tissue homogenization problem - (Jun/25/2014 )
Hello,
I want to isolate RNA to use Tripure Reagent. I have 7 mg tissue and I used 700 ul Tripure Reagent. For homogenisation, I used the following product.
http://www.sigmaaldrich.com/catalog/product/sigma/z359971?lang=en®ion=US
I done it roughly 20 min. but have incomplete sample homogenization.
Do you have any idea about this issue?
-medibio-
what kind of tissue? some are harder to homogenize than others.
-mdfenko-
It is biopsy material and has mucosa not very much lipids,...
-medibio-
lung? kidney? brain? with what tissue are you working?
-mdfenko-
pancreas
-medibio-
did you follow the instructions for the use of tripure?
the homogenizer you're using may not be giving you proper shearing (they recommend teflon-glass or polytron).
-mdfenko-