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1261. PCR contamination - (reply: 19)
1262. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
1263. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
1264. How to do HRM curve analyses with SYBR Green Dye? - How to do HRM curve analyses with SYBR Green Dye using ABI 7500 RT-PC? (reply: 5)
1265. PCR not working - No amplification - (reply: 5)
1266. Quantification of PCR products - (reply: 1)
1267. Comment: FYI be careful which program you use to analyze data from Bio-Rad therm - and be careful of the advice Bio-Rad offers (reply: 1)
1268. homemade mastermix for qPCR - any reason not to make a lot and freeze - (reply: 9)
1269. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
1270. qPCR for telomere length measurement - efficiency issues - complicated monochrome multiplex assay with SYBR Green (reply: 40)
1271. Nested PCR question - (reply: 1)
1272. High Tm on primers - cannot get a product - (reply: 8)
1273. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
1274. Help with Multiplex Nested PCR - (reply: 2)
1275. Relative quantification analysis Ct values & 2-ΔΔCT method - samples which do not amplify (reply: 5)
1276. trembling dissociation curves in HRM assays - (reply: 3)
1277. Fluorescein storage? - (reply: 2)
1278. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
1279. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
1280. TELO2 & GAPDH - (reply: 2)
1281. PCR -No band formation - (reply: 1)
1282. 3' race - (reply: 1)
1283. PCR AMPLIFICATION - (reply: 1)
1284. internal control for genomic DNA - (reply: 1)
1285. qPCR Data Analysis - Software (reply: 3)
1286. Gene expression confusion - (reply: 5)
1287. real time pcr need advise - (reply: 2)
1288. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
1289. ChIP-qPCR shift in melt curve - (reply: 4)
1290. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
1262. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
1263. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
1264. How to do HRM curve analyses with SYBR Green Dye? - How to do HRM curve analyses with SYBR Green Dye using ABI 7500 RT-PC? (reply: 5)
1265. PCR not working - No amplification - (reply: 5)
1266. Quantification of PCR products - (reply: 1)
1267. Comment: FYI be careful which program you use to analyze data from Bio-Rad therm - and be careful of the advice Bio-Rad offers (reply: 1)
1268. homemade mastermix for qPCR - any reason not to make a lot and freeze - (reply: 9)
1269. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
1270. qPCR for telomere length measurement - efficiency issues - complicated monochrome multiplex assay with SYBR Green (reply: 40)
1271. Nested PCR question - (reply: 1)
1272. High Tm on primers - cannot get a product - (reply: 8)
1273. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
1274. Help with Multiplex Nested PCR - (reply: 2)
1275. Relative quantification analysis Ct values & 2-ΔΔCT method - samples which do not amplify (reply: 5)
1276. trembling dissociation curves in HRM assays - (reply: 3)
1277. Fluorescein storage? - (reply: 2)
1278. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
1279. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
1280. TELO2 & GAPDH - (reply: 2)
1281. PCR -No band formation - (reply: 1)
1282. 3' race - (reply: 1)
1283. PCR AMPLIFICATION - (reply: 1)
1284. internal control for genomic DNA - (reply: 1)
1285. qPCR Data Analysis - Software (reply: 3)
1286. Gene expression confusion - (reply: 5)
1287. real time pcr need advise - (reply: 2)
1288. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
1289. ChIP-qPCR shift in melt curve - (reply: 4)
1290. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)