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Normalization factor - (Apr/06/2013 )


could you please suggest me some software/excel sheet/formula to calculate normlaization factor for qPCR data?

-Mad Researcher-

Maybe this helps:

A new mathematical model for relative quantification in real-time RT-PCR.
Pfaffl MW.
Nucleic Acids Res. 2001 May 1;29(9):e45


On this (by the way excellent) real time pcr tutorial website you can find the link to an excel sheet using the Pfaffl Method:

And here is the tutorial itself:


Thanks guys :)

-Mad Researcher-