meaningless real time results - (Feb/15/2012 )
Today ı made Real time PCR for the first and I met a problem.
I try to generate ips cell with using yamanaka's factor. I gave them to my cell with lentiviral vectors but we haven' t been succesfull in formation of ips colonies. To see whether factor is inserted into our cell, we do real time pcr by using touchdown protocol for 5 genes ( oct4, klf4, sox2, c-myc, nanog). But our result was so meaningless. What can cause it? Pleas help me..
Can you explain more precisely what you mean by saying that your result was meaningless ? Do you mean you didn't see any change in gene expression, or that you had a bad primer efficiency, or melting curve,.....