qRTPCR using taqman - (Jan/04/2012 )
In qRTPCR using taqman, do we need to design both primers and probe. I ill be using Taqman expression assay mix. Please help as Iam totally new to this taqman thing..
-emailsonica-
Try to find something about primer and probe design and then use design software like Primer Express (you should have it if you have ABI machine) , or free aplication like primer3.
-Trof-
Thanks for replyin bt in taqman qrtPCR do we hav to design for both primer pairs n probe? I thought we just hav to design for probe...
-emailsonica-
Sure, you have to design both, for a probe to anneal, your specific region must be amplified with primers.
And please use all the letters, it's really difficult to read.
-Trof-
thanks. ok i will...
-DDLJ-