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T7 terminator-modification - (Nov/17/2010 )

Hi, guys,
I have a question about using pET-40b(+) vector. I plan to introduce a BlpI site into MCS. Unfortunately, there is an existing one in the T7 Terminator primer region, which is just before the T 7 Terminator sequence. Can I blunt the current BlpI site within T7 Terminator primer region without affecting the function of expression? By doing that, I can introduce a new BlpI site into MCS region.
Thanks for your advice.


Should be no problem! ...the functionality should be not affected to my knowledge!



Hi, pDNA,
Thanks man. I will try and let you know if it works or not.