yeast two hybrid problem - (Nov/25/2009 )

Hey everyone:
I have some troubles in my yeast two hybrid experiments. Becasue my bait is self-activation , even after contransformed with prey, should all the results turn blue in Theoretically? But when i cotransformed my bait and preys, the result is not all turn blue. （the same bait vs several preys） Why?

shashababy on Nov 25 2009, 04:42 AM said:

the prey interacts to inhibit autoactivation?

is your bait a transcription factor?

eldon on Nov 25 2009, 03:50 PM said:

my bait is a 35AA peptide. This peptide can be autoactivation , either it fusioned to AD or BD. more interestiong, it can be autoactivation, even without AD or BD. too strange. there is no such short transcription factor reported before..........................

my bait also had self activation. by the domain analysis, i found that my protein has one SATA domain. In general, this domain has the function as one transcription factor. could i continue to do y2h via my protein