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tissue homogenization problem - (Jun/25/2014 )

Hello,

 

I want to isolate RNA to use Tripure Reagent. I have 7 mg tissue and I used 700 ul Tripure Reagent. For homogenisation, I used the following product.

http://www.sigmaaldrich.com/catalog/product/sigma/z359971?lang=en&region=US

 

I done it roughly 20 min. but have incomplete sample homogenization.

 

Do you have any idea about this issue?

-medibio-

what kind of tissue? some are harder to homogenize than others.

-mdfenko-

It is biopsy material and has mucosa not very much lipids,...

-medibio-

lung? kidney? brain? with what tissue are you working?

-mdfenko-

pancreas 

-medibio-

did you follow the instructions for the use of tripure?

 

the homogenizer you're using may not be giving you proper shearing (they recommend teflon-glass or polytron).

-mdfenko-