Cell-cycle independant qRT-PCR internal control - anyone has experience doing this? (Oct/06/2009 )
I am currently working on qRT-PCR, studying differential gene expression of human cells from diferent cell cycles. I am wondering which internal control should i use? as normal housekeeping genes such as GAPDH, b-actin may vary with different cell cycle.
Has anyone come across this?any suggestions of genes to be used in this case? (cell-cycle independant -internal control)
how about GUS?