Incompatible restriction sites - Will E.coli ligate for me? (Jan/14/2010 )
One of the restrictions sites of my fragment is common with that of the vector, but not the other. Can I still transform competent cells with this incompletely ligated molecule? Will E.coli ligate the other half for me? I am just not sure about this. Thank you.
Sean
-seanisaken-
seanisaken on Jan 15 2010, 12:46 AM said:
One of the restrictions sites of my fragment is common with that of the vector, but not the other. Can I still transform competent cells with this incompletely ligated molecule? Will E.coli ligate the other half for me? I am just not sure about this. Thank you.
Sean
Sean
ligation cannot occur b/w incompatible sites..........e.coli will not do it for you.
-DRN-
DRN on Jan 14 2010, 10:42 AM said:
seanisaken on Jan 15 2010, 12:46 AM said:
One of the restrictions sites of my fragment is common with that of the vector, but not the other. Can I still transform competent cells with this incompletely ligated molecule? Will E.coli ligate the other half for me? I am just not sure about this. Thank you.
Sean
Sean
ligation cannot occur b/w incompatible sites..........e.coli will not do it for you.
Thank you, DRN. I will proceed to order some linkers then.
-seanisaken-