Two stop codons - (Nov/18/2009 )
I have a pcr product with a start and stop codon....its inserted into a pCMV vector that has a signal sequence at the c terminal end...now if i express it will the expression stop at the stop codon in the gen or at the stop codon after the signal sequence.... I want the signal sequence to fuse with my protein.......
Pls help asap
translation will stop at the first stop codon the ribosome encounters, so if your PCR product contains a stop codon at the end, translation will stop there and you will not get a fusion with your signal sequence in the vector.