Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Biology

Tissue Preservation - (Jul/02/2014 )

I generally preserve specimens (whole fish) in ethanol to preserve tissue for DNA extraction to use in PCR reactions. I am out of ethanol at the moment but can borrow some Methanol Anhydrous (ultra pure grade). Will this preserve the tissue identically to ethanol, for purposes of DNA extraction?


As far as I know methanol works as good as ethanol, and even penetrates tissues better and faster (lower molecular weight). The only problem will be the toxicity of methanol, so you have to work carefully.

No idea if pure or diluted methanol is better.