Can normal sequencing services sequence >1kb product? - sequencing (Jun/16/2010 )
I have recently amplified some PCR amplicons with more than 1kb side band and I have cloned them in plasmid and send them for sequencing (both end) in a local private sequencing services company. However, one of my samples (around 2kb) did not get overlapping (missing of 132bp in the middle region) after I have try to align both end results and counter check them with BLAST.
My question is can normal sequencer machine sequence more than 1kb product?
Todays maximum is around 1000 bp, depending on the capillary length, polymer type, sequencing kit and template quality. You should design another sequencing primer in the middle of your sequence to get a 100% coverage.