Need help to identify bands in AGE result - (Mar/10/2014 )
i couldn't identify whether it is having smearing or bands,according to some of my laboratory seniors,some said is bands while some said it just having smearing result...
-fam-
These look like very short fragments, probably primer-dimers. It's not a specific product, unless it is perhaps a very small fragment.
-phage434-
The product size is compatible with the DNA ladder which around 227-272bp,and when I checked on NCBI primer BLAST,it targeted to specific primers: PIN1and GAPDH ,perhaps still got other factors to cause me get the failed result?
-fam-
If you expect short fragments, you should use a lower percentage gel and run the gel longer to get better resolution.
-phage434-