delta Ct values - (Dec/07/2010 )
I need to compare gene expression values of only healthy individuals using real time pcr. I now have the Ct values for the different individuals for the gene of interest as well as the house keeping gene. I was wondering if delta Ct is the method that i could use for this i.e. I calculate delta Ct by subtracting Ct of gene of interest from Ct of house keeping gene, and then use the formula =power(2,-delta Ct) in excel to calculate the change in expression. Further use ANOVA to graphically represent these results?
Any help would be highly appreciated.
Thanks in advance
Look up the whitepaper: http://www.integromics.com/WhitePaper_qPCR_Statistics and also Livak paper.