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cDNA concentration of qPCR - (Mar/20/2012 )

Which the minor concentration of cDNA that can I use in qPCR?
And which would the best concentration?


you need to look at the manual of your DNA polymerase. It must recommend a certain amount. The lowest could be 1 pg, but normally people use higher concentrations. we normally keep it below 1 ug because usually above that gives us smear on the gel.