Genbank, 5' or 3' - (Sep/26/2013 )
Hi, How do I figure out whether a sequence starts at the 5' or 3' end on Genbank? Thanks, I'm going crazy trying to find this out.
Unless it is explicitly shown otherwise, all sequence, everywhere, is shown 5' to 3'. Of course, if it is sequence of a double stranded piece of DNA, then there is also a reverse complement strand, which will (if written out) be in the 3' to 5' direction. Usually this strand is not shown, and is implicit.