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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1261. use of housekeeping gene in RT PCR chIP - (reply: 3)
1262. what is the order you follow in the run ??? - in order to make the contamination risk the least (reply: 3)
1263. DNA pooling for PCR - Saving money PCR (reply: 7)
1264. Real Time PCR Standard curves - How many are required??? (reply: 1)
1265. pcr-rflp - (reply: 2)
1266. PCR need some help - (reply: 4)
1267. problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)
1268. Left polymerase out overnight - Will it still work? (reply: 1)
1269. very low expression of a sample vs standard curve efficiency - (reply: 1)
1270. Primer Reconstitution--does temperature matter? - (reply: 5)
1271. TaqMan Gene Expression Assays - Low expression/ Ct values (reply: 2)
1272. Gradient PCR works, but single temperature does not! HELP!! - (reply: 5)
1273. Oligonucleotide degradation - (reply: 1)
1274. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
1275. never get bands by pfu related enzymes - (reply: 2)
1276. Primer design for qPCR - (reply: 1)
1277. Primer-BLAST... - (reply: 4)
1278. reporting results of qPCR - (reply: 1)
1279. a question about 16s rRNA as reference in RT-qPCR - (reply: 1)
1280. PCR genomic DNA of high GC content - (reply: 6)
1281. question about qRT-PCR preparation - (reply: 1)
1282. GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
1283. qPCR efficiency calculation - (reply: 1)
1284. qPCR on chIP product - (reply: 3)
1285. Ghost contamination in RT-PCR - Mystery that i can't solve. Help. (reply: 1)
1286. PCR--11kb fragment - (reply: 4)
1287. primer design tips - (reply: 3)
1288. GroEL from Wolbachia - GroEL from Wolbachia (reply: 2)
1289. PCR with Biotin Incorporation - (reply: 2)
1290. Anyone uses plasmid to make standard curves? how to make adjustment b/w plates - real time (reply: 4)