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Two stop codons - (Nov/18/2009 )

I have a pcr product with a start and stop codon....its inserted into a pCMV vector that has a signal sequence at the c terminal if i express it will the expression stop at the stop codon in the gen or at the stop codon after the signal sequence.... I want the signal sequence to fuse with my protein.......

Pls help asap


translation will stop at the first stop codon the ribosome encounters, so if your PCR product contains a stop codon at the end, translation will stop there and you will not get a fusion with your signal sequence in the vector.