Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1261. General real time questions. - (reply: 1)
1262. Analysis HELP!!!! - (reply: 1)
1263. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
1264. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
1265. Mutagenesis woes - (reply: 3)
1266. KpnI Restriction enzyme - how to detect the SNP (reply: 1)
1267. intron/exon spanning primers - (reply: 1)
1268. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
1269. PCR product for sequencing - sample storage?? (reply: 2)
1270. Absolute quantification using Real Time PCR - (reply: 2)
1271. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
1272. High Ct value - what to do next? - (reply: 4)
1273. Biorad Mini Opticon - can use any 0.2ml tubes or Biorad only? (reply: 1)
1274. RT-PCR Gel - Ladder looks terrible and product is fuzzy! (reply: 1)
1275. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
1276. Designing Primers for multiple Isoforms - (reply: 1)
1277. GAPDH CT lower than target gene CT - (reply: 2)
1278. standards for absolute quantification qPCR - (reply: 1)
1279. RT-PCR standard curve dilutions - (reply: 2)
1280. more than one band - (reply: 1)
1281. Relative gene expression - Newbie at RT-qPCR! (reply: 3)
1282. qPCR, RT- Control - (reply: 2)
1283. Rt-PCR problem !with 2.5 kb gene - (reply: 3)
1284. real time pcr melt curve and primer efficiency problem - (reply: 3)
1285. real time PCR - base line not zero anymore! (reply: 5)
1286. Pre-amplification of template for qPCR - (reply: 1)
1287. Efficiency estimation using a window of linearity - (reply: 3)
1288. Software for analysis of raw real-time flouresence data - Analysis of tab delimited text flouresence v cycle data (reply: 7)
1289. Real time PCR - Standard curve construction (reply: 2)
1290. issue with taqman MGB probes - (reply: 2)