentrations (reply: 9)
1264. PCR of AT-rich DNA - (reply: 1)
1265. NTP mix (with dUTP instead of dTTP) in sybr green mix - (reply: 1)
1266. TBE Buffer? - (reply: 4)
1267. Overlapping PCR - really need help ! - (reply: 5)
1268. gel documentation sysytem - (reply: 7)
1269. internal standard - (reply: 5)
1270. Checking genomic contamination - different ways (reply: 4)
1271. Probe or Primer info for bovine AQP-1 - (reply: 6)
1272. PCR with Platinum Taq - product yield issues - (reply: 3)
1273. real time PCR - (reply: 7)
1274. very high and inconsistent Cts for the house keeping gene - (reply: 4)
1275. Standard curves, are they necessary every time we run an experiment? - (reply: 6)
1276. Sample handling for RNA extraction - (reply: 1)
1277. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
1278. Normalising RNA concentrations - Help (reply: 4)
1279. How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)
1280. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
1281. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
1282. Having abnormal dissociation curve when performing qPCR - (reply: 3)
1283. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
1284. Please help me if you could - (reply: 5)
1285. Tm caculation for different PCRs?? - new, confused, and frustrated beginner (reply: 3)
1286. absolute quantification - (reply: 4)
1287. The Case of a Missing Band: PCR Issue - (reply: 4)
1288. Log 10 fold serial dilutions - (reply: 2)
1289. paranormal qpcr amplification activity - melting curves (reply: 3)
1290. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)