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Promoter "optimization" - (Oct/07/2010 )

I was ask to optimize the promoter that I am using in my vectors. Any free guide or step by step manual to follow? I really don't know what to do!

Thanks

-MarcoVR-

What do you mean optimize? Is it mammalian, bacterial or other?
For what purpose do you need it?
Is it not expressed well in experiments?

Please be more specific.

-meggie82-