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How Much BrET? - In TAE (Jan/19/2010 )

Hi everybody!

Just a small question, I want to reduce BrET use in my experiments and as we have only 1 microware used also for other people and things i prefer to use BrET on my TAE running buffer instead on gel.

In your experience wich is the smallest concentration of BrET I can use on my buffer in order to have a good DNA view?

Thanks a lot!


I usually use ~10ng of Et-Br for every ml of TAE.


Many Thanks!