Protocol Online logo
Top : New Forum Archives (2009-): : PCR, RT-PCR and Real-Time PCR

is it necessary to do DNases treatment? - (Dec/14/2009 )

i got low amount of rna for all my samples (less than 10ug), but got to save them for real time pcr and microarray. i heard it is important to do dnases treatment of sample before real time/conventional pcr to avoid genomic contamination, but after dnases treatment, the sample should be clean up which i think my rna will be lost some in this step. I design my primers span exon-exon junction, will that help to avoid genomic dna amplification if i don;t dnases my sample? thanks

-wntiong-

If you have exon-exon spanning primers then there's no need to do DNase treatment. 10ug? per uL or?

Chris

-chrisbelle-

chrisbelle on Dec 16 2009, 04:47 PM said:

If you have exon-exon spanning primers then there's no need to do DNase treatment. 10ug? per uL or?

Chris


1ug/ul. need 10 ug in 10 ul for microarray. due to sample scarcity, hard to get this amount for my samples.

-wntiong-