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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1261. Left polymerase out overnight - Will it still work? (reply: 1)
1262. very low expression of a sample vs standard curve efficiency - (reply: 1)
1263. Primer Reconstitution--does temperature matter? - (reply: 5)
1264. TaqMan Gene Expression Assays - Low expression/ Ct values (reply: 2)
1265. Gradient PCR works, but single temperature does not! HELP!! - (reply: 5)
1266. Oligonucleotide degradation - (reply: 1)
1267. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
1268. never get bands by pfu related enzymes - (reply: 2)
1269. Primer design for qPCR - (reply: 1)
1270. Primer-BLAST... - (reply: 4)
1271. reporting results of qPCR - (reply: 1)
1272. a question about 16s rRNA as reference in RT-qPCR - (reply: 1)
1273. PCR genomic DNA of high GC content - (reply: 6)
1274. question about qRT-PCR preparation - (reply: 1)
1275. GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
1276. qPCR efficiency calculation - (reply: 1)
1277. qPCR on chIP product - (reply: 3)
1278. Ghost contamination in RT-PCR - Mystery that i can't solve. Help. (reply: 1)
1279. PCR--11kb fragment - (reply: 4)
1280. primer design tips - (reply: 3)
1281. GroEL from Wolbachia - GroEL from Wolbachia (reply: 2)
1282. PCR with Biotin Incorporation - (reply: 2)
1283. Anyone uses plasmid to make standard curves? how to make adjustment b/w plates - real time (reply: 4)
1284. pcr product - hello all (reply: 1)
1285. how to save sybr green reagents? - (reply: 3)
1286. amplification of GroEL from Wolbachia! - amplification of GroEL from Wolbachia! (reply: 2)
1287. Forgot to stop reverse transcription reaction - (reply: 4)
1288. PCR with long and complex primers - (reply: 2)
1289. Phospholylation of primers - (reply: 1)
1290. Re-using PCR plates? - (reply: 2)