Normalization factor - (Apr/06/2013 )
could you please suggest me some software/excel sheet/formula to calculate normlaization factor for qPCR data?
Maybe this helps:
A new mathematical model for relative quantification in real-time RT-PCR.
Nucleic Acids Res. 2001 May 1;29(9):e45
On this (by the way excellent) real time pcr tutorial website you can find the link to an excel sheet using the Pfaffl Method:
And here is the tutorial itself: