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Increasing hybridisation efficiency using Cytoclear - removing troublesome cytoplasm (Nov/08/2010 )

hybridisation with radiolabelled and non-radiolabelled probes on chromosomes embedded in cytoplasm is low due to the weak penetration of the probe during hybridisation. I have discovered that we can improve the efficiency of the hybridisation by washing the cell suspension in Procell Cytoclear before slides are processed for hybridisation. I highly recommend the method. Our vendor is Rainbow Scientific, but I am sure others distribute the product. Hope tjis tip helps others?


Clear solution : dissolve 100 µg/ml pepsin in 0.01 N di HCl containing 0.1 % di Tween-20.
For specific application protocols ask to

-Zagami Francesco-