Removing sequencing migration artefacts - (Apr/13/2010 )
Has anyone had success using additives in sanger sequencing reactions to remove sequence fragment migration anomalies/artefacts
what kind of artifacts?
what are you seeing?
on what are you visualizing the reaction (machine, gel,...)?
some standard pcr additives (eg- betaine, ethylene glycol, propylene glycol, dmso, etc) work with sequencing difficult templates.