Protocol Online logo
Top : New Forum Archives (2009-): : PCR, RT-PCR and Real-Time PCR

Message Amount in qPCR - (Mar/21/2012 )

As you know when doing qPCR, you do a ratio of some housekeeping gene Ct to the Ct of your target message and compare levels between changes in that message over time, induction, etc. I want to know if anyone has data on the amount of message of GAPDH preferably in HEK293 cells? Is it 50 copies per cell? 1000 copies per cell? Just a mere ratio will not do it for me.



I'm affraid that can differ between batches of cell line. You can do absolute quantification to find out on your sample.