QPCR/DDCT Method/ Statistics - (Jun/22/2010 )
I need your help.. I did a QPCR using the DDCt method and I would like to compare the 5 RQ values (actually i would like to compare 5 numbers) that I got. How can I compare the RQ values for statistical significance? I did not repeat the experiment so I can get means.
hi, what are these 5 samples? different treatments? i would say you need biological replicates within your different treatment groups (at least 3) to find out first the variance within one treatment group in order to compare different groups.
tea-test on Jun 23 2010, 12:09 PM said:
hi, thanx for your answer.. yes, my samples are tissues treated differently. i do have e biological replicates within my groups.