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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1261. Nested PCR question - (reply: 1)
1262. High Tm on primers - cannot get a product - (reply: 8)
1263. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
1264. Help with Multiplex Nested PCR - (reply: 2)
1265. Relative quantification analysis Ct values & 2-ΔΔCT method - samples which do not amplify (reply: 5)
1266. trembling dissociation curves in HRM assays - (reply: 3)
1267. Fluorescein storage? - (reply: 2)
1268. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
1269. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
1270. TELO2 & GAPDH - (reply: 2)
1271. PCR -No band formation - (reply: 1)
1272. 3' race - (reply: 1)
1273. PCR AMPLIFICATION - (reply: 1)
1274. internal control for genomic DNA - (reply: 1)
1275. qPCR Data Analysis - Software (reply: 3)
1276. Gene expression confusion - (reply: 5)
1277. real time pcr need advise - (reply: 2)
1278. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
1279. ChIP-qPCR shift in melt curve - (reply: 4)
1280. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
1281. use of housekeeping gene in RT PCR chIP - (reply: 3)
1282. what is the order you follow in the run ??? - in order to make the contamination risk the least (reply: 3)
1283. DNA pooling for PCR - Saving money PCR (reply: 7)
1284. Real Time PCR Standard curves - How many are required??? (reply: 1)
1285. pcr-rflp - (reply: 2)
1286. PCR need some help - (reply: 4)
1287. problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)
1288. Left polymerase out overnight - Will it still work? (reply: 1)
1289. very low expression of a sample vs standard curve efficiency - (reply: 1)
1290. Primer Reconstitution--does temperature matter? - (reply: 5)