expressing flag tag by itself - (Oct/22/2013 )
I was wondering if it's possible to express flag tag all by itself. I was thinking about putting the flag tag (with a kozak sequence in the beginning and a transcription terminator in the end) downstream of a promoter and upstream of a poly-A signal. Would this lead to expression of a short peptide that could be recognized by antibody?
Short answer is yes, however, the peptide will be tiny (6 kDa I think) and as such quite hard to detect by conventional western blotting techniques. You may be better off using a 3x flag tag.